[Objective] To discuss the clinical effect of nasal intestinal obstruction(IO) conduct catheterization on IO after ovarian cancer operation. [Method] Choose 25 cases of IO after ovarian cancer operation, randomly divide them into treatment group 10 cases and control one 15 cases. The control group take general nasogastric tube imbedding, the treatment group take nasal IO conduct catheterization;observe their post-operational complications and clinical cure effect. [Result] On complications in control group, 8 cases had electrolyte disturbance, with complication rate 53.33%, the part of clinical symptoms was re-lieved, the IO signs did not disappear or were reduced partly with X-ray;5 cases were cured clinical y, 3 had marked effect, 3 were effective, 4 had no effect, the total effective rate was 73.33%;for the treatment group, al cases were successful y imbedded and made decompression and drainage, there ’s no severe side effects after imbedding tube, the IO symptoms were effectively relieved;1 case was transferred to operation;6 cases were cured clinical y, 3 had marked effect, 1 was effective, 0 had no effect, the total effective rate was 100%;after cathetering, there ’s no severe complications, among which, 2 cases had electrolyte dis-turbance, the post-operation complication rate was 20%. The comparison of complication rate and clinical effective rate of both groups had difference of sta-tistical meaning. [Conclusion] Nasal IO conduct imbedding treating IO after ovarian cancer operation had more effect on relieving IO symptoms and reduc-ing complications than nasogastric tube.

Objective To investigate the expression patterns of osteopontin (OPN) , vascular endothelial growth factor-A (VEGF-A) , and hypoxia-inducible factor-1α (HIF-1α) in primary hepatocellular carcinoma (HCC) and determine the clinical significance of this differential expression profile.Methods Immunohistochemical staining of OPN, VEGF-A, and HIF-1α was carried out on primary HCC tissues from 90 patients, HCC-adjacent cirrhosis tissues from 20 of those patients, and normal liver tissues from 15 healthy controls.Correlations between expression levels and HCC clinicopathological characteristics were assessed by Spearman's correlation coefficient.Results The majority of HCC tissues showed positive immunostaining for OPN (69/90, 76.67%) , VEGF-A (64/90, 71.11%) , and HIF-1α (66/90, 73.33%) .OPN-and VEGF-A-positivity were significantly higher than the results from the cirrhosis tissues and normal tissues.HIF-1α-positivity was similar between the HCC and cirrhosis tissues, but both were significantly different from the normal tissues.The differential expressions of OPN, VEGF-A, and HIF-1α were significantly correlated with tumor thrombus, capsular integrity, tumor differentiation and stage, and metastasis (P<0.05) .Conclusion HCC tissues overexpress OPN, VEGF-A, and HIF-1α and this differential profile may be related to HCC progression.Future investigations of this triad of factors may provide novel insights into the biological characteristics of HCC and reveal important targets of molecular therapy.

Humans ; Thyroid Neoplasms ; pathology ; therapy

Objective To investigate the expression of uPAR and VEGF-C in hepatocellular carcinoma (HCC) and its effect on the biological behavior of HCC. Methods The expression of uPAR and VEGF-C in liver tissue specimen of 33 patients with HCC was detected by immunohistochemical staining. Results The positive expression of uPAR and VEGF-C in HCC tissues was located in cytoplasma of cancer cells. The positive rates of uPAR and VEGF-C were 69.7 %(23/33) and 78.8 %(26/33) respectively in 33 cases of HCC. The expression rates of uPAR and VEGF-C were significantly higher in HCC with metastasis than in HCC without metastasis(P<0.05). But there were not significant correlation between the expression of uPAR or VEGF-C and tumor size, tumor grade, AFP level, hepatic cirrhosis. There was a close correlation between uPAR and VEGF-C in HCC(r=0.676, P<0.01). Conclusion The expression of uPAR and VEGF-C is correlated with metastasis, uPAR and VEGF-C may be signs of aggressive phenotype for evaluating treatment and prognosis of HCC. Examination of these two proteins may be helpful to .judge biological behavior of HCC.

OBJECTIVE: To study the absorption and transport of pachymic acid (PA) isolated from the sclerotium of Poria cocos (Schw.) Wolf. in human intestinal epithelium. METHODS: By using Caco-2 (the human colonic adenocarcinoma cell lines) cell monolayers as an intestinal epithelial cell model, the permeability of PA was studied from apical side (AP side) to basolateral side (BL side) or from BL side to AP side. The PA was measured by reversed-phase high performance liquid chromatography coupled with UV detector at maximum absorption wavelength of 210 nm. Transport parameters and apparent permeability coefficients (Papp) were then calculated and compared with those of propranolol and atenolol, which were the transcellular transport markers for high and poor permeability respectively. RESULTS: The Papp values of PA were (9.50+/-2.20) 10(-7) cm/s from AP side to BL side, and (11.30+/-5.90) 10(-7) cm/s from BL side to AP side, respectively. Under the condition of this experiment, the Papp values were 1.45x10(-5) cm/s for propranolol and 4.22x10(-7)cm/s for atenolol. CONCLUSION: PA is transported through the Caco-2 cell monolayer in a concentration-dependent manner and the transport was linear with time. The absorption in apical to basolateral direction and secretion in basolateral to apical direction were poor and their Papp values were comparable to atenolol. Besides passive diffusion of PA, ATP is partially involved in its transport.

