[Objective] To discuss the clinical effect of nasal intestinal obstruction(IO) conduct catheterization on IO after ovarian cancer operation. [Method] Choose 25 cases of IO after ovarian cancer operation, randomly divide them into treatment group 10 cases and control one 15 cases. The control group take general nasogastric tube imbedding, the treatment group take nasal IO conduct catheterization;observe their post-operational complications and clinical cure effect. [Result] On complications in control group, 8 cases had electrolyte disturbance, with complication rate 53.33%, the part of clinical symptoms was re-lieved, the IO signs did not disappear or were reduced partly with X-ray;5 cases were cured clinical y, 3 had marked effect, 3 were effective, 4 had no effect, the total effective rate was 73.33%;for the treatment group, al cases were successful y imbedded and made decompression and drainage, there ’s no severe side effects after imbedding tube, the IO symptoms were effectively relieved;1 case was transferred to operation;6 cases were cured clinical y, 3 had marked effect, 1 was effective, 0 had no effect, the total effective rate was 100%;after cathetering, there ’s no severe complications, among which, 2 cases had electrolyte dis-turbance, the post-operation complication rate was 20%. The comparison of complication rate and clinical effective rate of both groups had difference of sta-tistical meaning. [Conclusion] Nasal IO conduct imbedding treating IO after ovarian cancer operation had more effect on relieving IO symptoms and reduc-ing complications than nasogastric tube.

Objective To investigate the expression patterns of osteopontin (OPN) , vascular endothelial growth factor-A (VEGF-A) , and hypoxia-inducible factor-1α (HIF-1α) in primary hepatocellular carcinoma (HCC) and determine the clinical significance of this differential expression profile.Methods Immunohistochemical staining of OPN, VEGF-A, and HIF-1α was carried out on primary HCC tissues from 90 patients, HCC-adjacent cirrhosis tissues from 20 of those patients, and normal liver tissues from 15 healthy controls.Correlations between expression levels and HCC clinicopathological characteristics were assessed by Spearman's correlation coefficient.Results The majority of HCC tissues showed positive immunostaining for OPN (69/90, 76.67%) , VEGF-A (64/90, 71.11%) , and HIF-1α (66/90, 73.33%) .OPN-and VEGF-A-positivity were significantly higher than the results from the cirrhosis tissues and normal tissues.HIF-1α-positivity was similar between the HCC and cirrhosis tissues, but both were significantly different from the normal tissues.The differential expressions of OPN, VEGF-A, and HIF-1α were significantly correlated with tumor thrombus, capsular integrity, tumor differentiation and stage, and metastasis (P<0.05) .Conclusion HCC tissues overexpress OPN, VEGF-A, and HIF-1α and this differential profile may be related to HCC progression.Future investigations of this triad of factors may provide novel insights into the biological characteristics of HCC and reveal important targets of molecular therapy.

Humans ; Thyroid Neoplasms ; pathology ; therapy

Objective: To improve the diagnosis and treatment level of secretory otitis media (SOM) of children. Method :Clinical information of 80 children with SOM were colletcted and analysed. All of the 80 cases (144 ears)were treated by myringotomy with Grommet insertion. Among them, 30 cases by adenotonsillectomy. 10 cases by palatoplasty. Secretions of 54 ears were carded out by bacterial culture. Result:80 cases were followed up for 6 to 12 mothes. 120 ears of 144 were cured (83％) ,with hearing improved. The tympanograms and hearing threshold of pure tone were found to be normal. The Gram＇s positive and negative germs of bactevial culture were 20.7％ (6/29 samples ) and 24％ (6/25 samples ) respectively. Conclusion: Children with secretory otitis media should be treated as early as posible to improve the patients＇ hearing and develope their speech.

