OBJECTIVE:To standardize the operation of monitoring work of the adverse drug reaction in our hospital.METHODS:Based on the newly revised reporting and monitoring administration measures on adverse drug reaction,a detailed specification on a series of operations such as the finding,collection,analyzing,evaluation,feedback,sorting and quantization check of the drug adverse reaction(ADR)and the organization of the drug adverse reaction monitoring in our hospital was formulated.RESULTS:ADR reporting cases increased year by year in our hospital after the specification was formulated.CONCLUSION:This specification is feasible in operation,which can be put into practice well.

To study the treatment of metastasis of experimental lung cancer with a recombinant adenovirus carrying IL 2 gene, concentrations of IL 2 and associated cytokines in murine lung lavage and blood were determined by ELISA at different times after intratracheal injection of the recombinant adenovirus. The lung metastasis nodes, survival time, survival rate were investigated in a C57BL/6 mouse model by intratracheal administration, and the NK activity and CTL activity were also determined by 51 Cr 4 hour release method. The results showed that IL 2 could be detected in lung tissue as early as 6 hours after administration of IL 2 gene, its concentration was higher in lung lavage than in peripheral blood, and was higher in experimental group than that in the control group; intratracheal use of the IL 2 carring recombinant adenovirus had significant therapeutic effect on metastasis of experimental lung cancer. Increased CTL and NK activities, longer survival time, and higher survival rate were observed in the experimental group compared with the control group. It is suggested that intratracheal usage of adenovirus vector carrying IL 2 gene might play therapeutic role on the lung cancer metastasis, indicating that gene therapy of lung tumors could be done through airway directly with recombinant adenovirus.

0.05). From clinical point of view,it is suggested that the weight can be advantageous to forecasting Cr of children.

Objective: To investigate the characteristics of lower respiratory tract infection caused by Chryseobacterium and its antimicrobial susceptibility in vitro . Methods: The clinical data of 52 cases of lower respiratory tract infection caused by Chryseobacterium were analyzed, and the antimicrobial susceptibility of commonly used agents was determined with the method of agar dilution. Also the 50% lethal dosage (LD 50 ) (as the marker of virulence) of 20 randomly selected Chryseobacterium strains for mice were determined. Results: (1) Thirty six was over 60 years old;all of 52 cases had underlying diseases, mainly were chronic obstruction pulmonary disease and malignant tumors. Seventeen cases had the history of incubation or tracheotomy for mechanical ventilation, and 35 had history of broad antibiotics treatment. The mean hospitalization time before infection were 35.6 d, and 38.5% of the cases had mixed infection with other bacteria. No specific clinical manifestations and chest X ray appearance revealed. (2) The in vitro activity of 25 agents showed that these strains were highly resistant. (3) The range of the LD 50 of tested strains was 4.11?10 6 5.68?10 8/mouse, suggesting low virulence of this kind of bacteria. Conclusion: The lower respiratory tract infection caused by Chryseobacterium has no unique features; the incidence of the infection increases in immunosuppressed old patients with various underlying diseases, although the virulence is relatively low. Because the clinical isolates are highly resistant, the antibiotics should be selected according to the results of bacterial sensitivity test.

Objective To compare the differences of salivary alpha(α) amylase (sAA) activity and its activity ratio from the sali‐va before and after citric acid stimulation and approach the correlations among sAA activity determined by the methods of iodine‐starch ,Bernfeld and EPS‐G7 velocity respectively .Methods Ten saliva samples were collected from five healthy volunteers before and after citric acid stimulation .Their activities were determined three times by the three methods ,and the variation coefficient (CV) of sAA activity and activity ratio were calculated .Moreover ,correlation among sAA activities determined by the three meth‐ods were analyzed .Results The significant differences (P< 0 .05) were found in sAA activity and total CV from three determined methods ,and sAA activity and total CV by the method of EPS‐G7 velocity were minimum .There were no significant differences (P> 0 .05) in sAA activities ratio and its CV ;Significant correlation was found between sAA activity determined by random two of three methods(P < 0 .05) ,and their correlation coefficients were above 0 .96 .Conclusion The sAA activity data determined by three methods could be transformed each other by regression equation ,and determined precision by the method of EPS‐G7 velocity is highest ,and data processing method of sAA activity ratio could decrease differences among CV from three methods .

Objective To investigate the influence of comprehensive intervention on improving the quality of life of idiosyncratic infants and young children.Methods A control study Was conducted on 106 idiosyncratic patients aged 6 months to 1 year with specific genetic background,eczema and wheezing under allergen test and comprehensive intervention via a randomized double-blind method.The onset of symptoms and measurement of immunological function were compared after 18 months.Results Serum IgG and IgA increased(all P＜0.05),wheezing and eczema attacks decreased significantly(all P＜0.01),sleep and emotion also improved significantly in the treatment group after 18 months(all P＜0.05).Milk and eggs were the most common allergen for infants and young children.Conclusion Idiosyncrasy and environmental factors were risk factors for allergic diseases.Early childhood was a critical period influencing the occurrence of allergic diseases in children.Early intervention could reduce the incidence of allergic diseases in children and improve the quality of life of idiosyncratic infants and young children.

