Chromosome 22qll deletion syndrome(22q11DS) is a common chromosomal microdeletion syndrome. Its clinical manifestation is complex, comprising congenital heart disease, dysmorphic facial, immunodeficiency, endocrine dysfunction and so on. The syndrome has a population prevalence of approximately 1/2500-1/4000. There have been many recent advances in understanding of the clinical manifestation, behavior and psychiatric problems and the mechanisms leading to the specific phenotypic features in chromosome 22q11 deletion syndrome. Asymmetric recombination of homologous low copy repetitives in the deletion region causes the deletion of 22q11. TBX1 is the dominant gene contributing to the phenotype.

The immune microenvironment of hepatocellular carcinoma (HCC) is mainly composed of tumor-associated macrophages, myeloid-derived suppressor cells and other cellular components, as well as extracellular components, such as cytokines, growth factors and extracellular matrix, etc. In China, most liver cancer patients are complicated with chronic hepatitis B and cirrhosis. Immune microenvironment promotes the incidence and progression of HCC, immune escape and treatment resistance, and exerts immunosuppressive effect. In recent years, significant progress has been made in immunotherapy for systemic treatment of HCC, such as immune checkpoint inhibitors (ICIs). However, in the KEYNOTE-240 and CheckMate 459 trials, anti-PD-1 therapy with nivolumab or pembrolizumab as a single drug failed to reach the expected overall survival endpoint. At present, it is urgent to deepen the understanding of immune microenvironment of HCC and explore novel therapies to improve clinical efficacy of ICIs. Currently, the combination of ICIs with other therapies (such as tyrosine kinase inhibitors, monoclonal antibodies or local therapy) has been proven to improve the efficiency of single ICIs. In this article, research progress in immune microenvironment, immunotherapy and immune combined with targeted therapy for HCC was reviewed.

Objective To investigate pregnancy complications,cesarean delivery and abnormal fetal presentation in women with uterine anomalies.Methods Fifty women diagnosed with uterine malformation and sixty women with normal uterus were retrospectively analyzed .Results The group comprised women with uterine malformation had significantly higher stillbirth or fetal death (P <0.05) and lower rates of the full-term delivery(P <0.05) than that of the control group.The subgroup comprised women with uterine anomalies had significantly higher rates of cesare -an section(P <0.05) and abnormal fetal position(P <0.05) than the control group.The birth weight and length of infant in observation group were significantly lower than those in control group (P <0.05).The rate of placenta abnormalities in uterine anomalies was significantly higher than that in control group (P <0.05).The incidences of premature rupture of membrane,cord entanglement,oligohydramnios,postpartum hemorrhage and neonatal asphyxia in observation group were no significant difference than those in control group .The rate of premature rupture of membrane(PROM) in uterus unicornis was significantly higher than that in other types (P <0.05).Conclusion Compared with women with normal uterus ,the incidences of abnormal fetal position ,the placenta abnormalities,pre-mature delivery,and stillbirth or fetal death in women with congenital uterine anomalies are high .Uterine anomalies are associated with a decrease in the birth weight and length .We should pay more attention to finding the uterine malformation and treatment before pregnancy .Antenatal care should be reinforced in pregnancy women with uterine anomalies.

Objective To analyze retrospectively MRI features of ectopic pregnancy correlated with pathology.Methods Eleven patients with pathologically proved ectopic pregnancy were enrolled in this study:one with cornual pregnancy,six with interstitial pregnancy,two with tubal isthmus pregnancy,one with ampullary pregnancy,and one with primary abdominal pregnancy.Six under-went MRI plain scan,and five underwent MRI both plain scan and enhancement scan.We evaluated the location,the size,the signal intensity of the gestational sac (GS),vessels around the GS,dilatation tubal,hematosalpinx and hemoperitoneum.Changes of the thickness of the endometrium,functional ovarian cysts and ovarian corpus luteum were also evaluated.Results Among these eleven patients,GSs were detected in ten patients.The locations of the GSs on MRI were corresponding with the results of surgical explora-tion.GS was not detected in only one patient with tubal pregnancy,but hematosalpinx and pelvic hematocele could be seen in MRI. Conclusion MRI is an effective modality for evaluating GS and its adjacent organs.The imaging features of GS and other indirect signs can be helpful to the diagnosis and differential diagnosis of ectopic pregnancy on MRI.

