It is a mature technology producing single-use syringes by polypropylene(PP). But the traditional sterilization process for single-use syringe pollutes the environment, and the residues endanger the health of users. Irradiation sterilization, instead of traditional sterilization process, is the developing trend. However, the traditional PP does not have the ability of radiation resistance. These methods can improve the radiation resistance ability of PP effectively by adding the stabilizer or floating agent. Toughening or temperature treatment can have the same function. Radiation-resistant PP is introduced in this paper. Furthermore, the developing trends for the materials are discussed.

Objective To construct 7 shRNA plasmids targeted to Gcn5 and to explore their role on histone acetylation in regulating MSCs differentiation into cardiomyocytes.Methods MSCs were isolated and passaged.Seven shRNA plasmids were constructed by pGenesil-1(4.9 kb) vector with GFP gene.MSCs were treated with 10 ?mol/L 5-azacytidine respectively for 24 h,2,4 and 7 d and transfected with the plasmids via lipofectamine~(TM)2000.Gcn5,as the marker of the acetylation,and GATA4,as the marker of cardiomyocytes,were detected in MSCs during differentiation with or without shRNA plasmids transfection by RT-PCR.Results MSCs were fibroblast-like but changed morphologically after induction.Seven Gcn5 shRNA plasmids were constructed and identified by restriction endonucleases PstⅠ/SalⅠand DNA sequencing.The expression of Gcn5 and GATA4 could be detected in MSCs without shRNA plasmids tranfection,but no expression after shRNA plasmids transfection.Conclusion Gcn5 plays a very important role in MSCs differentiation into cardiomyocytes,which lays the basis for further research of the importance of acetylation in the regulation of cell differentiation.

? AIM: To investigate the immunosuppression of artemisinin in the early-phase response( EPR) of allergic conjunctivitis in a murine model induced by pollen.?METHODS:Twenty-five Balb/c mice were divided into five groups at random: A: model group, B: artemisinin topical treatment group, C: oral artemisinin group, D:dexamethasone topical treatment group, E: negative control group. Balb/c mice were first sensitized with mixture of ragweed crude extract and complete Freund’s adjuvant by left footpad and root of tails injection at days 0. At days 7 and 14, mice were strengthen sensitized by intraperitoneal injection. Negative control group replacd with a mixture of equal amounts of Freund’s adjuvant and PBS mixture. Treatment time was between 21-27d:group B received 1% topical artemisinin eye drops 4 times per day; group C received artemisinin at doses of 600mg/kg orally once per day; group D received topical dexamethasone eye drops 4 times per day. The model and naive groups replaced with solvent for control. After treatment, mice were excited by ragweed pollen crude infusion drops. Within 1h after the excitation, the eyes were taken for histopathology testing, the serum for detection of specific IgE for ragweed pollen.?RESULTS:After excitation, allergy symptoms of model group were more significantly compared with negative control group and the treatment groups; allergy symptom score, mast cell degranulation ratios and the ragweed pollen specific IgE in serum in the topical treatment groups and oral group were higher than that in the negative control group, but significantly lower than that in model group.?CONCLUSION: Artemisinin topical treatment and oral treatment for mice could inhibit the symptoms of pollen allergic conjunctivitis, early-phase response of mast cell degranulation ratio and specific IgE generation for ragweed pollen, suggesting that artemisinin has some therapeutic effect for pollen allergic conjunctivitis.

Despite the continuous improvement of cardiopulmonary bypass(CPB),renal dysfunction remains a frequent complication of cardiac surgery.Previous studies reported that the incidence of acute renal dysfunction following CPB was 7%-40%,while that of acute renal failure(ARF) was 1%-10%.In those who developed ARF,the prognosis was poor,with mortality of about 40%-80%.The present paper reviews the mechanism,risks and available preventive measures of acute renal injury following CPB.

Synthetic agonists of peroxisome proliferator-activated receptors-?(PPAR?) have an important role in the preclinical models of metabolic diseases.In the recent years,it has been demonstrated that PPAR? agonists can normalize blood lipids and reduce insulin resistance and adiposity in rodents and primates.PPAR? activation in the skeletal muscle regulates the oxidative capacity of the mitochondrial apparatus,switches fuel preference from glucose to fatty acids and reduces triacylglycerol storage.PPAR? agonists also reduce insulin levels in serum,increase the sensitivity of insulin and improve glucose-tolerance.The genetic polymorphism in PPAR? has been shown to be associated with blood lipids,obesity and type 2 diabetes.

