Osteocalcin is a bone matrix noncollagen protein which is one of the most abundant secretory products of osteoblasts. Resent studies have demonstrated that osteocalcin is not only a specific biochemical marker of bone turnover, but also involved in the regulation of energy metabolism. Further studies in the pathophysiological functions of osteocalcin might contribute to the treatment of obesity, diabetes, and metabolic syndrome.

Acupuncture Therapy ; Adult ; Bursitis ; immunology ; therapy ; Chronic Disease ; therapy ; Female ; Hip Joint ; immunology ; Humans ; Male ; Middle Aged

Objective:To study factors influencing drug abuse in f emale.Method:230 female drug abusers admitted into Institute of Dr ug Withdrawal were investigated by Drug Abuse Inventory made by Chinese Drug Depend ence Institute.Data was analyzed by Logistic analysis.Results:Risk f actors related to drug abuse included:poor education,unemployment,divorce or sep aration,stimulus seeking,destructive social relationship,meaningless life.Conclusion:Drug abuse in female is a problem occurring on poor psychosoci al background.

Objective To discuss the value and the effect of radiofrequency treatment of tennis elbow under the arthroscopy. Methods From March 2003 to Jan 2004,eight cases of tennis elbow were treated by TOPAZ with local anesthesia under arthroscopy. Results The effects and functions were satisfactory after radiofrequency treatment. The symptoms of pain were completely disappeared in 7 and obviously relieve in 1. No blood vessel and nerve injury or infection occurred. Conclusion The methods of radiofrequency therapy for tennis elbow under arthroscopy has the advantage of minimum invasion, less pain and earlier rehabilitation.

The genes for TEM and aminoglycoside-modifying enzymes (AMEs) in Acinetobacter baumannii were studied to provide molecular biological support for monitoring their drug resistance.MethodsTwenty strains of Acinetobacter baumannii were clinically isolated from the period of August, 2002 to Feburary, 2004. The drug resistance of these strains to thirteen kinds of antimicrobial agents was tested by microdilute test. The TEM and AMEs genotypes of these strains were analyzed by polymerase chain reaction and verificated by DNA sequencing .Results All twenty strains were sensitive to Imipenam and Meropenem. The resistant rates of the twenty strains to Piperacillin/ Tazobactam, cefepime and ceftazidime were 45.0%, 65.0% and 70.0%, respectively. The resistant rate to aminoglycosides antibiotics was 75.0%. The resistant rates to other antimicrobial agents were greater than 65.0%. The positive rate of genotype TEM, aac(3)-Ⅰ, aac(3)-Ⅱ aac(6”)-1 and ant(3”)-Ⅰ were 45.0%, 55.0%, 15.0%, 35.0%, and 60.0%, respectively. The genotypes of SHV, AmpC DHA and AmpC MIR were all tested negative.Conlusions The multiple-drug-resistance of Acinetobacter baumannii isolated from Tongde Hospital was a serious issue.There were high percentages of TEM and AMEs genotypes. Because of the imperfection frame of the primer,some familiar genes just like OXA,VER and PER were not experimented. We shall further discuss the drug resistance of the third/fourth generations cephalosporin.

In recent years,nuclear medical imaging has become a routine examining technology.In general,angiographic medicine has to be injected into human body.Considering the short-coming of the manual injection,it seriously needs a injecting pump with the functions such as auto-control,push-pull injecting,synchronous injection of two injectors.

[Objective]To determine the efficacy and characteristics of percutaneous vertebroplasty in treating patients with vertebral osteoporosis fractures combined with intraosseous cyst.[Method]Thirteen cases of vertebral osteoporosis fractures combined with intraosseous cyst were performed with percutaneous vertebroplasty.Bone cement containing appropriate proportion allograft bone powder were injected to vertebral body according to the sererity of osteoporosis and the size of intraosseous cyst.[Result]According to standard of World Health Organization about pain,complete pain relief was in 10,partial in 2,and slight in 1 patient.One case developed bone cement leakage into the paravertebral soft tissues during operation,but there were no clinical signs and symptoms.The next vertebral body fracture was found at sixteen days after percutaneous vertebroplasty in 1 case,and percutaneous vertebroplasty was repeated to relieve his pain.This patient was followed-up for 1 year,and no refracture was observed.[Conclusion]Vertebral osteoporosis fractures combined with intraosseous cyst is a special disease in elderly population.Percutaneous vertebroplasty is effective and it shouled be the first option for treatment of patients with vertebral osteoporosis fractures combined with intraosseous cyst.The complications could be reduced by local treatment combined with anti-osteoporosis drugs and correct rehabilitation.

