This study was aimed to carry out the research by statistics to analyze the influence of specific clinical function of Dioscorea opposita Thunb. from the viewpoint of prescription in order to summarize its rules. Formula containing D. opposita Thunb. in the classic book of the Formula Dictionary of Traditional Chinese Medicine were recorded. The prescription type of D. opposita Thunb. was taken as the mainline. Excellwas used in the chart analy-sis. The results showed that for the function of reinforcing spleen-stomach, the effect of powder was increased, but the effect of pill was reduced. For the function of reinforcing lung and generating essence, the effect of decoction was significantly increased, but the effect of pill was significantly reduced. For the function of reinforcing kidney-yin, the effect of pill was significantly increased, but the effect of decoction was reduced. For the function of strengthening the kidney and restraining yin, the effect of powder was reduced. It was concluded that preparation had a strong in-fluence on the clinical function of D. opposita Thunb., which provided references for both clinical practice and health cultivation.

This study was aimed to optimize extraction process of formula granules of Clematis chinensis Osbeck and establish its qualitative discrimination method. With oleanolic acid, hederagenin and extract rate as indexes, extraction technology conditions of formula granules of C. chinensis Osbeck were investigated by L9 (34) orthogonal test method. TLC was used in the qualitative discrimination of formula granules of C. chinensis Osbeck. The results showed that optimum extraction technology was: adding 12 times of water; soaking for 0.5 h; cooking for 1 h; and extracting for 3 times. TLC had the diagnostic characteristic for the distinct spot which illustrated the specificity. It was concluded that the extraction process was reasonable, simple and feasible with high extraction efficiency of ef-fective contents, which can be the scientific basis for production and quality control of formula granules of C. chin-ensis Osbeck.

This article was aimed to study on clinical application of Shanyao (Dioscorea opposita Thunb.) in each historical period, which can offer some suggestions for clinical doctors to use Shanyao correctly. A total of 668 pieces of formulae containing Shanyao were recorded from the classic format Dictionary of Traditional Chinese Medicine Formula. And then, statistic analysis was made on the emerging frequency according to its effect. The results showed that different effects of Shanyao started to be widely applied from the Song dynasty. It was most widely applied in the Ming and Qing dynasty. Nowadays, the difference between using Shanyao has been gradually decreased. During the whole historical period, clinical functions of Shanyao are mainly reinforcing the spleen and the stomach, tonifying the kidney and strengthening yin. However, clinical functions of reinforcing the kidney and restraining yin, tonifying the lung and promoting fluid are used secondly. It was concluded that the research analyzed clinical application of Shanyao in each historical period, which can offer scientific basis for clinical practice and health maintenance with the using of Shanyao.

Endocrine Disruptors ; Environmental Exposure ; adverse effects ; Environmental Pollutants ; Humans ; Obesity ; epidemiology

Objective To explore the relationship between work engagement and job-person fit among nurses.Methods 465 nurses from five first-class general hospitals in Chengdu were assessed by the Utrecht Work Engagement scale(UWES)and the Areas of Worklife Survey(AWS).Results The mean value of work engagement among nurses was (3.21±1.09).Work engagement and job-person fit were positively related.Regression analysis suggested that job-person fits were significant predictors of nurses'work engagement.Exactly,value fit,workload fit,control fit and fairness fit were effective predictors of vigor.Value fit,fairness fit,reward fit and workload fit were effective predictors of dedication.Value fit,control fit,fairness fit and community fit were effective predictors of absorption.Conclusions The work engagement in nurses was at the moderate level.Job-person fits were powerful predictors of work engagement.Good fit among the six areas of work life can improve the work engagement among nurses.

