Objective:To characterize the key dose response properties of the novel presage sheet dosimeters for radiotherapy dose verification, including absorption spectra, linearity, dose range and stability.Methods:The same batch of presage sheet dosimeters were irradiated by a radiotherapy linac. The absorption spectra within 400-700 nm were read out with a spectrophotometer, and the R-G-B3 absorption changes read out with a film flatbed scanner was compared before and after irradiation.Results:An absorption peak was clearly identified at 628 nm, where absorptions change in high linearity with delivered doses ( R2=0.9999). A flat valley region is identified around 490 nm, where dose induced absorption changes were negligible. The readout sensitivity of the R-channel of the flatbed scanner was higher than both in green and blue channels. In the dose range below 10 Gy, the R-channel absorptions are in significant linearity with doses ( R2=0.9999), with absorptions change in an obvious quadratic trend in the range beyond 10 Gy ( R2=0.9999). The dose range of presage sheets was more than 94.6 Gy. The absorptions were well preserved within 1 h post-irradation, and then are shown to increase gradually, where the increase speeds are dose-related. The post-irradiation integrity of dose falloff gradients are shown with negligible gradient blurring. Conclusions:The novel presage sheets shown to have reasonable dose response linearity, large dose range, desirable post-irradiation dose gradient integrity and negligible fractionation effect, which indicates its great potentials in integral dose verification of high-dose and multiple target radiotherapy deliveries.

Objective: To evaluate the therapeutic effect of dental autotransplantation with the application of digital design combined with 3D printing of donor tooth models and recipient alveolar fossa model preoperatively. Methods: Twelve cases that could not be retained due to tooth fracture or extensive absorption of alveolar bone were recruited in the study. Cone-beam computed tomography (CBCT) data were imported into Mimics software for digital design, and the best-matched third molar was selected as the donor tooth. Replicas of the donor teeth and the recipient socket were printed out with three-dimensional (3D) printing technologies as a simulation model for recipient tooth socket preparation. During tooth autotransplantation, preparation of the recipient tooth socket and the donor tooth were guided by the 3D-printed replicas sequentially. Then, the donor tooth was implanted into the recipient tooth pocket. Patients were followed up at 3, 6 and 12 months after the operation, with CBCT examination to evaluate the status of bone reconstruction and periodontal ligaments at each time point. Results: Twelve patients were transplanted with an autogenous third molar with the apical foramen completely closed. Among them, 7 patients had alveolar fossa infection before the operation, of which 1 had extensive resorption of the alveolar bone due to the infection. All 12 patients recovered well after the operation and were followed up for at least 12 months. In total, 11 caseswere successful in tooth autotransplantation with normal mastication, and 1 case had root resorption 14 months postoperation. Conclusion Digital design combined with 3D printing technology can assistin the selection of thebest-matched donor tooth and preparation of the recipient socket before tooth transplantation proceduresand reduce the extra-alveolar exposure time of the donor tooth and number of trial placementsintothe alveolar fossa. Thus, this combined strategy can effectively improve the outcome of dental autotransplantation.

Objective To analyze the research status,hotspots of biofilms and periodontitis from 2013 to 2023 by bibliometric methods.Methods The literatures related to biofilms and periodontitis were searched in the Web of Science(Wos)database,and visualization software such as VOSviewer and CiteSpace were used to analyze the countries or regions,authors,institutions,journals and key-words.Results A total of 1 821 papers were included,and the results showed that the number of published papers increased year by year.The top three countries with the highest number of publica-tions were the United States,Brazil and China;the top three institutions were Universidade de S?o Paulo,Harvard University and Forsyth Institute;the authors with the highest number of publications were Feres and Magda;among the top ten journals in terms of the number of publications,Journal of Periodontology had the highest number of publications(87 papers),followed by Plos One(69 pa-pers)and Clinical Oral Investigations(51 papers).Oxidative stress,markers,adjunctive therapy and drug delivery were the main research hotspots;the frontiers mainly focused on quorum sensing,photodynamic therapy,reactive oxygen species,copper nanoparticles,and optical coherence tomo-graphy.Conclusion Researches on biofilms and periodontitis are growing year by year,and future research directions can focus on oxidative stress,adjuvant therapies,quorum sensing and photody-namic therapy.

Objective To analyze the research status,hotspots of biofilms and periodontitis from 2013 to 2023 by bibliometric methods.Methods The literatures related to biofilms and periodontitis were searched in the Web of Science(Wos)database,and visualization software such as VOSviewer and CiteSpace were used to analyze the countries or regions,authors,institutions,journals and key-words.Results A total of 1 821 papers were included,and the results showed that the number of published papers increased year by year.The top three countries with the highest number of publica-tions were the United States,Brazil and China;the top three institutions were Universidade de S?o Paulo,Harvard University and Forsyth Institute;the authors with the highest number of publications were Feres and Magda;among the top ten journals in terms of the number of publications,Journal of Periodontology had the highest number of publications(87 papers),followed by Plos One(69 pa-pers)and Clinical Oral Investigations(51 papers).Oxidative stress,markers,adjunctive therapy and drug delivery were the main research hotspots;the frontiers mainly focused on quorum sensing,photodynamic therapy,reactive oxygen species,copper nanoparticles,and optical coherence tomo-graphy.Conclusion Researches on biofilms and periodontitis are growing year by year,and future research directions can focus on oxidative stress,adjuvant therapies,quorum sensing and photody-namic therapy.