The bilingual teaching is a new mode to enhance the synthetic ability of the medical students' foreign languages.The article proposes several manners of guarantying the teaching effect such as strengthening the teacher's quality,applying multimedia to teaching,using unified teaching material and increasing students' interest in learning.

Objective To explore the relationship between mindfulness and rumination of nursing students in clinical practice. Methods Three hundred and sixty two nursing students participated in the survey using Chinese version of mindfulness scale and rumination scale.Results The scores on mindfulness and rumination among the 362 nursing students were 3?18 ± 0?37 and 1?62 ± 0?19,respectively.The mindfulness among them was negatively correlated with their rumination and so it were with the three dimension of mindfulness:awareness in action,non-reactiveness of inner experience and non-judgment on inner experience with the 3 dimensions of rumination(P<0?01 or P<0?05).The 3 dimensions of mindfulness predicted the variance of rumination by 31?1%.Conclusions The mindfulness of nursing students is at moderate level,and their rumination at low level.Therefore,the nursing administrations or educators should further cultivate mindfulness of nursing students to reduce the extent of rumination?

This paper summarized the nursing care of 6 patients with severe aplastic anemia complicated with DIC. The risk of sepsis and severe pneumonia can be reduced through actively nursing and preventing of oral,res-piratory,skin and perianal infections. The chances of bleeding from the skin,nose,and mouth can be reduced by less skin puncture,local medication,cold compresses and compression. Also by monitoring the coagulation indexes, estimating the phase of DIC and observing the bleeding manifestations. Through controlling heparin dripping rate, monitoring coagulation index,reporting to doctors at any time and knowing how to deal with heparin overdose,can increase the safety of heparin administration and improve the clinical application rate of early DIC heparin therapy. Psychological guidance can reduce the risk of cerebral hemorrhage caused by excessive exertion and accidental fall. After treatment and careful nursing,6 patients improved,5 cases were discharged,and 1 case died.

Objectives:To study the expression of PTEN in endometrial carcinoma under different pathological factors and to discuss its relationshipwith the occurrence of endometrial carcinoma and the clinical significance.Methods:The expressions of PTEN in 40 cases of endometrial carcinoma,10 cases of atypical hyperplasia and 10 cases of normal endometria were examined by immunohistochemical method.Result:The positive staining rate of PTEN in endometrial carcinoma was 55.00%,and that in normal endometria was 100.00%.There was no obvious correlation between the expression of PTEN in endometrial carcinoma and different pathological factors such as clinical staging,depth of myometrial invasion and lymph node metastasis.Conclusion:The expression lack of PTEN exists in endometrial carcinoma,and PTEN has an important role in the occurrence of endometrial carcinoma.

The software design of a biological enzyme calibration system based on virtual instrument technology is introduced. The serial communication with LabVIEW and data tele -distribution with DataSocket technology are also described.

133 teeth in 34 children aged 4 to 8 year-old were recruited for the treatment of bilateral maxillary primary canines or primary molars with deep caries,chronic pulpitis or chronic periapical periodontitis under local anesthesia.Buccal infiltration(BI)with conventional syringe(CS)was used for one quadrant,anterior middle superior alveolar anesthesia(AMSA)with computer-controlled local anesthesia de-livery system(C-CLADS)on the contralateral side.The injection duration(s)of CS and C-CLADS was 63.6 ±22.6 and 136.6 ±12.4 re-spectively(P =0.000).The pain perception of C-CLADS injection was significantly lower than that of CS(P <0.05),that during operation was not statistically different(P >0.05).55.9% children preferred C-CLADS anesthesia.

