OBJECTIVE:To investigate the protective effect of trimetazidine against myocarditis induced by coxsackie virus B3(CVB3)in mice.METHODS:Myocarditis in Balb/c mice was induced by CVB3.Then the mice were randomized to blank group,myocarditis control group and trimetazidine(low,medium,and high concentrations)treatment groups for 7 days.Serum levels of IFN-?,TNF-?,IL-10 and IL-12 were determined on mice,meanwhile the virus titer of the myocardium, pathologic change,MDA concentration,SOD and CAT activities were detected.RESULTS:As compared with myocarditis control group,in groups treated with trimetazidine,serum levels of IFN-?,IL-10 and IL-12 increased significantly, but the change in serum TNF-?level was nonsignificant,the virus titer of myocardium decreased and pathological lesions of myocardium were ameliorated,the MDA concentration decreased,but SOD and CAT activities were up-regulated (P

18F-fluorodeoxyglucos (18F-FDG) PET/CT can provide metabolic information for three dimensional conformal radiation therapy planning. The biological target volume may be influenced by many factors. Studies of target delineation using 18F-FDG PET/CT and methods to improve the misalignment of PET and CT images due to breathing were reviewed in this article.

Ischemic stroke causes varying degrees of cognitive impairment, and its incidence is very high. Early diagnosis of cognitive impairment may provide the best intervention treatment opportunity, and slow disease progression. Therefore, it has attracted more and more attention. This article reviews the advances in etiology, pathogenesis, risk factors, diagnosis, prevention and treatment of cognitive impairment after ischemic stroke.

Objective To evaluate the ventricular systolic and diastolic synchronicity in elderly patients with the complete right bundle branch block (CRBBB) by Doppler tissue velocity imaging (TVI) technology. Methods The 35 elderly CRBBB patients were selected as CRBBB group, while 31 healthy people were chosen as control group to evaluate myocardial function. The time to peak velocity at systolic and early diastolic phase (Ts and Te) in the base and interlude 14 segments of left and right ventricles was measured. The average time of Ts in right ventricular 2 segments(Ts-2-RV), Ts and Te in left ventricular 12 segments (Ts-12-LV and Te-12-LV), and the standard deviation in left ventricular 12 segments (Ts-12 SD and Te-12-SD), the maximum Ts and Te difference (Ts-diff and To-dill) were calculated. The left ventricular end-systolic and end-diastolic diameter (LVEDs and LVEDd) and volume (LVEVs and LVEVd) were measured. Results (1)The time to peak velocity at systolic phase in 14 segments of left and right ventricles was longer in CRBBB group than that in control group(P<0.05 or P<0.01). Compared with the control group, the Ts-12-LV,Ts-12-SD and Ts-diff showed significant prolongation (P<0.01). The Ts-2-RV was longer than Ts-12-LV in CRBBB group[(226.3±37.4 ) ms vs. (195.5±69.5) ms, P < 0.05]. (2) There was no significant difference in the time to peak velocity at early diastolic phase in 14 segments between CRBBB group and control group(P>0.05), but the Te-12-SD and Te-diff was longer in CRBBB group than that in control group (P< 0.01). (3) The LVEDs and LVEDd were obviously increased in CRBBB group compared with controls (P<0.05 and P<0.01), but no significant difference was showed in LVEVs and LVEVd(P>0.05). Conclusions Compared with normal people, the Ts at systolic phase is longer, especially in the right ventricle, the LVEDs and LVEDd are significantly increased, and the ventricular systolic and diastolic synchronicity is not well in CRBBB patients.

Objective To observe the effects of Lipopolysaccharide (LPS) in different concentrations on expression of Robo4 in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and randomly divided into 3 groups: control group, low dose LPS group (10 μg/mL) and high dose LPS group (100 μg/mL). Robo4 protein level was detected by immunofluorescence staining and western blot , and Robo4 mRNA level was measured by real-time PCR. Results Robo4 protein and Robo4 mRNA in high dose LPS group were 0.49 ± 0.08 and 0.23 ± 0.08 respectively , which were significantly decreased than those (1.35 ± 0.15 and 0.97 ± 0.17) in control group(P < 0.05). However, there were no significant difference between the low dose (1.23 ± 0.13 and 0.94 ± 0.14) group and the control group (P > 0.05). Conclusion High dose LPS (100 μg/mL) could down-regulate expression of Robo4 in HUVECs.

