1.EXPERIMENTAL STUDY OF COMBINATION TREATMENT WITH SODIUM MORRHUATE AND CIS-DIAMINOCHLOROPLATINUM FOR HUMAN GASTRIC CANCER
Peng HOU ; Zenghua LI ; Caifu XU ;
Chinese Journal of Digestive Endoscopy 1996;0(04):-
The antitumor effect of sodium morrhuate (SM ) and cis - diaminoaichloroplatinum (CDDP ) for gastric cancer cell SGC - 7901 in vitro is observed with MTT assay. Human gastric cancer cells line SGC - 7901 were inoculated subcutaneously in the naked mice , so xenografts were established. The tumor of the mice in the combination groups were cross injected with SM 50mg . kg~(-1) . time~(-1) , 50?l and CDDP ( 3mg ? kg~(-1)? time~(-), 50?l)in varying order at an interval of 30 minutes. In each mouse the tumors were injected every third davs for a total of three injections. lt was found SM had strong antitumor effect on gastric can- cer cells in vitro experiment. The antitumor effect of local injection in combination groups were much stronger than that in any other single drug groups. The histopathological section observation showed that all tumors cells were necrotic in combination groups, There could be seen fibrous histocysts around the . necrotic tumor tissue in observation groups , and necrotic vascular endotheliocytes. The experiment showed that combined injection with SM and CDDP had a synergic antitumor effect.
2.Clinical observation on laryngeal cough treated with acupuncture and moxibustion
Shurong YANG ; Zenghua XU ; Ying LI ; Xiaorui CHEN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(07):-
Objective:To observe the clinical efficacy of acupuncture combining with temperature sensitive point moxibustion therapy on laryngeal cough with yin-deficiency of lung-kidney. Methods:Divided 80 patients into test group and control group. Each included 40 people. The test group adopted the therapy by acupuncture kaiyin 1,Tianrong point,combining with temperature sensitive point (this point ofen appears in Yongquan Point Branch) moxibustion therapy,and the control group took the Western medicine conventional treatment. Then calculated the total effective rates of the two groups and analyzed statistically. Results:At the end of treatment,the total effective rate of test group was 95%,and the control group's was 85%,the difference between total effective rate was signifi cant difference (P
3.Expression of vascular endothelial growth factor in squamous cell carcinoma of oral cavity
Qingyuan ZHANG ; Jinrong LI ; Wuxiu LI ; Shuhua WU ; Zenghua LV ; Xiaoyun SUN
Journal of Practical Stomatology 1995;0(04):-
Objective: To study the role of vascular endothelial growth factor (VEGF) during angiogenesis in oral squamous cell carcinoma (SCC).Methods:Surgical specimens from 45 patients with oral SCC were examined for VEGF expression and microvessel density(MVD) by immunocytochemical staining. Anti-VEGF polyclonal antibody was used to determine VEGF expression and anti-CD 34 monoclonal antibody was used to determine MVD. Results:①VEGF protein was mainly found in cytoplasm of cancer cells and in few endothelial cells. Stained microvessels were mainly distributed in the regions of the connective tissue near the cancer nest;②there was a close positive correlation between MVD and VEGF expression;③SCC with high MVD or high VEGF level was mostly found with metastasis in cervical lymph node (P
4.Value of 99Tc m-MIBI SPECT/CT imaging in preoperative diagnosis of primary hyperparathyroidism and its influencing factors
Yingying ZHANG ; Na HAN ; Fengyu WU ; Jiao LI ; Chenghui LU ; Xinfeng LIU ; Guoqiang WANG ; Zenghua WANG ; Xufu WANG
Chinese Journal of Nuclear Medicine and Molecular Imaging 2021;41(6):345-349
Objective:To investigate the preoperative diagnostic value of 99Tc m-methoxyisobutylisonitrile (MIBI) planar imaging and SPECT/CT imaging for primary hyperparathyroidism (PHPT), and analyze the relevant factors affecting the imaging results. Methods:From June 2016 to September 2019, a total of 62 patients (15 males, 47 females, age range: 27-80 years) confirmed as PHPT by postsurgical pathology in Affiliated Hospital of Qingdao University were retrospectively enrolled. The diagnostic efficacies of 99Tc m-MIBI planar imaging and SPECT/CT imaging were compared using χ2 test. The differences of preoperative serum parathyroid hormone (PTH), Ca and the maximum diameter of lesion between the positive and negative groups of planar imaging were analyzed using independent-sample t test and Mann-Whitney U test. The region of interest (ROI) method was applied to calculate the uptake ratio of lesions to normal tissues at the early phase (T/Ne) and delayed phase (T/Nd) in positive cases of planar imaging. Pearson or Spearman correlation analysis was used to evaluate the correlation of T/Ne, T/Nd with preoperative serum PTH, Ca and the maximum diameter of lesion. The receiver operating characteristic (ROC) curves of preoperative serum PTH, Ca and positive planar imaging were drawn and the cut-off values were obtained. Results:The sensitivity of planar imaging and SPECT/CT imaging was 69.35%(43/62) and 87.10%(54/62) respectively ( χ2=5.729, P=0.017). The preoperative serum PTH, Ca levels and the maximum diameter of lesion in patients with positive planar imaging (253.32(107.00, 331.70) ng/L, 2.78(2.51, 2.87) mmol/L, (2.01±0.88) mm) were higher than those with negative planar