Objective To evaluate the standardized general practice training program.Methods A questionnaire survey was conducted among general practitioners who completed the standardized residency training program in Zhongshan Hospital,Fudan University from January 2000 to June 2011.Results During this period 183 residents received general practice training,15 were excluded the survey due to loss of follow-up or other reasons,168 questionnaires were distributed and 165 valid questionnaires were returned with a recovery rate of 98％.The main findings:2/3 of surveyed GPs were sent by their affiliated institutions; 85％ evaluated the program as highly or very highly; 56％ thought that their expectations were completely or mostly fulfilled; 68％ thought that the program enhanced their clinical capability and theoretical levels; over half thought that the clinical rotation and strict teaching management were the advantages of the program; 84％ thought that they improved their skills,knowledge and ideas; 74％ thought that the training program was very good or fairly good; 48％ thought that the preceptors in communities were comparatively weak and some theoretical lessons were not relevant to the context of the community.The surveyed GPs required improving of practice components,shortening the total length of training and increasing the teaching contents on scientific research.Conclusion The surveyed general practitioners give credit to the GP residency training in Zhongshan Hospital and put forward some proposals for further improvement.

Gene therapy of spinal cord injury (SCI) is the most promising method compared with the others, because it doesn't involve the problems of resource and higher exclusion which respectively exists in fetal nerve transplantation and peripheral nerve transplantation. There are two ways of gene therapy to be chosen: one is to transfer objective genes to the target-cells in vivo directly; the other is to transfer objective genes to one proper kind of transplantable cells firstly, then graft the highest expressing cells to the target-cells in vivo. To realize the transfer of genes to cells, two measures are used in common: physical or chemical measure such as micro infection et al and biochemical measure i. e. gene modified defective virus. Although there are some questions unresolved in this field, the clinical value of gene therapy of SCI in the future is depended.

Objective:To investigate the anatomy of mandibular incisors using microcomputed tomography(Micro-CT).Methods:62 extracted mandibular incisors were selected from dental clinic and observed by Micro-CT.The anatomy of the teeth was analyzed. Results:83.9% of mandibular incisors had a single canal,72.6% had no accessory canal.The location of the apical foramen varied considerably.The mean distance from the root apex to the major lateral apical foramen was (0.613 ±0.298)mm.Of the canals the bucco-lingual diameter were greater than the mesio-distal diameters and the root bucco-lingual canal tapers were greater than the me-sio-distal.Conclusion:The morphology data of mandibular incisors were obtained using Micro-CT.

Objective To explore the diagnostic value of multi-slice spiral CT MIP-MPR image in regional lymph node metastasis of colonic carcinoma.Methods CT findings of 33 cases with pathological confirmed colonic carcinoma were analyzed retrospectively . All patients were underwent three-phase 256-slice CT dynamic enhancement scanning.All data were processed in workstation,to obtain routine MPR and MIP-MPR image.The regional lymph nodes of colon cancer were counted,and divided into 3 groups accord-ing to the short diameter of lymph node:group I (3.0-5.0 mm),group Ⅱ(5.1-10 mm),and group Ⅲ(10.1 mm up).The diag-nostic sensitivity of CT MPR and MIP-MPR image in checking out lymph nodes of different groups was compared by paired test.Re-sults The average value(x ±s )of lymph node number of three groups with routine MPR and MIP-MPR image were:group I, 3.66±1.99,6.09±2.44 (t=1 1.6,P <0.05);group Ⅱ,3.72±1.79,4.03±1.89 (t=3.73,P <0.05);group Ⅲ,1.21±1.31, 1.27±1.46(t=1.44,P >0.05).There were statistical differences in group I and group Ⅱ.Sensitivity of MIP-MPR image in chec-king out regional small and middle lymph nodes of colonic carcinoma was higher than routine MPR image.Conclusion Multi-slice spiral CT MIP-MPR image is a good way to checking out regional small and middle lymph nodes of colonic carcinoma.

