Object To study the chemical constituents of Hibiscus mutabilis L.Methods Isolation and purification were carried out on silica gel, or polyamide column chromatography etc. Constituents were identified and structurally elucidated by physicochemical properties and spectral analysis.Results Ten compounds were obtained, nine of them were determined as tetracosanoic acid (Ⅰ), ?-sitosterol (Ⅱ), daucosterol (Ⅲ), salicylic acid (Ⅳ), emodin (Ⅴ), rutin (Ⅵ), kaempferol-3-O-?-rutinoside (Ⅶ), kaempferol-3-O-?-robinobinoside (Ⅷ) and kaempferol-3-O-?-D-(6-E-p-hydroxycinnamoyl)-glucopyranoside (Ⅸ).Conclusion All compounds are isolated from the plant for the frist time except Ⅱ and Ⅵ.

Object To develop a method for the quantitative analysis of peiminine, imperialine and imperialine 3? D glucoside in Fritillaria L by capillary electrophoresis Methods 9 species of bulbus Fritillaria L. were analysed with internal standard by the mode of capillary zone electrophoresis Results The 3 alkaloids were separated completely with the method which showed good recovery and reproducibility Conclusion The method was proved to be quick, simple, and efficient, and provided a reliable basis for the quality control and evaluation of this plant medicine

Objective To study the chemical constituents of Swertia decora, a traditional herb used to treat hepatitis by the minority.Methods The constituents were separated and purified by column chromatography with silica gel.Their structures were identified on the basis of spectral analysis such as UV, MS, NMR, HMPC, HMQC and by HPLC.Results Three compounds were isolated from the chloroform part and ethyl acetate part of the extracts.Their structures were identified as 1-hydroxy-4, 5, 6, 7-tetra- methoxy -9H-xanthen-9-one (swertiadecoraxanthone-Ⅰ, Ⅰ), 5, 7, 3′, 4′- tetrahydroxyflavone (luteolin, Ⅱ), and 3, 5, 7, 3′, 4′-pentahydroxyflavone (quercetin, Ⅲ), respectively.Conclusion Swertiadecora- xanthone -Ⅰ is a new compound and the other two are found from this plant for the first time.The pentaoxygenated patterns of compound Ⅰ is also found for the first time in the plants of Swertia L.

Object To study the chemical constituents in the lipophilic portion of Panax notoginseng (Burk.) F H Chen Methods Through monitoring each fraction based on antimicrobial experiment of Staphylococcus aureus Rosenbach, the constituents were isolated on silica gel column chromatography and preparative TLC, and the chemical structures were identified by means of spectral data (IR, MS, 1HNMR, 13 CNMR, DEPT, HMQC, HMBC) Results Three compounds were isolated from its petroleum ether extract and identified as: panaxynol (Ⅰ), panaxydol (Ⅱ), and ? sitosterol (Ⅲ) Conclusion Compounds Ⅰ, Ⅱ having strong antimicrobial activity against S aureus were obtained from this plant with the yields of 0.01% and 0.033%