[Objective]To explore the clinical effects of selecting differents type of tendon transplants to reconstruct anterior cruciate ligament(ACL) using press fit technique.[Method]A total of 101 cases of ACL injury were included in this study,62 cases were athletic injuries,22 cases were traffic injuries,and 17 cases were daily-life injuries.According to patient's condition using different types of tendon transplant in which hamstring-tendon-knot 42 cases,bone-bolt-hamstring tendon knot 32 cases,patella bone-quadriceps tendon 27 cases,the tendon-knot was completely fit-into the bottle-necked femoral tunnel,and the tibial tunnel were pulled ont and seperated into two bundles, then tie a knot to fix the tendons tightly onto the bone bridge.[Result]In this group 98 patients were followed-up,According to the knee joint effective evaluate standard,88 were excellent,6 good and 4 fine.Excellent and good rate was 85.7%.[Conclusion]According to patient's condition,different type of tendon transplant to reconstruct ACL by press fit technique should be selected carefully.It is an method of easy to manipulate,reliable fixation,minimized trauma,biological fixation,economical and free expensive implant technique.

Objective To investigate the outcomes of arthroscopically lateral retinaculum patellae release and medial patellofemoral ligament reconstruction using the semitendinosus tendon autograft for the treatment of habitual or recurrent dislocation of the patella. Methods 18 cases of recurrent dislocation of the patella were included in this group, among which there were 6 males and 12 females. The average age was 20 years old(range from 17 to 24 years). Arthroscopic investigation found tensed lateral retinaculum patellae and damaged cartilage at patellar medial margin and femoral lateral condyle. There were 6 cases of Ⅱdegree cartilage injuries, 5 cases of Ⅲ degrees injuries, and 7 cases of Ⅳ degree injuries caused by patellofemoral joint impact. For cases of Ⅳ degree injuries, the damaged cartilage was shaved and microfractured. For cases of Ⅱ or Ⅲ degree injuries, radiofrequency system was used for debridement. After the lateral retinaculum patellae was extensively released, semitendinosus tendon autograft was used to reconstruct the medial patellofemoral ligament. The knee joint was flexed and extended. Patella's dynamic motion, the tension of the tendon and relationship between the femoral trochlea and patellar bone were arthroscopically observed. Interface screw was used to fix the tendon ends within the bone tunnel on condylus medialis femoris. Results All patients were followed up for 9 to 19 months averaging 13 months. No patellar dislocation occurred postoperatively. Apprehensive test, patella tilt test were negative; and Apley test were negative in 16 patients and positive in 2 patients. One case of inferior pole of patella fractured. Axial view of X-ray and MR images of patella showed 15 cases regained normal anatomical relation of patellofemoral joint. 3 cases had mild semi-dislocation. Lysholm's score averaging (78.0?4.5) points preoperatively and (94.0?3.5) points postoperatively. Conclusion Lateral retinaculum patellae solution and medial part reconstruction under arthroscope can effectively cure recurrent subluxation of the patella and improve knee joint function.

Objective To investigate the pattern and the role of cytoskeletal genes expression during nerve regeneration. Methods We examined the changes of expression of ? tubulin(? Tub)and three neurofilament(NF)subunits mRNA in rat corticospinal neurons of sensorimotor cortex on the 1st,3rd,5th,7th,10th,14th,21st,28th and 56th days after spinal cord semitransection injury by using in situ hybridization. Results Levels of ? Tub,NF L,NF M and NF H mRNA were dramatically downregulated within large sized pyramidal neurons in layer V of the sensorimotor cortex of side lateral after spinal cord injury.The level of ? Tub mRNA was decreased as early as 1 day postinjury,whereas the levels of NF subunits mRNA were not decreased until later time(on the 3rd days postinjury),and both of them did not return to control levels on the 56th days following the lesion. Conclusion ? Tub gene expression if inhibited in CNS neurons postinjury. The axotomy signeal and the continuance downregulation of NF mRNA expressioon following spinal cord injury may contribute to the ineffective regeneration response of CNS neurons.