1.Analysis on Application of Parenteral Nutrition in Our Hospital from Jan. to Jun. in 2008
China Pharmacy 1991;0(02):-
OBJECTIVE:To investigate the status quo and problems of application of parenteral nutrition in our hospital. METHODS:Cases of parenteral nutrition support(PN)from January to June in 2008 were reviewed and analyzed statistically. RESULTS:The problem of 637 patients treated with PN included indication,duration,prescription composition and incompatibility. CONCLUSION:The application of PN in our hospital is partly rational,nevertheless there are also some problems needed to be improved.
2.Clinical research on IL-2,IFN-γ,IL-10 and LDH in patients with acute leukemia
Lan ZHU ; Weihong SHEN ; Fang GONG
International Journal of Laboratory Medicine 2014;(19):2593-2594
Objective To study the levels of interleukin(IL)-2 ,interferon-γ(IFN-γ) and IL-10 in peripheral blood and the effec-tive concentration of lactate dehydrogenase(LDH) in serum of patients with acute leukemia .Methods 65 patients with acute leuke-mia were divided into non-remission group(n=35) and remission group(n=30) .50 healthy volunteers were used as control group . The levels of IL-2 ,IFN-γ ,IL-10 in peripheral blood were measured by enzyme linked immunosorbent assay(ELISA) .The effective concentration of LDH was measured by automatic biochemical analyzer .Results The levels of IL-2 ,IFN-γin non-remission group were significantly decreased compared with control group and remission group(P<0 .05) ,but the level of IL-10 in non-remission group was significantly increased compared with control group and remission group(P<0 .05) .The effective concentration of LDH in non-remission group was significantly higher than that in control group or remission group(P<0 .05) .Conclusion The levels of IL-2 ,IFN-γand IL-10 in peripheral blood and the effective concentration of LDH in serum of patients with acute leukemia have changed .Clinical detection of IL-2 ,IFN-γ,IL-10 and LDH may contribute to the early diagnosis of patients with acute leukemia .
3.Study of NO and FOXP3 in patients with pulmonary tuberculosis
Liyan SHEN ; Weihong SHEN ; Lan ZHU
International Journal of Laboratory Medicine 2015;(10):1364-1365
Objective To Study the expressions and significance of nitric oxide (NO) and forkhead box protein 3 (FOXP3) in patients with pulmonary tuberculosis .Methods Serum NO levels were measured by Griess colorimetric reaction .Flow cytometry was used to determine the number of CD4+CD25+ T cells in peripheral blood .The expression of FOXP3 mRNA was measured by real‐time polymerase chain reaction .Results Serum NO level in patients was (15 .71 ± 1 .26)μmol/L ,higher than the (5 .45 ± 0 .98)μmol/L of healthy controls (P<0 .05) .CD4+ CD25+ T cells comprised (4 .57 ± 0 .85)% of CD4+ T cells in patients ,higher than the (1 .83 ± 0 .49)% in healthy controls (P<0 .05) .CD4+ CD25+ T cells in the peripheral blood of patients with pulmonary tuberculosis highly expressed FOXP3 .Conclusion Patients with pulmonary tuberculosis could be with an increased level of NO and FOXP3 ,which might have important role in the pathogenesis of pulmonary tuberculosis .
4.Therapeutic Observation of Umbilicus Application withDa Huang (Rheum Officinale) during 5-7 a.m. for Senile Constipation after Hip Fracture
Lihua ZHU ; Hongmei XU ; Weihong QIAN
Shanghai Journal of Acupuncture and Moxibustion 2016;35(9):1081-1082
Objective To observe the clinical efficacy of umbilicus application withDa Huang (Rheum Officinale) during 5-7 a.m. in treating senile constipation after hip fracture.Method Fifty-nine elderly patients with constipation after hip fracture were randomized into a treatment group of 30 cases and a control group of 29 cases. The treatment group was intervened by umbilicus application withDa Huang (Rheum Officinale) during 5-7 a.m, while the control group was by umbilicus application withDa Huang (Rheum Officinale) at any time but avoiding 5-7 a.m. period. After a treatment course, the therapeutic efficacy was evaluated based on bowel movement frequency and shape of stool, and the two groups were compared with each other.Result The total effective rate and markedly-effective rate were respectively 93.3% and 70.0% in the treatment group versus 82.8% and 37.9% in the control group. The between-group difference in the total effective rate was statistically insignificant (P>0.05); there was a significant difference in comparing the markedly-effective rate between the two groups (P<0.01).Conclusion Umbilicus application withDa Huang (Rheum Officinale) during 5-7 a.m. is an effective method in treating senile constipation after hip fracture.
