Objective To explore the auxiliary efficacy of microbial ecological agents for elderly patients with acute pancreatitis.Methods Totally 144 elderly patients with acute pancreatitis were selected and randomly divided into the observe group and the control group (n=72,each).All patients received the regular treatment and enteral nutrition.Microbial ecological agents were added in enteral nutrition in the observe group.The levels of endotoxin (ET),tumor necrosis factor (TNF-α),interleukin (IL)-6 and C-reactive protein (CRP)in peripheral blood were determined and compared between the two groups.The clinical index and the occurrence of adverse reactions were observed.Results Compared with pretreatment,the levels of ET,TNF-α,IL-6,CRP were significantly reduced in both two groups after treatment.The levels of ET,IL-6,TNF-α,CRP were lower in the observe group than in the control group [(0.14±0.07)EU/ml vs.(0.31±0.12)EU/ml,(44.77±17.82)pg/ml vs.(63.25 ± 14.26)pg/ml,(0.15 ± 0.05)μg/L vs.(0.29 ± 0.14)μg/L,(108.33 ±11.84)mg/Lvs.(135.41±21.45)mg/L,t=14.722,43.824,7.071,5.811,respectively,allP＜0.05].The recovery time of symptoms and signs,the anal exsufflation time and hospitalization time were shorter in the observe group than in the control group [(25.8±4.8)h vs.(36.4±6.2)h,(30.9±6.7)hvs.(44.4±7.3)h,(24.9±3.2)hvs.(37.6±3.4)h,(6.9±0.4)dvs.(13.1±1.2)d,t=4.747,4.401,10.125,33.443,respectively,all P＜0.05].There were no statistically differences in death rate and the occurrence of adverse reactions between the two groups (x2 =0.15,0.64,both P＞0.05).Conclusions Microbial ecological agents are safe and effective as an adjuvant therapy for elderly patients with acute pancreatitis.It is worth promoting in clinical medicine.

Chalcone is a common scaffold in natural products with optimal properties and biological activities.In this study, we designed and prepared eight new coumarin-chalcone derivatives (5a-5h), and confirmed their structures by 1H NMR and 13C NMR. Their in vitro antifungal activity combined with fluconazole (FLC) against drug-resistant Candida albicans was tested by microdilution method.The results indicated that most chalcone derivatives showed good antifungal activity against drug resistant Candida albicans with FLC, particularly with compound 5g displaying better antifungal activity (MIC50 = 5.60 μg/mL) than FLC (MIC50 = 200 μg/mL) when combined with FLC, so, these derivatives could be used as synergists of antifungal drugs.

Objective To investigate the safty and feasibility of simultaneous minimally invasive technique for congenital heart disease (CHD) and pectus excavatum (PE).Methods From July 2006 to June 2011,6 children with PE associated with CHD were treated by simultaneous minimally invasive technique.They were 4 boys and 2 girls,aged from 4 years to 6 years 5 months ( average 5 years and 4 month),.The body weight were from 16 kg to 20 kg [ average ( 18.00 ± 1.79kg ].CT Hailer index were 3.9 - 5.0 ( average 4.35 ± 0.43 ).4 patients with ventricular septal defect (VSD) were treated by minimally invasive closure device,including 3 membranous VSD and 1 subaortic VSD.The defect diameter was 4 - 5 mm.2 cases with atrial septal defect (ASD) were treated using the same technique.the diameter of ASD was 12 - 16 mm.After minimally invasive heart surgery,Nuss operation was carried out in all 6 patients for their PE.Results All operations were successful and patients were extubated 5 - 11 h after operation [ mean ( 8.17 ± 2.04) h ].The pericardial mediastinal drainage was removed at 48 h postoperatively.There were no operative mortality and severe postoperative complications.1 patient had delayed wound healing and was successfully discharged.The plate was removed in 3 cases,and they all had satisfactory outcomes.Conclusion Simultaneous minimally invasive technique is a safe and effeictive mothod for treating pectus excavatum and congenital heart disease.

