Objective To analyze the diagnosis and treatment of bone hydatid disease retrospectively. Methods From October 1957 to February 2004, thirty-seven consecutive cases, which were 16 males and 21 females, underwent debridement operation. The average age was 29 years ranging from 14 to 58 years. The history of bone hydatid disease was 3.1 years ranging from 0.5 to 12 years. The lesion was located at cervical vetebrae in 2, scapula in 1, thoracic vetebrae in 11, rib in 2, lumbar vetebrae in 5, ilium in 1, sacrum in 1, pelvic pubis in 1, hip joint in 2, femoral intertrochanter in 1, proximal humerus in 2, proximal tibia in 1, humeral head in 1, and proximal rudius in 1. The lesions of all cases were performed curettage thoroughly accepted and some of them received autogenetic or allogenetic bone graft, and artificial bone or bone cement was used to fill the defect in a few cases. Albendazole was used to prevent relapse for 3 months after operation, the dose of Albendazole tablets or powder was 20 mg/kg per day, or liposomal Albendazole 10 mg/kg per day. Results 24 cases were followed up; the period was 2 to 20 years with an average of 3.6 years. Of 37 cases, 31 were hydatid disease of trunk bone (83.78%), 24 were spinal hydatid disease. 25 of 37 cases were performed Casoni test, 21 cases were positive(84%). Four cases accepted the 8-tests immunodiagnosis for human hydatidosis, all were positive. MRI examination was taken in 21 of 37 cases, 18 cases were diagnosed as bone hydatid disease. In 24 cases which were followed up, 11 cases relapsed(45.83%). Conclusion Bone hydatid disease often occurs in the bone of trunk, especially in spine; the X-ray or CT images of bone hydatid disease are similar to tuberculosis, metastases, giant cell tumor, or cyst of bone, it should be identified with these diseases; MRI is valuable to diagnosis of spinal hydatid disease; serological examinations are the major method of identification diagnosis; spinal hydatid disease can not be eliminated easily by operation, and often relapses.

Objective To evaluate the efficacy of Jianpi-Xiaozheng recipe combined with chemotherapy in patients with late gastric cancer. Methods A total of 124 patients with late gastric cancer were randomly divided into a control group and a treatment group by random number table method, with 62 cases in each group. The patients in the control group received chemotherapy with S-1 and oxaliplatin (SOX), and those in the treatment group received Jianpi-Xiaozheng recipe combined with SOX chemotherapy. The treatment response was evaluated using the response evaluation criteria in solid tumors. The quality of life and physical status were evaluated with the European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire (QLQ-C30) and Karnofsky Performance Status (KPS), respectivly. The serum levels of tumor biomarkers, carbohydrate antigen 199 (CA199) and carbohydrate antigen 72-4 (CA72-4) were detected before and after treatment. Results The response rate (complete or partial response) in the treatment group was significantly higher than that in the control group (62.9%vs. 43.5%;χ2=4.665, P=0.031). The scores of QLQ-C30 (46.8 ± 6.3 vs. 42.2 ± 5.9;t=4.196, P=0.001) and KPS (79.1 ± 7.8 vs. 72.0 ± 7.5;t=5.167, P=0.000) in the treatment group were significantly higher than those in the control group. The serum levels of CA199 (61.7 ± 16.5 U/ml vs. 113.3 ± 21.4 U/ml;t=15.036, P=0.000) and CA72-4 (27.9 ± 9.6 U/ml vs. 34.3 ± 9.7 U/ml;t=3.693, P=0.001) in the treatment group were significantly lower than those in the control group. Conclusions Jianpi-Xiaozheng recipe combined with chemotherapy can increase response rate, decrease the serum levels of tumor biomarkers, and improve the quality of life in patients with late gastric cancer.

Objective:To establish a finite element model for bone transport before surgery in the treatment of femoral and tibial bone defects with external fixation and combined intramedullary nails with external fixations, and evaluate the stability and properties of biomechanics.Methods:Between May 2022 and August 2022, a male volunteer in the Department of Trauma and Microreconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University was selected. The right lower limb was scanned using 64-slice CT. Data were imported into Mimics 21.0 to establish normal geometric models of femur and tibia. The models were arranged in 4 groups: a femoral external fixator group, a femoral external fixator combined with intramedullary nail group, a tibial external fixator group and a tibial external fixator combined with intramedullary nail group. Hypermesh 10.0 was used for meshing. Finite element analysis was performed by Ansys v.11 to measure the distribution and characteristics of equivalent stress in the transported bone segment, proximal and distal tibia, and fixtures in the 2 treatment modalities, respectively.Results:Peak Von Mises equivalent stress of cortical bone and external fixation was found higher in both of the femoral and tibial external fixator combined with intramedullary nail groups than that in the external fixator group, with stress reduction on cortical bone at approximately 76.9% and 77.8%, respectively. The stress reduction on external fixator was about 81.4% and 76.3%, respectively. Peak displacement of the structure in both of the femoral and tibial external fixator combined with intramedullary nail groups was lower than that in the external fixator group, with 78.4% and 60.1% reduction in displacement, respectively.Conclusion:Bone transport with intramedullary nailing combined with external fixator in treatment of femoral and tibial defects would offer better biomechanical advantages. It can facilitates bone regeneration and bone mineralisation during distraction phase and consolidation phase.

BACKGROUND:Filamin B(FLNB)can crosslink the actin cytoskeleton into a dynamic structure that is essential for the directional movement of cells.It can regulate the proliferation,differentiation and apoptosis of chondrocytes.However,the effect of FLNB on osteoblast proliferation,migration and apoptosis has not been reported. OBJECTIVE:To investigate the effect of FLNB on the proliferation,migration and apoptosis of MC3T3-E1 cells. METHODS:The adenoviral vectors for knockdown of FLNB expression(sh-FLNB1,sh-FLNB2,sh-FLNB3)were constructed and infected with MC3T3-E1 cells.After screened by puromycin drug,the efficiency of FLNB knockdown was detected by western blot and RT-PCR.The MC3T3-E1 cell line with the best efficiency of FLNB knockdown was selected as the stable transient cell line of MC3T3-E1 for subsequent experiments.The cells were divided into blank group,mc3t3 group,sh-NC group(empty vector),and sh-FLNB group(sh-FLNB lentivirus).The blank group was cultured in cell-free α-MEM complete medium;the mc3t3 group was cultured in α-MEM complete medium alone;and the sh-NC and sh-FLNB groups were cultured with α-MEM medium containing 2.5 μg/mL puromycin.After 3 days of culture,cell counting kit-8 assay and cell scratch assay were used to detect the proliferation and migration ability of MC3T3-E1;flow cytometry was used to detect cell apoptosis;and RT-PCR was used to detect the expression of apoptosis-related genes. RESULTS AND CONCLUSION:Western blot and RT-PCR results showed that the efficiency of FLNB knockdown was the best in the sh-FLNB3(P<0.000 1),which was used as a stable cell line for subsequent experiments.Cell counting kit-8 data showed that the proliferative ability of MC3T3 cells was significantly weakened after knockdown of FLNB(P<0.05).Cell scratch assay results showed that the migration ability of MC3T3 cells was significantly decreased after knockdown of FLNB.Flow cytometry and RT-PCR results showed that the apoptotic rate of MC3T3-E1 cells increased after knockdown of FLNB,the expression of pro-apoptotic factor Bax increased significantly,and the expression of anti-apoptotic factor Bcl-2 decreased significantly(P<0.05).To conclude,knockdown of FLNB can reduce the proliferation ability of MC3T3-E1 cells,decrease the migration ability of the cells,and increase cell apoptosis.