1.Expression of KPNA2 in hepatocellular carcinoma
Feng LI ; Zeyan PU ; Fangjiu LIU ; Rong WEI ; Yanli LIANG
International Journal of Laboratory Medicine 2014;(15):2031-2032
Objective To detect the mRNA expression of KPNA2 in hepatocellular carcinoma(HCC) tissue and to investigate its relationship with the clinicopathological features of HCC so as to provide the basis for predicting HCC infiltration ,metastasis and early treatment .Methods The HCC tissue and paracarcinoma normal tissue from 30 operative cases of HCC in the hepatobiliary surgery department of this hospital from January 2011 to November 2013 were collected and detected KPNA2 expression at the transcriptional level by RT-PCR .The correlation between the mRNA expression of KPNA2 with the clinicopathological factors of HCC was analyzed by consulting the medical record data .Results Among 30 cases of HCC ,the ratio of KPNA2 mRNA in the HCC tissue and the paracarcinoma normal tissue in 25 cases(83 .33% ) was up-regulated (grey value >2 times) .Therefore ,the KP-NA2 gene exhibited the high expression in the HCC tissue and low expression in the paracarcinoma normal tissue .Conclusion KP-NA2 is highly expressed in the HCC tissue and lowly expressed in the paracarcinoma normal tissue ;the high expression of KPNA2 is positively correlated with the stage and pathological grading of HCC ;KPNA2 may be used as an important indicator for early di-agnosing HCC and judging the malignant degree and potential metastais ability of HCC .
2.The current funding landscape of medical artificial intelligence research projects: an analysis of national natural science foundation of China from 2015 to 2019
Hongzan SUN ; Zeyan XU ; Zaiyi LIU ; Heqi CAO
Chinese Journal of Radiology 2021;55(6):661-666
Objective:To investigate the current funding landscape of medical artificial intelligence (AI) projects in National Natural Science Foundation of China (NSFC) from 2015 to 2019.Methods:From 2015 to 2019, AI-related projects in NSFC Medical Science Department were collected. Comprehensive analysis was performed in the projects information including year, title, supporting institution, fund type, research findings, etc.Results:NSFC has funded a total of 278 projects related to artificial intelligence, with the total funding amount of 139 million yuan. The number of projects and the funding amount were increasing year by year. Among these, 90% (249/278) were general programs and young scientist funds; 53% (148/278) of the projects were regionally distributed in Beijing, Shanghai and Guangdong; 66% (184/278) of the projects were imaging-related researches; the projects mainly focused on diseases with high incidence in China, including neoplastic diseases, cardiovascular and nervous system diseases.Conclusion:The AI-related projects funded by NSFC are characterized by rapid growth in number and fund amounts, wide coverage of disciplines, and diverse types of research diseases. However, the unbalanced distribution of regions, research fields, and supporting institutions demands more attention in future.
3.Associations between disorders in activities of daily living and heavy metal concentrations in elderly people
Tingjun LI ; Jiansheng CAI ; Ruiying LI ; Jie XIAO ; Zeyan YE ; Yuqian CHENG ; Zhe LIU ; Zhiyong ZHANG
Journal of Environmental and Occupational Medicine 2024;41(9):995-1003
Background Heavy metals may play an important role in environmental risk factors associated disorders of activities of daily living (ADL) in older adults. Objective To investigate the associations between plasma levels of six heavy metals (zinc, arsenic, cadmium, lead, manganese, and copper) and ADL disorders in older adults. Methods A cross-sectional survey was conducted from 2018 to 2019 among
4.Study on changes of blood electrolyte levels and assessment of mortality rate in patients with different degrees of craniocerebral injury
Jinsong DENG ; Guozhong GONG ; Wenli XING ; Tang LV ; Xianli TONG ; Youying LIU ; Zeyan PU
International Journal of Laboratory Medicine 2018;39(6):675-677
Objective To investigate the relationship between electrolyte level change with prognosis in the patients with craniocerebral injury.Methods A total of 360 patients with craniocerebral injury in this hospital during 2012-2015 were selected as the research subjects and divided into the mild craniocerebral injury group (171 cases),moderate craniocerebral injury group(104 cases)and severe craniocerebral injury group(85 cases) according to the Glasgow coma scale.The severe craniocerebral injury group was further divided into the high level blood sodium subgroup(73 cases)and stable level blood sodium subgroup(12 cases)according to the lev-el of blood sodium,meanwhile 70 persons undergoing healthy physical examination were selected as the control group.The plasma electrolyte levels(blood sodium,potassium,chloride)in each group were detected within 5 d after admission.Then the results were statistically analyzed.Results Compared with the control group,the blood sodium,potassium and chloride levels had no statistical difference between the mild and moderate craniocerebral injury groups(P>0.05).The blood sodium and chloride levels in the severe craniocerebral inju-ry group were higher than those in the mild and moderate craniocerebral injury groups,the difference was sta-tistically significant(P<0.01).The blood potassium level had no statistical difference between the mild,mod-erate and severe craniocerebral injury groups with control group(P>0.05).In the severe craniocerebral injury group,there were 58 cases(79.45%)of death in the high level blood sodium subgroup and 4 cases(33.33%) of death in the stable level blood sodium subgroup,the difference was statistically significant(P<0.01).Con-clusion Clinically monitoring the blood sodium level change in the patients with craniocerebral injury,espe-cially severe craniocerebral injury,is conducive to the disease recovery.