Objective To observe the evaluation of multi‐tumor marker protein chip in breast cancer with neoadjuvant chemo‐therapy ,and to find high saitivity tumor makers ,then evaluate the clinical significance .Methods Forty health examination persons (control group) and sixty‐four breast cancer patients(experimental group) were accepted with multi‐tumor marker protein chip in‐spection ,then we evaluated the markers of diagnosis .The experimental group patients were accepted four cycles preoperative chem‐otherapy with TAC plan ,and tested multi‐tumor marker protein chip three weeks after therapy .Then we evaluated the clinical sig‐nificance of the markers above‐mentioned .Results The positive rate of CA153 ,CA125 ,CEA and FT was 32% ,27% ,21% and 19% in the experimental group ,it indicated significant change compared with the control group (P<0 .05) .And it indicated signifi‐cant reducing change of CA153 ,CA125 and CEA in the experimental group (P<0 .05) after therapy .The sensitivity would be in‐creased when the four markers were tested combined .Conclusion CA153 ,CA125 ,CEA and FT could be used as assistance diagno‐sis index ;CA153 ,CA125and CEA could keep consistence to the curative effect of neoadjuvant chemotherapy and have clinical value .

Objective To screen the optimized processing technology of wine radix Scutellariae .Methods With the the com‐prehensive score of the yield rates of baicalin ,wogonoside and baicalein as the index ,the orthogonal design was used to investigate the mass fractions of yellow wine quantity ,stir‐frying temperature and frying time of wine radix Scutellariae .Results The optimum yellow wine mass fraction was 10% ,the stir‐frying temperature was 120℃ ,the frying time was 6 min ,and this processing technolo‐gy was stable and feasible .Conclusion The orthogonal design can be used for the optimization of processing technology of wine ra‐dix Scutellariae .

Objective　To evaluate the effects of the excimer laser in situ keratomileusis (LASIK) for treatment of high myopia.Methods　LASIK was performed on 308 eyes of 186 patients who were followed up for one year. The patients were divided into two groups (group A and group B) according to preoperative diopters.Results　One year after surgery, in group A(-6.0～-10.0D) the uncorrected visual acuities of 0.5 and 1.0 were in 95.76% and 77.58% of the eyes, the mean residual refraction was -0.32D±0.46D. In group B(-10.25～-20.0D) the uncorrected visual acuities of 0.5 and 1.0 were in 62.24% and 37.06% of the eyes, the mean residual refraction was -0.92D±1.13D.Conclusion　It is an effective and safe refraction surgery to correct high myopia with LASIK.

High mobility group box-B1 (HMGB1)is a non histone DNA-binding protein and extracellular molecule related to damage.Normally it is located inside the cell and exerts a variety of biological effects. It has been demonstrated that the expression of HMGB1 presents in almost all gastrointestinal tumors and is closely correlated with the cell growth, proliferation, invasion and metastasis of gastrointestinal tumors. It has been discovered that HMGB1 inhibitor inhibits growth and metastasis of gastrointestinal tumors, which might become a new approach to the treatment of gastrointestinal tumors.

Objective:To observe the fluoride release rate of F-containing thermoplastic resina,trying to find a suitable concentration of fluoride in thermoplastic resina to prevent caries in base teeth of partial denture restorations.Methods:Na2(PO3)F-containing thermoplastic resina,respectively 0,10%,15%,20% and 25%(w/w)in concentration were cut into three 1 g blocks and immersed in 100,200 and 500 ml deionized water.These composite resins in 100 ml and 200 ml solutions were replaced every 1,2,3,6,9,16 days(every 7 days after that)until two months later,500 ml solution was reserved after 3,6,9 days every 7 days later until two months,2 ml of which were reserved every time.The fluoride release rates of these thermoplastic resina were tested by potentiometry.Results:All thermoplastic resina containing different concentrations of fluoride could release F-during the period of 65 days.Conclusion:When Na2(PO3)F is mixed in thermoplastic resina,it releases fluoride slowly.Thermoplastic resina containing 15% F-has higher release rates of fluoride.