One thousand (1000) Cases of various thyroid disease using Fine-needle Aspiration Biopsy Cytology, totally 1318 times are discussed in the article. The aocuracy of results is more than 90 percent in accord with the clinical diagnoses. The repeated consistency is more than 70 percent. The accuracy of result in accordance with the pathology examination after thyroid operation is 90 percent Therefore, Thyroid Fine-needle Aspiration Biopsy Cytology is simple, less suffering for patients and higher accurate diagnostic for the evaluation of thyroid disease.

The clonal proliferations of multiple myeloma cells consist of several signaling pathways.It has been shown that cyclic adenosine monophosphate (cAMP) signaling pathway,an important intra-cellular messenger delivery system,is related to abnormal proliferation and apoptosis of malignant lymphoid cells including myeloma cells.Manipulating the cAMP signaling pathway could induce cell cycle arrest and apoptosis of various malignant lymphoid cells including myeloma cells,which involve mitochondrial-dependent apoptosis and cAMP-modulated changes of intra-cellular mediators.In-depth researches and analysis of cAMP-induced apoptosis in myeloma cells would provide potential targets and possible therapeutic means in the treatment of multiple myeloma.

[Objective] The better optimization of traditional Chinese medicine for the treatment of respiratory syncytial virus, in the past five years is re-viewed in this paper, also the related domestic experimental research results, and according to the pathogenesis of RSV, it further summarizes the current domestic research for the main direction of the disease. [Methods] The article from the two aspects of compound Chinese medicine and traditional Chinese medicine monomer, according to the pathogenesis of respiratory syncytial virus section, discusses the experiment in vivo and in vitro related rich research results. [Results] It is found by inducing and summarizing, whether individual or compound traditional Chinese medicine, for respiratory syncytial virus re-search has made great achievement, but with experimental study limited to suppress the virus more value-added; replication and inhibiting inflammatory infiltrates the two big aspects, lacking of deeper side of molecular biology research, making the traditional Chinese medicine in the treatment of respiratory syncytial virus research appear too single, not a broader recognition by the industry. [Conclusion] We need to be sure the safety of traditional Chinese medicine for the treatment of respiratory syncytial virus and curative effect, but at the same time must recognize that because of the complexity of its com-position and design of experiment of traditional Chinese medicine, the irrationality of the existence of many factors, it makes progress with many problems, so we must be aware of their own insufficiency, more in-depth explore its mechanism of action, with al kinds of research direction in the future for us.

100 g/d), hypernatremia and hyperglycemia were the three main risk factors resulting in plasma hyperosmolality. The 4-week survival rate of hyperosmolality group(47.1%) was significantly lower than that of non-hyperosmolality group(78.3%, P

A part of pathogenetic factors of hypertensionin twenty--seven patients with hypertensive NIDDM (group 1) were observed. The control groupsincluded non-hypertensive NIDDM (group 2, n=46 ), primary hypertension (group 3, n= 15) andhealthy persons (group 4, n= 15). The oral glu-cose tolerance test (OGTT), insulin releasing test(IRT ), atrial natriuretic peptide (ANP), an-giotensin I (ATI), aldosterone (ALD), wholeblood volume and urine protein were measured.The results showed that the plasma insulin andsodium levels and the whole blood volume weresignificantly higher in group 1 than those in con-trol group 2, the ANP level was rather lower than that in control group 4. Although the results werecorresponded with the theory that the circulatinghyperinsulinemia may lead to sodium retention andinhibited an adequate ANP stimulation by sodiumchallenge which turn to hypertension, nonhyper-tensive NIDDM patients (group 2) also have thesame findinge. So the retention of sodium and wa-ter can't be used to explain the mechanism of hy-pertension in NIDDM patients, the other patho-genetic factors especialy the factors related to thehyperinsulinemia such as PGE and PGI need to beinvestigated futher.

Objective To find out the difference in expression of glial fibrillary acidic protein and neuron-specific enolase in hippocampi and olfactory bulb of Alzheimer's disease rats,and the relationship between olfactory and Alzheimer's disease.Methods Adult rats,the expression of GFAP and NSE in hippocampi and olfactory bulb was measured by immuhistochemical after a single intracerebroventricular injection of ?-amyloid peptide,and change of these protein in olfactory.Results Comparing the hippocampi and olfactory of model and control,the expression of GFAP in model group increased evidently,the expression of NSE descended animals.Conclusion There is significant difference in olfactory system between Alzheimer's disease model and control group rats.

Objective To investigate the current status of healthcare-associated infection(HAI) management professionals in county and above levels of traditional Chinese medicine(TCM) institutions in a province.Methods The uniform questionnaires were adopted to investigate the basic status of HAI professionals in county and above levels of TCM institutions in a province.Results There were 117 county and above levels of TCM institutions in this province,85 TCM institutions participated the investigation,there were a total of 128 professionals,including 82 professionals in 65 secondary hospitals,and 46 in 20 tertiary hospitals.Among the investigated TCM hospitals,83 (97.65 ％) set up HAI management committees,77 (90.59％) set up independent HAI management departments,55 (64.71％) assigned HAI professionals that met The Requirements of HAI Surveillance Guideline,27 (31.76 ％) exclusively engaged in management of HAI,58 (68.24 ％) were also responsible for other management besides HAI management.Among investigated professionals,91.41％ were nursing staff,55.47％ received undergraduate course or above,54.69％ had intermediate professional titles,61.72％ were at the age of 41-50,45.31 ％ engaged in HAI management for 1-3 years,115 professionals had management certificates.Conclusion The infec tion management in provincial traditional Chinese medicine institutions are understaffed,professional structure is unitary,professional quality is low,leaders in different levels of TCM institutions should enhance HAI management awareness,strengthen the construction of professional quality,and cultivate multi-disciplinary talents as soon as possible.

