The seasonal dynamics of the soil microbial growth and soil net respir ation rate were studied in the imminent Heptacodium miconioides community T he results indicate that the numbers of bacteria and actynomices of rhizosphere soil or that of bacteria ,fungi and actynomices of non rhizosphere soil as well as net respiration rate of soil maintain similar seasonal dynamics in which dis play mono peak curves and their biggest values occure in September but the bi ggest nu mber of fungi of rhizosphere soil is in October The microbial numbers of soil,e s pecially that of soil fungi ,are greatly affected by the water content and tempe rature of soil The net respiration rate of soil closely relate not only with t h e water content and temperature of soil,but also with the soil microbial numbers of non rhizosphere soil which are mostly responsible for the net respiration r ate of soil

Objective:To establish a general method for the simultaneous determination of hydroxyphenyl esters and quaternary ammonium bacteriostatic agents in eye drops.Methods:The chromatographic analysis was per-formed on an Agilent C18 column(4.6 mm ×250 mm,5 μm)with 1％triethylamine solution(pH adjusted to 5.0 with phosphoric acid)as mobile phase A and methanol as mobile phase B.Gradient elution was performed at col-umn temperature of 40 ℃.The detection wavelength was 214 nm,the flow rate was 1 mL·min-1,and the injec-tion volume was 20 μL.Results:Methylparaben,ethylparaben,propylparaben,butylparaben,benzalkonium chlo-ride and benzalkonium bromide were 0.11-559.0,0.10-513.0,0.10-258.8,0.11-270.5,1.07-537.0 and 1.03-512.8 μg·mL-1,respectively.The linear range was good(r＞0.999).The average recoveries of meth-ylparaben,benzalkonium bromide and benzalkonium chloride were 104.7％(RSD=1.3％),102.6％(RSD=1.1％)and 100.9％(RSD=1.1％),respectively.The contents of bacteriostatic agent in 100 batches of eye drops from 36 varieties of 12 enterprises were determined,and the accurate results were obtained.Conclusion:This meth-od provides a reference for the content quality control and safety evaluation of bacteriostatic agents in eye drops.