Objective To investigate the expression and significance of β-catenin and peroxisome prolifera-tot-activated receptor-γ,(PPARγ) in bepatocellular carcinoma. Methods Tissue microarrays were established to detect β-catenin and PPARγ expression in 49 cases of hepatocellular carcinoma,49 cases of adjacent nontumoral liv-er tissue and 6 cases of normal liver tissue. The relationships between PPARγ and β-catenin as well as between PPARγ and clinicopathological parameters were observed. Results The aberrant expression rate of β-catenin was 69.39%,48.98 % and 0 respectively (P=0.001). The positive expression rate of PPARγ was 51.02%,30.61% and 0 respectively (P=0.016). Clinicopathological analysis revealed that the increase of PPARγ expression was not associated with age,tumor size,serum alpha fetoprotein (AFP) levels,tumor embolus of portal vein or inferior vena cava,and HBsAg infection(χ2=0.214,3.201,0.046,3.201,P>0.05 for each),but correlated with differentiation grades(χ2=4.693,P<0.05). Aberrant expression of β-catenin was associated with PPARγ expression(χ2= 5.130,P<0.05). Conclusion Aberrant expression of β-catenin may involve in the liver carcinogenesis. The high expression of PPARγ in hepatocellular carcinoma is significantly correlated with the clinicopathological characteris-tics. Detection of PPARγ is valuable for diagnosing hepatocellular carcinoma,and evaluating malignancy extent and prognosis.

Objective: To investigate the effect of CpG motif oligodeoxynucleotides (CpG ODN) on the antigen induced allergic airway reaction in mice. Methods: The asthma model was set up in the C57BL/6 mice with OVA, the CpG ODN in the dose of 30 ?g was co administered intraperitoneally with the antigen in sensitization stage to study its effect on the airway allergenic reactions. Results: (1)Compared with the control, coadministration of CpG ODN in sensitization phase significantly inhibited airway eosinophilia after antigen challenge( P

OBJECTIVE:To manufacture non-PVC multilayer extrusion film infusion bag and establish its quality standard METHODS:Polypropylene(PP),Polyethylene(PE) and Polyamide(PA) were mixed in proportion and extruded into film Infusion bag was prepared with the film Accroding to the demend of bulk infusion,its quality standard was established RESULTS:The infusion bag was good in quality,simple in preparation technic with no need of expensive facility CONCLUSI_ON:Non-PVC multilayer extrusion film infusion bag is worth further investigating

Objective To compare the curative effect of perindopril and metoprolol in the treatment of diabetic patients with asymptomatic cardiac insufficiency.Methods According to the method of random numbers, 152 diabetic patients with asymptomatic cardiac insufficiency were divided into the observation group and control group,with 76 cases in each group.The observation group was given with perindopril treatment,while control group was given with metoprolol treatment.The cardiac function index,glycosylated hemoglobin,BNP levels,the incidence of cardiac insufficiency and the occurrence of adverse drug reactions before and after treatment were compared.Results There were no significant differences in LVEF,Tei index and E/A between two groups before treatment (all P >0.05 ).Compared with before treatment,the cardiac function indexes of the two groups were significantly improved after treatment(t=5.96,4.83,4.91,10.22,5.68,5.18,all P<0.05),but the observation group were improved more significantly(t=5.64,4.67,5.03,all P<0.05).Before and after treatment,there was no significant difference in the levels of glycosylated hemoglobin(all P>0.05),and the difference between the two groups was not statistically significant(all P>0.05).The BNP levels of the observation group before and after the treatment were (515.43 ± 58.85)pg/mL and (214.43 ±32.42)pg/mL respectively,of which the control group were (513.94 ±59.11)pg/mL and (263.32 ±43.31)pg/mL respectively,and after treatment,the BNP levels of the two groups were significantly decreased(t=23.45,17.68,all P<0.05),of which the observation group decreased more obvious(t=6.97,P<0.05).The cardiac insufficiency incidence of the observation group was 3.95%,which was significantly lower than 14.47% of the control group(χ2 =5.03,P<0.05).The incidence of adverse drug reactions had no obvious differ-ences in both groups(χ2 =0.08,P>0.05).Conclusion Using perindopril in treatment of diabetic patients with asymptomatic heart function can obviously improve cardiac function,reduce the incidence of BNP and cardiac insuffi-ciency.It has the clinical curative effect and high safety.