With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.

Humans ; Infant ; Milk, Human ; chemistry ; Paraquat ; poisoning ; Poisoning ; diagnosis

Objective To evaluate the clinical usefulness of MR imaging(MRI) and MR tomography angiography(MRTA) in demonstrating the presence and degree of neurovascular contact of the rostral ventrolateral medulla oblongata(RVLM) in root entry zone of the 9th and 10th cranial nerves in patients with essential hypertension(EHT) and normotensive health volunteers(NTHV).Methods(Patients with) EHT(group 1,n=100) and NTHV(group 2,n=88) underwent high-resolution(axial and coronal) brain stem MRI and MRTA.Images were interpreted consensually by tow radiologists who were blinded to the patients hypertensive status.Neurovascular contact was graded as vessel contact without RVLM deformity(gradeⅠ),clear vessel contact in continuity with the brain stem but without apparent associated deformity(graded Ⅱ), and displacement contact of the RVLM(graded Ⅲ).Results(Neurovascular) contact of RVLM was found in 52.0%(52/100) of EHT and in 43.2%(38/88) of NTHV(?~2=1.459,P=0.230).The compression rate(gradeⅠ—Ⅲ) and affected side(left or right) showed no statistically significant differences between the EHT and NTHV(?~2=0.879,P=0.350;?~2=0.238,(P=0.628);?~2=0.733,P=0.390).Conclusion Neurovascular contact is not more frequently seen in patients with EHT than in normotensive contact subject.This result does no support the hypothesis that neurovascular contact at the RVLM is an etiology of EHT.Furthermore,thin-slice(3 mm) MRI may not be a reliable good screening method for detecting patients with neurovascular contact.Therefore,MRI cannot aid patient selection among hypertension patients lacking symptomatic cranial neuralgias.

Objective: To detect the localization of HBV DNA in kidneys of HBV-associated glomerulonephritis, and investigate the pathogenesis of HBV-associated glomerulonephritis. Methods: 45 cases of renal biopsy specimens (38 cases had glomerular deposition of HBV antigens, 7 cases were negative for serologic and histologic HBV antigens) were examined for HBV DNA by in situ hybridization (ISH) and in situ PCR (IS-PCR). Results: The positive rate of HBV DNA in renal biopsy was 71% (27/38) in patients with glomerular deposition of HBV antigens. HBV DNA was found in 19 cases (19/26, 73%) of HBV-associated glomerulonephritis; and in 8 cases (8/12, 67%) of IgA nephropathy and lupus nephritis with glomerular deposition of HBV antigens. HBV DNA was detected mainly in the cytoplasm of tubular epithelia with ISH; IS-PCR showed that HBV DNA was localized not only in cytoplasm of tubular epithelia, but also in nuclei of tubular epithelia, in nuclei and cytoplasm of glomerular epithelia and mesangial cells, and in GBM. Conclusion: Our study suggested the presence of HBV infection and replication in glomerular cells and renal tubular epithelia, indicating an etiologic role of HBV in HBV-associated glomerulonephritis.

Objective To apply chaos characteristics to prediction of the unilateral mastication.Methods The paper calculated the Largest Lyapunov Exponent of the masseter muscle of some males and females with a method from small data sets,which then was processed by reusable two-factor analysis of variance.Results The results shows that the signal of the masseter muscle has chaos characteristics,the male's Largest Lyapunov Exponent is higher that the female's,and the chaos degree of the masseter muscle on both sides is consistent nearly.Conclusion The Largest Lyapunov Exponent can be used to characterize the signal of the masseter muscle.Comparative Analysis of the Largest Lyapunov Exponent on both sides can be used as reference when to predict and diagnose the unilateral mastication.

Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.

Medical record writing may open up the clinical intern's clinical field of vision his,raise clinical thought,and improve his medical comprehensive ability.The clinical intern in medical record writing has the following questions:the delayedwriting,unstandard language and the unreal content and so on.Through the centralizing teaching,teachers'prompt counsel and interns'initiation and so on,clinical interns'medical record writing quality may be enhanced.

