Objective To observe the clinical effect of small dose mifepristone combined with traditional Chinese medicine in the treatment of uterine gland disease.Methods 76 patients with uterine gland disease were selected,they were divided into the observation group(n =38) and control group(n =38) by using random number table method.The control group was treated with small dose of mifepristone,while the observation group was treated with small dose of mifepristone combined with traditional Chinese medicine.The clinical curative effects of the two groups were compared.Results After treatment,in the observation group,the pain degree of patients [(21.30 ± 6.70) points],influence activity level [(17.30 ± 12.00) points],activity loss [(1.80 ± 0.70) spots] and grades of dysmenorrheal [(0.60 ± 0.20) stages] were lower than those in the control group,the differences were statistically significant (t =5.645,7.023,4.932,3.994,all P ＜ 0.05).After treatment,in the observation group,the uterine volume [(56.20 ± 2.00) mm3],CA125 level [(32.10 ± 2.10) U/mL] were lower than those of the control group (t =4.024,4.413,all P ＜ 0.05).The incidence rate of adverse reaction of the observation group was 7.89％,which was significandy lower than that of the control group,the difference was statistically significant (x2 =14.757,P ＜ 0.05).The patients were followed up for 8 months,the recurrence rate of the observation group was 5.26％,which was lower than 26.32％ of the control group,the difference was statistically significant (x2 =16.678,P =0.000).The menstrual recovery ratio less than or equal to three months of the control group was 81.58％,which was signifi candy higher than 63.16％ of the control group,the difference was statistically significant (x2 =8.484,P =0.003).Conclusion In the treatment process of adenomyosis,the small dose of mifepristone combined with traditional Chinese medicine can alleviate the clinical symptoms,reduce the pain and reduce the volume of uterus,and it has less adverse reaction,safe and reliable,it is worth of clinical application.

Objective To study the imaging characters and diagnostic value of spondylolysis and spondylolisthesis by usingthree-dimensional reconstruction of spiral CT. Methods 37 patients with spondylolysis were scanned by spiral CT.Various reconstruction methods were applied on the data acquired. Results The axial CT scans achieved good resolution on showing hyperostosis,fibrous callus and pathological changes of spinal disc.Spondylolysis was all displayed in the MPR/MIP of sagittal,reverse gantry angle and CPR.MIP,MPRand CPR could provide better resolution in display of spondylolysis than SSD did,while SSD was better in display of hyperostosis andspondylolisthesis. Conclusion The three-dimensional reconstruction of spiral CT is a good method for the radiographic evaluation ofspondylolysis and its relative changes.This technique can provide reliable informations for planning clinical therapy.

Objective To explore the technique of double spiral CT cerebral angiography in order to improve the quality of CTA.Methods All 62 cases underwent cerebral CTA,including vascular lesions in 30,normal in 20 and others in 12.All were scanned with GE Hispeed NX/I CT,three-dimensional models were used in images reconstruction.Results The branches of 1~4 grades of cerebral arteriae could be displayed well in all cases,some cases could also be visible up to 5th grades.All cerebral vascular lesions could be showed by CTA and the same as DSA.Cerebral tumor could be demonstrated with tumor stain.The image quality would be better when the pitch was smaller with the same layer.Conclusion The factors that influence on the quality of CTA images are a lot of,but mostly are concerned with the pitch,contrast volume,injection rate and delayed time.

Nephrocalcinosis is often accompanied by kidney stone disease. In recent years, more and more nephrocalcinosis has been found to be caused by a monogenic disease, and its pathogenesis has not been fully elucidated. With the development of molecular genetics, more than 30 genes have been found to be the causative genes of nephrocalcinosis. At the same time, with the widespread development of genetic testing technology, more patients have received early diagnosis and timely intervention. This article reviews the clinical and basic research progress of monogenic nephrocalcinosis.

Objective To investigate the X-ray,CT and MRI features of peripheral primitive neuroectodermal tumors (pPNETs).Methods 14 patients were pathologically diagnosed with pPNETs.The imaging of X-ray (twelve image),Computed Tomography(eleven image) and Magnetic Resonance Imaging(five image) were retrospectively analyzed.Results Of 14 patients,there were two sites of tumour:soft tissue( eight patients) and bones( six patients).For tumoura of the soft tissue region,the appearance of X-ray and CT imaging showed equal or lower density soft mass without dear boundary.In some cases,tumours invaded adjacent bones.The appearance of MRI imaging showed large heterogeneous masses inside soft tissue without clear boundary.Tumours displaced adjacent cortical bone and cavitas medullaris.All masses were heterogeneous enhancement on contrast images.For tumours of bone region,X-ray and CT scan showed large scale osteolysis osteoclasia in bone surrounding with soft tissue masses of various size.The lesions were equal or lower signal on T1 WI.All masses were intermediate or hyperintense signal with heterogeneous midrange or obvious enhancement on T2WI.Conclusion Imaging characteristics of pPNETs were described.X-ray,CT and MRI were useful in understanding the property,extent and staging of tumor and very useful in the diagnosis.