Hepatocellular carcinoma (HCC)is a common malignant tumor in the world,and surgical resection and liver transplantation are two radical treatment modalities,but only 10%-20%of all patients can receive such treatments.In recent years,local therapies including radiofrequency ablation,microwave ablation,cryoablation,and the irreversible electroporation ablation which appeared recently have gradu-ally become the alternative therapies for the patients who are unable to undergo surgery.In addition to local tumor growth control and im-provement in survival outcomes,the ablation technology also helps to downgrade tumor for secondary resection.This article focuses on the re-search progress in radiofrequency ablation alone and in combination with other therapies in the treatment of HCC,compares radiofrequency ablation with other local ablative therapies,and briefly introduces the application of intelligent navigation technology in adjuvant ablation. With the development of medical imaging and progress in related fields,the ablation technology will be widely applied in clinical practice in the future.

This experiment was made on 4-6 week-old nude mice(NM). The CNE-2 cells were inoculated subcutaneously into each side of the neckback for the original generation. Implanted fresh transplanted NPC tissue to NM's neck-back with cannula for next passage. We passed up to 8 generations and the transplantability was 100%. We observed dynamicrly the changes of NM's immune status after they have bearing the NPC as following: (1) The net weight was significantly reduced. (2) Splenic index(S. I.) and tumor weight(T. W.) increased. S. I. was highly related to T. W. (r=0.8395). (3) Splenic NK activity slightly increased in 5 days, began to decrease in 10 days and significantly decreased in 15 or more days. NK activity was negative related to T. W. and S. I. markedly (r=-0.7132, r=-0.8237). (4) Splenic NK cells had excellent responsiveness to human IFN in vitro in 5 days and still had some in 10 days, but it had not any after 15 days. (5) Natural proliferative ratio of tumor bearing NM's splenic cells was higher than that of control group, but the responsiveness to ConA was much lower. (6) Ai Ke Xing suppressed the growth of transplanted NPC by means of decreasing sera MDA enhancing host's splenic NK activity and increasing mononuclear infiltration of the transplanted NPC tissue. So we concluded that in NM whose T cells were deficient splenic NK cells were important immunocompetent cells for resisting the growth of transplanted NPC.

Objective To investigate the role of IP-10 in the pathogenesis of ankylosing spondylitis (AS). Methods The peripheral blood monocytic cells (PBMC) were collected from 39 AS patients, and the mRNA expression of IP-10, IFN-? and IL-4 were measured by semi-quantitive RT-PCR with G3PDH as the internal control. Results The mRNA expression levels of IP-10, IFN-? and IL-4 as well as the rate of IFN-?/IL-4 were elevated in the PBMC of AS patients compared with the healthy controls (P

Objective To determine the potential deficiency due to lack of anaerobic culture and evaluate the effect to reduce adverse reaction associated to transfusion-translated bacterial infection.Methods The result of 9 758 units of apheresis platelet concentrates (PCs)detected with automated microbial detection system were reviewed and the medical records of the patients that received the contaminated PCs were followed.Results The confirmed positive rates by aerobic and anaerobic cultures were 0.06％ (6/9 758)and 0.16％ (16/9 758),respectively.In 10 of 16 yield cases,only the anaerobic culture was positive.The most of the bacterial detected by anaerobic culture only were Propionibacterium acnes.Their mean detection time from inoculation was 96.8±18.21 hours.Conclusion Addition of anaerobic culture would enhance the detection of bacterial contamination in PCs.However,since only slow-growing bacteria were detected,and because their clinical significance was debatable,blood service should select feasible and costeffective projects using only aerobic bottle for bacterial screening,like the majority of licensed blood centers in North America and Hong Kong,China.

Heparanase(HPA) is an endo-beta-D-glucuronidase that degrades heparan sulfate proteoglycans side chains.Recently data suggested a role of HPA in several proteinuric diseases and an increased glomerular basement membrane.The research advanced of the molecular properties,the role in proteinuric diseases and the specific inhibitor of the human HPA were described.

To introduce the achievements and experiences made by the Training Center of GP teachers.since its establishment,in training hospital-based GP teachers and community-based GP teachers,and to discuss problems occurred in the course of teacher training and resolutions for them.It suggests establishing encouragement mechanism for GP teaching and authentication system of GP teachers and enhancing the teaching capability of GP teachers.