5.Resistance to erythromycin and resistance phenotypes of Streptococcus pneumoniae
Minggui WANG ; Demei ZHU ; Weihong WU
Chinese Journal of Infectious Diseases 2000;0(02):-
Objective To investigate bacterial resistance of Streptococcus pneumoniae against erythromycin and clindamycin, and resistance phenotypes of erythromycin resistant S. pneumoniae. Methods The MICs of erythromycin and clindamycin against 345 strains of S. pneumoniae were tested with agar dilution method, the phenotypes of erythromycin resistant S. pneumoniae were detected by double disc test. Results The resistance rates of S. pneumoniae to erythromycin and clindamycin were 53.0%(183/345) and 49.6%(171/345), respectively. Among erythromycin resistant S. pneumoniae , the percentages of cMLS, iMLS and M phenotype were 90.3% (159/176), 5.7% (10/176) and 4.0%(7/176), respectively. Conclusions The resistance rate of S.pneumoniae against erythromycin is very high in Shanghai. The main phenotype is cMLS.
6.Blood purification therapy of gestational hyperlipidemic pancreatitis:A case report
Hongliang LI ; Weihong AN ; Yangyu ZHAO ; Xi ZHU
Journal of Peking University(Health Sciences) 2003;0(05):-
Gestational hyperlipidemic pancreatitis is an uncommon complication of pregnancy that incurs a high risk of morbidity and mortality of both maternal and fetal patients.We described the response of continous renal replacement therapy in a woman with extreme gestational hyperlipidemia and severe pancreatitis.Five consecutive plasma exchanges didn't lead to a remarkable reduction(10.4% and 4.8%,for the first and fourth times,respectively) at triglyceride levels as the literatures have reported,which reveals that plasma exchange and continous veno-venous heamofiltration may be importment,instead of crucial methods in dealing with gestational hyperlipidemic pancreatitis.Abstract:SUMM ARY Gestational hyperlipidem ic pancreatitis is an uncommon complication of pregnancy that in-curs a high risk ofmorbid ity and mortality of both maternal and fetal patients.W e described the response of continous renal replacement therapy in a woman with extreme gestational hyperlipidem ia and severe pancreatitis.Five consecutive plasma exchanges d idn’t lead to a remarkable reduction(10.4% and 4.8%,for the first and fourth times,respectively) at triglyceride levels as the literatures have reported,which reveals that plasma exchange and continous veno-venous heamofiltration may be importment,in-stead of crucialmethods in dealing with gestational hyperlipidem ic pancreatitis.
7.Post-operative ipsilateral occipital hematoma in a patient with mesial temporal lobe epilepsy: A case report
Lichao Sun ; Yang Lv ; Zhanpeng Zhu ; Weihong Lin ; Jiqing Qiu ; Weihong Lin
Neurology Asia 2016;21(2):177-180
We report a case of a 35-year-old right-handed male patient with drug-resistant mesial temporal lobe
epilepsy who developed ipsilateral occipital hematoma after right temporal anterior lobectomy and
amygdalohippocampectomy. Patient did not report taking any drugs with anticoagulant effect, such
as aspirin or valproate. There was no past history of hypertension and the pre-operation tests were
normal. Pre-operative brain magnetic resonance imaging revealed no abnormality in the right occipital
lobe. Overdraining of cerebrospinal fluid may have triggered this remote cerebellar hemorrhage.
8.Liver function changes in rheumatoid arthritis patients treated with tofacitinib: systematic review
Jiaqi LI ; Weihong GE ; Huaijun ZHU ; Zena CHANG ; Zhou HAN
Chinese Journal of Rheumatology 2017;21(6):392-397,后插2
Objective To evaluate the effects of tofacitinib on liver function in rheumatoid arthritis (RA) patients.Methods Literature search was performed in databases including PubMed,Cochrane Library,China National Knowledge Infrastructure and Wanfang to identify randomized controlled trials about RA treated with tofacitinib.The retrieval time was up to August 2016.Meta-analysis was conducted by Revman 5.5 software.Results A total of 7 studies were included,involving 2 965 patients.The results of Meta-analysis revealed that the incidence of alanine transaminase (ALT)>1 upper limit of normal (ULN) in patients receiving both 5 mg and 10 mg bid tofacitinib was significantly higher than placebo [5 mg bid tofacitinib:RR=1.48,95%CI (1.20,1.82),P=0.000 2;10 mg bid tofacitinib:RR=1.67,95%CI (1.37,2.05),P<0.01];there was no significant difference in the incidence of ALT>3 ULN [5 mg bid tofacitinib:RR=1.81,95%CI (0.57,5.79),P=0.32;10 mg bid tofacitinib:RR=1.36,95%CI (0.57,5.25),P=0.49];the incidence of aspartate transaminase (AST)>1 ULN was significantly higher than placebo [5 mg bid tofacitinib:RR=1.59,95%CI (1.25,2.03),P=0.000 2;10 mg bid tofacitinib:RR=1.90,95%CI(1.50,2.40),P<0.01],there was no significant difference in the incidence of AST>3 ULN [5 mg bid tofacitinib:RR=1.17,95%CI (0.27,5.17),P=0.83;10 mg bid tofacitinib:RR=0.95,95%CI (0.26,3.44),P=0.94].Conclusion Tofacitinib slightly increases ALT and AST in patient with RA.Due to the limited sources and lack of domestic studies,more randomized controlled trials are still needed to verify the above conclusion.
