1.Anti-Inflammatory Effect of Omega-3 Fish Oil Accelerates MyoD and Myogenin Expressions Following Rat Exercise-Induced Muscle Injury Model in a Dose-Dependent Manner
Idiawati Nurlim ; Junaedy Yunus ; Zaenal Muttaqien Sofro
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 3,June):86-93
Introduction: Exercise-induced muscle injury stimulates production of proinflammatory cytokines, e.g., TNF-α, resulting in impaired satellite-cell-dependent muscle regeneration. Omega-3 fatty acids emerge to possess anti-inflammatory properties. This study aims to analyze the effect of omega-3 fish oil administration on the levels of TNF-α,
MyoD and myogenin expressions of satellite cells after injury. Methods: Twenty-nine adult male Wistar rats were
randomized into five groups. Except for control groups, the rats underwent single bout of downhill running exercise
and three groups were given low-to-high doses of omega-3 fish oil administration 2 hours after exercise. Blood samples were collected after 24 hours to measure the concentration of TNF-α using ELISA and then the soleus muscles
were surgically removed after 72 hours to measure mRNA expressions of MyoD and myogenin using RT-PCR. Results: The results showed lower serum levels of TNF-α (166.83 ± 16.15 vs. 132.83 ± 25.44, 125.00 ± 17.26, 99.66 ±
32.00 pg/mL) and higher expressions of MyoD (0.47 ± 0.19 vs. 0.64 ± 0.20, 1.17 ± 0.16, 1.07 ± 0.14) and myogenin
(0.45 ± 0.10 vs. 1.82 ± 0.35, 1.50 ± 0.34, 0.76 ± 0.20) in groups given low-to-high doses of omega-3 fish oil supplementation, respectively, compared to exercise group with no supplement at 72 hours after exercise. Conclusion: Our
study suggests that omega-3 fish oil supplementation following muscle injury may accelerate myogenin expression
and a low dose of supplementation achieves optimal effect in promoting muscle regeneration. .
2.Subchronic Administration of High-dose Monosodium Glutamate Causes Spatial Memory Dysfunction and Structural Deficits of Rat Hippocampus
Muchtar Hanafi ; Faizal Muhammad ; Amilia Ramadhani ; Nurlaela ; Zaenal Muttaqien Sofro ; Ginus Partadiredja
Malaysian Journal of Medicine and Health Sciences 2023;19(No.4):159-165
Introduction: The toxicity of high concentration monosodium glutamate (MSG) has become a controversial issue
because of its inconsistent results in human and animal studies. This present study aims to evaluate the effect of subchronic high-doses oral administration of MSG on spatial memory performance and hippocampal pyramidal cells
number. Methods: This study involved twenty-eight male Wistar rats, which were divided into a control group of
NaCl 0.9% and three intervention groups of MSG 1.0 mg/g bodyweight (M1), 2.0 mg/g bodyweight (M2), and 4.0
mg/g bodyweight (M3) for 30 days. Statistical analysis used a One-way ANOVA test. Results: The result showed significant differences in spatial memory on the Morris Water Maze (MWM) test, including path length (p = 0.020) and
escape latency (p = 0.011) according to general linear model repeated measurement analysis. The mean difference
of estimated hippocampal pyramidal cells total number among the groups showed volume (p = 0.001), numerical
density (p = 0.590), and cells number (p = 0.004). Furthermore, Post-Hoc analysis in both spatial memory and hippocampal pyramidal cells showed that the increasing MSG dose from 1.0 to 4.0 mg/g bodyweight led to a decrease in
the results of spatial memory performance on the MWM test and a decrease in hippocampal cells. Conclusion: The
present study has provided novel quantitative data that subchronic administration of high-dose MSG caused deleterious effects on the spatial memory function and the volume and number of hippocampal pyramidal cells.