ObjectiveTo explore the curative effect of Qufengqingre method in treatment of the patients with cough after cold.Methods74 patients with cough after cold were selected and randomly divided into the control group(36 cases) and the observation group (38 cases ).The control group was given roxithromycin (150 mg every time,two times every day),ketotifen( 1 mg every time,one time every night),pentoxyverine citrate(50mg every time,3 times every day).The observation group was given Qufengqingre decoction,and the decoction was boiled in water twice per dose,and mixed juice about 300 ml was abtained for morning and evening oralling.The efficacy,symptoms score and adverse reactions were observed.ResultsIn the control group,7 patients were cured,effective in 21 cases,invalid in 8 cases,and in the observation group,17 cases were cured,effective in 18 cases,invalid in 3 cases.The total effective rate of the observation group was 92.1％ higher than 77.8 ％ of the control group ( x2 =4.16,P ＜ 0.05 ).The cough and sputum score of the two groups before treatment was ( 2.18 ± 0.73 ) points and ( 1.09 ± 0.27 ) points,(2.26 ± 0.65 ) points and ( 1.12 ± 0.23 ) points,respectively,and after treatment was ( 1.11 ± 0.37 ) points and (0.65 ± 0.16) points,(0.72 ± 0.19 ) points and (0.35 ± 0.08 ) points.Differences in scores between the two groups before treatment were not statistically significant(t =1.29,1.31,all P ＞ 0.05 ) ; In the two groups before and after treatment were statistically significant( t =2.68,2.59,2.43,2.41,all P ＜ 0.05 ) ; After treatment the difference between the two groups were statistically significant( t =2.53,2.37,all P ＜ 0.05 ).Both groups had no obviously adverse reactions.ConclusionQufengqingre method in treatment of the patients with cough after cold had remarkable effect.

The rising ofmetabonomics after genomics and proteomics offers chances for the modernization of TCM.Metabonomics has much in common with TCM in the cognition of human body. Appropriate application of the methodology of metabonomics in TCM, such as TCM differential diagnosis and guidance of clinical treatment, would speed up the integration of TCM and modem life science and technology. Meanwhile the research of COPD in TCM will come into a new stage through the use of metabonomics. This article overviewed the current situation of Chinese medicine using metabolomics technology to raise its theory, science and diagnostic level, and the study of pulmonary-qi deficiency syndrome of COPD with metabonomics.

This article was aimed to study the mechanism of Liu-Wei Bu-Qi (LWBQ) Capsules among rat models of chronic obstructive pulmonary disease (COPD) accompanied with reduced lung function based on MMPs/TIMPs and Th1/Th2. A total of 75 rats were randomly divided into the normal group, model group, LWBQ group, Jin-Shui-Bao (JSB) group, spleen aminopeptidase group. Except the normal group, smoke plus lipopolysaccharide tracheal instil-lation method was applied among rats in other groups to establish COPD rat model. Medication was given on the 28th day after the model was established. The medication was given for 30 days. Observation was given on changes of lung histology, lung function, interleukin (IL)-4, interferon-γ (IFN-γ), matrix metalloproteinases (MMP-9) and MMPs inhibitor 1(TIMP-1). The results showed that compared with the normal group, in the model group, lung tis-sues was damaged, and lung function was obviously reduced, while the level of inflammatory factor such as IL-1β, IFN-γ, Th1/Th2 were obvious increased (P< 0.05 or P< 0.01); while inflammation-inhibiting factors such as IL-4 and IL-35 were obviously decreased (P < 0.05 or P < 0.01); MMP-9 gene and protein expression of lung tissues were obviously increased (P< 0.05 or P< 0.01); TIMP-1 gene and protein expression were obviously decreased (P< 0.05 or P < 0.01). After medication, compared with the model group, in the LWBQ group, IFN-γ and Th1/Th2 were obviously decreased (P< 0.05 or P< 0.01); MMP-9 gene and protein expression were obviously decreased (P< 0.05 or P < 0.01); TIMP-1 expression was obviously increased (P < 0.05 or P < 0.01). Compared with the JSB group and spleen aminopeptidase group, the lung function and TIMP1 gene protein expression were increased, while MMP-9 and Th1/Th2 expression were obviously decreased (P < 0.05). It was concluded that LWBQ Capsules up-regulate IL-4 and TIMP-1, downregulate IFN-γ, Th1/ Th2 and MMP-9 expression, in order to reduce inflammatory response and improve lung function among COPD cases.

Objective] With the method of removing wind, activating blood, removing phlegm and relieving cough, apply Bee-silkworm Zhike Decoction, observe its cure effect on chronic cough. [Method] For 32 cases of chronic cough that had no marked cure effect with WM anti-infection and relieving cough to remove phlegm, apply Bee-silkworm Zhike Decoction to them, for 2w as a course, make evaluation after successive 2 courses. [Result] The average onset time was (9.1 ±4.6)d,the cough average disappearance period (20.3 ±9.1)d,28 cases were cured, 4 had effect, the total effective rate was 100%. [Conclusion] Bee-silkworm Zhike Decoction treating chronic cough has characteristics of quick onset, short course and marked cure effect, worth further study.

In recent years, with the continuous increasing incidence and mortality of lung cancer, traditional Chinese medicine (TCM) researches have made some progress. This article reviewed the research progress in the relationship between TCM syndromes and modern medical staging of lung cancer, with a purpose to provide references for the follow-up related work.

