1.Content Determination of Resorcinol,Borneol, Glycerol and DMSO in Compound Fluocinonide Tincture
Yi ZHOU ; Jun LI ; Yingying WANG
China Pharmacist 2017;20(6):1144-1146
Objective: To establish a GC method for the determination of resorcinol, borneol, glycerol and DMSO in compound fluocinonide tincture.Methods: The analysis was performed on an HP-INNOWax column (30.0 m×0.32 mm,0.25 μm).The inlet temperature was 280℃ and the detector temperature was 290℃.The split ratio was 10∶1.With nitrogen as the carrier gas, the flow rate was 2.0 ml·min-1.The temperature was as follows: the initial temperature was maintained at 100℃ for 5 min and raised to 240℃ at a rate of 20℃·min-1, and maintaining for 8 min.The flow rate of hydrogen, air and tail blows was 35, 350 and 25 ml·min-1, respectively.Chennai was used as the internal standard.Results: The concentrations of DMSO, resorcinol, borneol and glycerol were within the range of 99.20-793.60 μg·ml-1 (r=0.999 9), 507.25-4 058.00 μg·ml-1 ,respectively(r=0.999 9), 102.20-817.60 μg·ml-1 (r=1.000 0) and 316.20-2529.60 μg·ml-1 (r=1.000 0) with a good linear relationship.The average recovery was 98.58%, 98.34%, 98.19% and 102.29%, and RSDs were 3.80%, 3.93%, 2.87% and 3.65% (n=9), respectively.Conclusion: The method is simple, accurate and reliable, and can be used for the quality control of DMSO, resorcinol, borneol and glycerol in compound fluocinonide tincture.
2.Analysis of DSA for inflammatory pseudotumor of the liver(analysis of 12 cases)
Weiming LEI ; Yi YANG ; Jun ZHOU
Journal of Interventional Radiology 1994;0(04):-
Objective To evaluate the diagnostic and differential diagnotic values of DSA features for inflammatory pseudotumor of the liver(IPL). Methods An retrospectively comparative analysis of 12 hepatic inflammatory pseudotumors confirmed surgicopathologically from May 2002 to March 2007 with DSA features and correlative imaging data were carried out. Results 10 cases consisted of only 1 lesion and other 2 cases with multiple lesions were opacified initially at the late arterial phase,with prominent peripheral ring-like staining at portovenous and parenchymal phases and less dense opacification in the central region; furthermore heterogenous staining occurred in 5 cases. Conclusions DSA is probable the most appropriate method for the diagnosis and differential diagnosis of hepatic inflammatory pesudotumor.
3.Advances in Study on Novel Oral Biological Agents in Treatment of Inflammatory Bowel Disease
Yi ZHOU ; Jun SHEN ; Zhihua RAN
Chinese Journal of Gastroenterology 2017;22(8):498-501
As a new therapy in past twenty years,biological agents have been approved for the treatment of inflammatory bowel disease (IBD). However,biological agents currently used for IBD treatment require intravenous or subcutaneous injections,and some require infusion under close observation. Therefore,it is of positive clinical significance to find a safe and effective oral biological agent. This article reviewed recent advances in study on novel oral biological agents in the treatment of IBD.
4. Amelioration of Tripterygium wilfordii Polycoride on TNBS/Ethanol-induced Ulcerative Colitis via Inhibiting Lipid Peroxidation and Its Downstream Inflammatory Meditors
Chinese Herbal Medicines 2017;9(4):344-352
Objective To study the protective effect of Tripterygium wilfordii polycoride (TWP) against TNBS/ethanol-induced ulcerative colitis (UC) rat model. Methods TNBS and ethanol enema were adopted to build TNBS/ethanol-induced UC rat model. Ninety male Wistar rats were divided into six groups: normal, model, low-, medium-, high-dose TWP and azathioprine (AZA) groups, each for 15 rats. All rats were administered by corresponding medicine for 14 d. After 14 d, corresponding colon tissues underwent general and microscopic evaluation. Blood samples were taken from heart and serum was separated by centrifugation. MDA, SOD, GSH, IL-1β and TNF-α levels in serum were tested by ELISA. Colonic samples underwent RT-PCR and Western blotting analysis. Results DAI, general and microscopic evaluation all showed that TWP could promote colonic mucosa healing and such effect was equal to AZA. ELISA results about lipid peroxidation indicated that TWP could decrease MDA level and increase SOD and GSH levels in a dose-dependent manner. TWP with high dose could strongly decrease the MDA level and increase the SOD and GSH levels (P < 0.01). ELISA results about inflammatory cytokines indicated that TWP could inhibit the expression of IL-1β and TNF-α in a dose-dependent manner. Western blotting analysis and RT-PCR all indicated that no matter in mRNA level or protein level, TWP could inhibit the expression of NF-κB, TNF-α IL-1β and IFN-γ in a dose-dependent manner. The inhibitory effect of AZA towards NF-κB was slightly weaker than TWP with high dose (P > 0.05), whereas slightly stronger towards terminal inflammatory cytokines (P > 0.05). Conclusion TWP could significantly lower the infiltration of inflammatory cells under microscope, eventually led to mucosa healing, the mechanism of which was to inhibit lipid peroxidation, then further inhibit NF-κB activation, eventually lower the expression of inflammatory meditors locally and systemically.
