Objective To investigate the effects of different concentrations of propofol on anoxia / reoxygenation injury to primary cultured rat hippocampal neurons. Methods Newborn (

Objective To investigate the protective effects of pharmacological preconditioning with diazoxide, an ATP-sensitive potassium channel opener, against anoxia/reoxygenation (A/R) injury to primarily cultured hippocampal neurons of newborn Wistar rats and the mechanism involved and the effects of diazoxide preconditioning on A/R-induced neuronal apoptosis. Methods Primarily cultured hippocampal neurons prepared by enzymatic digestion of hippocampus isolated from newborn (

Objective: To observe the clinical therapeutic efficacy of treatment of prolapse of lumbar intervertebral disc with lumber vertebral traction plus Ding's Rolling Technique.Methods: 133 cases of prolapse of lumbar intervertebral disc were randomly divided into two groups. There were 68 cases in the treatment group, which were treated with lumber vertebral traction plus Tuina. There were 65 cases in the control group, which were treated with simple lumber vertebral traction. Results: There were statistic differences in the two groups (x2=6.86,P＜0.01). The therapeutic efficacy in the treatment group was better than the control group.Conclusion: The clinical therapeutic efficacy treated with lumber vertebral traction plus Ding's Rolling Technique was better than simple lumber vertebral traction in treating prolapse of lumbar intervertebral disc.

Long non-coding RNAs (lncRNAs) are deifned as transcripts longer than 200 nt without coding capacities. Although they were initially argued to be transcriptional by-products of RNA polymeraseⅡ, recent evidence suggests that lncRNAs have been associated with a spectrum of biological processes, and aberrant lncRNA expression may be a major contributor to tumorigenesis, progression and prognosis. This study summarizes the up-to-date studies on lncRNAs in urological neoplasms.

Proteins, sugars and esterase isoenzymes of An. anthropophagus and An. sinensis were compared by IEF and two dimensional gel electrophoresis.The results show that there are some differences in the electrophoretic patterns between An. anthropophagus and An. sinensis. The glycoprotein, lipidprotein, glycolipi-dprotein, protein, polysaccharide and esterase isosnzymES showed 10, O, 6, 14, 2 and 13 bands in An. anthropophagus; 10, l, 5, 16, 3 and 15 in An. sinensis. There exist 234 and 240 polypeptide spots in An. anthropophagus and in An. sinensis, respectively, alto-gether 27.8% of polypeptide spots being different.

The current status and important advances in multi-disciplinary treatment for advanced gastric cancer,including surgery,chemotherapy and radiotherapy,were reviewed in this article.An operative scheme based on the stage and biologic behavior of the tumor is adopted with the D_2 procedure combined with intraoperative peritoneal hyperthermic chemotherapy(IPHC) for stage Ⅱ and Ⅲ patients.Accurate preoperative staging is of utmost importance in making an appropriate therapeutic strategy.For patients with stage Ⅲ_B and Ⅳ gastric cancer,neo-adjuvant chemotherapy followed by cyto-reductive surgery,is helpful to prolong survival and improve the quality of life.Besides the 5-FU,cisplatin and doxorubicin based regimen,combination regimens employing newer generation cytotoxic agents including oxaliplatin,irinotecan,taxanes provided more promising efficacy.Postoperative chemo-radiotherapy is presently under investigation.

