Objective:To explore the inflammatory responses of macrophages treated with Helicobacter pylori. Methods:Cytokines IL-23,IL-10,TNF-α and IL-8 in cell culture supernatant of macrophages stimulated with Helicobacter pylori were determined by ELISA kits,and the expression of intracellular proteins NOS2 and COX2 in Helicobacter pylori treated macrophages was analyzed by Western blot. Then,the apoptosis of Helicobacter pylori stimulated macrophages was detected by flow cytometry. Results:The secretion of cytokines IL-23,IL-10,TNF-α and IL-8 in the culture supernatant of Helicobacter pylori treated macrophages increased significantly (P<0. 05),and the expression of NOS2 and COX2 was enhanced evidently(P<0. 05). Meanwhile,helicobacter pylori could induce the apoptosis of macrophages obviously ( P<0. 03 ) . Conclusion: The inflammatory responses of macrophages treated with Helicobacter pylori would be promoted to inhibit or kill Helicobacter pylori,furthermore,Helicobacter pylori could induce the apotosis of macropha-ges.

Objective To study the quality standard of Folium Nelumbinis.Methods Flavonoids of Folium Nelumbinis were identified by TLC.The extraction conditions of flavonoids was studied and the content of total flavonoids was determined by ultraviolet spectrophotometry .Results Quercetin in Folium Nelumbinis was identified by TLC.The optimal extraction conditions were defined and the content of total flavonoids was about 13 %.Conclusion This method is simple and convenient, and is feasible for the quantitative determination of Folium Nelumbinis.