Objective To investigate the result of diagnosis and surgery of stageⅠnon-small cell lung cancer (NSCLC).Methods The survival of 47 stageⅠNSCLC patients performed surgery were retrospectively analyzed.Results There were 21 cases who were diagnosed by routine physical examination.The 1～,3～,5～year survival rates of the group of lobectomy were 93.8%,79.3% and 54.6% respectively.The1～,3～,5～year survival rates of wedge resection were 75.7%,42.6% and 35.7% respectively.There were statistical significances of the survival rates between the two groups(P

Objective To investigate the clinical significance of pre-operative ancl intra-operative insulinomas localization, and improve the diagnostic accuracy. Methods 75 patients with suspected insulinomas who were treated surgically were enrolled; all the patients had pathological evidence of insulinomas. The data of pre-operative ultrasound, CT, MRI and intra-operative ultrasound and surgical palpation were collected and analyzed. Results The sensitivity of localization procedures was as follows: ultrasonography 30.7% ( 23/75 ), CT 40.0% (24/60) , MRI 45.4% ( 22/48 ) , while surgical palpation was 80.4% , intra-operative ultrasound 96.4%. The accuracy of intra-operative procedures was higher than that of pre-operative procedures. Conclusions It was difficult to accurately localize insulinomas before operation, but intra-operative palpation and iutra-operative US was easy to perform and highly accurate. Therefore, too much emphasis should not be placed on pre-operative imaging tests.

Objective To research the relationship between binocular acuity and monocular acuity,and their effect on the evaluation of visual impairment and disability. Methods 1006 patients, aged between 18 and 60 years, are measured monocular and binocular acuities after the best lens correction. Results Binocular vision is higher than, equal to and less than monocular vision, three accounted for 27.14%, 70.78% and 70.78% respectively. Interocular difference has certain correlation with binocular interactions (χ2=54.939,P<0.05,r=0.228). Thirty-three patients have same binocular acuity but different monocular acuity. Conclusion Binocular acuity associated with the monocular acuity and the better eye vision plays a special role in visual disability evaluation.

By the breeding model of physics mutagenests (60Co,UV light) and re-strong in the body of insects, We obtained a Bt strain Bt99985 with high insecticidal activity. The shake flask and 40t tank fermentation tests showed that, this strain showed special activity against Spodoptera exjgua, Comparing with original Bt strain: 93005, CK strain: HD-1-580 and GC-91, the value of potency increased respectively by 429%, 655% and 114% in shake flask test. Comparing with GC-91, the value of potency increased by 180% in 40t tank fermentation test.

Object To determine the activities of various antioxidant enzymes, including superoxide dismutase, catalase, peroxidase and glutathione peroxidase, and the contents of some simpler antioxidants such as glutathione, chlorogenic acid and certain flavonoids in the leaves, stems and fruits of Viscum coloratum (Kom ) Nakai Methods The activities were determined as described in various literature reports Results The plant was found to be rich in various antioxidants Conclusion V coloratum, with its abundant antioxidant constituents, is proved to be a strong antioxidation drug for medical use especially its leaves

OBJECTIVETo explore the anti-tumor effects induced by fusion of interleukin (IL)-18 gene transfected lung cancer cell line NCI-H460 cells with dendritic cells (DC).

METHODS(1) DC were induced from human monocytes and fused with IL-18 transfected NCI-H460 cells. Fusion was selected using MACS microbeads. (2) Four groups (group GT, group PT, group NT and group BC) were set up. T cells activated by IL-18 gene transfected fusion or pcDNA3. 1 + vector transfected fusion and non-transfected fusion were taken as effetor cells. No effector cells was in group BC. Lactic dehydrogenase ( LDH) method was used to evaluate the antitumor effect in vitro. (3) Tumor-bearing nude mice were inoculated with effector cells mentioned above. The tumor size and weight in the 4 groups were compared.

RESULTSThe killing rate in vitro of 3 groups were 53. 14% ,30. 10% and 31.49% , respectively. The tumor size and weight in the 3 groups were lower than group BC, among which group GT was the lowest.

CONCLUSIONFusion of IL-18 gene transfected NCI-H460 lung cancer cells with dendritic cells can effectively induce anti-tumor immunity in the host.

Animals ; Cancer Vaccines ; administration & dosage ; genetics ; immunology ; Cell Fusion ; Cell Line, Tumor ; Cell Proliferation ; Cells, Cultured ; Dendritic Cells ; cytology ; immunology ; Female ; Humans ; Immunotherapy, Adoptive ; methods ; Interleukin-18 ; genetics ; metabolism ; Lung Neoplasms ; immunology ; pathology ; therapy ; Lymphocyte Activation ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; T-Lymphocytes ; cytology ; immunology ; T-Lymphocytes, Cytotoxic ; immunology ; Transfection ; Xenograft Model Antitumor Assays ; methods

OBJECTIVETo study the absorption and transformation of N, P, K and plant growth as well as the accumulation of active ingredients in Angelica dahurica var. formosana from Sichuan province and provide basis for rational fertilization.

