Objective To construct 7 shRNA plasmids targeted to Gcn5 and to explore their role on histone acetylation in regulating MSCs differentiation into cardiomyocytes.Methods MSCs were isolated and passaged.Seven shRNA plasmids were constructed by pGenesil-1(4.9 kb) vector with GFP gene.MSCs were treated with 10 ?mol/L 5-azacytidine respectively for 24 h,2,4 and 7 d and transfected with the plasmids via lipofectamine~(TM)2000.Gcn5,as the marker of the acetylation,and GATA4,as the marker of cardiomyocytes,were detected in MSCs during differentiation with or without shRNA plasmids transfection by RT-PCR.Results MSCs were fibroblast-like but changed morphologically after induction.Seven Gcn5 shRNA plasmids were constructed and identified by restriction endonucleases PstⅠ/SalⅠand DNA sequencing.The expression of Gcn5 and GATA4 could be detected in MSCs without shRNA plasmids tranfection,but no expression after shRNA plasmids transfection.Conclusion Gcn5 plays a very important role in MSCs differentiation into cardiomyocytes,which lays the basis for further research of the importance of acetylation in the regulation of cell differentiation.

It is a mature technology producing single-use syringes by polypropylene(PP). But the traditional sterilization process for single-use syringe pollutes the environment, and the residues endanger the health of users. Irradiation sterilization, instead of traditional sterilization process, is the developing trend. However, the traditional PP does not have the ability of radiation resistance. These methods can improve the radiation resistance ability of PP effectively by adding the stabilizer or floating agent. Toughening or temperature treatment can have the same function. Radiation-resistant PP is introduced in this paper. Furthermore, the developing trends for the materials are discussed.

? AIM: To investigate the immunosuppression of artemisinin in the early-phase response( EPR) of allergic conjunctivitis in a murine model induced by pollen.?METHODS:Twenty-five Balb/c mice were divided into five groups at random: A: model group, B: artemisinin topical treatment group, C: oral artemisinin group, D:dexamethasone topical treatment group, E: negative control group. Balb/c mice were first sensitized with mixture of ragweed crude extract and complete Freund’s adjuvant by left footpad and root of tails injection at days 0. At days 7 and 14, mice were strengthen sensitized by intraperitoneal injection. Negative control group replacd with a mixture of equal amounts of Freund’s adjuvant and PBS mixture. Treatment time was between 21-27d:group B received 1% topical artemisinin eye drops 4 times per day; group C received artemisinin at doses of 600mg/kg orally once per day; group D received topical dexamethasone eye drops 4 times per day. The model and naive groups replaced with solvent for control. After treatment, mice were excited by ragweed pollen crude infusion drops. Within 1h after the excitation, the eyes were taken for histopathology testing, the serum for detection of specific IgE for ragweed pollen.?RESULTS:After excitation, allergy symptoms of model group were more significantly compared with negative control group and the treatment groups; allergy symptom score, mast cell degranulation ratios and the ragweed pollen specific IgE in serum in the topical treatment groups and oral group were higher than that in the negative control group, but significantly lower than that in model group.?CONCLUSION: Artemisinin topical treatment and oral treatment for mice could inhibit the symptoms of pollen allergic conjunctivitis, early-phase response of mast cell degranulation ratio and specific IgE generation for ragweed pollen, suggesting that artemisinin has some therapeutic effect for pollen allergic conjunctivitis.

The imported schistosomiasis cases in the Chinese literature and reports from the infectious disease monitoring in-formation system from 1979 to 2013 were collected and compiled. Totally 365 cases were reported to be infected with outside schis-tosomiasis, including 239 cases of schistosomiasis haematobia (74.0%) and 71 cases of schistosomiasis mansoni (22.0%), and 42 foreigners (11.5%) and 323 Chinese (88.5%). The infection areas involved 15 countries and regions in Africa. Totally 74.92%of pa-tients worked in the construction or geological prospecting in the wild field. According to the analysis of these cases, we found that the management system of prevention and control of imported schistosomiasis cases was not perfect including the high missing re-port rate, high misdiagnosis rate, and no standard diagnostic criteria. We suggest that all the levels of CDC or health administrative authorities should adopt the following relevant control measures to strengthen the imported schistosomiasis prevention and control in order to reduce the damage to the public health and the risk of the spread of African schistosomiasis in China:1. to establish and perfect the imported schistosomiasis monitoring and control system in China;2. to integrate the dynamic information platform of la-bor export and establish the comprehensive prevention and control management system of infectious diseases;3. to standardize the diagnosis and treatment of oversea imported schistosomiasis; 4. to strengthen the research on the transmission risk of imported schistosomiasis in the territory of China.

