Objective: To sum up experiences and lessons about management of soft-tissue reconstruction in open tibial fracture over a 6-year period.Methods: Twenty-two flap reconstructions were performed to treat soft-tissue defect of 22 patients with open tibial fracture Type IIIB (Gustilo) from 1993 to 1998. The cases were analyzed and discussed retrospectively after follow up of 12-61 months.Results: The size of the flap ranged from 6.6 cm2 to 28.18 cm2 and the rate of flap failure was 13.6％. Besides, 3 partial necrosis and 2 postoperative infections occurred in this series.Conclusions: For soft tissuedefect of delayed open tibial fracture Type HIB, flap reconstruction is still an optimal option. The experiences we obtained are ① to design a triangular skin extension or a small Z-plasty over the pedicle to reduce the flap tension; ② to select a unilateral external fixation to provide convenience for anysecondary manipulation; and ③ to use serial debridementto diminish flap failure.

Aim To investigate the effect of magnetic nanoparticleencapsulated epirubicin(MNPE) on inducing apoptosis of human liverHep-6 tumor cell line in vitro and provide new method for local ablation ofliver in order to improve survival period of patients and quality oflife. Methods Inductive apoptosis of nano-magnetic pharmoparticle toHep-6 tumor cell of primonary hepatic cell caner was investigated by DNAelectrophoresis, electron nicroscopy , and flow cytometry analysis. Theseitems were divided into three groups, control, drug-control, and grouptreated with magnetic nauoparticle encapsulated epirubicin. The changes ofhuman liver Hep-6 apoptosis induced by magnetic nanoparticle encapsu-lated epirubicin were observed on different time-point and with differentnanoparticle encapsulated epirubicin and control group of biaoroubixingwere divided into high-dosage and low-dosage group. And the ultimateconcentration of 10 mg/L and 100 mg/L were given respectively on hu-group was iucreased from 25% to 54% after 24 hours. The apoptosis ratein the experimental group, biaoroubixing group and control group was78%, 53% and 2% respectively after 36 hours. There was significantdifference( t = 3.05. P ＜ 0.05) between the results of each group. Theapoptosis rate and quantity of medicine presented positive relativity withtime ( r = 0.96, P ＜ 0.05 ) .Conclusion Magnetic nanopartiele encap-sulated epirubicin presents the advantages of slow degradation, release ofmagnetic nanoparticle system and better target and can induce apoptosis ofliver tumor Hep cell.