Cluster Analysis ; Fuzzy Logic ; Humans ; Mathematics ; Medicine, Chinese Traditional

Objective:To analysis the clinical characteristics of pulmonary hypertension (PH) complicating pregnancy, then to explore the delivery time and the monitodng as well as the treatment during perinatal peri-od.Methods:The data of 10 cases with PH treated in our hospital from June 2005 to January 2009 were retro-spectively analyzed.Reaults:1 case was primary pulmonary hypertension(PPH) ; 9 cases were secondary PH (SPH), in which 4 cases were rheumatic heart disease and 5 cases were congenital heart diseases.3 cases were slight PH,6 cases were moderate PH, and 1 were severe group.4 cases NYHA class were Ⅰ～Ⅱ and 6 cases NYHA class were Ⅲ～Ⅳ.4 cases were term delivery,5 cases were premature delivery, and 1 got artifi-cial abortion.8 cases were cesarean section, and 1 was vaginal delivery.2 cases were very low birth weight infants, 1 infant was death and 1 mother was death.Conclusions:The patient of PPH should avoid concei-ving.For SPH patients whether to conceive depends on cardiac function, the level of pulmonary arterial pres-sure and should better get pregnancy after surgery or medication.It should terminate pregnancy in the first tri-mester for moderate or severe patients.Cesarean section is more suitable for those in the second or third tri-mester.The prognosis of the mother and neonate depends on several factors.

Objective To observe the effect of triplex surgical treatment, including rectal mucosa ligation,Xiaozhiling injection,encircing fighting surgery of the anus ring,on complete rectum prolapse. Methods From February 2007 to February 2010,fifteen cases with complete rectum prolapse were enrolled into this study and accepted triplex surgical treatment All patients were followed up for 1-4 years. Results The curative rate of the triplex surgical treatment was 100% with no serious complications. Conclusion Triplex surgical treatment was a simple, effective and reliable method in treating complete rectum prolapse, with less pain,fewer complications and quicker recovery.

Epigenetics, which is called second genetic code precisely and controls the total metabolic processes of eukaryotic cells,has an inseparable correlation with human health and diseases. Among all the gene silencing mechanisms of epigenetic modifications,CpG islands of DNA promoter hypermethylation/histone mod-ifications are two major causes. Promoter methylation of specific genes,such as Ras protein activator analogue 1 may lead to aberrant activation of downstream signaling pathways,following by fibroblasts proliferation and kid-ney fibrosis. In recent years,epigenetic modifications have been proved to play a major role in preventing fibro-blasts to return to their quiescent stage,ultimately contributing to fibrosis in the kidney. This paper provides a glimpse of recent studies on epigenetic mechanisms of chronic kidney diseases,especially renal fibrosis,with an aim to provide new insights into pathogenesis research and clinical diagnosis and treatment.

Objective To study the differences of CXCR7 expression between in osteosarcoma tissues and normal peri-osteosarcoma tissues, and observe the effect on cell proliferation and inflammatory cytokine PGE2 secretion in MG63 cells when it had been transfected with pEGFP-Adv-CXCR7 vector. Methods CXCR7 expression in osteosarcoma tissues, cells and normal peri-osteosarcoma tissues detected by RT-qPCR. The CXCR7 mRNA expression, cell proliferation and PGE2 secretion of MG63 cells after transfected with pEGFP-Adv-CXCR7 expression vector were evaluated by RT-qPCR, MTT and ELISA, respectively. Results The expression rate and quality of CXCR7 mRNA in osteosarcoma tissue were 93.80% and (2. 221 ±0. 112), which significantly higher than those of normal peri-osteosarcoma tissues 45.71% and (0. 3448 ±0.098). Low expression of CXCR7 in MG63 cells was also observed. The proliferation and PGE2 secretion of MG63 cells were significantly increased at the time point of 48, 72 and 96h after pEGFPAdv-CXCR7 expression vector transfected into MG63 cells ( P < 0. 05). Conclusion The expression of CXCR7 mRNA in osteosarcoma tissues were significantly higher than normal peri-osteosarcoma tissues. CXCR7 high expression may have a negative effect on the progress of osteosarcoma.

Angiogenesis is an important self-repair and remodeling mechanism after cerebral ischemia. It plays pivotal roles in promoting local blood circulation, protecting neurons and improving neurological function after cerebral ischemia. A variety of molecules and signal transduction pathways involved in the regulation of the process, including angiogenin, growth factors, matrix metalloproteinases, tissue kallikrein and anti-angiogenic proteins. This article reviews the roles of these molecules and signal transduction pathways in angiogenesis after cerebral ischemia.

Objective To explore the effects of Ganoderma spore(germination activated Ganoderma spore) on survival and expression of neurotrophin_3(NT_3) and nitric oxide synthase(NOS) of injured motor neurons in rat spinal cord. Methods Different dosages of Gandoerma spore were irrigated into the rat's stomach respectively in experimental groups after oneside ventral root cut.The survival ratio of injured motor neurons was counted.NT_3 expression of surviving motor neurons was measured by immunohistochemistry and in situ hybridization methods and NOS activity was measured by enzymo_histochemistry method. Results Thirty_five days after ventral root cut,the survival ratio of motor neurons was 47.32% in control group,and those were 67.11%,72.67% and 81.67% respectively in low,medial and high dosage Ganoderma spore groups.Expressions of NT_3 and NOS of surviving motor neurons in high dosage Ganoderma spore group were higher than those in control group.Conclusion Ganoderma spore may promote the survival of injured spinal motor neurons,and survival ratio of injured motor neurons is related to the dosages of Ganoderma spore.Ganoderma spore may also enhance the expressions of NT_3 and NOS of surviving spinal motor neurons.

Objective Secondary metabolites of the soft coral were studied to find out the lead compound of the drugs. Methods Compounds from the soft coral Sinularia papillosa were obtained by column chromatography and modern isolation methods and their structures were identified by spectrum of UV,IR,NMR,MS,et al. Results Two white amorphous compounds were obtained from the EtOAc (ethyl acetate) fraction,and their structures were identified as pregn-5,20 -dien-3?-ol-3-O-?-L-fucopyranoside (1) and 1-(17Z-tetracose-nyloxy)-1,2 -propanediol (2) by spectral analysis. Conclusion Two compounds are first isolated from the soft coral genus Sinularia,which will be beneficial for the taxonomy of the soft coral and for biogenesis of steroids.

Objective:Correlated factors of CET-4 scores and its washback effect on college English teaching were studied.Methods: more or less 1000 medical students′ CET-4 scores and their CET test anxiety levels were analyzed with SPSS 13.0 and Excel software.Result: It was shown that medical students′ CET-4 scores are negatively correlated with their CET-4 test anxiety levels,and positively correlated with the English scores in College Entrance Examination with significance,also correlated with their genders,their graduating senior high schools,their parents′ educational background,and without correlation with their majors.Conclusion: The positive effect of CET-4 washback can be applied to improve college English teaching quality and the aptitude of English language learning among medical college students.