Objective To investigate the laparoscopic treatment for giant intraabdominal cysts with postoperative lower body edema. Methods Five cases of giant intraabdominal cysts were given an aspiration of 1 500 ~ 2 000 ml (mean, 1 800 ml) of fluid. The aspiration was performed percutaneously in 2 cases of hepatic cyst, and under direct vision through a 1.5~2.5 cm incision around the umbilics in 3 cases of lower intraabdominal cyst. After aspiration the cysts were excised (3 cases) or fenestrated (2 cases) laparoscopically. Postoperative lower body edema was treated with diuretics either orally (2 cases) or intravenously (3 cases). Results Symptoms completely disappeared after operation in 4 cases and were significantly improved in 1 case of polycystic liver. Follow-up for 3~12 months found no recurrence. The lower body edema subsided in 5~7 days after operation. Conclusions Aspiration of cystic fluid before laparoscopic operation and postoperative administration of diuretics could get good results for patients with giant intraabdominal cysts.

AIM: To study the discrepancy and it's significance of ATP binding cassette transporter(ABC1) and CD36 mRNA expression while activate nuclear receptor RRAR?,LXR?,RXR? in THP-1 cells. METHODS: Cultured THP-1 cells were induced to become macrophage by PMA at concentration of 40 ?g/L for 48 hours. Troglitazone, 22(R)-hydrocholesterol and 9-cis-retinoid acid(9-CRA),activators for PPAR?,LXR? and RXR? respectively, were incubated with the macrophage for 24 hours. Total RNA of these cells were abstracted and reverse transcriptional polymerase chain reaction were performed to inspect the expression of ABC1 and CD36 mRNA in the cells. RESULTS: Expression of ABC1 mRNA increased in the presence of troglitazone, 22(R)-HC and 9-CRA. CD36 mRNA also increased while PPAR? activated. Activation of LXR? showed almost no effect on CD36 mRNA expression, and expression of CD36 mRNA was decreased by 9-CRA.CONCLUSION: The discrepancies existing in ABC1 and CD36 mRNA expression while activate nuclear receptors PPAR?,LXR?,RXR? in THP-1 cells implyed that LXR?,RXR? were better targets to deal with to improve cholesterol reverse transportation.

Biliary atresia (BA) is a kind of disease of unknown etiology, characterized by progressive inflammation and fibrosis of obstructive biliary diseases. Kasai portoenterostomy is the only method to treat BA. However, about 80% of the patients treated by Kasai operation still need liver transplantation in the future. Many factors affect the survival of autologous liver in children with BA after Kasai operation, including the types of BA, laparoscopic Kasai surgery or traditional open surgery, patient’s age at surgery, condition of liver function, occurrence of cholangitis, jaundice clearance, using steroids and central hospitalization. This article reviews the factors that affect the survival of autologous liver in patients with BA after Kasai surgery.

Drug eluting stents (DESs) have revolutionized the interventional cardiology over the past decade since the first DES became commercially available in Europe in 2002. Compared to bare metal stents that are deployed to keep the vessel open by mechanical force, DESs have an additional function of reducing restenosis by the action of the drug on the target site. Coatings on the stent surface which ensure the maximum delivery of therapeutic agents to the target site with minimal systematic toxicity, also play an important role in adjusting the drug release profile. Coating material and technology not only affect the surface biocompatibility and the integrity maintenance during the implanting process, but also decide the way of drug delivering and transmitting from the coating. This paper reviews the basic principles of DES coating design, the categories of DES coatings, the commonly used clinical DES coatings and their efficiency in reducing restenosis, and finally provides the future perspectives for DES coatings.

Objective To find out the status of nosocomial infection management in hemodialysis sections, in order to encourage their continuous quality improvement and standardize their management Methods 41 hemodialysis sections with (≥) 3 dialyzers and independent operation were chosen for investigation. On-site investigation and environmental microbiology monitoring combined. Results The higher the level of the hospital, the better the nosocomial infection control in its implementation of the infection control measures. In terms of rules and regulations, incompliant secondary hospitals and private hospitals accounted for 56. 2% of hospitals of the same ranking; in terms of layout and processes, 75% are found incompliant; and various defects are found in the dialyzer reuse management and disinfection in tertiary and lower hospitals. As to hand hygiene of the staff, the incompliance rate is over 55%. As indicated in the results of microbial environment monitoring, the compliance rate of dialysis water is over 90%, that of dialysate is 78.6% ～ 86.2%, and that of hand disinfection is 56.6%～78.6%.Conclusion It is imperative to strengthen the supervision of secondary hospitals and private hospitals,and to train their staff in the knowledge of hand hygiene and nosocomial infection in hemodialysis rooms.Hospitals found with poor infection management will be encouraged to take issue-specific actions for rectification.

