Objective To investigate the imaging characteristics of primary abdominal cocoon in order to improve diagnosis level.Methods The imaging data of 5 cases of primary abdominal cocoon proved by surgery and pathology were retrospectively analyzed.Results Abdominal plain X-ray suggested intestinal obstruction in 3 out of 5 cases.The gastrointestinal barium meal showed cauliflower signal in all 5 cases;CT suggested a conglomeration of multiple small bowel loops in the 5 cases and the intestinal loops seemed to be encapsulated in a thickened capsule.Conclusion Abdominal cocoon should be taken into consideration when gastrointestinal barium meal shows cauli flower signal and CT displays conglomeration.

Objective To explore the diagnostic value of multislice spiral CT enterography(MSCTE) in patients with primary tumors of the small intestine.Methods Clinical data of 40 patients with primary tumors of the small intestine from Jan.2011 to Dec.2013 were analyzed.All patients were underwent MSCTE examination.Analyzed the coincidence rate between diagnostic results and pathological diagnosis.Results Pathological diagnosis of all patients showed that 15 cases were with small intestinal stromal tumors,10 cases with primary intestinal adenocarcinoma,9 cases with intestinal lymphoma and 6 cases with leiomyoma.The diagnoses of MSCTE were as follows:Twelve cases were with gastro intestinalstromaltumors,10 cases with primary intestinal adenocarcinoma,9 cases with intestinal lymphoma and 9 cases with leiomyoma.Consistency test(Kappa test) results showed that there was a good consistency between two dignosis methods(Kappa values =0.71).The coincidence rate of MSCTE in the diagnosis of primary tumors of the small intestine was 92.5％.Conclusion MSCTE is a convenient and safe method with high degree of accuracy,which provides a broad prospect for the diagnosis of primary tumors of the small intestine.

OBJECTIVE:To establish an HPLC method for the determination of diphenyl ethylene glycoside in Yishi oral solution. METHODS:The sample was separated on a DiamonsilTM C18 column(200 mm?4.6 mm,5 ?m) with the mobile phase consisted of acetonitrile-0.1% phosphoric acid(18∶82) at a flow rate of 1.0 mL?min-1.The detection wavelength was 320 nm and the column temperature was 40 ℃.RESULTS:The linear concentration range of diphenyl ethylene glycoside was 65.25～625.50 ng(r=0.999 9) and the average recovery rate was 100.07%(RSD=0.25%,n=6). CONCLUSION:The method was proved to be simple,re-producible and simple in operation,and it can be used for the quality contro1 of Yishi oral solution.

OBJECTIVE To understand the status of hand hygiene and hand-washing compliance in grass-roots hospitals and investigate the relation between hand hygiene and hospital infections to institute the administration strategy of hand hygiene.METHODS Hand contamination of medical staff in grass-roots hospitals was investigated.The species carrying were detected and the reason was analyzed.RESULTS The carrying rate of pathogenic bacteria before hand-washing was 100.0% while that after hand-washing was 36.5%.The pathogenic bacteria carrying included: Staphylococcus aureus,Escherichia coli,Klebsiella pneumoniae,Proteus,Pseudomonas aeruginosa,P.alcaligenes,coagulase negative Staphylococcus,Enterococcus,etc.CONCLUSIONS The hand contamination is the important risk factor of cross infection.We should streng then hospital infections idea among medical staff,think highly of supervision of hand hygiene and enhance the recognition and compliance of hand-washing to eliminate effectively the cross infection transmitted through hands.

ObjectiveTo investigate the effect of bone marrow-derived mesenchymal stem cells (MSCs) on collagen-induced arthritis(CIA) and the possible mechanisms. MethodsMSCs from C57BL/6 mice bone marrow were isolated and expanded. Three DBA-I mice were as normal control group (Sodium Chloride injected on day 0 and 21) after primary immunization. After the bovine collagen Ⅱ induced-CIA models were established, fifteen male mice were randomly divided into 3 groups according to treatment regi-mens: CIA control group(0.2 ml Sodium Chloride injected through caudal vein on day 0 and 21), prevention group(1×10+6 cells MSC on day 0) and treatment group. (1×10+6 cells MSC on day 21 after primary immun-ization). The observed parameters included arthritis index (Al), articular pathology changes, levels of serum TNF-α, IL-1β detected hy ELISA. In addition, the percentage of CD4+CD25+Foxp3+Treg cells(Tregs) in CD4+ T cells of spleen or lymphoid node from mice were also analyzed by flow cytometry(FCM). Results①The Al and articular pathological score of the prevention group and treatment group were lower than the CIA control group(P<0.05). ②The levels of serum TNF-α and IL-1β of normal control, prevention group and treatment group were significantly decreased compared with CIA control group(P<0.05), and positively correlated with Al or articular pathological score (P<0.05). ③The percentage of Tregs from spleen and lymph nodes was significantly increased in MSCs treated mice(P<0.05). Conclusion MSCs transplantation is effective for CIA and suppression of TNF-α, IL-1β and up-regulation of Tregs by MSCs may be the possible mechanism in CIA.