Objective To explore how Vibrio vulnificus (Vv) invades dendritic cells (DC) and induces acute necrosis of DC via toll-like receptor (TLR) 2 and 4 pathways.Methods Vv 1.1758 strain and DC 2.4 mixed culture model was established,observed the infection rates of DC with optical microscope,the location of Vv and structural changes of DC by transmission electron microscope.The expression levels of TLR2 and TLR4 mRNA were determined by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) and tumor necrosis factor-α (TNFα) protein titers were measured by enzyme-linked immunosorbent assay (ELISA).DNA ladder qualitative test was used to detect cell apoptosis,while flow cytometry was used to quantify cell apoptosis and necrosis rates.Statistical analysis was done by chi-square test and one-way ANOVE.Results The infection rates of DC after 0.5,1,2,4 and 6 h of mixed culture were (7.8±0.8) ％,(13.9± 1.1) ％,(34.6±4.9) ％,(77.8± 10.2)％ and (95.8 ± 13.1)％,respectively.Vv was generally located in the internal cell membrane of DC 2.4.After 2 h co-culture,nuclear chromatins of DC became active and intranuclear apoptosis bodies appeared.After 4 h,cytoplasmic vacuoles appeared,chromatin gathered,and cell membranes were seriously damaged.After 6 h,mitochondria was highly swelled and distorted,and cell apoptosis and necrosis occurred.TLR2 and TLR4 mRNA levels reached peak values after co-culture for 0.5 h; TNF-α level began to increase at 1 h (P＜0.05) and reached peak values at 2 h.DNA Ladder electrophoresis presented scouring necrosis after 2 h culture and apoptotic bands appeared between 720 bp and 900 bp after 4 to 5 h culture.Early apoptosis rates of DC after 2,4 and 6 h culture were (3.1±3.8)％,(7.8±4.7)％ and (12.7±8.2)％,and necrosis rates of DC were (16.7±12.5)％,(41.6±25.9)％ and (75.5±33.6)％,higher than that of control group (all P＜0.05).Conclusions Vv infects DC and induce DNA degradation through up-regulated expression of TLR2 and TLR4 and increasing of TNF-α inflammatory mediators.During cell degradation,apoptosis and necrosis coexist,while necrosis is predominant.

Primary hyperoxaluria (PH) are important causes of kidney stone and chronic kidney stone disease in children. Recurrent kidney stone disease and nephrocalcinosis should alter the physician to the possibility of an inborn error of metabolism as the underlying cause. Unfortunately, the lack of recognition of genotype and phenotype of PH resulted in an unacceptable delay in diagnosis and treatment, sometimes with grave consequences. This paper review the characteristics of genotype and phenotype, genotype-phenotype correlation, current treatment and future gene therapy of PH.

OBJECTIVETo study the effect of berberine on the release of nitric oxide (NO) by rat intestinal mucous microvascular endothelial cells (RIMECs) cultured in vitro for exploring the pharmacological mechanism of berberine in treating intestinal disease.

METHODSCultured RIMECs in vitro were identified adopting immunofluorescent stain with factor VIII-related antigen. NO level in the supernatant of normal cell culture or cell culture treated with different concentrations of berberine was detected with colorimetry at the 3rd, 6th, 9th and 12th h and compared.

RESULTSCompared with that in the normal control group, NO level increased more obviously in the berberine treated groups, and the best effect was shown in the 5 microg/mL berberine treated group.

CONCLUSIONOne of the important pharmacological mechanisms of berberine might be through promoting the endogenous NO release to induce endothelium-dependent dilatation of microvascular in intestinal mucosa, thus to improve the local microcirculation of intestine.

Animals ; Berberine ; pharmacology ; Cells, Cultured ; Colorimetry ; Endothelial Cells ; cytology ; drug effects ; metabolism ; Intestinal Mucosa ; blood supply ; cytology ; Nitric Oxide ; biosynthesis ; Rats ; Time Factors

Objective To evaluate the efficacy and safety of intra-arterial thrombolysis with recombinant tissue-plasminogen ac-tivator(rt-RA) for treatment of acute cerebral infarction (ACI) .Methods The randomized controlled trials (RCT ) of intra-arterial thrombolysis with rt-PA for treatment of ACI were selected from Cochrane Library ,Medline ,Embase and CBM by the computer re-trieval .A meta analysis of the data were analyzed by using RevMan 5 .1 software .Results A total of 473 patients in 9 selected RCTs were involved in the systematic review .Meta-analysis results disclosed suggested that the effectiveness of intra-arterial thrombolysis treated ACI group was better than that of intravenous thrombolysis group [RR= 1 .26 ,95% CI(1 .09 ,1 .45) ,P=0 .002] .No difference was observed between the two groups in intracranial hemorrhage and mortality rate .Conclusion The current evidence showed that ,the clinical effect of intra-arterial thrombolysis with rt-PA for treatment of ACI was obviously superior to that of intravenous thrombolysis ,but the result still needs to be confirmed by large-sample RCTs .

The paper summarized the successful experience such as preliminarily establishing cultivation mechanism of manufacture-learning-research cooperation for postgraduates of science of Chinese pharmacology of Guangzhou University of Chinese Medicine,ensuring innovation ability training for postgraduates,getting reasonable configuration for social education resources,and put forward existing problems in traditional Chinese medicine system,such as the loose management and faulty evalution system.Suggestion is to futher perfect relevant rules and regulations,clear and definite responsibility of school and enterprise,reserch regularly to impove ideological education of postgraduates,etc.In the way,we can achieve the aim of promoting high-level talents of traditional Chinese medicine industry.