Background Studies showed that traumatic optic neuropathy (TON) can activate and induce immuno-inflammatory response,while T helper cell 17 (Th17), a CD4+ T lymphocyte, is associated with immunoinflammatory response.However,the effects of Th17 on the development of TON are unclear.Objective This study was to observe the changes of Th17 lymphocytes in spleen and the expression of interleukin-17 (IL-7) in retina in TON rats.Methods Seventy male 4-week-old SD rats were randomly divided into normal control group, sham operation group and TON 1-, 3-,7-, 14-and 28-day group according to random number table.The optical nerves of the right eyes were exposed and impacted at a 25° angle by using fluid percussion injury (FPI) device with the average force (699.1±60.8) kPa to create the TON models in the TON groups,and only optical nerves were exposed in the sham operation group.Flash visual evoked potential (F-VEP) was recorded and the amplitude and latency were measured to evaluate the function of optical nerves of the rats.The rat splenocyte suspension in various groups was prepared for the detection of Th17 cells by flow cytometry, and the expression of IL-17 protein was detected by immunochemistry.Results No significant differences were found in the amplitudes and latencies of P2 wave, proportion of Th17 cells and the IL-17 positive cell numbers in retina between the normal control group and sham operation group (P =0.829,0.830,0.856,0.496).The amplitudes of P2 wave were significantly lower, and the latencies of P2 wave were significantly longer in the TON groups than those in the sham operation group (all at P＜ 0.05).The proportions of Th17 positive CD4+ T lymphocytes were (0.94±0.13)％,(1.80±0.18)％,(1.98± 0.20)％ ,(2.34±0.20)％ ,(2.11±0.13)％ ,(1.92±0.18)％ in the sham operation group,TON 1-day group,TON 3-day group,TON 7-day group,TON 14-day group and TON 28-day group, respectively, showing significant differences between the sham operation group and various TON groups (all at P＜0.05).In addition, the IL-17 positive cell numbers in retina were 796.326 ± 100.028, 1 673.416 ± 188.021,1 892.431 ± 151.026,2 420.454 ± 256.024,1 996.429±177.022 and 1 629.410±127.023 in the sham operation group,TON 1-day group,TON 3-day group,TON 7-day group,TON 14-day group and TON 28-day group, respectively, showing significant differences between the sham operation group and various TON groups (all at P＜0.05).Conclusions In rats of TON, the proportion of Th17 cell in splenocytes and the expressions of IL-17 in retina are increased,suggesting that Th17 and IL-17 participate in the progression of TON.

This study was carried out to determine the effects of mild iron deficiency anemia (IDA) on physical work capacity (PWC) of 7-year-old children in Beijing. Ninety-three mild IDA children and thirty-seven well-to-do children served as volunteer subjects. Finger blood samples were obtained for Hb, FEP, FEP/Hb, SF determination; PWC data were assessed by measuring PWC 170, R.PWC170, Workload Heart Rates (W.HR), estimated Vo2max before, in the middle, and after treatment, respectively. After the mild IDA children were cured with iron supplement (300mg ferrous sulfate per day) for 60 days, their values of Hb, FEP, FEP/Hb, SF changed significantly from original 10.5g/dl, 41.8ng/dl, 4.6?g/g, 13.2ng/ml to 12.5g/dl, 24.1?g/dl, 2.0?g/g, 75.7ng/ml respectively (P

Thirty two rabbits were divided into 4 groups: (1) Normal diet control group, ( 2 ) Hyperlipid diet control group, ( 3 ) Hyperlipid diet group injected with 30 mg/kg GSL, ( 4 ) Hyperlipid diet group injected with 60 mg/kg GSL, The whole expeiment took about 42 days. On 24d all hyperlipid diet were removed, and only normal diet given. At the end of this experiment all rabbits were killed by bleeding and the results showed that GSL could decrease the serum lipids GSL could increase the HDL - C in the rabbits injected with 60 mg/kg GSL. GSL could protect the walls of aortas from the damage of hyperlipid serum. The contents of lipid and MDA in the Walls of aortas were decreased. GSL had the effects of protection on the heart and liver by decreasing of Total Cholesterol and MDA. So we conclude that GSL has the effects of lipid regulation and lipid antipeoxidation on the chronic hy-perjipidemic rabits.

Objective. To investigate the mechanism of tazarotene in active psoriasis vulgaris. Methods. HB-EGF protein in active psoriatic lesions before and 10 days after the treatment of tazarotene was detected by immunohistochemistry. Results. There was nearly no expression of HB-EGF in psoriatic lesions (9.1%), but after the treatment by tazarotene, there was expression of HB-EGF not only in basal layer (95.5%), but also focal expression in suprabasal layers of epidermis (77.3%). Conclusion. Tazarotene inhibit proliferation and induce apoptosis of keratinocytes through upregulating expression of HB-EGF in psoriatic epidermis.

Analysising of the students' role will contribute to improve the quality of clinical teaching.The core of the students' role is “student”,they are the principal part of the learning activities.At the same time,students have on the shoulders the practice role of clinical nurse.The remarkable thing is that the students have kinds of needs of different levels,they should be regarded as the holistic person who have the individual otherness and commonness and the inimitable value.The characteristic of students can influence their behaviors in clinical activities.

Rehabilitation treatment after cerebral infarction establishes on the basis of plastic changes in the central nervous system,which can compensate the functions lost in the affected brain regions.Neurorehabilitation training realizes neurological function recovery partly by enhancing cortical reorganization.Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive approach that can effectively improve the cortical excitability,This article reviews the application of rTMS in the clinical studies of motor function recovery in patients with hemiplegia after cerebral infarction.