Objective To provide references for clinical rational use of Chinese patent medicine. Methods Totally 387 specifications of Chinese patent medicine in our hospital were analyzed, and were judged by pregnancy contraindication according to China Pharmacopeia (2010 edition). Results The 387 Chinese patent medicines involved 32 prohibited pregnancy ingredients, 1 taboo ingredient. In 89 Chinese patent medicines contraind prohibited ingredients, 33 marked prohibited ingredients, 18 marked contraindicated ingredients, 14 marked cautious ingredients, and 24 marked without-contraindication ingredients. 68 Chinese patent medicines contained pregnancy contraindication labels but without prohibited ingredients. Conclusion A unified standard of pregnancy contraindication should be established;researches on pregnancy contraindication should be strengthened;medicine specifications should be standardized and perfected.

Genetic factors are the important reasons for birth defects.Among genetic factors,chromosome abnormality is the most common.Prenatal diagnosis can effectively avoid fetus of abnormal chromosome being born.Noninvasive prenatal diagnosis has more advantage than invasive prenatal diagnosis,such as non-invasive,painless and safe.It is bound to become the main method of prenatal diagnosis.However,non-invasive prenatal diagnosis is still in its infancy stage and the accuracy and sensitivity of detection are pending and need further clinical validation.This article will present a variety of non-invasive prenatal diagnostic methods using cell-free fetal DNA (RNA) and their advantages and disadvantages will be discussed.

Incidence and mortality of colorectal cancer has increased significantly in recent years. Screening for colorectal cancer is the most effective method to decrease mortality. Colorectal adenoma is the precancerous lesion of colorectal cancer and can be detected through colonoscopy, which is the crucial in the early diagnosis and early treatment for colorectal cancer. The first step of screening is the selection of target population and the second step is colorectal examination. The selection of candidate for screening has direct effect on the efficacy of screening. The methods in common use include fecal occult blood test, questionnaire for high risk factors of colorectal cancer, colonoscopy, sigmoidoscopy, and CT virtual colonoscopy. Among those, colonoscopy is the most reliable method and widely used in the screening for colorectal cancer.
China ; Colonoscopy ; Colorectal Neoplasms ; diagnosis ; Early Detection of Cancer ; methods ; Humans ; Mass Screening ; methods ; Occult Blood

Antibodies, Monoclonal, Humanized ; administration & dosage ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Bevacizumab ; Capecitabine ; Chemotherapy, Adjuvant ; Colonic Neoplasms ; drug therapy ; surgery ; Deoxycytidine ; administration & dosage ; analogs & derivatives ; therapeutic use ; Disease-Free Survival ; Fluorouracil ; administration & dosage ; analogs & derivatives ; therapeutic use ; Humans ; Leucovorin ; administration & dosage ; Organoplatinum Compounds ; administration & dosage ; Semustine ; therapeutic use ; Survival Rate ; Vincristine ; therapeutic use

Objective To evaluate the significance and expression of the GDNF/GFRα1/RET genes in distal rectum with Congenital Anorectal Malformations(ARMs) Methods Specimens were collected from resected colon which is 3 cm away to the anus and the distal of rectum in 12 ARMs patients. Haematoxylin and Eosin (HE) staining, immunohistochemical (IHC) staining and reverse transcription PCR (RT-PCR) were used to test RET, GDNF and GFRα1 expression in the differ-ent site of samples with ARMs. Results Expression of ganglia and RET, GDNF and GFRα1 were all negative in distal rec-tum in 12 ARMs patients. Ganglionic cells were found in tissues 3 cm away from the anus where RET, GDNF and GFRα1 expression were also positive in those ARMs patients. Expression of RET, GDNF and GFRα1 were strong positive in middle and low imperforate anus indicated by brown color and week positive in high imperforate anus indicated by yellow color. One case of faeces contamination and one case of loose motion without anal incontinence were found in post-op follow up of high ARMs patients. By contrast, one case of rectal mucosa prolapse and one case of occasional faeces contamination which recov-ered with hip bath were found in post op follow up in middle or low ARMs. Conclusion The unsatisfactory anorectal func-tion is possibly related to the decrease or lost of neurotrophic factors (GDNF/GFRα1/RET) and ganglia in the myenteric plex-uses post plastic surgery in ARMs patients.