0.05). The targets of Group B were elevated than those of Group A (P0.05). Conclusion HTJQ Capsule can decrease the levels NO and NOS in lung tissues of asthmatic guinea pigs and has preventive therapeutic effect for asthma.

Objective To study the associated gene expression in thymus-dependent lymopholyte of the COPD patients with pulmonary qi deficiency syndrome(PQDS)and pulmonary yin deficiency syndrome (PYDS)by gene chips.Methods Collect peripheral blood of patients with PQDS and PYDS(as experimental samples)and of nomal man(as control samples).By Ficoll method,peripheral blood lymphocyte was collected,then thymus-dependent lymphocyte were extracted and purified by flow cytometry.Total RNA were extracted by one-step technique and purified.Then,synthesis double strand cDNA template from total RNA,transcription of cRNA probe with biotin labeling,subsequently,cRNA sample were fragmented. The gene chip(Human Genome U133 Plus 2.0 Array,38500 genes)was hybridized and scanned.Then fluorescent signal value of gene expressing was obtained,and differential expression genes were sifted. Results There were 45 genes expressed differently among PQDS and normal man,including up-regulated 41 and down-regulated 4;32 genes expressed differently between PYDS and normal man,including up-regulated 19 and down-regulated 13;5 up-regulated genes expressed differently between PQDS and PYDS with normal man.Conclusion Gene chip can be applied to study gene expression profiles effectively and to screen PQDS and PYDS associated genes.

Objective To study the level and difference of cytokine in COPD patients with lung qi deficiency syndrome and lung yin deficiency syndrome. Methods The level of IL-8, IL-1? and TNF-? in COPD patients were detected respectively. There were 30 cases with lung qi deficiency syndrome and 30 cases with lung yin deficiency syndrome, meanwhile, thirty healthy people were selected as the control group. Results The cytokine level of lung qi deficiency syndrome and lung yin deficiency syndrome was significant higher than that of the health people (P

Objective:To observe expression of NK cells in peripheral blood in the patients of lung-Qi deficiency and lung-Yin deficiency. Methods:CD_(16)/CD_(56) were determined by flow cytometry in 58 cases of lung-Qi deficiency,12 cases of lung-Yin deficiency and 20 cases of healthy persons.Results:Activities of NK cells in both the patient of lung-Qi deficiency and the patient of lung-Yin deficiency decreased;There were significant differences in activities of NK cells among the various groups of lung-Qi deficiency syndrome.Conclusion:The immunological function in the patient of lung-Qi deficiency syndrome developed gradually to immunodepression of different degrees from active state at early stage,and then developed to lung-Yin deficiency syndrome,and finally leading to maladjustment of hemeostasis of immunological system.

Objective To observe the effects of Liuwei Buqi capsules on CD4+CD25+forkhead box protein 3+(Foxp3+)regulatory T cells(Tregs),helper T cells(Ths)in patients with chronic obstructive pulmonary disease (COPD). Methods Eighty COPD patients admitted into the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine from January 2012 to May 2013 were randomly divided into a traditional Chinese medicine (TCM)experimental group(40 cases)and a western medicine control group(40 cases);there were 40 research volunteers enrolled as healthy controls. After admission,both the TCM experimental group and western medicine control group were given conventional western medicine comprehensive treatment. Additionally,in the TCM experimental group,Liuwei Buqi capsules were given orally once 3 pills(0.4 g/pill)and twice a day,while in the western medicine control group nifedipine was given,once 10 mg and 3 times a day,30 days constituting one therapeutic course for both groups. BODE evaluation means that the body mass index(B),the degree of airflow obstruction(O),the scores of dyspnea(D)and excercise capacity(E)of COPD patients were evaluated. A spirometer was used to observe the changes in the patient's pulmonary function to detect the dyspnea score. The γ-interferon(IFN-γ),interleukin (IL-4 and IL-17)in serum were detected by enzyme-linked immunosorbent assay(ELISA). The expression of Treg of peripheral blood was detected by flow cytometry. Results Compared with healthy control group,lung function parameters,IL-4,CD4+CD25+Treg and CD4+CD25+Foxp3+Treg of peripheral blood were increased,while dyspnea score,BODE score,the expressions of IFN-γ,Th1/Th2,IL-17 in serum were decreased significantly in the COPD group(P<0.05 or P<0.01). After treatment,compared with those before treatment in the two groups, the forced expiratory volume in 1 second(FEV1),FEV1/forced vital capacity(FVC),IL-4,CD4+CD25+Treg, CD4+CD25+Foxp3+ Treg were all increased,while dyspnea score,BODE score,IFN-γ,Th1/Th2,IL-17 were all decreased(P<0.05 or P<0.01),and the peak expiratory flow(PEF)was decreased in western medicine control group and increased in TCM experimental group. After treatment,the comparisons of ratio of FEV1/FVC〔(78.12±14.96)%vs.(67.52±10.39)%〕,BODE score(1.07±0.72 vs. 1.77±0.74),IL-17(μg/L:40.80±8.97 vs. 48.22±6.51) Th1/Th2(1.05±0.23 vs. 1.42±0.21)and CD4+CD25+Foxp3+ Treg〔(6.61±2.26)% vs.(5.25±2.03)%〕between the two groups were all of statistical significant difference(all P<0.05). Conclusion Liuwei Buqi capsules can improve COPD symptoms by up-regulating the expressions of CD4+CD25+Foxp3+ Treg,IL-4,and down-regulating the expressions of IFN-γ,IL-17 to correct the balance of Th1/Th2 in patients with COPD.