Objective To investigate indications of thoracoscopic resection of esophageal carcinomas. Methods A total of 75 patients with esophageal carcinomas underwent video-assisted thoracoscopic surgery (VATS) from July 1997 to July 2003. The dissection of the esophagus and radical resection of lymph nodes were performed using a right-sided VATS approach. Then the stomach was dissociated through an upper-abdominal incision and was pulled up for esophago-gastric anastomosis in the left neck. Results A conversion to open surgery was required in 3 patients, in 2 of whom the tumors had enroded into the hilum and the posterior wall of the heart, and in 1 of whom the azygous vein was ruptured. The operation time was 160~220 min, with the intrathoracic operation time accounting for 60~90 min. The postoperative blood loss was 300~400 ml. No surgery-related deaths were noted. Postoperative complications included 1 case of volvulus of stomach, 2 cases of anastomotic leakage, 1 case of anastomotic stenosis, and 1 case of chylothorax. Follow-up checkups in 60 patients for 3 months ~ 7 years (mean, 4 years) found 2 cases of supraclavicular lymph node metastasis and 1 case of abdominal lymph node metastasis. The 1-, 3- and 5-year survival rates were 85.0% (51/60), 51.7% (31/60) and 40.0% (24/60), respectively. Conclusions Video-assisted thoracoscopic resection of esophageal carcinomas is feasibly suited to patients with localized lesions at phaseⅠ or Ⅱ.

A series of multinuclear diethylenetriamine ligands were synthesized and used as artificial nuclease enzyme model. Target compounds were characterized by 1H NMR, 13C NMR, IR and ESI-MS. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes have also been performed and the results revealed that these complexes could act as powerful catalysts for the cleavage of pUC19 DNA after 48 h under physiological conditions. The hydrolytic cleavage mechanism of DNA plasmid by title compound was confirmed by T4 DNA ligase experiment.

Objective To investigate the protective effects of thalidomide on rats with acute necrotizing pancreatitis (ANP) and its mechanism. Methods Fifty four SD rats were randomly divided into the three groups; ANP group, thalidomide group and control group with 18 rats in each group. The model of ANP was induced by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct. Rats in thalidomide group received thalidomide 200 mg/kg body weight gastric lavage 1 h after ANP induction. The rats were sacrificed 3 h, 6 h, and 12 h after ANP induction, and the amount of intraperitoneal ascites was quantified. The serum levels of TNF-α, IL-6, IL-18 were measured by EUSA. The proportion of CD4 + T cell, CD8 + T cell in peripheral blood was determined by flow cytometry. The expression of TNF-α mRNA in pancreatic tissue were measured by RT-PCR. The expression of ICAM-1 protein in pancreatic tissue was measured by immunohistochemistry. Pancreatic tissue underwent pathologic examination. Results Six hours after surgery, the amount of ascite, serum levels of TNF-α, IL-6, IL-18, CD4 + T cell, CD,+ T cell, pancreatic TNF-α mRNA and ICAM-1 protein expression, pathologic score in control group was (1.03 ±0.31)ml,(57.17±11.29)pg/ml, (24.45 ±4.14)pg/ml, (64.23 ±21.85)pg/ml, (47.58 ±9.21)% , (40.88 ± 2.96)%, 0.07 ±0.02, 0.57 ±0.30, 0.67 ±0.81, respectively, and the corresponding values were (3.63 ± 0.38)ml, (107.54 ±33.05) pg/ml, (47.30 ± 11.40) pg/ml, (367.76 ± 108.43 ) pg/ml, (54.90 ± 7.15)%, (17.17 ±3.12)%, 0.65 ±0.26, 3.20 ±0.57, 11.50 ±1.87 in ANP group; and (1.45 ±0.53)ml, (80.60 ±20.48) pg/ml, (26.61 ±10.85) pg/ml, (321.82 ±85.20) pg/ml, (29.80 ±2.19)% , (15.52± 1.96)%, 0.35 ±0.23, 2.37 ±0.67, 8.00 ±3.03. Besides the value of CD8 + T cell was significantly decreased, all other values were significantly increased when compared with control group (P <0.05). Conclusions Thalidomide can decrease the release of inflammatory mediator, and reduce the pathological damage of pancreas of ANP rats by inhibiting TNF-α mRNA expression.

Varicose veins is the main clinical manifestations of the chronic venous insufficiency.TIPP transilluminated powered phlebectomy,a new minimally invasive surgery,received much attention while surgical techniques aimed at treating varicose vein were constantly updated.In this article,TIPP in the varicose vein surgery is reviewed from its history,operating techniques and advantages,and TIPP's future is being prospected.