The roles of information section of medical information construction are very important. By combining with the information working experience of hospital,the functions of information section in medical information construction are introduced from five aspects,including the role of decision-making staff,the role of organization and coordination,the role of management support,leading role and the role of information service,which can offer the experience for information construction of the other hospitals.

Cytochrome P450 are abundant in Streptomyces which play an important role in the biosynthesis of secondary products and metabolism of exotic chemicals of Streptomyces. Recent progress and function of cytochrome P450 in Streptomyces were reviewed in this paper. The problems in study of Streptomyces Cytochrome P450, and the prospects for future study of cytochrome P450 and its application were also discussed.

Objective To investigate the glial activation in the spinal cord in a rat model of persistent postoperative pain. Methods Forty-eight adult male SD rats weighing 200-250 g were randomly divided into 2 groups ( n = 24 each): group Ⅰ sham operation (group S) and group Ⅱ persistent postoperative pain. Persistent postoperative pain was evoked by skin/muscle incision and retraction (SMIR) as described by Flatters. Pawwithdrawal threshold to yon Frey hair stimulation was measured before operation (baseline) and at 1, 3, 12, 22and 32 d after establishment of the model. Four animals were sacrificed at each time point and lumbar segment of the spinal cord was removed for determination of expression of glial fibrillary acidic protein (GFAP) in the astrocytes by immunofluorescence histo-chemistry assay. Results The mechanical threshold started to decrease at 1 d after establishment of the model, and peaked at 12 d after establishment of the mode. Immunofluorescence histochemistry assay demonstrated that GFAP expression in the dorsal horn was significantly increased at 3 d after estabhshment of the model and reached the peak at 12 d and was maintained at the high level until 22 d after establishment of the model. Conclusion Glial activation is involved in the mechanism of persistent postoperative pain evoked by SMIR.

Objective To investigate the relationship between apoptosis of peripheral blood lymphocytes (PBLCs) and impaired immunity by assessing the apoptosis and expression of Fas and Bcl-2 in PBLCs of patients with secondary syphilis. Methods Flow cytometry (FCM) was used to detect PBLC apoptosis as well as Fas and Bcl-2 expression in 33 patients with secondary syphilis and 30 normal controls. Results The Fas expression on PBLCs and CD4+ cells was significantly higher in patients than that in controls (P 0.05). The apoptosis levels were significantly higher in PBLCs and CD4+ cells in patients than those in controls (P 0.05). In patients group, the apoptosis levels of both PBLCs and CD4+ cells had a positive correlation with the Fas expression (r = 0.68, P

A 13-year-old girl suddenly developed hypertrophic rough-surfaced plaques on both elbows, knees and ankles without obvious precipitating factors at 3 years of age.Because there was no subjective symptom or other discomfort, no treatment was given.Thereafter, the lesions neither faded nor spread.Skin examination revealed lesions covered with 1-2 mm-sized keratotic papules on the elbows and knees, which were clustered together and confluent in some areas.Circular keratotic plaques were observed on the ankles.Histopathology showed epidermal hyperkeratosis with mild parakeratosis, follicular keratotic plugs, acanthosis, broadened dermal papillae, telangiectasis and perivascular mononuclear infiltration in dermal papillae, infiltration of a few nonspecific cells in the superficial dermis.According to the clinical manifestations and histopathological findings, the patient was diagnosed with keratosis circumscripta, and treated with oral vitamin A and topical halometasone cream and urea-vitanmin E cream.The degree of skin hypertrophy and roughness was decreased after 15 days of treatment, but increased 1 month after drug withdrawal.

