Objective To investigate the clinical value of minimally invasive rotary resection in the treatment of mastitis with abscess formation. Methods A total of 93 cases with mastitis with abscess formation in our hospital from October 2010 to December 2014 were divided into two groups according to patients ’ willingness: one group was given ultrasound-guided Mammotome rotary surgery and drainage (minimally invasive group, n=45), the other underwent incision and drainage (control group, n=48).Surgical parameters, postoperative complications and patient satisfaction were compared between the two groups. Results The minimally invasive group was significantly superior to the control group in the operative time, blood loss, healing time, postoperative scar length and postoperative complications [(16.0 ±4.4) min vs.(30.6 ±6.8) min, t=-12.339, P=0.000;(8.5 ±2.3) ml vs.(12.7 ± 4.1) ml, t=-6.102, P=0.000;(10.1 ±1.9) d vs.(14.3 ±3.3) d, t=-7.595, P=0.000;(4.6 ±1.2) mm vs.(40.5 ± 11.8) mm, t=-20.913, P=0.000;8.9%(4/45) vs.25.0% (12/48), χ2 =4.232, P=0.040].Follow-up reviews at 3-6 postoperative months showed the comprehensive satisfaction of operation was significantly higher in the minimally invasive group (37/45, 82.2%) than that of control group (28/48, 58.3%) (χ2 =6.299, P=0.012). Conclusion For mastitis with abscess formation, minimally invasive rotary surgery has characteristics of minimal invasion, quick postoperative recovery, good cosmetic effects, few complications and high patients’ postoperative satisfaction.It deserves further clinical application.

Objective To investigate the effects of menaquinone-4(MK4)on the activation and function of CD8+T cells.Methods An in vitro culture system for primary mouse CD8+T cells was established by isolating these cells from the spleen using CD8a T cell isolation kit.The isolated CD8+T cells were then incubated and activated in a 96-well plate coated with anti-CD3/CD28 antibodies.The impact of MK4 on the activation and cytokine secretion of CD8+T cells was explored by quantifying the expression levels of CD8+T cell activation receptors and cytokines using flow cytometry.Additionally,the concentrations of these cytokines in the culture supernatant were measured by enzyme-linked immunosorbent assay(ELISA).The influence of MK4 on the anti-tumor function of CD8+T cells was evaluated by co-culturing colorectal cancer MC38 cells of mice with CD8+T cells at different ratios,and the effect of MK4 was assessed by detecting tumor cell apoptosis.Results High-purity primary CD8+T cells of mice(97.5％)were isolated using the magnetic bead,which could be activated by pre-coated CD3/CD28 antibodies and showed proliferation.Compared with the control group,the MK4-treated group exhibited increased expressions of CD25,CD69 and CD44 on CD8+T cells,as well as higher production and secretion levels of interleukin-2(IL-2),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)and granzyme B.In addition,CD8+T cells in the MK4-treated group induced a higher percentage of tumor cell apoptosis(36.7％)compared with the control group(29.1％).Conclusion MK4 can enhance the activation of CD8+T cells and promote anti-tumor functions.