Background and purpose:Despite the high remission rate in patients with multiple myeloma (MM) after the standard regimen, but often relapsed and resistant. It has been shown that modulation of cAMP can induce cell cycle arrest and apoptosis in a variety of tumor cells, which has become an interesting approach to cancer therapy. This study aimed to investigate possible effects of cyclic adenosine monophosphate (cAMP) analogue 8-(4-chlorophenylthio) adenosine 3’, 5’-cyclic monophosphate (8-CPT-cAMP) on multiple myeloma cells, provide direction to develop new drugs for the treatment of MM. Methods:The myeloma cell line U266 cells were treated with 8-CPT-cAMP of different concentrations. The proliferation of U266 cells was evaluated through cell counting kit (CCK-8) assay, lfow cytometry was used to analyze the changes of cell were distribution, apoptosis rate as well as mitochondrial transmem-brane potential (ΔΨm) in U266 cells before and after the treatment. Meanwhile, real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were used to detect expression levels of apoptosis regulators including caspase-8, caspase-9, Bcl-2 and Bax genes in U266 cells before and after the treatment. Results:The U266 cells were treated 5 days with 8-CPT-cAMP of different concentration, it was shown that 8-CPT-cAMP could signiifcantly inhibit cell growth of U266 cells in a concentration and time dependent manner, the IC50 of 8-CPT-cAMP was reduced obvious prolonged reaction time, and reached to 58.52μmol/L in the iffth day. The cell cycle of U266 cells was stopped in G0/G1 stage as the progress of concentration. It was showed statistical signiifcant difference associated with the cellular proliferation inhibition rate and apoptosis rate in different concentration and control (P<0.05). Meanwhile, 8-CPT-cAMP could induce mitochondrial transmembrane potential collapse in U266 cells. Compared with control groups, the levels of Bcl-2 mRNA transcripts and protein in U266 cells were reduced in 8-CPT-cAMP treated groups (P<0.05), while the levels of caspase-9, Bax mRNA transcription and the expression of Bax protein were increased in treatment groups, but the caspase-8 mRNA had no statistical signiifcant difference with controls. Conclusion:8-CPT-cAMP can inhibit the proliferation and promote apoptosis of myeloma cells, which might be mediated by caspase via mitochondrial pathway.

Objective:To investigate the effect and safety of sertraline tablets with oral administration in the diabetes patients com-plicated with depression. Methods:Totally 74 diabetes patients complicated with depression were randomly divided into 2 groups with 37 ones in each. All the patients were orally given dimethyldiguanide 500 mg,tid,and the observation group was given sertraline tablets additionally,50-100 mg,qd. The treatment course was 8 weeks. The changes of sugar metabolism and the scores of self-rating depres-sion scale( SDS)and quality of life scale( DMQLS)in the two groups were compared. Results:After the 8-week treatment,the blood sugar,SDS scores and DMQLS scores in the two groups were significantly improved when compared with those before the treatment( P<0. 05). The levels of FBG,2hPG and HbAlc and SDS scores in the observation group were significantly lower than those in the con-trol group(P<0. 05),while the scores of DMQLS disease,psychology,society and satisfaction dimension were significantly higher in the observation group(P<0. 05). The incidence of adverse reactions showed no statistically significant difference between the two groups(P>0. 05). Conclusion:Sertraline tablets can improve the depression of diabetes patients and enhance the blood sugar control and quality of life.

Objective The purpose of the present study was to develop an animal model of type II diabetic coro-nary heart disease in Zuker diabetic fatty ( ZDF) rats.Methods The ZDF rat model of type II diabetic coronary heart disease was prepared by high-fat diet feeding combined with continuous injection of isoprenaline hydrochloride (ISO) in a dose of 1 mg· mL-1 for 10 consecutive days.Serum creatine kinase (CK), and creatine kinase izozyme (CK-MB), ST segment in electrocardiogram ( ECG) and myocardial pathological changes were detected to evaluate the rat model.Results CK of both the control and model groups was gradually increased with ISO injections, while CK-MB increased first and then decreased.The ST segment in ECG part II had significant changes.The pathological examination found that about half of the myocardial cross section in the model group was necrotic after injections of ISO for 5 days and more than 3/4 of the my-ocardial cross section was necrotic after injection of ISO for 10 days.The results indicated that ISO caused myocardial inju-ry in ZDF rats.Conclusions The variation of CK-MB, CK, ST segments in ECG and myocardial necrosis indicate that the model is successfully established.The use of high-fat diet combined with continuous injection of isoprenaline hydrochlo-ride in a dose of 1 mg· mL is a simple way to develop a Zuker diabetic fatty rat model of type II diabetic coronary heart dis-ease.