Epstein-Barr virus (EBV) remains one of the most important pathogens in children,which usually causes acute EBV infection,chronic active EBV infection,hemophagocytic lymphohistiocytosis,lymphoma and other cancers.Therefore,effective laboratory diagnosis is of importance in diagnosis,surveillance,and control of EBV-associated disease.Currently,a variety of detection methods have been applied in clinical laboratories and typical diagnostic assays are always focusing on EBV-DNA and serological tests.Different assays vary in different purposes and applications,which seems to be difficult to choose an appropriate technique.This article aims at systematically analyzing clinical significance of these assays and evaluating the immune functions of EBV infection and the detection of susceptibility genes of EBV-related disease.

Objective To evaluate the change of left ventricular(LV) function by real-time three dimensional echocardiography(RT-3DE) and to explore the clinical value of change of serum adiponectin level in patients with metabolic syndrome (MS).Methods One hundred and twenty patients were divided into MS group and control group according to the diagnostic standard of the nternational Diabetes Federation.Transthorasic 2D ultrasound and RT-3DE examination were performed in all study objects, and serum adiponectin levels were measured.Results Parameter index [including Tmsv16-SD, Tmsv12-SD,Tmsv16- Dif, Tmsv12-Dif, Tmsv16-SD (％), Tmsv 12-SD (％), Tmsv16-Dif (％), Tmsv12-Dif (％)] of 17segment time to minimal systolic volume in MS group was more than that in control group (all P ＜0.05).Some regional systolic index of LV and serum adiponectin level were less than that in control group (all P ＜0.05).Compared with the control group, E/E' was lower (P ＜ 0.05).There were significant relations between serum adiponectin level and E/A of mitral valves, E'/A' of mitral annulus and E/E'(r = 0.368,0.493,and -0.347 respectively, P ＜0.05).Conclusions RT-3DE can rapidly evaluate LV systolic and diastolic synchrony in MS patients.E/E' show an excellent correlation with serum adiponectin level,and it may be a new promising method for routine evaluation of LV diastolic function.

Objective To investigate the effects of bisphenol A on SD rat Sertoli cell function and the injury mechanism. Methods SD rat Sertoli cells were treated with bisphenol A at different doses,and the control group,solvent control group were set. Sertoli cell proliferation,cell cycle and PCNA,Vimentin protein expression. were observed. Results The survival rate of Sertoli cells in the treated groups was significantly lower than the control group (P

Objective To study the relationship between hyperferritinemia and early metabolism disturbance of sugar and Lipid.Methods 382 people in health examination from Hangzhou First People's Hospital were divided into hyperferritin group(A group)and hypoferritin group(B group)according to the level of ferritin.Blood fat,fasting blood glucose(FBG),uric acid(UA),hematoglobin(Hb),red blood cell count(RBC),hematocrit(HCT),mean corpuscular-hemoglobin concentration(MCHC)were determined,and adiposis hepatica was judged by ultrasonic inspection,then we analyzed by meams of statistics.Results The result showed that TG,LDL,HDL,ApoA1,ApoB,FBG,UA,Hb,RBC,HCT,MCHC had significant deviation between two groups.Ferritin was positive correlated with TG,TC,LDL,ApoB,Hb,RBC,HCT,and negative correlated with HDL,ApoA1.The incidence of adiposis hepatica in A group was obviously higher than B group.Conclusions Probably,ferritin was one of the markers of early metabolism disturbance of sugar and Lipid.

AIM: To discuss the clinical treatment of bacterial conjunctivitis, and summarize the treatment experiences. METHODS: Totally, 124 cases with bacterial conjunctivitis in the department of ophthalmology from October 2012 to April 2014 were selected as the research objects. According to the diagnosis points, they were classified. After symptomatic drug treatment, the efficacy and adverse reactions were observed.
RESULTS: The total effective rate of 124 cases after treatment was 96. 8%, after treatment, patients with tears, conjunctival congestion, secretions and pain scores were significantly lower than those before treatment ( P<0. 05). The total score after treatment was (0. 36±0. 24) in this group, there were 6 cases ( 4. 8%) of adverse reactions.
CONCLUSION: The efficacy of classification symptomatic treatment for bacterial conjunctivitis is significant and worthy of clinical reference.