Arthroplasty, Replacement, Hip ; Femoral Neck Fractures ; surgery ; Fracture Fixation, Internal ; Humans

China ; Humans ; Orthopedics ; methods ; trends ; Traumatology ; methods ; trends

The venom of Naja naja atra from Guangdong province has been separated into 27 fractions by CM-Sephadex C-25 column chromatog-raphy. One lethal toxin fraction was further purified by ion-exchange rechromatography on CM-Sephadex C-50 and by gel filtration on Sep-hadex G-50 and was named fraction XVII-B2. It can abolish the responses of chikea biventer cervisis muscle to indirect stimulation or to Ach. but not affects the responses to direct stimulation Or K+. On the other hand, it has no effects on Straub's frog hearts in vitro,but its LD50 for mice is about 75ug/kg.Slab-PAGE, SDS-PAGE and isoelectrofocusing were performed to test the homogeneity of the fraction. Some immunological methods were also used. The molecular weight and pl for this fraction are about 7200 daltons by SDS-PAGE and pH 8.5 by isoelectrofocusing. The resemblance between the fraction and cobratoxin has been analysed by comparing the amino acid composition. We have used it successfully in radio ligand assay of nicotinic receptor preparations from Narcine maculata electric organ.The experiment data show that the fraction may be a short post-synaptic neurotoxin and may be an effective tool In studies of acetyl-choline receptor.

Recently, the clinical trials of combined immunotherapy for advanced hepatocellular were completed successfully (Imbrave150, Keynote524), which have significantly increased the objective response rate and prolonged median overall survival time. The success of combined immunotherapy provides great potential and reality for subsequent surgical intervention of advanced hepatocellular carcinoma. Nowadays, the hot topics of combined immunotherapy include the choices of combined immunotherapy, the perioperative period determination, tumor immune microenvironment, and effective biomarkers of immunotherapy selection, and so on. This paper intends to summarize the above-related issues.

Epigenesis, Genetic ; Humans ; Rhinitis, Allergic ; therapy

Chronic Disease ; Combined Modality Therapy ; Endoscopy ; Humans ; Postoperative Period ; Rhinitis ; surgery ; therapy ; Sinusitis ; therapy

Contraindications ; Desensitization, Immunologic ; Humans

Asthma ; Child ; Cough ; Humans ; Rhinitis, Allergic, Perennial ; complications ; diagnosis ; therapy

Objective: For preparing the reference material of alkaline phosphatase(ALP), the purification and properties of ALP from porcine kidney were studied. Methods: The ALP purification procedure included isolation microvillus of porcine kidney cortices by solubilization the membrance-binding enzymes with 1-butanol, precipitation with ammonium sulfate, chromatography on DEAE-Sephacel,ConA-Sepharose,Sephadex G-200 and a immuno-affinity column of anti-GGT antibodies. Results: The purified enzyme had a specific activity of 402 kU/g protein at 37℃and was almost free of contaminating enzymes. The apparent Michaelis constants was 1.35 mmol/L, and the optimum pH was 10.40. Conclusion: The kinetic properties of the preparation were very close to those of the enzyme present in the human serum, The product was suitable as enzyme preparation for producing the enzyme reference material.

The paper first analyzes the intrinsic need for cultural integration in hospital regrouping and sums up its content, including the integration of values, management ideas, institutional cultures and institutional frameworks. It then introduces some methods of cultural integration in hospital regrouping, such as running in on the basis of respecting human nature, resolving conflicts on the basis of being mutually tolerant, and merging completely on the basis of optimizing means of integration. The paper points out that success or failure of cultural integration in hospital regrouping depends on the thinking of the leaders. For this reason, it is imperative to enhance integration awareness and improve integration capability.