Objective To discuss in vitro the inhibition effect of Pomegranate Rind on H.pylori metronidazole resistant strains and susceptible strains.Methods The Agar Dilution Method detects the minimal inhibitory concentration(MIC) of the water extract of Pomegranate Rind on H.pylori metronidazole resistant strains and susceptible strains,MIC_(50)、MIC_(75)、MIC_(90) were also calculated.Results MIC of Pomegranate Rind on H.pylori was in a range of 7.8125 to 500mg/mL;MIC_(50) of H.pylori metronidazole resistant strains and susceptible strains on Pomegranate Rind was 29.9mg/mL and 28.0mg/mL respectively,MIC_(75) was 65.1mg/mL and 59.1mg/mL respectively,MIC_(90) was 131.1mg/mL and 115.9mg/mL respectively.The susceptibility of H.pylori metronidazole resistant strains and susceptible strains on Pomegranate Rind has no statistical difference(relative median potential= 1.072,95% credible range from 0.763 to 1.513).Conclusion H.pylori metronidazole resistant strains and susceptible strains was susceptible to Pomegranate Rind.