5.A New Quantitative Determination Method for the Biological Activity of Recombinant Human Ciliary Neurotrophic Factor
Hu ZHOU ; Yi WANG ; Jun-Ming ZHU ;
China Biotechnology 2006;0(02):-
Objective: To develop a new quantitative determination method for the biological activity of recombinant human ciliary neurotrophic factor. Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally. Result: rhCNTF could promote original era dorsal root neuron cells of chick embryo surviving,the livability of neuron cells was positively related to the amount of rhCNTF added to the culture. Conclusion: A quantitative determination method for the biological activity of rhCNTF was developed by testing the activity of acid phosphatase in neuron cells. Compared with the typical ways,this method was quantificational easily,repeatable better and with much fewer disturbance factors.
6.Experimental study of biocompatible and osteoinductive behavior of hydroxyapatite/polyethylene
Yi-Di WANG ; Dong-Mei LI ; Yi QIN ; Jun ZHOU ;
Ophthalmology in China 2006;0(06):-
Objective To explore the histocompatibility and plasticitity of hydroxyapatite/high-density polyethylene composite (HMPE). Design Experimental study. Participants Forty-five rabbits. Methods Rabbits were randomly devided into HA/PE, HA and PE surgical groups, every group included 15 rabbits. Osteoeetomy of orbital rim and implantation (HA/PE, HA and PE materials were used) were performed on 3 groups of the rabbits. At 1st week, 4th week, 8th week, 12th week and 24th week of postoperation, we took out the samples from the rabbits separately in each group and did histopathologic and electron microscope examinations. Main Outcome Measures Physical signs of the implant combination with the bone. The bone cell appeared in the implants. Results After 12 weeks, the calcium salts deposit like bone plate with some osteoblast inside the HA/PE implants was observed. After 8 weeks, the calci- um salts deposit inside the HA plants was observed. In the PE implants, nothing could be seen but some fibrous connective tissue. Conclusion HMPE is an ideal repair material especially for orbital bone.
7.Reconstruction and measurement of a digital dental model using grating projection and reverse engineering.
Zhenzhen WANG ; Yi LU ; Jun SONG ; Jun CHEN ; Qin ZHOU
West China Journal of Stomatology 2015;33(1):71-74
OBJECTIVEThis work lays the foundation for establishing a digital model database with normal occlusion. A digital dental cast is acquired through grating projection, and model features are measured through reverse engineering.
METHODSThe grating projection system controlled by a computer was projected onto the surface of a normal dental model. Three-dimensional contour data were obtained through multi-angle shooting. A three-dimensional model was constructed, and the model features were analyzed by using reverse engineering. The digital model was compared with the plaster model to determine the accuracy of the measurement system.
RESULTSThe structure of three-dimensional reconstruction model was clear. The digital models of two measurements exhibited no significant difference (P > 0.05). When digital and plaster models were measured, we found that the crown length and arch width were not statistically different (P > 0.05), whereas the difference between the crown width and arch length was statistically significant (P < 0.05).
CONCLUSIONThe reconstruction of a digital model by using the grating projection technique and reverse engineering can be used for dental model measurement in clinic al and scientific research and can provide a scientific method for establishing a digital model database with normal occlusion.
Dental Models ; Dental Occlusion ; Imaging, Three-Dimensional
8.Modified Preparation and Stability of Compound Menthol Spray
Yilu CHEN ; Yi ZHUO ; Jun ZHOU ; Yue HUANG ; Suilin LI
China Pharmacy 2001;0(12):-
OBJECTIVE:To improve the preparation of compound menthol spray and study its stability.METHODS:The liquor compound menthol spray was changed to emulsified ones by the solubilizing action of tween80.The stability of emulsified compound menthol spray(ECMS)was observed in airtight container at room temperature(25?2)℃.RESULTS:ECMS was stable within2wks in airtight container at room temperature.CONCLUSION:ECMS is stable in property,convenient in stor?age,and accords with pharmaceutical quality standards.
9.Association of ER? expression with the clinical pathologic indexes and prognostic biomarkers of breast cancer
Yan ZHOU ; Yi ZHANG ; Yan LIANG ; Jun JIANG
Journal of Third Military Medical University 2003;0(11):-
Objective To study the association of ER? expression with the expression of hormonal receptor and prognostic biomarkers.Methods The expressions of ER?,ER?,PR,Ki-67,HER-2/neu and p53 were detected in tissue samples from 109 breast cancer patients by immunohistochemistry on tissue microarray slides.The histologic grade,tumor size and lymph node metastasis status of 109 cases of breast cancer were recorded and served as comparison indexes.Results ER? was expressed in benign epithelium and stroma cells,and breast cancer cells in 59% breast cancer tissues of different histologic types.ER? was coexpressed with ER? in 45% breast cancer tissues.ER? expression was found a statistically significant association with HER-2/neu(P
10.Effects of Dexamethasone on Changes of CD4~+,CD8~+ Lymphocyte in Children with Viral Encephalitis
yu-miao, ZHANG ; rong, YANG ; fang-jun, LUO ; yi, ZHOU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To observe the effects of dexamethasone (DEX) therapy on the clinical outcome and changes of lymphocyte subsets in children with mild and severe encephalitis.Methods Eighty-two children with mild and severe viral encephalitis were randomly divided into two groups: with DEX treatment (n=46) or without(n=36).The clinical efficacy was evaluated 3 weeks later, and the clinical manifestation were also observed. The changes of CD4~+,CD8~+ T lymphocyte percentage were determined by flow cytometry on admission and at 1 week,4 weeks after treatment.Another group of 20 cases was enrolled as control group.Results Compared with the control group,both the DEX treatment group and non-DEX treatment group showed a reduced CD4~+ lymphocyte count, an increased CD8~+ lymphocyte count(P