METHODSamples were collected in different growth phrases, and correlative morphological indexes, the contents and accumulations of N, P, K and active ingredients were determined.

RESULTThe vegetative growth period of A. dahurica var. formosana sowed in autumn could be preliminarily divided into three phases: seedling period, aerial-part peak growth period and root peak growth period. With the development, the plant height and aerial part dry weight increased at the earlier stage, and then decreased. But the root length, root diameter, and root dry weight increased. The absorptive capacity of N, P and K varied among different developing stages. In general, the absorptive capacity of N and K was the higher than that of P. The order of total nutrition accumulation was N > P > K, the accumulation of N, P and K in aerial part decreased gradually during the growth period, while the accumulation of N, P and K in root increased gradually. The concentration of imperatorin and isoimperatorin showed decreasing-increasing-decreasing-increasing trend, while the accumulation of them increased gradually. And the correlation analysis results showed that the accumulation of P and K in aerial part had positive correlation with the concentration of imperatorin in root, the accumulation of N, P and K in root had positive correlation with the concentration of isoimperatorin in root.

CONCLUSIONFrom late April, A. dahurica var. formosana from Sichuan province entered root peak growth period and the accumulation of N, P, K, imperatorin and isoimperatorin in root increased rapidly. So the balanced application of fertilizer should be considered.

Angelica ; chemistry ; growth & development ; metabolism ; China ; Fertilizers ; analysis ; Nitrogen ; analysis ; metabolism ; Phosphorus ; analysis ; metabolism ; Potassium ; analysis ; metabolism

Objective By observing the effect of Xiaoyuhuatan decoction on insulin resistance in rar with nonalchoholic fatty liver,the author made a reasearch into this herb'S machanism on cambating on nonalchoholic fatty liver.Methods The insulin resistance rat model was fed by high fat forage and Dongbaogantai control group was set up for the sake of comparion.We determined the contents of total cholesterol(TC),triglyceride(TG),free fatty acid(FFA),fasting plasma glucose(FBG),fasting serum insulin(INS)in serum and the contents of TC,TG of liver tissue homogenate of each group,and the degree of hepatocytic steatosis.Results Xiaoyuhuatan decoction could obviously decrease the index of insulin resistance and improve insulin insensitive index.Lipid in blood serum and hepatic tissues were significantly decreased,liver fat cell denaturation was improved.Conclusion The thrapentic eftect of Xiaoyuhuatan Decoction on fatty liver is probably contribute tO this herb's mechanism of improving insulin resistance and increasing insulin insensitive.

To identify the prevalence and the distribution of ticks infected with spotted fever group rickettsiae (SFGR) in Xunke Area of Heilongjiang Province ,China ,partial outer membrane protein A gene (ompA) and citrate synthase gene (gltA) specific fragments were assessed using the PCR method .The positive products were sequenced .Result showed that the pres-ence of SFGR was 14 of 60 in detection Dermacentor silvarum cases ,while the overall positive rate was 23 .33% .Its nucleotide sequence of ompA showed 99 .3% and 99 .18% similarity with nucleotide sequence of Rickettsia sp .JL-02 and Rickettsia rao-ultii respectively .And the evolutionary positions of SFGR species were similar with Rickettsiamontana and Rickettsiamassili-ae .It's concluded that the nature focus of tick-borne spotted fever did exist in the area of Xunke Area of Heilongjiang Province , China .

Objective To isolate and culture sweat gland epithelial cells in vitro,and to study the effects of acetylcholine (ACh) on intracellular flee calcium concentration ([Ca~(2+)]i) of cultured sweat gland epithelial cells.Methods Sweat glands epithelial cells were collected by enzymatic digestion.After ACh was added to the primary and first passage cells,[Ca~(2+)]i was examined using confocal laser scanning microscopy (CLSM) and the Ca~(2+) sensitive dye Fura 3/AM.Results The primary and first passage epithe- lial cells grew well.After ACh was added,opening of the calcium channel and significant [Ca~(2+)]i increase were observed when the primary and first passage cells were incubated with high concentration of calcium (2 mmol/L);no significant [Ca~(2+)]i increase was observed in those cultured without calcium.Conclusion Upon stimulation with ACh,calcium channels of cultured primary and first passage sweat gland epithelial cells would open,influx of extracellular Ca~(2+) occurred,which resulted in an increase of [Ca~(2+)]i.Extracellular bound calcium was therefore converted into intracellular free calcium.