imaging ((111.86±44.29) ng/L, (2.59±0.21) mmol/L, (1.42±0.55) mm; z values: -2.802, -1.978, t=3.300, all P<0.05). T/Ne was positively correlated with preoperative serum PTH ( rs=0.511, P<0.001) and the maximum diameter of lesion ( r=0.381, P=0.012), and T/Nd was positively correlated with preoperative serum PTH ( rs=0.538, P<0.001), Ca ( rs=0.348, P=0.022) and the maximum diameter of lesion ( r=0.463, P=0.002). The area under the ROC curve between preoperative serum PTH, Ca and planar imaging was 0.725 and 0.646, respectively. Preoperative serum PTH had a better predictive value with the optimal cut-off value of 150.4 ng/L. Conclusions:Preoperative serum PTH, Ca and the maximum diameter of lesion are positively correlated with 99Tc m-MIBI uptake in PHPT patients with positive planar imaging results. When preoperative serum PTH is lower than 150.4 ng/L, planar imaging is prone to false negative. SPECT/CT imaging has a significant value in preoperative diagnosis and the combination of PTH and CT can improve the positive rate.
5. Rapamycin affect the apoptosis of splenic CD4+CD25+ regulatory T cells of mouse severe aplastic anemia model
Zenghua LIN ; Hong LIU ; Li ZHU ; Xi YANG ; Yaping ZHANG ; Juan QIAN ; Haiyan LIU
Chinese Journal of Hematology 2018;39(3):196-201
Objective:
To explore the effects and possible mechanism of rapamycin (RAPA) on apoptosis of CD4+CD25+ Tregs from the mouse severe aplastic anemia (SAA) model.
Methods:
The BALB/c female SAA model mice were induced by interferon-gamma in combination with busulphan. The SAA model mice were intraperitoneal injection with RAPA at daily dose of 0.5 mg/kg for 5 days (the RAPA-treated group,
6. Treatment response of differentiated thyroid carcinoma with negative preablative stimulated thyroglobulin and iodine-positive lymph node after the first radioablation and influence factors analysis
Chenghui LU ; Jiao LI ; Xinfeng LIU ; Guoqiang WANG ; Zenghua WANG ; Xufu WANG
Chinese Journal of Nuclear Medicine and Molecular Imaging 2020;40(1):21-26
Objective:
To investigate the response to 131I therapy and to explore the influence factors in postoperative differentiated thyroid carcinoma (DTC) patients with negative preablative stimulated thyroglobulin (psTg) and iodine-positive lymph node after the first radioablation.
Methods:
From May 2016 to October 2018, 108 DTC patients (28 males, 80 females, age: (45.7±10.4) years) with negative psTg who underwent 131I treatment for the first time in the Affiliated Hospital of Qingdao University were retrospectively enrolled. All patients had iodine-positive lymph nodes, which were showed by SPECT/CT imaging 5-6 d after 131I treatment. The treatment response was evaluated at 6-24 month after 131I treatment. Patients were divided into excellent response (ER) group and non-excellent response (non-ER) group according to the response to the first 131I treatment. Independent-sample
7.Predictive value of cellular immune status before initial 131I treatment for treatment response in young and middle-aged patients with papillary thyroid cancer
Chenghui LU ; Xinfeng LIU ; Jiao LI ; Guoqiang WANG ; Zenghua WANG ; Na HAN ; Yingying ZHANG ; Xufu WANG ; Yansong LIN
Chinese Journal of Nuclear Medicine and Molecular Imaging 2023;43(2):102-105
Objective:To investigate the value of cellular immune status before initial 131I treatment for predicting treatment response in young and middle-aged patients with papillary thyroid cancer (PTC). Methods:From March 2018 to April 2019, 150 young and middle-aged patients with PTC (46 males, 104 females, age (40.0±9.8) years) who underwent total thyroidectomy and neck lymph node dissection in the Affiliated Hospital of Qingdao University were enrolled retrospectively. All patients underwent radioablation 1-2 months after operation, and the serum lymphocyte subsets (CD3 + , CD4 + , CD8 + , CD4/CD8) as well as natural killer (NK) cells were detected 1 d before the initial 131I treatment. Patients were divided into excellent response (ER) group and non-ER group according to the response of 6-12 months after 131I treatment. Clinicopathological characteristics, preablative stimulated thyroglobulin (psTg), initial 131I dose and lymphocyte subsets that might affect the response to 131I treatment were analyzed (independent-sample t test, Mann-Whitney U test, χ2 test, multiple logistic regression analysis). ROC curve analysis was used to evaluate the predictive value of significant factors for non-ER. Results:Of 150 patients, 84 cases were in ER group (56.00%), and 66 cases (44.00%) were in non-ER group. Age ( z=-2.86, P=0.004), M stage ( χ2=13.64, P<0.001), psTg ( z=-8.94, P<0.001), initial 131I dose ( z=-7.60, P<0.001), CD4 + ( t=2.50, P=0.014), CD4/CD8 ( z=-2.22, P=0.027) of the two groups were significantly different. Multivariate analysis showed that psTg (odds ratio ( OR)=1.27, 95% CI: 1.16-1.40, P<0.001) and CD4/CD8 ( OR=0.39, 95% CI: 0.15-0.99, P=0.048) were independent factors for predicting 131I treatment response. The cut-off values of psTg and CD4/CD8 for predicting non-ER were 6.78 μg/L and 1.67, respectively. Conclusions:Cellular immune status before initial 131I treatment may predict treatment response in young and middle-aged patients with PTC. It indicates non-ER response when Tg is higher than 6.78 μg/L and CD4/CD8 is lower than 1.67.