Objective To investigate the family function and features of parental attachment in children with tic disorders ,and to provide evidence for their family intervention. Methods Fifty three children with tic disorders and eighty five normal controls were investigated by family adaptability and cohesion scales, second edition, Chinese version (FACES Ⅱ-CV) and attachment security scale(Kems Security Scale). Results Actual cohesion scores,actual adaptability scores and ideal adaptability scores of the tic disorders children were all lower than those controls((66.87 12.53) vs (72. 14 11. 05) , (44. 19 9.70) vs (49.01 9.19), (52. 38 9. 10) vs (57.10 8.60), P<0.05, P<0.01); the ratio of the Disengaged type of cohesion in the tic disorders children was higher than that of normal controls(25.5% vs 5.9% , P<0.01) ;the ratio of the Rigid type of adaptability was higher than that of normal controls(55.3% vs 25.9% , P<0.01) ;the ratio of the Flexible type of adaptability was lower than that of normal controls( 12.8% vs 30.6% , P<0.05) ;the proportion of the type of extreme in the tic disorders children was higher than that of normal controls(40. 4% vs 23. 5% , P<0. 05). Father-dependence, mother-dependence, mother-intimacy, father-child attachment, mother-child attachment were all lower than those controls. In cases,positive correlation was found not only between actual cohesion scores and father-dependence, mother-dependence, mother-intimacy, father-child attachment, mother-child attachment, but also between actual adaptability scores and the above-mentioned five factors (0.291≤ r≤0.445,0. 301 ≤ r ≤0. 504). Mother-child attachment as an independent variable was separately leaded into the multiple progressively regression model for actual cohesion scores and actual adaptability scores(P<0.01). Conclusion The family function and parental attachment security of the tic disorders children were lower than the controls.

Objective To explore the compatibility rules of acupoints and meridians in acupuncture-moxibustion treatment for vertigo, by using the association rules of data mining.Method The relevant data were collected from literatures about acupuncture-moxibustion in treating vertigo from the Pre-Qin Period to Qing Dynasty to establish a database. Then the involved acupoint and meridian groups were further extracted by using association rules of data mining.Result Shangxing (GV 23) and Fengchi (GB 20), Fenglong (ST 40) and Jiexi (ST 41), Hegu (LI 4) and Fengchi (GB 20), Fenglong (ST 40) and Fengchi (GB 20), Waiguan (TE 5) and Zulinqi (GB 41), Hegu (LI 4) and Fenglong (ST 40), Hegu (LI 4) and Jiexi (ST 41), Yanggu (SI 5) and Zulinqi (GB 41), Yanggu (SI 5) and Fengchi (GB 20), Shenting (GV 24) and Shangxing (GV 23) are the most commonly used acupiont pairs; the Governor Vessel, Gallbladder Meridian and Bladder Meridian are the most commonly used meridian groups.Conclusion It’s reasonable to use association rules to study the ancient acupuncture-moxibustion literatures, and the results about acupoint and meridian groups provide references for both clinical practice and experimental researches.

Dysregulated cell cycle-mediated cell transformation and uncontrolled cell growth are some of the fundamental biolog-ical features of malignant tumors. Thus, understanding the mechanism(s) involved in the cell cycle will facilitate the discovery of the na-ture of tumor initiation and progression as well as the molecular mechanism of tumor malignancy. Consequently, biomarkers and drug targets for early diagnosis and treatment would be obtained. This study was designed to analyze in detail the key regulators of cell cycle and their importance in carcinogenesis and clinical prevention, diagnosis and treatment, and anti-cancer drug discovery. The biological impact of the dysregulated cell cycle on tumor occurrence and malignancy development is systematically described. The theoretical and practical significance of the cell cycle regulation in tumor diagnosis and therapy are also provided.

OBJECTIVE: To observe the transcranial Doppler ultrasonography and X-ray changes of cervical vertigo patients caused by cervical instability before and after the manipulation treatment in supine position. METHODS: Sixteen cervical vertigo patients who underwent supine manipulation treatment were reviewed retrospectively. After the treatment, the changes of transcranial Doppler ultrasonography of cervical vertebral artery and X-ray of cervical spine were observed. RESULTS: Before the treatment, the mean blood flow velocity of vertebral artery was (48.6+/-3.1) cm/s, much higher than that of the normal group (P<0.05), the means of the horizontal displacement of C3,4 and C4,5 were (3.18+/-0.42) mm and (3.46+/-0.26) mm respectively. After the treatment, the blood flow velocity and the C3,4 horizontal displacement decreased significantly (P<0.05). The effective rate of the manipulation treatment was 100%. CONCLUSION: The manipulation treatment is an effective method for cervical vertigo patients caused by cervical instability, and the effect is correlated with the improvement of the blood flow of cervical artery and the restoration of cervical stability.