Acute kidney injury(AKI) is defined as functional and structural disorder or signs of renal damage including any defect from blood and urine test,or tissue imaging that is less than 3 months.The current treatment for AKI includes therapeutic agents and renal replacement therapy (RRT).The goal of RRT is to remove endogenous and exogenous toxins,inflammatory mediator,pathogenic factor,and to maintain fluid,electrolyte,and acid-base balance until renal function returns.The principal methods of RRT include intermittent hemodialysis,continuous renal replacement therapy,peritoneal dialysis,plasmaphresis exchange and hemoperfusion.In this article,we discussed the timing,choice of therapeutic mode,indication and common technical issues of RRT in pediatric AKI.

Objective To investigate the changes of plasma vascular endothelial growth factor( VEGF) of patients of advanced primary liver cancer before and after interventional treatment and its clinical Significance. Methods Forty-six patients with advanced primary liver cancer were performed the hepatic arterial chemoembolization and served as observation group. Sixty healthy people were served as control group and measured their level of serum VEGF. The level of serum VEGF of patients in observation group before treatment and intervention 1 month were measured. Results Before interventional treatment,the serum VEGF level of observation group were(510. 0 ± 310. 0)ng/L,significantly higher than that of control group((27. 0 ± 23. 0) ng/L,P﹤0. 001). After interventional treatment,he serum VEGF level decreased to(316. 4 ± 240. 7)ng/L and the difference was statistically significant( P=0. 036 ). After one month interventional treatment,4 cases were complete remission(8. 70%),34 cases were partial remission(73. 91%),6 cases were in stable(13. 04%),and 2 cases were progress( 4. 35%),and the total effective rate were 82. 61%( 38/46 ). The 38 cases who were complete remission and partial remission were been served as effective group,the other 8 cases were served as invalid group. The serum VEGF level in effective group were( 260. 6 ± 70. 5 )ng/L,lower than that in invalid group(( 346. 7 ± 61. 3 )ng/L,P = 0. 046 ). Conclusion Hepatic arterial chemoembolization treatment on primary liver cancer curative effect is a better approach. VEGF involves in the development of primary liver cancer,and the high serum VEGF level with poor prognosis can be the reference of efficacy.

Interleukin-10 (IL-10) is an immuno-regulatory cytokine,which is well known for its immunosuppressive function.IL-1O can act on a variety of immune cell subsets and play an immunosuppressive effect through a variety of ways.It plays an important role in the tumor immune escape.Recent studies show that IL-10 also has an immune-stimulating function,which is presumably a pluripotent cytokine.This paper will combine the latest literature to summary the positive and negative regulatory roles of IL-10,mediation ways in the tumor and the relationships with prognosis.

Objective To investigate the clinical efficacy of gemcitabine and cisplatin chemotherapy combined with CT guided microwave ablation used in treatment of advanced squamous cell lung cancer. Methods 76 patients with lung squamous carcinoma in our hospital from April 2013 to July 2015 were selected,and randomly divided into treatment group and control group,38 cases in each groups. The control group were treated with gemcitabine and cisplatin chemotherapy. The patients in the treatment group in the control group based on the combined CT guided ablation under microwave, after the end of treatment to evaluate the efficacy of the two groups of patients, observe two groups of patients with adverse reactions, and were followed up for 1-2 years. Results The total effective rate of treatment group was significantly higher than the control group (P<0.05); pneumothorax treatment group after microwave ablation in patients with 2 cases, 3 cases of hemoptysis, 2 cases of fever, recovered after symptomatic treatment. The adverse reactions of the two groups due to chemotherapy caused by comparison of the rate of no significant difference; the treatment group of patients with PFS was significantly longer than the control group, the 1 year survival rate and 2 year survival rate was significantly higher than the control group(P<0.05). Conclusion Gemcitabine combined with cisplatin chemotherapy regimen combined with CT guided microwave ablation is effective in the treatment of advanced squamous cell lung cancer, and is worthy of clinical promotion.

Objective To study the mechanism of the effects of db cAMP on the inhibition of cell proliferation and phenotypic changes in transformed cells. Methods The progression of cell cycle was analyzed by the flow cytometry.The transforming phenotype of C 3H 10 T 1/2 cells was observed by the soft agar colony formation.Northern blotting and radioimmunoassay were used to analyze the level of CaM expression.The activity of PKⅡ was determined through ? 32 p ATP incorporation assay. Results The proliferation of transformed C 3H 10 T 1/2 cells was inhibited markedly by 1mmol/L db cAMP.The db cAMP treated transformed cells were accumulated at G 1 phase.After treatment with db cAMP,the soft agar colony formation efficient in transformed cells was decreased evidently,simultaneously,cells altered in morphology,from rounded to flattened shape.In contrast to normal cells,CaM level and PKⅡ activity increased in transformed cells.However,both CaM level and PKⅡ activity in transformed cells treated by db cAMP decreased obviously.Conclusion\ The results indicate that not only alteration of CaM expression,but also the changes of the PKⅡ activity are involved in account for the cell transformation and induced differentiation of transformed cells.It is possible that the role for CaM in the cell transformation and induced differentiation may be mediatied by the effect of PKⅡ. \;[