Objective To investigate the blood glucose control level,the characteristics of complications and treatment programme in senile patients with type 2 diabetes.Methods From Aug 2010 to Jul 2011,gender,age,height,weight,disease course and underlying disease were recorded in 119 patients with type 2 diabetes aged 80 years and over.The levels of glycosylated hemoglobin (HbA1c),fasting C-peptide (FC-P) and 2 hours postprandial C-peptide (2h C-P),urinary albumin excretion rate (UAER) were tested.Ultrasonic Doppler examination of lower-limb arteries as well as non-mydriatic colour eye-ground photography were also examined,peripheral nerve lesions were evaluatcd according to the criteria of diabetic neuropathy system (DNS).The patients were divided into two groups:HbA1c＜7.0％ group (65 cases) and HbA1c ≥ 7.0 ％ group(54 cases).Results The disease course[(12.1±8.5)years vs.(16.6±7.5)yeas,t=3.01,P=0.003],UAER[(20.2±11.9)μg/min vs.(47.0±21.4)μg/min,t=2.48,P=0.015] and incidence of diabetic nephropathy [23.1％(15/65)vs.50.0％(27/54),x2=9.36,P=0.002] were all less in HbA1c＜7.0％ group than in HbA1c ≥ 7.0％ group,while the levels of FC-P and 2hC P were higher in HbA1c＜7.0％than in HbA1c ≥ 7.0％ group[(2.1±1.2)μg/L vs.(1.5±1.0)μg/L,t=1.87,P=0.042; (6.5± 3.3)μg/L vs.(4.3 ± 2.9) μg /L,t =2.10,P =0.037].The proportion of patients who took oral hypoglycemic agents was increased while those who received insulin therapy decreased in HbA1c＜7.0％ group (all P=0.000).The α-glucosidase inhibitors were the most commonly used in each group,followed by metformin and insulin secretagogue,and the thiazolidinediones were rarely used,while there was no significant difference in the proportion of medicine between the groups.The overall incidence of symptomatic hypoglycemia was 23.5％(28/119).It was higher in HbA1c ≥ 7％ group than in HbA1c＜7.0％ group[33.3％(18/54)vs.15.4％(10/65),x2 =5.20,P=0.022].However,71.4％(5/7)of the patients with hypoglycemic coma came from HbA1c＜7.0％ group.Conclusions There are obvious individual differences in blood glucose control and complications among senile type 2 diabetes patients,thus the characteristics of the elderly should be considered in the choice of drugs while hypoglycemic events should be paid more attention.

Objective To explore the psychological intervention on the anxiety of patients under gastroscope examination. Methods According to the self-rating Anxiety Scale (SAS), 100 patients with anxiety disorder were randomly divided into observation group 50 cases, a control group of 50 cases, check the two groups of patients before, during and after adopt routine health education, observation group given psychological intervention at the same time, compared the two groups of patients with anxiety, check the time and satisfaction. Results After the psychological intervention, the scores of self-rating anxiety scale of observation group were significantly lower than those of control group, there were signiifcant differences (P<0.05), check time was shortened obviously, the difference was statistically signiifcant (P<0.05), satisfaction was also signiifcantly better than the control group the difference was statistically significant (P <0.05). Conclusion Psychological intervention can reduce the anxiety level of patients with gastroscope, shorten the examination time of gastroscope, increase the ability of patients to understand the disease, improve patient satisfaction.

Objective To investigate the relationship between PrfA-dependent promoters and PrfA regulation. Methods LacZ reporter gene fusions used to investigate the inhibitory elements for PrfA-dependent transcription were carried on two promoters of Listeria monocytogenes: a PrfA-dependent promoter of the phospholipase gene pica (PplcA) and a putative promoter of the aroA gene (ParoA2) which contains a similar PrfA-binding site (PrfA-box) and a similar-10 box as PplcA but does not function as PrfA-dependent promoter. A series of hybrid plcA-aroA promoters by exchanging corresponding sequence elements of these two "promoters" were constructed and incorporated into upstream of a promoterless lacZ gene. The variant promoter-lacZ transcriptional fusions were then electroporated into L. monocytogenes wild-type strain P14, prfA mutant P14a and prfA deletion mutant A42, respectively. The expression level of PrfA is the highest in the P14a and the lowest in A42. The corresponding transcription activities of hybrid promoters were measured by the β-galactosidase assay. Results The two critical elements of PrfA-dependent promoters, the PrfA-box and the-10 box, can be functionally exchanged as long as the distance in between is maintained 22 or 23 bp. However, the interspace sequence and the sequence downstream of the -10 box of ParoA2 were strongly inhibitory for PrfA-dependent transcription. Conclusion Downstream sequence together -10 box of ParoA2 might fold into a hairpin structure when present in a single stranded DNA and possibly block the formation of the transcriptional initiation open complex, hence, inhibit the PrfA-dependent transcription from ParoA2.

Resveratrolisonekindofpolyphenoliccompoundswidelyexistingingrapesandother plants.Recent studies have documented that resveratrol may play anti-tumor role by mechanisms of anti-oxi-dant,cyclooxygenase inhibition,anti-proliferation,differentiation and apoptosis in gynecological tumors,such as cervical cancer,ovarian cancer and endometrial carcinoma.