5.White-Matter Hyperintensities and Lacunar Infarcts Are Associated with an Increased Risk of Alzheimer's Disease in the Elderly in China.
Shuai YE ; Shuyang DONG ; Jun TAN ; Le CHEN ; Hai YANG ; Yang CHEN ; Zeyan PENG ; Yingchao HUO ; Juan LIU ; Mingshan TANG ; Yafei LI ; Huadong ZHOU ; Yong TAO
Journal of Clinical Neurology 2019;15(1):46-53
BACKGROUND AND PURPOSE: This study investigated the contribution of white-matter hyperintensities (WMH) and lacunar infarcts (LI) to the risk of Alzheimer's disease (AD) in an elderly cohort in China. METHODS: Older adults who were initially cognitively normal were examined with MRI at baseline, and followed for 5 years. WMH were classified as mild, moderate, or severe, and LI were classified into a few LI (1 to 3) or many LI (≥4). Cognitive function was assessed using the Mini Mental State Examination and the Activities of Daily Living scale. RESULTS: Among the 2,626 subjects, 357 developed AD by the end of the 5-year follow-up period. After adjusting for age and other potential confounders, having only WMH, having only LI, and having both WMH and LI were associated with an increased risk of developing AD compared with having neither WMH nor LI. Moderate and severe WMH were associated with an increased risk of developing AD compared with no WMH. Furthermore, patients with many LI had an increased risk of developing AD compared with no LI. CONCLUSIONS: Having moderate or severe WMH and many LI were associated with an increased risk of developing AD, with this being particularly striking when both WMH and LI were present.
Activities of Daily Living
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Adult
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Aged*
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Alzheimer Disease*
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China*
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Cognition
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Cohort Studies
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Follow-Up Studies
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Humans
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Magnetic Resonance Imaging
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Strikes, Employee
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Stroke, Lacunar*
6.Hypoxia-inducible factor-1 alpha effects on bone marrow mesenchymal stem cell mobilization in rats with acute myocardial infarction
Jinwei QI ; Jinglin CHENG ; Shu ZHOU ; Jingrong LI ; Xuexiang LI ; Qin YANG ; Hao ZHANG ; Jun WAN ; Yulin WANG ; Lixin ZHANG ; Yunyun CHEN ; Xiuxia XI ; Li YE ; Qian TANG ; Feng XU ; Yang JANG ; Leyi HU ; Zeyan LIU
Chinese Journal of Tissue Engineering Research 2014;(10):1579-1584
BACKGROUND:Increasing autologous stem cellmobilization is conceived to achieve effectively repair of cardiac ischemic injury. Therefore, it is important to seek a specific and effective mobilization agent. OBJECTIVE:To observe the effects of hypoxia-inducible factor-1α(HIF-1α) on bone marrow mesenchymal stem cellmobilization in myocardial infarction. METHODS:Left anterior descending artery was ligated to establish a rat model of acute myocardial infarction in 90 outbreeding Sprague-Dawley rats, and then the models were randomly divided into three groups. In HIF-1α-antisense oligonucleotide (ASODN) group, HIF-1α-ASODN was infused into the tail vein to restrain the expression of HIF-1αin infarcted ischemic tissue. In HIF-1α-missense oligonucleotide (MSODN) group or control group, an equal volume of HIF-1α-MSODN or saline was injected. RESULTS AND CONCLUSION:After 30 hours and 7 days of modeling, the number of bone marrow mesenchymal stem cells and expression of vascular endothelial growth factor in the peripheral blood of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. After 7 days of modeling, the expressions of HIF-1αprotein, vascular endothelial growth factor protein and mRNA in the ischemic myocardial tissues of the control group were similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. After 7, 14 and 28 days of modeling, the capil ary density in the ischemic myocardial tissues of the control group was similar to the HIF-1α-MSODN group, but significantly higher than the HIF-1α-ASODN group. These findings indicate that after acute myocardial infarction, high expression of HIF-1αexhibits a causal relationship with mobilization of bone marrow mesenchymal stem cells, initiating a series of self-healing process of myocardial tissues.