Objective:To explore the application value of acoustic radiation force impulse (ARFI) imaging technology in the dif-ferential diagnosis of benign gallbladder wall thickening or gallbladder carcinoma. Methods:Siemens ultrasound ARFI, involving virtu-al touch tissue imaging (VTI) and virtual touch tissue quantification (VTQ), was used to examine the gallbladder wall in 327 cases. To compare transverse shear wave velocity (VTQ) of gallbladder waland VTI image characteristics. Results:Significant differences exist-ed in the gallbladder wall elastic parameters of normal and benign gallbladder wall thickening, as well as thick-walled gallbladder carci-noma. The ROC curve displayed that a velocity of 2.65 m/s can be used to diagnose gallbladder carcinoma, with sensitivity and specific-ity of 94.0%and 93.7%, respectively. Conclusion:The VTQ parameters were significantly different in benign and malignant gallblad-der wall thickening, thereby exhibiting clinical application value.

Exosomes are small lipid bilayer vesicle structure automatically secreted by diverse cell types in vivo which have been found to be released by various cell types and isolated from most body fluids and rich in protein,lipids and RNA.Exosomes are new medium of communication between cells,providing functional protein,the mRNA transcription and miRNA receptor cells,participating in the physiological and pathological processes of cells.It is considered as a biomarker of disease vectors In recent years,the clinical application value of exosomes attracted the attention of researchers in the monitoring of disease,autoimmune diseases,ischemic diseases,cancer and neurodegenerative disease treatment.This paper will focus on the current studies on exosomes in the diagnosis and treatment of the nervous system diseases.

[Objective] To observe the therapeutic effect of Shoutai Pill and Shixiao Powder for the treatment of threatened abortion with kidney deficiency and blood stasis. [Methods] Sixty inpatients and outpatients for quieting fetus in gynecology department of the First Affiliated Hospital of Guangzhou University of TCM were equally divided into 3 groups: group A was treated with Shoutai Pill and Shixiao Powder, group B with human chorionic gonadotropin (HCG) and group C with Shoutai Pill, Shixiao Powder and HCG. The effect on quieting fetus was evaluated by observing the disappearance time of symptoms such as vaginal bleeding, abdominal pain, lumbar ache, lower abdominal heaviness and distension. Changes of blood and urine HCG level and type B ultrasonic image were also examined to observe the effect and safety of Chinese medicine. [Results] The effective rate was 95% in group A, 70% in group B and 100% in group C, the difference being significant ( P0.05) . The result of follow-up showed that no terata was found in the three groups. [Conclusion] Chinese medicine with the actions of tonifying kidney and activating blood-stasis can prevent miscarriage and is safe without toxic and side effects.

Research on the application of ocular response analyzer has become one of the main issues in ophthalmology.The ocular response analyzer has been used in the measurement of ocular bioparameters of glaucoma,cataract,heretability and refractive surgery.As a newly invented non-contact tonometric device,the ocular response analyzer is helpful not only in the measurement of intraocular pressure (IOP),but also in the determination and calculation of two corneal biomechanical properties:corneal hysteresis (CH) and corneal resistance factor (CRF).It offers the possibility of measuring corneal biomechanical properties in vivo.This article primarily reviewed the application of the ocular response analyzer in terms of pre-operative examination and post-operative follow-up in excimer laser surgery.

Objective To make sure if there is single nucleotide polymorphisms(SNP) of c135 in RET proto-oncogene in Han ethnic group of Shaan Xi,China and investigate the relationship between Hirschsprung disease(HSCR) and SNP of RET proto-oncogene c135.Methods A total of 40 blood samples were collected from healthy donors through cluster sampling in Xi'an,Shaan Xi province.Sixteen tissues in aganglionic segments of HSCR were studied(they were all sporadic in Han ethnic group of Shaan Xi province of China and diagnosed by pathology).DNA was isolated from above samples and PCR amplified.The fragment of products were 300 case pairs(bp) which located in RET exon2 including c135.All of fragments were purified and directly sequenced and analyzed by related software and statistical methods.Results There really existed the single nucleotide polymorphisms of c135 A/G of RET in controls.A,G allele frequency was 0.475 and 0.525,respectively,and there was no distinctive dominant genotype.In patient group,A,G allele frequency was 0.844 and 0.156,respectively.c135A genotype was dominant in patients.There was significant statistical difference between patients and controls in RET c135A/G(PA.There is relationship between the disease and the c135 A genotype of RET.c135A genotype in the RET proto-oncogene are over-expressed in patients with Hirschsprung disease.