Objective To evaluate the effect of popliteal fossa fixed method to reduce the setup errors in patients with postoperative cervical carcinoma by CBCT of TrueBeam Linear accelerator.Methods 30 cases of postoperative cervical cancer patients were randomly divided into two groups,group A with popliteal fossa fixed method by trapezoidal fixation,group B with traditional vacuum pad fixation.CBCT was used to record both setup errors and rotational errors,Stroom extension formula was used to calculate the PTV expansion value coming from the two different fixation methods.Results There was significant difference in setup errors between group A and group B.The setup errors in the left-right direction (X),cranial-caudal direction (Y) and anterior-posterior direction (Z) were (0.19±0.14) cm,(0.17±0.12) cm and (0.13±0.11) cm in group A,respectively.On the contrary,the setup errors in X,Y and Z were (0.24±0.19) cm,(0.25±0.21) cm and (0.22±0.18) cm in group B,respectively.The rotational errors were 0.05°±0.02° in group A,comparing with 0.5°±0.21° in group B (P =0.00).The PTV expanded margin in group A was 0.56 cm in X direction,0.51 cm in Y direction,0.40 cm in Z direction,in comparing with 0.73 cm,0.78 cm and 0.67 cm in group B,respectively.Group A remarkably reduced the PTV,pelvis,small intestine,bladder and rectum irradiated volumes [(1 167±271) mm3 vs (1 379±297) mm3,(84±12) mm3 vs (130±17) mm3,(81±51) mm3 vs (117±64)mm3,(62±40) mm3 vs (75±47) mm3,(21±16) mm3 vs (31±21) mm3].Conclusion Popliteal fossa fixed method can reduce setup errors and improve the stability of positioning,more suitable in precise radiotherapy for postoperative cervical cancer patients,which has the value of further validation.

Objective To study the feasibility of laparoscopic cholecystectomy in low pneumoperitoneum pres sure and the effct of low pressure pneumoperitoneum on liver enzymes.Methods A total of 128 cases of laparoscopic gallbladder resection for gallbladder stones or chronic acalculous cholecystitis patients came from outpatient of Department of Two General Surgery of Daxing Hospital Affiliated to Capital Medical University from Jan.2010 to Apr.2015.Patients with odd hospital numbers were divided into the conventional pressure group (group A:12-14 mmHg,n =64).Patients with even numbers were divided into the low pressure group (group B:8-10 mmHg,n =64).The venous blood was respectively collected at the first day and second day preoperatively to determine and compare the contents of alanine aminotransferase (ALT) and aspartate aminotransferase(AST) in two groups.The mean and standard deviation of the above indexes and operation time of two group patients are compared.Results All patients were successfully completed surgery.The average operation time of group A was (65 ± 6.5) minutes,and group B was (61 ± 8.2) minutes,and there was no significant difference between the two groups (t =2.212,P =0.976).Compared with the A group,the B group had less effect on the aminotransferase,the data of the two groups were significantly different (P ＜ 0.05).Conclusion Laparoscopic gallbladder resection underwent the low pneumoperitoneal pressure (8-10 mmHg) is safe and feasible,doesnt extend the operation time,and helps to reduce the effect of pneumoperitoneum pressure on transaminase,which is much important for patients with abnormal aminotransferase preoperative.

The laboratory teaching of bacteriological analysis is very important for training practical analysis professionals.For better quality of laboratory teaching,in this paper,we practice a series of reform facilitating the lab teaching of bacteriological analysis,which is involved in the exploration and study of new curriculum system and teaching evaluation.The educational practices create effective teaching and learning environment and better performance.