Objective Delivery of oxygen in tissues is limited in the space where oxygen must diffuse between the vascular and the surrounding tissues. Normal capillaries are relatively straight and well spaced, in contrast, the tumor vascular networks usually display more irregularity and the vessel wall shows higher permeability and less elasticity. The purpose of this study was to investigate the effect of capillary elasticity and tortuosity on the oxygen distribution and make further investigation on the mechanism of the formation of hypoxic regions in tumor. Method One-dimensional capillary model was coupled with the oxygen diffusion model. Oxygen transport was investigated in a Krogh and tortuous tissue model. The capillarygeometrywas obtained bythe one-dimensional model and transferred to the tissue model. Finite element method was employed in the analysis. Result The capillary radii along the flow direction under pressures were obtained for different initial radii and the oxygen distribution in the Krogh cylinder tissue model and the model with a tortuous capillary were computed. Conclusions when the capillary radius is small, the effect of vessel elasticity may have not significant effect on the oxygen distribution. However, with the capillary radius increasing, the effect on the oxygen transport becomes obvious. Moreover, with the tortuosity of the capillary increasing, the oxygen distribution becomes more heterogeneous, which is in agreement with the result in available reference. This work will be helpful to the investigation of oxygen transport within tumor.

Objective To establish a high performance liquid chromatography method for simultaneous determination of 10 kinds of phthalates in cosmetics. Methods 10 kinds of phthalates were separated on a C18 column using methanol-water as mobile phase at a flow rate of 1.0 ml/min, 25℃ column temperature and 280 nm detection wave. Results The detection limit of 10 kinds of phthalates were 0.1-7 mg/L. The precision was less than 3.95% and recovery rates varied from 98.59% to 108.07%. Conclusion The experimental results show that the method is simple, precise and accurate and suitable to simultaneous determination of 10 kinds of phthalates in cosmetics.

OBJECTIVE:To analyze the chemical components of the essential oil from Cnidium monnieri from Anhui versus Henan by GC-MS.METHODS:The essential oil was extracted from Cnidium monnieri by steam distillation;the chemical components of the essential oil were detected by GC-MS;the relative contents of the chemical components were computed using area normalization method.Peaks were separated by capillary GC-MS and their corresponding compounds were identified.RESULTS:36 chief components from Cnidium monnieri from Anhui and 45 from that from Henan were identified.28 components were noted in both.There was great difference between the two Cnidium monnieri.CONCLUSION:This study serves as a scientific basis for the further exploitation and utilization of Cnidium monnieri.

Objective To explore the operation methods,intraoperative treatment,measures for prevention and treatment of complications of laparoscopic cholecystectomy(LC) for acute cholecystitis.Methods Two hundred and serenty-two patients with acute cholecystitis from March 2003 to April 2007 were analyzed retrospectively.We performed total laparoscopic cholecystectomy in 260 cases and subtotal laparoscopic cholecystectomy with cholecystomucoclasis of remnant gallbladder in 12 cases.Results None of 272 cases was converted to open operation.There was no mortality,and no bile duct injury or major bleeding.Conclusions Laparoscopic cholecystectomy is a safe and effective method for acute cholecystitis and it has the advantages of minimal trauma,quick recovery and less blood loss.

Tremendous success has emerged in chimeric antigen receptor (CAR)-T cell therapy over the past few years, especially in leukemia and lymphoma. The first CAR-T cell product might be available in America in 2017 due to the emergence of the critical results. This paper focused on the key data presented at the 58th American Society of Hematology Annual Meeting.

Newborn due to low birth weight caused by preterm or fetal growth restriction will have an adverse effect on kidney development. In adulthood,the long-term adverse effects of low birth weight are associ-ated with a variety of kidney disease. Current studies suggest that low birth weight may participate in a variety of kidney disease development and progression by affecting the nephron number,the function of the vascular struc-ture and the endocrine level. This paper mainly reviews the relationship between low birth weight and kidney dis-ease.