Proteins, sugars and esterase isoenzymes of An. anthropophagus and An. sinensis were compared by IEF and two dimensional gel electrophoresis.The results show that there are some differences in the electrophoretic patterns between An. anthropophagus and An. sinensis. The glycoprotein, lipidprotein, glycolipi-dprotein, protein, polysaccharide and esterase isosnzymES showed 10, O, 6, 14, 2 and 13 bands in An. anthropophagus; 10, l, 5, 16, 3 and 15 in An. sinensis. There exist 234 and 240 polypeptide spots in An. anthropophagus and in An. sinensis, respectively, alto-gether 27.8% of polypeptide spots being different.

The current status and important advances in multi-disciplinary treatment for advanced gastric cancer,including surgery,chemotherapy and radiotherapy,were reviewed in this article.An operative scheme based on the stage and biologic behavior of the tumor is adopted with the D_2 procedure combined with intraoperative peritoneal hyperthermic chemotherapy(IPHC) for stage Ⅱ and Ⅲ patients.Accurate preoperative staging is of utmost importance in making an appropriate therapeutic strategy.For patients with stage Ⅲ_B and Ⅳ gastric cancer,neo-adjuvant chemotherapy followed by cyto-reductive surgery,is helpful to prolong survival and improve the quality of life.Besides the 5-FU,cisplatin and doxorubicin based regimen,combination regimens employing newer generation cytotoxic agents including oxaliplatin,irinotecan,taxanes provided more promising efficacy.Postoperative chemo-radiotherapy is presently under investigation.

Objective: To observe the clinical therapeutic efficacy of treatment of prolapse of lumbar intervertebral disc with lumber vertebral traction plus Ding's Rolling Technique.Methods: 133 cases of prolapse of lumbar intervertebral disc were randomly divided into two groups. There were 68 cases in the treatment group, which were treated with lumber vertebral traction plus Tuina. There were 65 cases in the control group, which were treated with simple lumber vertebral traction. Results: There were statistic differences in the two groups (x2=6.86,P＜0.01). The therapeutic efficacy in the treatment group was better than the control group.Conclusion: The clinical therapeutic efficacy treated with lumber vertebral traction plus Ding's Rolling Technique was better than simple lumber vertebral traction in treating prolapse of lumbar intervertebral disc.

Objective To investigate the effects of different concentrations of propofol on anoxia / reoxygenation injury to primary cultured rat hippocampal neurons. Methods Newborn (

Objective To investigate the protective effects of pharmacological preconditioning with diazoxide, an ATP-sensitive potassium channel opener, against anoxia/reoxygenation (A/R) injury to primarily cultured hippocampal neurons of newborn Wistar rats and the mechanism involved and the effects of diazoxide preconditioning on A/R-induced neuronal apoptosis. Methods Primarily cultured hippocampal neurons prepared by enzymatic digestion of hippocampus isolated from newborn (

Long non-coding RNAs (lncRNAs) are deifned as transcripts longer than 200 nt without coding capacities. Although they were initially argued to be transcriptional by-products of RNA polymeraseⅡ, recent evidence suggests that lncRNAs have been associated with a spectrum of biological processes, and aberrant lncRNA expression may be a major contributor to tumorigenesis, progression and prognosis. This study summarizes the up-to-date studies on lncRNAs in urological neoplasms.

Objective To investigate indications of thoracoscopic resection of esophageal carcinomas. Methods A total of 75 patients with esophageal carcinomas underwent video-assisted thoracoscopic surgery (VATS) from July 1997 to July 2003. The dissection of the esophagus and radical resection of lymph nodes were performed using a right-sided VATS approach. Then the stomach was dissociated through an upper-abdominal incision and was pulled up for esophago-gastric anastomosis in the left neck. Results A conversion to open surgery was required in 3 patients, in 2 of whom the tumors had enroded into the hilum and the posterior wall of the heart, and in 1 of whom the azygous vein was ruptured. The operation time was 160~220 min, with the intrathoracic operation time accounting for 60~90 min. The postoperative blood loss was 300~400 ml. No surgery-related deaths were noted. Postoperative complications included 1 case of volvulus of stomach, 2 cases of anastomotic leakage, 1 case of anastomotic stenosis, and 1 case of chylothorax. Follow-up checkups in 60 patients for 3 months ~ 7 years (mean, 4 years) found 2 cases of supraclavicular lymph node metastasis and 1 case of abdominal lymph node metastasis. The 1-, 3- and 5-year survival rates were 85.0% (51/60), 51.7% (31/60) and 40.0% (24/60), respectively. Conclusions Video-assisted thoracoscopic resection of esophageal carcinomas is feasibly suited to patients with localized lesions at phaseⅠ or Ⅱ.

Carcinoembryonic Antigen ; analysis ; Humans ; Luminescence

OBJECTIVE To discuss the occupational hazard factors and protection measures of Central Supply Unit.METHODS The occupational hazard factors in the Central Supply Unit was analyzed and some suggestion on the occupational protections to decrease occupational risk was done.RESULTS It is necessary to give some special training about the occupational safety,especially through various sharp instrument wound-treatments to establish some protection and countermeasures CONCLUSIONS The pertinent occupational protection strategy according to the actual hazard factors is more efficient and more benefit for the high quality management of Central Supply Unit which will enhance the standardization,quality and working efficiency,and ensure the work safety and quality.

Objective To investigate the efficacy and safety of glycoprotein Ⅱb/Ⅲa inhibitor (tirofiban) for elderly high-risk patients with acute coronary syndrome (ACS) undergoing percutaneous coronary interventional therapy (PCI). Methods Ninety four high-risk ACS patients admitted between Dec 2004 and Jul 2006 were randomly divided into the tirofiban group (n=46, mean age 68.5?11.4 years old) whom received tirofiban and half-dose of heparin, and the conventional interventional group (n=48, mean age 66.8?9.9 years old) whom were treated by full dose heparin before PCI. The coronary reperfusion flow (TIMI grades) of culprit vessel (CV) after PCI, cardiac enzyme changed 6 hrs and 12 hrs afterwards, major adverse cardiac events (MACE) rates within 30 days, bleeding and transfusion rates were analyzed and compared between the two groups. Results The average TIMI reperfusion grades (by frames) and CK-MB levels at 6 hrs and 12 hrs after PCI were better in the tirofiban group than in the conventional group (35.6?6.1, 16.2?3.9 mmol/L, 13.4?3.9 mmol/L vs 38.7?8.2, 18.4?4.8 mmol/L, 15.5?5.1 mmol/L, P