Objective: To improve the diagnosis and treatment level of secretory otitis media (SOM) of children. Method :Clinical information of 80 children with SOM were colletcted and analysed. All of the 80 cases (144 ears)were treated by myringotomy with Grommet insertion. Among them, 30 cases by adenotonsillectomy. 10 cases by palatoplasty. Secretions of 54 ears were carded out by bacterial culture. Result:80 cases were followed up for 6 to 12 mothes. 120 ears of 144 were cured (83％) ,with hearing improved. The tympanograms and hearing threshold of pure tone were found to be normal. The Gram＇s positive and negative germs of bactevial culture were 20.7％ (6/29 samples ) and 24％ (6/25 samples ) respectively. Conclusion: Children with secretory otitis media should be treated as early as posible to improve the patients＇ hearing and develope their speech.

Objective To analyze the distribution rule of TCM syndromes and syndrome elements in chronic atrophic gastritis with osteoporosis by retrospective study method, and provide evidence for TCM treatment. Methods TCM symptoms, syndromes and syndrome elements of 127 cases of chronic atrophic gastritis with osteoporosis were retrospectively investigated and statistically analyzed. Results The distribution of TCM syndromes in chronic atrophic gastritis with osteoporosis was as follows:there were 51 cases of asthenia syndrome of both qi and yin, accounted for 40.16%;28 cases of yang deficiency syndrome of spleen and kidney, accounted for 22.05%;21 cases of syndrome of qi deficiency and blood stasis, accounted for 16.54%. The distribution of syndrome elements was:there were 82 cases of qi deficiency, accounted for 64.57%;61 cases of yin deficiency, accounted for 48.03%;28 cases of yang deficiency, accounted for 22.05%;21 cases of blood stasis, accounted for 16.54%;12 cases of qi stagnation, accounted for 9.54%;6 cases of dampness-heat, accounted for 4.72%. Conclusion The basic pathogenesis of chronic atrophic gastritis with osteoporosis is deficiency of both spleen and kidney. Deficiency of kidney is mainly qi deficiency, yin deficiency, yang deficiency, or even deficiency of both yin and yang. Reinforcing spleen and kidney is the important principle of treatment.

Objective To assess stress degree of osteosarcoma caregivers and explore the impact factors of stress response. Methods We used CRA scale to investigate stress state of the primary caregivers of osteosarcoma patients, the demographic characteristics, status of patients, the objective amount of care and scores for each latitude underwent multiple regression analysis. Results The pressure of caregivers from the city was relatively light, annual income more than 50 thousand yuan and with help from the families to take care patients could effectively alleviate the pressure. The caregivers came from rural areas, household income less than 20 thousand yuan, caregivers were the patients' parents, had their own job presently, with no one to help were high - pressure groups. Conclusions Caregivers of osteosarcoma patients with high-pressure need focused intervention, more help and support should be given to these population.

This study was carried out to determine the effects of mild iron deficiency anemia (IDA) on physical work capacity (PWC) of 7-year-old children in Beijing. Ninety-three mild IDA children and thirty-seven well-to-do children served as volunteer subjects. Finger blood samples were obtained for Hb, FEP, FEP/Hb, SF determination; PWC data were assessed by measuring PWC 170, R.PWC170, Workload Heart Rates (W.HR), estimated Vo2max before, in the middle, and after treatment, respectively. After the mild IDA children were cured with iron supplement (300mg ferrous sulfate per day) for 60 days, their values of Hb, FEP, FEP/Hb, SF changed significantly from original 10.5g/dl, 41.8ng/dl, 4.6?g/g, 13.2ng/ml to 12.5g/dl, 24.1?g/dl, 2.0?g/g, 75.7ng/ml respectively (P

Thirty two rabbits were divided into 4 groups: (1) Normal diet control group, ( 2 ) Hyperlipid diet control group, ( 3 ) Hyperlipid diet group injected with 30 mg/kg GSL, ( 4 ) Hyperlipid diet group injected with 60 mg/kg GSL, The whole expeiment took about 42 days. On 24d all hyperlipid diet were removed, and only normal diet given. At the end of this experiment all rabbits were killed by bleeding and the results showed that GSL could decrease the serum lipids GSL could increase the HDL - C in the rabbits injected with 60 mg/kg GSL. GSL could protect the walls of aortas from the damage of hyperlipid serum. The contents of lipid and MDA in the Walls of aortas were decreased. GSL had the effects of protection on the heart and liver by decreasing of Total Cholesterol and MDA. So we conclude that GSL has the effects of lipid regulation and lipid antipeoxidation on the chronic hy-perjipidemic rabits.