China ; Humans ; Orthopedics ; methods ; trends ; Traumatology ; methods ; trends

Arthroplasty, Replacement, Hip ; Femoral Neck Fractures ; surgery ; Fracture Fixation, Internal ; Humans

The venom of Naja naja atra from Guangdong province has been separated into 27 fractions by CM-Sephadex C-25 column chromatog-raphy. One lethal toxin fraction was further purified by ion-exchange rechromatography on CM-Sephadex C-50 and by gel filtration on Sep-hadex G-50 and was named fraction XVII-B2. It can abolish the responses of chikea biventer cervisis muscle to indirect stimulation or to Ach. but not affects the responses to direct stimulation Or K+. On the other hand, it has no effects on Straub's frog hearts in vitro,but its LD50 for mice is about 75ug/kg.Slab-PAGE, SDS-PAGE and isoelectrofocusing were performed to test the homogeneity of the fraction. Some immunological methods were also used. The molecular weight and pl for this fraction are about 7200 daltons by SDS-PAGE and pH 8.5 by isoelectrofocusing. The resemblance between the fraction and cobratoxin has been analysed by comparing the amino acid composition. We have used it successfully in radio ligand assay of nicotinic receptor preparations from Narcine maculata electric organ.The experiment data show that the fraction may be a short post-synaptic neurotoxin and may be an effective tool In studies of acetyl-choline receptor.

Recently, the clinical trials of combined immunotherapy for advanced hepatocellular were completed successfully (Imbrave150, Keynote524), which have significantly increased the objective response rate and prolonged median overall survival time. The success of combined immunotherapy provides great potential and reality for subsequent surgical intervention of advanced hepatocellular carcinoma. Nowadays, the hot topics of combined immunotherapy include the choices of combined immunotherapy, the perioperative period determination, tumor immune microenvironment, and effective biomarkers of immunotherapy selection, and so on. This paper intends to summarize the above-related issues.

Chronic Disease ; Combined Modality Therapy ; Endoscopy ; Humans ; Postoperative Period ; Rhinitis ; surgery ; therapy ; Sinusitis ; therapy

Epigenesis, Genetic ; Humans ; Rhinitis, Allergic ; therapy

Contraindications ; Desensitization, Immunologic ; Humans

Objective:To investigate the clinical value of serological test indicators in the identification of primary and metastatic liver cancers. Methods:We detected the serum levels of ALT, AST, ALP, TBIL, DBIL, GGT, CHE, 5'-NT, CA199, CEA, and AFP in primary hepatic carcinoma (PHC;120 cases), metastatic hepatic carcinoma (MHC;135 cases), and no liver metastatic control (135 cas-es) groups. The methods used were variance analysis and Scheffe test. ROC curve analysis was used to determine the diagnostic value of CA199 , CEA, and AFP in PHC and MHC. Results:The difference between the serum levels of AST, ALP, GGT, 5'-NT, AFP, and CEA of the PHC and MHC groups was statistically significant (P<0.05). The AFP, CEA, and CA199 areas under the ROC curve of the PHC and MHC groups revealed that AFP diagnosis of primary liver cancer had certain accuracy, whereas CEA and CA199 have some diagnostic value in differentiating primary and metastatic liver cancers. Conclusion: The detection of serum levels of AST, GGT, 5'-NT, AFP, CA199, and CEA in malignant tumor was a preliminary diagnosis of liver metastasis and can provide evidence for the dif-ferential diagnosis of PHC and MHC.

AIM:To evaluate the role of inhaling N 6-phenyl-2R-isopropyl-adenosine (R-PIA) on the airway resistance and pulmonary compliance of experimental asthmatic guinea-pigs. METHODS: Experimental model of asthmatic guinea-pigs were made. Inhaling R-PIA 5 mg/mL( 5 mg R-PIA in 1 mL 0.9% saline). The airway resistance, pulmonary compliance and NO- x, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP),tumor necrosis factor (TNF) in bronchoalveolar lavage fluid (BALF) were observed. RESULTS: Compared with asthma group, after inhaling R-PIA, the airway resistance of experimental asthmatic guinea-pigs increased ( P 0 05). CONCLUSION: Inhaling R-PIA could constrict the airway of experimental asthmatic guinea-pigs.

The paper first analyzes the intrinsic need for cultural integration in hospital regrouping and sums up its content, including the integration of values, management ideas, institutional cultures and institutional frameworks. It then introduces some methods of cultural integration in hospital regrouping, such as running in on the basis of respecting human nature, resolving conflicts on the basis of being mutually tolerant, and merging completely on the basis of optimizing means of integration. The paper points out that success or failure of cultural integration in hospital regrouping depends on the thinking of the leaders. For this reason, it is imperative to enhance integration awareness and improve integration capability.