Objective To investigate effect of specialized preoperative visit for patients in special operation position.Methods A total of 80 patients in special operation position (thyroid surgery for example) were randomly divided into observation group and control group,the control group was given routine operative interview,and the observation group was given specialized preoperative visit,and postural training,position tolerance,operation comfort and visit satisfaction of two groups were compared.Results The exercise enthusiasm of body position and the postural fitness in the observation group were higher than that in the control group,the difference was statistically significant (P ＜ 0.05).The headache and dizziness,nausea and vomiting in the observation group were less than that in the control group,the difference was statistically significant (P ＜ 0.05).The visit satisfaction in the observation group was higher than that in the control group,the difference was statistically significant(P ＜ 0.05).Conclusion Specialized preoperative visit can improve the adaptability of the body position,reduce the postoperative adverse reaction,and improve the satisfaction of visiting.

Objective To investigate effect of specialized preoperative visit for patients in special operation position.Methods A total of 80 patients in special operation position (thyroid surgery for example) were randomly divided into observation group and control group,the control group was given routine operative interview,and the observation group was given specialized preoperative visit,and postural training,position tolerance,operation comfort and visit satisfaction of two groups were compared.Results The exercise enthusiasm of body position and the postural fitness in the observation group were higher than that in the control group,the difference was statistically significant (P ＜ 0.05).The headache and dizziness,nausea and vomiting in the observation group were less than that in the control group,the difference was statistically significant (P ＜ 0.05).The visit satisfaction in the observation group was higher than that in the control group,the difference was statistically significant(P ＜ 0.05).Conclusion Specialized preoperative visit can improve the adaptability of the body position,reduce the postoperative adverse reaction,and improve the satisfaction of visiting.

Objective To compare the effects of two kinds of lidocaine gel indwelling catheter on male patients with general anesthesia during recovery period. Methods A total of 90 cases of male patients with general anesthesia were selected and divided into experimental group and control group by random digits table method,45 cases in each group. The experimental group was given lidocaine gel injection indwelling catheter urethral surface anesthesia, the control group will lidocaine gel evenly to the catheter indwelling catheter. The incidence of catheter related bladder irritation and restlessness were observed during the recovery period of general anesthesia. Results The incidence of catheter related bladder irritation in grade 0, I, II, III during the recovery period of general anesthesia were 28, 14, 3, 0 cases in the experimental group and 7, 23, 12, 3 cases in the control group, there was significant difference between 2 groups (Z=1500, P<0.01). The incidence of restlessness in grade 0, I, II and III during the recovery period of general anesthesia were 27, 18, 0 and 0 cases, respectively in the experimental group and 7, 20, 9, and 9 cases in the control group, respectively. The difference between the 2 groups was statistically significant (Z=1435.5, P<0.01). Conclusions Lidocaine gel injection into the urethra surface anesthesia can effectively improve the comfort of male patients during the recovery period of general anesthesia.

OBJECTIVETo introduce the technique of intrathoracic surgery performed through vertical transaxillary minithoracotomy.

METHODSFrom March 1989 to March 2001, 316 patients underwent intrathoracic surgery through a vertical transaxillary minithoracotomy. 285 patients suffered from patent ductus arteriosus (PDA), 10 congenital esophageal atresia, 8 congenital pulmonary cysts, 6 congenital emphysema, 1 pulmonary sequestration, 5 mediastinal tumor, and 1 eventration of the diaphragm.

RESULTSAll of the patients were successfully treated under satisfactory exposure. No operative mortality and severe postoperative complications occurred.

CONCLUSIONSIntrathoracic surgery performed through a vertical transaxillary minithoracotomy appears to be less invasive, and is a simple, safe, cosmetically acceptable and efficient approach.

Adolescent ; Axilla ; Child ; Child, Preschool ; Cysts ; congenital ; surgery ; Ductus Arteriosus, Patent ; surgery ; Esophageal Atresia ; surgery ; Female ; Humans ; Infant ; Infant, Newborn ; Lung Diseases ; congenital ; surgery ; Male ; Pulmonary Emphysema ; congenital ; surgery ; Thoracotomy ; methods