9.Influence of Calcineurin Gene Polymorphisms on the Efficacy of Cyclosporine A
Jing YAO ; Yingmin HAI ; Dingding CHEN ; Weihong GE ; Huaijun ZHU
China Pharmacy 2016;27(29):4033-4036,4037
OBJECTIVE:To study the influence of calcineurin gene polymorphism on the efficacy of cyclosporine A (CsA). METHODS:The blood samples of patients treated with CsA were collected. The trough blood concentration of CsA was detected by EMIT. The genotype of PPP3CA and PPP3CB was assayed by RFLP-PCR method. The expression of NFAT-regulated gene IL-2, IFN-γand GM-CSF were measured by RT-qPCR,which were used to define the index of indirect efficacy of CsA. The relationship of gene polymorphism with CsA efficacy was study by relationship analysis and multiple factor regression method,etc. RESULTS:A to-tal of 100 blood samples were collected. There was no significant correlation between the expression of CsA efficacy-related NFAT-reg-ulated gene GM-CSF and trough concentration of CsA(rGM-CSF=-0.04,P=0.238);the expression of IL-2 and IFN-γ were negatively correlated with trough concentration of CsA significantly(rIL-2=-0.384 3,P<0.001;rIFN-γ=-0.335 4,P<0.001). Using the average value of mRNA expression of IL-2 and IFN-γas indirect efficacy index,the polymorphism of PPP3CB rs3763679 site significantly in-fluenced the efficacy of CsA(P<0.05),but PPP3CA rs3804358 had no any effect on it(P>0.05). Stratified analysis showed that among patients with immune disease underwent renal transplantation,efficacy of patients with PPP3CB rs3763679 genovariation(TC+TT) were better than those with wild-type gene (CC)(P<0.05). After the efficacy was normalized by CsA trough concentration, multivariate analysis showed that normalized efficacy of CsA was negatively correlated with gender,PPP3CB rs3763679,lactate dehy-drogenase and creatinine significantly,but positively correlated with PPP3CA rs3804358,leucocyte count,usea nitrogen,glycerin trilaurate,etc. CONCLUSIONS:PPP3CB rs3763679 gene polymorphism influence the efficacy of CsA;among patients with immune disease underwent renal transplantation,efficacy of patients with PPP3CB rs3763679 TT+TC is better than that of CC type. At the same time,gender,PPP3CA rs3804358,leucocyte count,usea nitrogen,glycerin trilaurate and other factors all can influence the nor-malized efficacy of CsA to different extent. Multiple factors should be considered when using CsA.
10.The relationship between acquired carbapenemases and resistance of gram-negative bacill
Jilu SHEN ; Demei ZHU ; Weihong WU ; Xiaogang XU ; Minggui WANG
Chinese Journal of Laboratory Medicine 2008;31(4):408-414
Objective To investigate acquired carbapenemases and prevalence of carbapenem- resistant gram-negative bacill.Methods The antimicrobial susceptibility was determined by agar dilution method.Metallo-B-lactamase(MBLs)were screened by EDTA-disk synergy tesL The encoding genes of MBLs were amplified by PCR followed by sequencing.Strain homology Was investigated by pulsed-field gel electronphoresis(PFGE).Results In 141 carbapenem-resistant Pseudomonas aeruginosa(P.aeruginosa), there were three resistant patterns which were imipenem(IMP)-resistant+meropenem(MEM)-resistant (66.7%),IMP-resistant+MEM-sensitive(32.6%),and IMP-resistant+MEM-sensitive(0.7%).AⅡthe carbapenem-resistan Acinetobacter baumannii(A.baumannii),Acinetobacter lwoffi(A.lwoffi),Citrobactor freundii(C.freundii),Klebsielta pneumoniac(K.pneumoniac)and Serratia were resistant to imipenem and meropenem.Four strains of 141 P.aeruginosa were positive by EDTA-disk synergy test,and they produced VIM-2-type Metallo-B-1actamase.Of 34 carbapenem-resistan A.baumannii,30 strains produced OXA-tllrpe.Among them, OXA23, OXA24 and OXA66 accounted for 79.4%,38.2% and 67.6%,respectively.And 22 of 34 strains(64.7%)produced multiple OXA-carbapenemases.All 7 strains A.lwoffi produced OXA-23-type carbapenemases.A11 11 strains C.freundii,5 strains k pneumoniac and 1 strains Serratia produced KPC-2-type carbapencmases.And 6 of 11 strains C.freundii produced new subtype IMP-8.Of 15 PFGE type in 34 strains A.baumannii,14 strains belonged to A-type,7 strains belonged to B-type.Seven A.lwoffi strains distilbuted in difierent PFGE type.Four strains of P.aeruginosa producing VIM-2.type Metallo-8-lactamase did not have the same PFGE type.Eleven C.freundii strains had the same PFGE type.Five k pneumoniae strains had the sanle PFGE type.Conclusions Drug resistance to 12 common antibiotics in carbapenem-resistant gram-negative bacill was higher than non-carbapenem-resistant gram-negative.The former produced many kinds of carbaponemases and the strains prducing carbapenemases were prevalent in the C.freundii, A.baumanii, and k pneumoniae.