Cognition ; Cognition Disorders ; Humans ; Manganese ; Occupational Exposure

Objectives By Oxford pathological classification and analysis of circulating complement complement 3 (C3),renal C3 deposition,and clinical laboratory tests,we discussed the correlation between complement C3 and immunoglobin A nephropathy (IgAN) in pathogenesis.Methods A retrospective study of 558 IgAN cases at Xinhua Hospital from January of 2000 through December of 2013 was performed.All 558 IgAN diagnoses were made and confirmed by renal needle biopsy.Results Compared to patients who had circulating C3 ＜ 0.9,patients with circulating C3 level ＞ 0.9 showed statistically significant decreases in serum creatinine [(100.92 ± 105.31) μmol/L vs (157.58 ± 208.39) μmol/L,t =-2.283,P =0.025],blood urea nitrogen [(5.69 ± 2.88) mmol/L vs (7.69 ± 5.90) mmoL/L,t =-2.81,P =0.006];besides,other parameters like IgA,body weight,body mass index (BMI),cholesterol,triglyceride,serum IgA/C3 ratio,albumin,and estimated glomeruli filtrate rate (eGFR) also presented statistically significant differences between two patient groups;no statistically significant differences were observed between two groups in glomerular mesangial cell proliferation,capillary endothelial proliferation,segmental glomerular sclerosis or adhesions,renal tubule atrophy,tubulointerstitial fibrosis,and formation of glomerular crescent;meanwhile,no statistically significant differences were observed between two groups in mesangial depositions of IgA,IgG,IgM,and complement C3;meanwhile the blood level of C3 between C3 deposition negative group,deposition 2 + and 3 + subgroup showed statistically significant differences (2.493 and 2.782;0.013 and 0.006),nevertheless,prognostic indices in Oxford classification,such as mesangial cell proliferation,capillary endothelial proliferation,segmental glomerular sclerosis or adhesions,renal tubule atrophy and tubulointerstitial fibrosis,were also statistically different between two patient groups (Pearson Square test result was 50.782,35.141,21.105,30.182,respectively;P ＜0.01).Conclusions Renal deposition of complement C3 or decrease in circulating C3 level may be associated with a poor prognosis of IgA nephropathy,and alteration in C3 dynamics may be implicated in the pathogenesis of IgAN through its involvement in humoral immunity.

Objective To investigate paclitaxel and ezrin-siRNA therapy for metastatic liver cancer.Methods Human metastatic liver cancer cells MHCC97-H were divided into four groups in vitro and in vivo,including control cells,exposure to paclitaxel alone,ezrin-siRNA alone,and the combination of paclitaxel and ezrin-siRNA.Nude mice lung lesions were formed by tail vein injection of tumor cells.The tumor cells'apoptosis rate,wound healing ability,the ability to cross an artificial membrane,and the numbers of metastatic lung lesions were measured.Results The apoptosis rate in normally cultured MHCC97-H tumor cells was 2.52％,compared to the other three groups at 11.66％,2.35％,7.38％ respectively.The wound healing ability was best in the negative control and weakest in cells treated with combination paclitaxel and ezrin-siRNA.The numbers of cells which could cross the artificial membrane in the negative,paclitaxel,ezrin-siRNA,and combination paclitaxel and ezrin-siRNA group were 52.5 ± 3.9,32.2 ± 3.2,26.6 ± 2.4,and 19.4 ± 1.1 respectively.The numbers of metastatic lung lesions in the nude mice (n =40) were 12.5 ±2.4,8.2 ± 1.5,7.0 ± 1.3,and 3.8 ± 0.3 respectively.Conclusions The ezrin-siRNA combined with paclitaxel therapy demonstrated a greater efficacy over single agent therapy for depressing liver cancer growth,motility,aggression,and metastasis in vitro and in vivo.