Aim To investigate the expression of SREBP-1(sterol regulatory element binding protein-1) in the kidney of type 1 diabetic rats and the effect of insulin.Methods The type 1 diabetic models were induced by high dose of STZ and rats were randomly divided into three groups: normal control group,diabetes control group and insulin treated group.At the 2nd week end,the triglyceride(TG) content in the kidney of experimental rats was measured by the assay kit and oil Red O staining.Furthermore,the expression of SREBP-1 protein was detected by the methods of Western blot and immunohistochemistry.The analysis of SREBP-1 mRNA was performed by in situ hybridization.Results Compared with the control group,the type 1 diabetic rats' renal triglyceride content markedly increased,and the result of Oil Red O showed that lipid deposited in the renal tubular epithelium.Triglyceride content markedly decreased after insulin treatment.The difference had statistic meaning,compared with the diabetes model group.Immunohistochemistry presented the results that SREBP-1 protein was up-regulated in renal tubular epithelium of diabetic rats and insulin treatment suppressed the increasing.The results of western blot showed that the precursor and mature segments of SREBP-1 protein in kidney of diabetes group rats were about 1.86 times and 1.77 times respectively of that of normal control group rats.In situ hybridization confirmed the increasing of SREBP-1 mRNA in renal tubular epithelium in diabetic rats.The effect of insulin treatment on SREBP-1 expression was detected by the methods of Western blot and in situ hybridization and it was found that the SREBP-1 mRNA and protein of kidney were down-regulated.Compared with the normal group,the difference has statistic meaning(P

Objective To investigate the association of the tumor necrosis factor ligand superfamily member(TNFSF)4 gene polymorphisms rs3861950 and cerebral infaretion in Hunan area.Methods The frequencies of the genotypes of rs3861950 were detected utilizing real-time fluorescent PCR method based on TaqMan probe.Subiects examined were composed of 287 patients and 285 healthy individuals.Results There were significant differences in TNFSF4 gene rs3861950 C→T site polymorphism and allele frequency between the subjects and the controls,the distribution of CC genotype was significantly higher in the former (7.7%)than in the later(2.1%,X2=9.553,P=0.008),so was the frequencies of C allele(0.190 vs 0.137,X2=5.887,P=0.015).Moreover,there were significant differences in the distribution of genotype and the frequencies of allele between the subgroup of cerebral thrombosis and control group ( for the distribution of genotype X2=9.396 P=0.009,for the frequencies of allele X2=6.134,P=0.013).Logistic regression analysis showed that the risk factor of CC genotype was 3.7 times higherthan others(P=0.002.OR 3.706).Conclusions TNFSF4 gene SNP rs3861950 is associated with cerebral infarction and C allele is considered to be one of independent risk factors in the Han population in Hunan Province.