Osteocalcin is a bone matrix noncollagen protein which is one of the most abundant secretory products of osteoblasts. Resent studies have demonstrated that osteocalcin is not only a specific biochemical marker of bone turnover, but also involved in the regulation of energy metabolism. Further studies in the pathophysiological functions of osteocalcin might contribute to the treatment of obesity, diabetes, and metabolic syndrome.

Acupuncture Therapy ; Adult ; Bursitis ; immunology ; therapy ; Chronic Disease ; therapy ; Female ; Hip Joint ; immunology ; Humans ; Male ; Middle Aged

Objective:To study factors influencing drug abuse in f emale.Method:230 female drug abusers admitted into Institute of Dr ug Withdrawal were investigated by Drug Abuse Inventory made by Chinese Drug Depend ence Institute.Data was analyzed by Logistic analysis.Results:Risk f actors related to drug abuse included:poor education,unemployment,divorce or sep aration,stimulus seeking,destructive social relationship,meaningless life.Conclusion:Drug abuse in female is a problem occurring on poor psychosoci al background.

Objective To observe the effect of exogenous basic fibroblast growth factor (bFGF) on apoptosis of cultured human retinal pigment epithelial (RPE) cells exposed to visible light,and determine the role of bFGF, fibroblast growth factor receptor 1 (FGFR1),bcl 2 and caspase 3. Methods (2000? 500) lx cold white light was used. Exogenous bFGF was utilized during culture. Annexin annexin V fluorescein isothiocyanate/propidium iodium (V FITC/PI) labeling,flow cytometry, Immunocytochemical staining, enzyme associated absorb examing and reverse transcriptional polymerase chain reaction (RT PCR) were used to determine the apoptosis, the expression levels of bFGF, FGFR1, bcl 2, as well as the activity of caspase 3. Results No protective effect of bFGF was observed under the concentration 5 ng/ml. A significant inhibition of apoptosis was found in 10 ng/ml and 20 ng/ml groups ( P5 ng/ml) groups than light exposure groups ( P

Objective To investigate the pattern and the role of cytoskeletal genes expression during nerve regeneration. Methods We examined the changes of expression of ? tubulin(? Tub)and three neurofilament(NF)subunits mRNA in rat corticospinal neurons of sensorimotor cortex on the 1st,3rd,5th,7th,10th,14th,21st,28th and 56th days after spinal cord semitransection injury by using in situ hybridization. Results Levels of ? Tub,NF L,NF M and NF H mRNA were dramatically downregulated within large sized pyramidal neurons in layer V of the sensorimotor cortex of side lateral after spinal cord injury.The level of ? Tub mRNA was decreased as early as 1 day postinjury,whereas the levels of NF subunits mRNA were not decreased until later time(on the 3rd days postinjury),and both of them did not return to control levels on the 56th days following the lesion. Conclusion ? Tub gene expression if inhibited in CNS neurons postinjury. The axotomy signeal and the continuance downregulation of NF mRNA expressioon following spinal cord injury may contribute to the ineffective regeneration response of CNS neurons.

Objective In order to explore the mechanisms that regulate the expression of genes coding for cytoskeletal proteins during nerve regeneration. Methods In the present study, in situ hybridization was used to examine the changes of light(NF L),medium(NF M)and heavy (NF H)neurofilament protein subunits mRNA in L 4-6 dosal root ganglion (DRG) sensory neurons during nerve regeneration following a unilateral crush of the sciatic nerve. Results The hybridization signals of each neurofilament subunit mRNA were dramatic decrees in DRG sensory neurons post axotomy by light microscope.The signals of NF\|L and NF\|M mRNA were located in cytoplasm of neurons,whereas NF H mRNA was found in both nucleus and cytoplasm of neurons. Conclusion There are the different mechanisms of regulation of neurofilament subunit genes expression,the reduced neurofilament gene expression may represent a general response to axonal injury and plays an important role in effective nerve regeneration.

Objective Through detect the expression of DLC1 and RhoA protein in breast cancer tissues ,investigate the effect of DLC1 and RhoA in lymphatic metastasis of breast cancer .Methods Totally 25 breast cancer specimens that had occurred lymph node metastasis and 30 breast cancer specimens that had not occurred lymph node metastasis were collected ,the expression of DLC1 and RhoA were tested by immunochemistry ,and the experimental data were analyzed by statistical methods .Results The positive rate of DLC1 in the breast cancer specimens that had occurred lymph node metastasis was 44 .00% ,that had not occurred lymph node metastasis was 73 .33% (χ2 =4 .889 ,P=0 .027) ,and the positive rates of RhoA in the two specimens were 92 .00%and 50 .00% (χ2 =11 .264 ,P=0 .001) .Spearman rank correlation analysis showed the expression of DLC 1 and RhoA had a nega‐tive correlation in breast cancer tissues (r= -0 .454 ,P=0 .000) .Conclusion The breast cancer lymphatic metastasis and is closely related to the low expression of DLC1 and the high expression of RhoA .

We investigated molecular identification of a group of 14 strains of Aeromonas sp .,and genetic background of re‐sistance to beta‐lactams ,aminoglycosides .From January to December 2012 ,14 strains of Aeromonas sp .were collected from stool from diarrheal patients in enteric clinics in Ningbo First Hospital in Zhejiang Province ,China .Then ,molecular identifica‐tion by 16SrDNA ,23 kinds of beta‐lactamase genes ,6 kinds of aminoglycoside modifying enzyme genes ,6 kinds of 16srRNA methylase genes ,and 6 kinds of mobile genetic elements were analyzed by PCR .In addition ,genotyping and sample cluster a‐nalysis were performed .Results showed that 10 strains of A .hydrophila ,1 strain of A .aquariorum ,A .sobria ,A .entero‐pelogenes ,A .punctata were confirmed by 16SrDNA sequencing and arithmetic .Five kinds of beta‐lactamase genes ,4 kinds of aminoglycoside modifying enzyme genes ,and 3 kinds of mobile genetic elements were positive .BlaAQU of strain No .4(AQU‐2) and strain No .11(AQU‐3) were new subtypes .It’s suggested that identification of Aeromonas sp .should be performed by molecular identification method .This group of 14 strains of Aeromonas sp .conferred multidrug resistance .

In recent years,nuclear medical imaging has become a routine examining technology.In general,angiographic medicine has to be injected into human body.Considering the short-coming of the manual injection,it seriously needs a injecting pump with the functions such as auto-control,push-pull injecting,synchronous injection of two injectors.

Objective To discuss the value and the effect of radiofrequency treatment of tennis elbow under the arthroscopy. Methods From March 2003 to Jan 2004,eight cases of tennis elbow were treated by TOPAZ with local anesthesia under arthroscopy. Results The effects and functions were satisfactory after radiofrequency treatment. The symptoms of pain were completely disappeared in 7 and obviously relieve in 1. No blood vessel and nerve injury or infection occurred. Conclusion The methods of radiofrequency therapy for tennis elbow under arthroscopy has the advantage of minimum invasion, less pain and earlier rehabilitation.