8.Efficacy of ultrasound-guided bilateral erector spinae plane block for pediatric patients undergoing Nuss procedure with general anesthesia
Zenghua XU ; Jianmin ZHANG ; Chao ZHENG ; Jingjing CAI ; Tiehua ZHENG ; Lijing LI ; Fang WANG
Chinese Journal of Anesthesiology 2020;40(2):186-189
Objective:To evaluate the efficacy of ultrasound-guided bilateral erector spinae plane (ESP) block for pediatric patients undergoing Nuss procedure with general anesthesia.Methods:Thirty-two American Society of Anesthesiologists physical status Ⅰ or Ⅱ patients, aged 4-15 yr, undergoing Nuss procedure with general anesthesia, were divided into ESP block plus general anesthesia group (group EG, n=16) and general anesthesia group (group G, n=16) using a random number table method.Bilateral ESP blocks were performed after anesthesia induction in group EG.Intravenous analgesia was performed postoperatively in both groups.Face Legs Activity Cry Consolability (FLACC) scores both at rest and during coughing were recorded on admission to postanesthesia care unit (PACU) and at 1, 6, 12, 24 and 48 h after operation.When FLACC score at rest >4, ibuprofen 5.0-7.5 mg/kg was taken orally according to the requirements of pediatric patients and their parents.The intraoperative amount of propofol and remifentanil used and postoperative requirement for fentanyl and ibuprofen were recorded.The development of postoperative hypoxia (SpO 2 <90%), nausea and vomiting, and urinary retention was recorded.The development of puncture site infection, hematoma and subcutaneous emphysema was recorded.The tracheal extubation time, duration in PACU and length of postoperative hospital stay were also recorded. Results:Compared with group G, FLACC scores at rest within 12 h after operation and during coughing within 6 h after operation were significantly decreased, the intraoperative consumption of remifentanil was reduced, the postoperative requirement for fentanyl and ibuprofen was decreased, the incidence of hypoxemia was decreased, the tracheal extubation time and duration in PACU were shortened ( P<0.05), and no significant change was found in the consumption of propofol, incidence of nausea and vomiting, or length of postoperative hospital stay in group EG ( P>0.05). Conclusion:Ultrasound-guided bilateral ESP block can reduce the consumption of opioids in the perioperative period and enhance the analgesic efficacy with a higher safety, which is helpful in promoting short-term outcomes for pediatric patients undergoing Nuss procedure with general anesthesia.
9.Anesthesia management of children undergoing resection of pheochromocytoma and paraganglioma
Zenghua XU ; Jianmin ZHANG ; Nan ZOU ; Tiehua ZHENG ; Lianghong HUO ; Lijing LI ; Fang WANG
Chinese Journal of Anesthesiology 2024;44(2):209-213
Objective:To retrospectively analyze the anesthetic management characteristics of children undergoing resection of pheochromocytoma and paraganglioma (PPGL).Methods:The clinical data from patients undergoing resection of PPGL and confirmed histologically from January 1, 2010 to June 30, 2023 were retrospectively collected. The baseline characteristics, intraoperative conditions and postoperative complications were recorded.Results:The clinical data from 47 pediatric patients were analyzed. The overall incidence of hemodynamic instability events was 68% (32 cases). Lowering preoperative blood pressure to normal levels and the maximum diameter of tumor≥6 cm was helpful in reducing the incidence of the intraoperative hemodynamic instability events ( P<0.05). Postoperative hypotension developed in 7 cases, acute left heart failure in 1 case, arrhythmia in 1 case, adrenocortical insufficiency in 4 cases, and pulmonary infection in 13 cases. Conclusions:Thorough preoperative preparation, evidence-based anesthetic management, and meticulous postoperative vital sign monitoring can increase the perioperative safety for children undergoing resection of PPGL, thereby reducing the incidence of complications.