Prolonged sitting is one of the factors for back discomfort. Sitting in the same position for long periods of time is worse. This experimental based study was conducted to achieve three objectives. There were to 1) identify whether is there any differences between perceived body discomfort among respondents before and after upright sitting, 2) identify whether is there any differences between perceived body discomfort among respondents before and after slump sitting and 3) compare the level of perceived body discomfort among respondents after two sitting postures. Thirty young and healthy adults were recruited as study respondents. Each respondent was asked to sit in a posture either upright or slump for 30 minutes. Respondents were then rated their body discomfort using Borg CR-10 scale before and after sitting. Another sitting posture was carried out after one day interval. There is a significant difference between perceived body discomfort among respondents before and after upright and slump sitting. Body discomfort of upright sitting was shown significantly greater than slump sitting. Although, slump sitting caused less discomfort than upright, but it proven by previous studies did not provide benefit to occupational safety and health practice in preventing occupational health related disease.
Upright sitting ; slump sitting ; back discomfort ; safety and health ; injury prevention

Diplazium esculentum is an edible fern commonly consumed by the local community in Malaysia either as food or medicine. Isolation work on the ethyl acetate extract of the stem of D. esculentum resulted in the purification of two steroids, subsequently identified as stigmasterol (compound 1) and ergosterol5,8-endoperoxide (compound 2). Upon further testing, compound 2 displayed strong inhibitory activity against the Plasmodium falciparum 3D7 (chloroquine-sensitive) strain, with an IC50 of 4.27±1.15 µM, while compound 1 was inactive. In silico data revealed that compound 2 showed good binding affinity to P. falciparum-Sarco endoplasmic reticulum calcium-dependent ATPase (PfATP6); however, compound 1 did not show an antiplasmodial effect due to the lack of a peroxide moiety in the chemical structure. Our data suggested that the antiplasmodial activity of compound 2 from D. esculentum might be due to the inhibition of PfATP6, which resulted in both in vitro and in silico inhibitory properties.