Necrotizing pancreatitis (NP) is a complicated and challenging condition due to the incidence of NP-related complications over the course of disease. As a key role of MDT team, how to properly select the indications and timing of interventions, thereby avoiding insufficient or excessive interventions for patients, has become the key and challenging task in clinical treatment for surgeons. In addition, under the background of diversified intervention models for pancreatitis, how to choose individualized and professional treatment regimens according to the disease progression characteristics of patients, effectively control secondary injury and improve the overall cure rate are also the top priorities of existing treatment for NP. In this article, surgical interventions for NP-related complications were illustrated combined with clinical practice, aiming to improve clinical prognosis of these patients.

Axilla ; Breast Neoplasms ; pathology ; surgery ; Female ; Humans ; Lymph Node Excision ; Lymph Nodes ; pathology ; surgery

Chronic Disease ; Combined Modality Therapy ; Endoscopy ; Humans ; Postoperative Period ; Rhinitis ; surgery ; therapy ; Sinusitis ; therapy

Epigenesis, Genetic ; Humans ; Rhinitis, Allergic ; therapy

Acute Disease ; Adult ; Benzene ; poisoning ; Humans ; Male ; Multiple Organ Failure ; chemically induced ; Occupational Diseases ; complications

Adult ; Craniotomy ; methods ; Humans ; Male ; Nasal Cavity ; pathology ; Paranasal Sinuses ; pathology ; Skull Base Neoplasms ; surgery ; Teratoma ; surgery

Contraindications ; Desensitization, Immunologic ; Humans

Objective To evaluate and compare the diagnostic validity of MRI and DSA in patient suspected with spinal dural arteriovenous fistula(SDAVF). Methods A retrospective review on clinical data of 25 cases diagnosed as SDAVF was done to analyze the characteristics of SDAVF on both MRI and DSA. Results The sagittal or cornoral plane of the T 2 -weighted MRI could best show the extraspinal vascular abnormalities and secondary anomalies, while the orifices of the SDAVF, feeding arteries, draining veins could be satisfactorily demonstrated on the DSA. Conclusions The diagnosis of SDAVF can be made in directly on DSA findings, MRI can delineate the extraspinal vascular abnomalities and secondary anomalies but not the whole picture.

Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.Methods: Hela cells were transfected with the recombinant plas- mid pGenesil-1-BIRC71,pGenesil-1-BIRC72 and pGenesil-1-HK via Lipofectamine~(TM)2000.The expression levels of Livin was determined in Hela cells before and after transfection by fluorescence quantitative real-time PCR and Western blotting. The apoptosis rate of cells was determined by FCM 24,48 and 72h after transfection.Results: The transfection efficiency at 48h was higher than those at 24 and 72h.After transfection with pGenesil-1-BIRC71 and pGenesil-1-BIRC72,gene and protein levels of Livin were significantly reduced(P

Brucella organisms are facultative intracellular bacteria capable of surviving inside professional and non-professional phagocytes.Upon cell contact the bacteria is internalized via receptor molecules.Once inside cells,Brucella localizes in early phagosomes,where it avoids fusion with late endosomes and lysosomes.Then,the bacterium redirects its trafficking to autophagosomes and finally reaches the endoplasmic reticulum,the replicating niche.Once inside the endoplasmic reticulum,Brucella extensively replicates without restricting basic cellular functions or inducing damage to cells.Invasion,intracellular trafficking and replication of Brucella organisms in professional and non-professional phagocytes and the molecular determinants involving Brucella intracellular life are reviewed in this article.