Humans ; Molecular Diagnostic Techniques ; Neoplasms ; pathology ; Pathology, Molecular ; methods ; trends ; Pathology, Surgical ; methods ; trends ; Translational Medical Research

Diagnosis, Differential ; Humans ; Neoplasms ; classification ; genetics ; pathology ; Survival Analysis ; Terminology as Topic ; World Health Organization

Adenocarcinoma ; classification ; genetics ; pathology ; Adenocarcinoma, Bronchiolo-Alveolar ; genetics ; pathology ; Exons ; Humans ; Lung Neoplasms ; classification ; genetics ; pathology ; Mutation ; Oncogene Proteins, Fusion ; genetics ; Proto-Oncogene Proteins ; genetics ; Proto-Oncogene Proteins p21(ras) ; Receptor, Epidermal Growth Factor ; genetics ; ras Proteins ; genetics

Antibodies, Monoclonal ; administration & dosage ; Antigens, CD20 ; drug effects ; metabolism ; Antineoplastic Agents ; administration & dosage ; Drug Delivery Systems ; methods ; Fusion Proteins, bcr-abl ; drug effects ; metabolism ; Genetic Therapy ; Humans ; Neoplasms ; metabolism ; pathology ; therapy ; Receptor, ErbB-2 ; drug effects ; metabolism

AIM To investigate the reativative and protective effect of Neferine (Nef) on the poisoning mice of cholinesterase (ChE) with dichlorves (DDVP) or dipterex (Dip) of LD 100 . METHODS The mice were randomly divided into poisoning group with DDVP or Dip and Nef, Atropine and Pyraloxime methylchloride (PAM Cl) treatment group. Activity of mice blood ChE was determined with Ellmaris method, and acute toxicity experiment was used to determine the protective effect. RESULTS ① Nef (15 mg?kg -1 , ip) exhibited a definite protective effect on poisoning mice, and the mortality of the animals treated with Nef was significantly lower than that of the control group with normal saline ( P 0 05). But Nef and atropine group was significantly lower than that of thePAM Cl group ( P

Objective To explore whether ganoderma spores could help the neural epithelial cell of embryonic neural tube stagnated by retinoic acid in G-0/G-1 phase reenter the cell cycle,keep on proliferating and differentiating,and could reduce the occurrence of neural tube defects(NTDs). Methods When E7.75d,mice of the control group and the ganoderma spores group were given intragastrically onetime retinoic acid all-trans.Then the ganoderma spores solution was given intragastrically to the ganoderma spores group.At E10.5d,The embryos of the two groups were taken out and the ratio of NTDs was counted respectively Immunofluorescence histochemistry and flow cytometry were applied to examine the expression of nestin,and DNA quantity staining and RT-PCR to detect the mRNA transcriptions of Cdk2 and Cdk4. Results The radio of NTDs in the ganoderma spores group was lower than that in the control group remarkably,but the level of nestin was higher.With the neural tube cell cycles compared the cell radio of G-0/G-1 phase in the control group was higher than that in the ganoderma spores group,while the ratio in the S phase in the control group,it was lower than that of the normal embryos in the ganoderma spores group.The neural epithelial cell of embryonic neural tube in the ganoderma spores group could transcript Cdk4 mRNA normally,with a low transcription rate in the control group.Conclusion Ganoderma spores can reduce the occurrence of embryonic NTDs induced by retinoic acid in pregnant mice.

Military hospital human resources management departments is somewhat scattered, unfit to the hospital mission tasks and not conducive to the development of hospital.To integrate management department, to establish specialized human resources management departments is the new trend of military hospital human resources management development, and is also an important problem to be resolved.Change the military status of hospital human resources management, it is necessary to break through institutional constraints, use a development perspective and operating means, transform management and expand management thinking, so that to achieve the maximum effectiveness of management.

Adenocarcinoma, Papillary ; diagnosis ; metabolism ; Carcinoma, Pancreatic Ductal ; diagnosis ; metabolism ; Cystadenoma, Mucinous ; diagnosis ; metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Mucins ; classification ; genetics ; metabolism ; Pancreas ; metabolism ; Pancreatic Neoplasms ; diagnosis ; metabolism

Carcinoma, Neuroendocrine ; pathology ; Carcinoma, Squamous Cell ; pathology ; Humans ; Neoplasm Staging ; Thymoma ; classification ; pathology ; Thymus Neoplasms ; classification ; pathology ; World Health Organization

Objective To proceed deep data mining in large database obtained by Cancer Genome Anatomy Project and to distinguish the difference expression genes of gastric carcinoma.Methods The 300,783 serial analysis of gene expression records from 4 libraries of gastric cancer and 2 libraries of normal gastric tissue in database of the Cancer Genome Anatomy Project were analyzed by digital gene expression displayer.Results The 201 difference expression tags represented 136 genes(54 up-regulated and 82 down-regulated) and 65 ESTs(24 up-regulated and 41 down-regulated) were distinguished,and these genes were involved in biological process and signal transduction pathways,such as cell cycle regulation,cell proliferation and apoptosis.The gene expression profile in whole body normal and cancer tissues of PTMA were constructed by digital Northern based on serial analysis of gene expression database and by virtual Northern based on cDNA database,respectively.Conclusion The difference expression genes of gastric cancer can be distinguished effectively with bioinformatics,and the results will guide our further molecular biology research.If validated by molecular biology experiment,the difference expression genes will be used as molecular targets of gastric carcinoma.