ObjectiveTo assess the effects of dopamine,dobutamine and norepinephrine on the P(g-a)CO2 and superior mesenteric blood flow in septic shock.MethodsRabbit septic shock model was established by challenging with intravenous injection of lipopolysaccharides from Escherichia coil(2 mg/kg).The rabbits with septic shock were randomly assigned to 3 groups-dopamine group(n = 8),dobutamine group(n = 8) and norepinephrine group(n = 8).Apart from volume resuscitation with normal saline solution [20 ml/(kg· h)]，dopamine[5μg/(kg·min)],dobutamine[(5μg/(kg·min)]and norepinephrine [(1μg/(kg·min)]were infused in dopamine group,dobutamine group and norepinephrine group respeclively.Cardiac index(CI) and superior mesenteric blood flow index(SMBFI) were continuously monitored by doppler flowrneter.Gastric mucosal PCO2 was evaluated by gas tonometry every 10 min.Arterial and venous blood gas analyses and lactate levels were measured every 1 h.ResultsMAP,CI,and SMBFI significandy decreased and P(g-a) CO2 increased after lipopolysaccharides infusion in three groups.After 2-hour treatment,MAP in norepinephrine group[(70 +3) mm Hg]was higher than that of dopamine group[(66±4) mm Hg]and dobutamine group[(65±4) mm Hg](P ＜0.05).SMBFI in norepinephrine group [(18.7±2.9) ml/(kg·min)]was higher than that of dopamine group[(16.2±1.6) ml/(kg·min)]and dobutamine group[(15.8±1.9) ml/(kg·min)](P＜0.05).P(g-a) CO2 in norepinephrine group [(30±6) mm Hg]was lower than that of dobutamine group[(23±5)mm Hg](P＜0.05).Condnsion As an adjuvant therapy of volume resuscitation,norepinephrine is more effective than low dose dopamine and dobutamine in improving splanchnic perfusion.

Objective: This paper aims to examine the current situation of information integration platform construction in tertiary hospitals in China, and explore the existing problems in the development of the integrated platform.Methods: This paper studies the current situation of the development of the medical integration platform through literature, and the relevant data of the status quo of the information integration construction was obtained through the National Level 3 Hospital Information Interoperability Standardization Construction Survey Report (2015), and descriptively analyzed the hospital medical integration Platform construction of the specific circumstances.Conclusion: From this research, it is concluded that there is a lack of unified planning in the construction of the medical integration platform of the tertiary hospitals in China, which is post-repair.The data is difficult to share, the data security loopholes and hidden dangers still exist.Therefore, it will be very helpful to plan the standardization of hospital information integration platform and the construction of information security, deepening the integration of medical information on the lower level and other measures to solve the problem, this paper puts forward the overall plan for the hospital integration platform.

Objective To investigate HIV high risk population size estimation method,analyze the epidemic situation in China and offer scientific evidence for policy making. Methods Appropriate methods to estimate different type high risk populations were ascertained by experimental study. Results Size estimation methods and procedures for different type high risk population were formed.Epidemic estimation and parameters fit for China were ascertained.HIV epidemic situation and characteristics were stated. Conclusion Methods formed in the study are the key points for the understanding of number of PLWHA,for the exact judgment of HIV epidemic,for the improvement of inspection system and examination work,for the formation of "Four Free and One Care".

ObjectiveTo evaluate the effectiveness of surgical technique improvement in thyroid lobectomy for prevention of recurrent laryngeal nerve (RLN) injury. MethodsThe clinical data of 85 patients of thyroid disease undergoing 126 lobectomies from Jan 2010 to Jan 2011 were retrospectively analyzed.All patients undergone accurate thyroid lobectomy with accurate envelop anatomic dissection,skeletonized blood vessels. Recurrent laryngeal nerve was all dearly exposed.ResultsNo-bleeding surgical area was achieved.The RLN was exposed.Unilateral temporary RLN injury occurred in 2 cases.The rate of RLN injury was 1.59％(2/126). There was no bilateral and perpetual RLN injury.ConclusionsCarrying through the accurate thyroid lobectomy,the incidence of RLN injury is low.

Objective To investigate the effects of low-dose hydrocortisone on brain dysfunction in LPS-induced sepsis rats and the role of nuclear factor kappa B (NF-?B) signal transcription pathway in the pathogenesis. Methods Fifty-four rats were randomly divided into 3 groups: control group (A group, n=6), model group (B group, n=24), low-dose HC treatment group(C group, n=24). The septic rat model was established by intra-peritoneal injection of LPS (1 mg/kg), the intervention was by caudal vein injection of low-dose HC (6 mg/kg), each of B and C group was subdivided into 2,8,16,24 hours after LPS injection (n=6).At serial time points,the animals in each group were sacrificed , brain tissue samples were harvested to determine NF-?B,I?B expression in hippocampus by immunohistochemistry. Also, the changes of behaviors were observed and the histopathological changes were investigated by Nissl stain.Results In model group (B group): lethargy, anorexia, tachypnea, piloerection, loss of body weight were all more obvious than control groups.Nissl stain showed significant histopathological changes; NF-?B expression was up regulated compared with control group(P