Objective To observe the therapeutic effect of Xingnaojing Injection ( XI) combined western medicine for the treatment of septic encephalopathy (SE) . Methods A total of 60 SE patients were evenly randomized into treatment group and control group. Both groups were given routine western medicine therapy such as anti-inflammation, immunological and cerebral preventive treatment, or even mechanical ventilation, sub-hypothermia therapy, and insulin intensification therapy if necessary. Additionally, the treatment group was given intravenous drip of XI. The treatment for both groups lasted one week. Glasgow Coma Scale ( GCS) scores were observed before and after treatment for the evaluation of therapeutic effect of both groups. Moreover, the serum levels of C-reactive protein (CRP) and procalcitonin (PCT) , and the hospitalization fee and time in Intensive Care Unit were compared in both groups. Results ( 1) After treatment for one week, the improvement of GCS scores in the treatment group was superior to that in the control group ( P<0.05); the total effective rate was 90.0% in the treatment group and was 56.7% in the control group, the difference being significant ( P<0.05). ( 2) After treatment for 24 hours, serum CRP and PCT levels were decreased obviously in both groups (P<0.05), and the decrease in the treatment group after treatment for 24, 48, and 72 hours and for one week was superior to that in the control group ( P<0.05). ( 3) Hospitalization fee was less and hospitalization time in ICU was shorter in the treatment group than those in the control group ( P<0.05). Conclusion XI shows satisfactory effect on relieving illness and increasing cure rate, and on decreasing hospitalization fee and time in ICU.

Bidirectional signal transduction is a newly elucidated mechanism in intercellular communication. The bidirectional signal transduction mediated by the Eph-ephrin is an important representative in this field. The Eph family receptor tyrosine kinases and their membrane-bound ligands, the ephrins, play pivotal roles in the development of nervous system, angiogenesis, etc. The signal transduction into cells by Eph receptors is the forward signal, whereas the signal transduction by ephrins is the reverse signal. Based on their molecular structures, the ephrins can be divided into two subclasses, i.e. ephrinA and ephrinB. The ephrinBs are transmembrane proteins, which can activate FAK, JNK and Wnt signal transduction pathways through phosphotyrosine-dependent signaling and PDZ-binding motif-dependent signaling. The ephrinAs are glycosylphosphotidylinositol (GPI) anchored proteins, which can also mediate reverse signal transduction.

Objective To explore the application of the comprehensive pain assessment scale to the treatment of postherpetic neuralgia (PHN). Methods About 100 patients suffering from PHN were randomly divided into two groups: the control group and experiment group. The former was treated with recording for pain assessment and the latter was treated with the comprehensive pain assessment scale. Two sets of data were compared and analyzed in view of quality of sleep, average length of hospitalization and the duration of side effects of drugs. Result The effect of the experiment group was better than the control group in the quality of sleep, the average length of hospitalization, reduction of side effect duration of drugs (P<0.05). Conclusion The comprehensive pain assessment scale throughout the whole process of the PHN treatment can achieve timely and effective assessment of pain and reduce the side effect duration of drugs, improve their sleep quality, and shorten the hospital stay.

Young Cadre talents in the hospital will be the most important influence factor of the future of the development of Medical care .Their training must includes management , medical skill , science study and so on and be different from the other professions .This study selected the Young Cadre talents of a hospital as the object .And we got the basic condition of the personal , training requirement and the development situation of them , then put for-ward some proposals and methods on how to foster and develop them by these methods below : literature research , questionnaire survey , field survey , statistical analysis , case analysis , interviews with 360 degree and so on .

Higher medical colleges and universities shoulder the macro guidance function,business improvement performance,qualification educational function,research and model function,medical ethics education and foreign training function in the continuing medical education;should update their concepts,enhance understandings,fully tap potentials,play school advantages,carry out various medical education,expand the development channels,and make greater contributions to medical personnel training and health care.