By discussion the experience of surgery teaching for foreign students by the methods of CBL+MCAI, the successful teaching experience are the followings: making sufficient preparations before lesson according to the features and difficulties in the foreign students' surgery teaching; Emphasis on CBL teaching methods and rational application of multimedia computer assisted instruction,strengthening the teaching disciphne of foreign students and the cultivation of the spirit of students' active learning.These measures stimulated the enthusiasm of students' active learning and improved the quality of teaching.

Purpose:To explore the clinical significance of gene diagnosis in detecting micrometastases in lymph node,peripheral blood and bone marrow from non-small-cell lung cancer patients and the correlation to e ach other. Methods:The MUC1 mRNA expression was detected in lymph nodes, p eripheral blood and bone marrow from 31 lung cancer putients and 10 benign p?lm onary lesion patients by nested RT-PCR. Results:The sensitivity of the the RT-PCR technique was 10 -6 in this study. The positive rates of MUC1 mRNA were 54.6%, 32.3% and 22.6% in lymph nodes , peripheral blood and bone marrow from lung cancer patients resp ectively , and highly correlation existed among the three groups(P

Objective:By measuring the expression of nuclear factor of ?B(NF-?B) and apoptosis in the tissue of benign and malignant pancreatic diseases.Methods:The expression of NF-?B factor was measured by immunohistochemical method to measure.The expression of apoptosis for cells was determined by TUNEL method.Results: When measuring the expression of NF-?B p65 protein in the tissue,we found firstly,in the tissue of pancreatic cancer,NF-?B p65 protein was located in the nucleolus and cytoplasm,the number of positive cell was obviously more than that in the control group(P

Accordingtothe analysis ofthe characters and difficulties ofskill ofanimal surgery,we divide the teaching process into three stages:theory classes,aseptic technique and basic operating techniques,animal practice.PBL+LBL double-track teaching methods is performed,which can make up the defect of PBL in practice and should be advocated and spread in the teaching of skill of animal surgery.

Purpose　To detect the prevention of EGF and GLN on the side effect of TPN.　Methods　We randomized SD rats into four groups:group A (control),B(STPN),C(TPN+GLN),D(TPN+GLN+EGF).All rats were maintained on their respective diets for 8 days,at which time they were killed and the proximal jejunum was incised for biopsy,the MLN and celiac vein blood were collected for bacterial culture(aerobic culture).　Results　Group B rat mucosal cell atrophied,DNA and RNA content and plasma cell decreased,the positive rat of MLN bacterial culture was 62.5%.Atrophy in group C alleviated and bacterial translocation rate declined(37.5%).Group D was similar with group A.　Conclusions　GLN combined with EGF is more effective in protecting gut barrier function and preventing bacterial translocation than GLN alone.

Objective To study the relationship between plasmic nitric oxide(NO),endothelin-1(ET-1) levels and occurrence of hepatopulmonary syndrome(HPS) in liver cirrhosis with portal hypertension(LCPH) patients.Methods Fifty-two LCPH patients were divided into group Ⅰ(with HPS) and groupⅡ(without HPS).Plasmic NO and ET-1 levels were detected by nitric acid reductase and radioimmunoassay(RIA) respectively.Results Occurrence of HPS in all patients was 11.5%(6/52),and was 30%(3/10) in Child-Pugh class C patients.In group Ⅰ,50%(3/6) patients were in Child-Pugh class C.The comparison showed that plasmic NO levels were significantly higher in group Ⅰ than that in group Ⅱ[(110.40?28.02)?mol/L vs.(79.56?22.12)?mol/L,P0.05).Conclusions Elevated plasmic NO levels is an important characteristic of HPS,and,when combined with Child-Pugh classification,it can be significant for diagnosis of HPS in LCPH patients.

Objective To investigate the adhesion mechanism of Lactobacillus acidophilus FN001外to Peyer's patches. Methods Adhesion of L. acidophilus FN001 to mice Peyer's patches was studied in vitro using a fluorescent quantization method. The nature of adhesion mediator was studied by the effects of physical, chemical and enzymatic pre-treatments of the bacteria on their adhesion and effect of sugars on in- hibition of adhesion. The presence of lectin-like proteins in the cell surface was determined by hemagglutina- tion. Effect of L. acidophilus FN001 on inhibition of adhesion of pathogens to Peyer's patches was also stud- ied. Results The adhesion of L. acidophilus FN001 was strongly inhibited in the presence of D-mannose and methyl-ct-D-mannoside. Pretreatment of L. acidophilus FN001 with pepsin and trypsin decreased the ad- hesive capacity indicating that cell surface proteins are involved in adhesion to Peyer's patches. L. acidophi- lus FN001 could agglutinate rabbit red cell in mannose specific manner and protease pretreatment could de-crease hemagglutinin, suggesting that L. acidophilus FN001 has mannose specific lectin (s). In adherence inhibition assay, L. acidophilus NF001 could significantly inhibit adhesion of E. coli ATCC25922 to Peyer's patches when L. acidophilus NF001 were applied to Peyer's patches first or at the same time with pathogen. Conclusion It was concluded that a mannose-specific protein mediated adhesion of L. acidophilus FN001 to the Peyer's patches, and L. acidophilus FN001 could inhibit adhesion of pathogen with similar lectins speci- ficity to Peyer's patches.

PurposeTo explore the relationship between expression of apoptosis-modulating proteins and chemotherapeutic efficacy in acute leukemia. MethodsImmunocytochemical method was used to detect the expression of Bcl-2、Bcl-XL、Bax and Bak in 36 cases of acute leukemia including previously untreated/ drug-sensitive group and refractory/relapse group. ResultsThe average positive cell rates of Bcl-2 and Bcl-XL in refractory/relapse group were (41.68 ± 14.39) % and (35.96 ± 9.95 ) %, while the rates in previously untreated/drug-sensitive group were (15.64 ± 8.51 )% and (12.91 ± 8.63 )%. Statistical analysis showed the average positive cell rates of Bcl-2 and Bcl-XL in refractory/relapse group were higher than those in previously untreated/drug-sensitive group (P ＜ 0.01 ). There was no significant difference in average positive cell rates of Bax and Bak between refractory/relapse group (25.28 ± 15.49) %, (15.53 ± 10.64) % and previously untreated/drug-sensitive group (21.55 ± 12.58)%, (13.23 ± 8.36)%. The Logistic regression of expression of Bd-2 、Bcl-XL、Bax and Bak to complete remission rate (CR) of 36 cases of acute leukemia showed that Bcl-XL was the most risk factor in reducing the CR.ConclusionsBcl-2 and Bcl-XL might play important roles in multi-drug resistance of acute leukemia and Bcl-XL was more important than Bcl-2.