Objective To study the antagonism of water-soluble compounds of salvia-salvianolic acid A(SalA),salvianolic acid B(SalB) and catechu aldehyde(CA) to Cr(Ⅵ) induced genetic toxicity in Vicia faba root tip cells.Methods The root tip of Vicia faba were treated with SalA,SalB,CA(5-1000 ?g/ml) and every compound combined with 100 ?g/ml Cr(Ⅵ) respectively,and with distilled water as control group.The micronucleus rate(MNR),the mitotic index(MI) and the chromosome aberration rate(CAR) of Vicia faba root tip cells were observed by micronucleus assay and chromosome aberration assay.Results Compared with control group,treatments of 50,100 ?g/ml SalA,50 ?g/ml SalB,100,500 ?g/ml CA significantly increased MI(P

Paraneoplastic pemphigus (PNP) is an autoimmune mucocutaneous disease first named by Anhalt, et al. in 1990. The disease is characterized by such distinctive clinical symptoms and signs as severe, painful mucosal erosions, polymorphous skin lesions, histopathology hallmarks, and immunological findings. The situation typically presents in patients with lymphoproliferative diseases and primarily malignancies. A main challenge of the study is the relationship between the existence of associated tumors and the autoimmune reaction to the skin. Some researchers suspected that the possible expression of foreign antigens on the tumor can cross react with epidermal antigens inducing the auto-reactive clones of T-lymphocytes. Some speculated that the type of tumors associated with PNP may produce plakin proteins that result in initiation of the immune response. Other reports believed the autoimmune reaction is related to the epitope spreading or to the changing of cytokines. We analyzed 12 PNP patients diagnosed in our department in the past few years. An intensive study to the B cells in the PNP associated with tumors demonstrated that the tumors have structural basis to produce antibody. The similar immunoglobulin heavy chain genes of tumor B-cells in 7 patients strongly suggested that the B cell clones were functional and recognized the same antigen epitope. The autoantibodies secreted by the tumor can react against specific plakin proteins in epidermis, lead to the impairment of cell-cell adhesion, and cause the mucocutanous lesions. The clinical significance of the results indicates the importances of early finding and total resection of the associated tumors, and the usage of IVIG pre or during operation to prevent Bronchitis Obliterans. The new finding is also important for the study of other antibody mediated autoimmune diseases.

High mobility group box-B1 (HMGB1)is a non histone DNA-binding protein and extracellular molecule related to damage.Normally it is located inside the cell and exerts a variety of biological effects. It has been demonstrated that the expression of HMGB1 presents in almost all gastrointestinal tumors and is closely correlated with the cell growth, proliferation, invasion and metastasis of gastrointestinal tumors. It has been discovered that HMGB1 inhibitor inhibits growth and metastasis of gastrointestinal tumors, which might become a new approach to the treatment of gastrointestinal tumors.

Objective:To observe the fluoride release rate of F-containing thermoplastic resina,trying to find a suitable concentration of fluoride in thermoplastic resina to prevent caries in base teeth of partial denture restorations.Methods:Na2(PO3)F-containing thermoplastic resina,respectively 0,10%,15%,20% and 25%(w/w)in concentration were cut into three 1 g blocks and immersed in 100,200 and 500 ml deionized water.These composite resins in 100 ml and 200 ml solutions were replaced every 1,2,3,6,9,16 days(every 7 days after that)until two months later,500 ml solution was reserved after 3,6,9 days every 7 days later until two months,2 ml of which were reserved every time.The fluoride release rates of these thermoplastic resina were tested by potentiometry.Results:All thermoplastic resina containing different concentrations of fluoride could release F-during the period of 65 days.Conclusion:When Na2(PO3)F is mixed in thermoplastic resina,it releases fluoride slowly.Thermoplastic resina containing 15% F-has higher release rates of fluoride.

AIM: To investigate the effect and mechanism of Mingjing decoction combining with argon laser on the levels of plasma vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy(DR).
METHODS:The 66 patients(118 eyes)with DR at Ⅲ ~ Ⅴstage were randomly divided into two groups (treatment group and control group). The control group (33 patients with 56 eyes ) was treated with argon laser, and the treatment group (33 patients with 62 eyes) was treated with mingjing decoction combining with argon laser, and a blank group(30 eyes) was set up. The levels of plasma VEGF were detected by enzyme linked immunosorbent assay (ELISA). The results of best corrected visual acuity and fundus fluorescein angiography(FFA) were analyzed, as well as detected glycosylated hemoglobin ( HbA1c), blood coagulation function and the function of liver and kidney.
RESULTS: Patients with DR had significantly risen plasma VEGF before treatment when compared with blank control group ( P < 0. 05 ). After 3mo of combined treatment, the levels of plasma VEGF significantly reduced and HbA1c decreased in patients treated with mingjing decoction and argon laser, there were statistically significant difference compared to control group ( P < 0. 05 ). In the treatment group, the best corrected visual acuity and FFA were significantly improved in patients treated with mingjing decoction combining with argon laser compared to patients treated with argon laser alone after 3mo of combined treatment. CONCLUSION: Mingjing decoction combining with argon laser for DR can effectively reduce the level of plasma VEGF, stabilize blood sugar levels, improve the function of retina, and delay DR progresses.

Despite the continuous improvement of cardiopulmonary bypass(CPB),renal dysfunction remains a frequent complication of cardiac surgery.Previous studies reported that the incidence of acute renal dysfunction following CPB was 7%-40%,while that of acute renal failure(ARF) was 1%-10%.In those who developed ARF,the prognosis was poor,with mortality of about 40%-80%.The present paper reviews the mechanism,risks and available preventive measures of acute renal injury following CPB.