Objective To determine the mercury in seven kinds of algal plant drugs.Method The mercury in the samples was determined by the atomic fluorescence spectrometry after been digested by microwave.Results The detection limit of mercury was 0.045?g?L~(1).The relative standard deviation was 1.65% and the average recovery was 97.77%.Conclusion The method had the advantages of simple operation,higher precision,higher sensitivity and repeatability and was suitable for the determination of mercury in the algal plant drugs.

Objective To understand the current status of Oncomelania hupensis snails and the change rules,so as to pro?vide the evidence for formulating the snail control strategy. Methods The data of snails were collected and the factors influenc?ing the changes of snail status were investigated,and all the data were analyzed in Hailing District from 2007 to 2014. Results There were 18 environments with snails in 17 villages of 3 townships in Hailing District from 2007 to 2014. The snail area was 7.832 4 hm2. Among these environments and snail areas, 6 environments and 2.134 5 hm2, snail area were detected by the village snail investigation groups, and 12 environments and 5.697 9 hm2 snail area were detected by the township snail investigation groups. In addition, in 5 non?endemic villages, 3.126 3 hm2 snail area was found. In 2008, 2012 and 2013, a total of 2.274 0 hm2 snail area was found again. Conclusions In recent years,the snail situation rises again after schistosomiasis transmission has been interrupted. Therefore,this situation should be paid attention to.

High-consuming group is a special group which has good economic base, differmental culture and relgional belif customs. These patients alwanys need high quality medical service. How to integrate with these characteristics and make the nursing model of making patients' belife, moral ideas respcted, and rights proteded is a difficult problem. The author put forward we should rule nurse staff' s ethics and actions, provide a medical envionment from which can meet high-consuming patients physical and mental needs.

Objective To investigate the epidemiological characteristics of imported malaria in Hailing District ,Taizhou City,so as to provide the evidence for formulating the effective prevention and control strategy. Methods The network reports, questionnaires and epidemic reports of imported malaria cases in Hailing District from 2010 to 2015 were collected and analyzed retrospectively. Results From 2010 to 2015,totally 46 cases of imported malaria were reported,including 40 cases of falci?parum malaria(86.96%),4 cases of ovale malaria(8.70%),1 case of quartan malaria(2.17%)and 1 case of multi?infection of falciparum malaria and tertian malaria(2.17%). No seasonal trend of time of the disease onset was observed. All the patients were return abroad with clear residence histories in malaria epidemic areas. The average time from disease onset to definite diag?nosis was 2 d and 8 patients were definitely diagnosed within 24 h. All the 46 patients were cured. Conclusion All the malaria cases reported were imported cases in Hailing District in the last five years. Effective prevention and control measures are the key to the malaria elimination.

Cardiomyopathies ; chemically induced ; Chlorine Compounds ; poisoning ; Female ; Humans ; Middle Aged ; Oxides ; poisoning

objectives To provide the reference for the Research & Development departments of hospitals by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCl and MEDLINE.and top 100 hospitals in domestic publications in 2006.Methotis The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE.and the top 100 hospitals in domestic publications in 2006 were analyzed with bibliometric analysis.Results In 2006.the average increased ratio of publications in SCI for top 20 hospitals was 47.58%.but the number of articles in MEDUNE WaS decreased.For top 100 hospitals.the increased number of domestic publications was 71.25,the number of cited publications WaS increased 160.14.Conclusions The SCI publications from top 20 hospitals increased sharply,but publicatiOils indexed by MEDLINE decreased respectively.

Objective To study the effect of chitosan on the growth of the in vitro cultured retinal nerve cells. Methods The retinal nerve cells of SD baby rats were cultured in vitro. The effects of chitosan at 0.065%,1.25%,2.5%,5%,10%,15% on the growth of the retinal nerve cells were measured by MTT method. The blank control group was created. Results 1.25%,2.5%,5%,and 10% chitosan could obviously promote the growth of retinal nerve cells,and 10% chitosan was the optimal concentration for the growth of nerve cells. Conclusion Chitosan can effectively promote the growth of retinal nerve cells,and it is necessary to undertake the further study of Chitosan.

Objective To explore the clinical and pathological characteristics of ovarian endometriosis and malignant cases, and to improve our recognition of this disease. Methods A total of 1 289 cases of ovarian endometriosis were divided into two groups: the benign group (1 275 cases)and the malignant group (14 cases). They were analyzed on: dysmenorrhea, infertility, features of ultrasound examination,site of tumour,pathological types and complications. Results Malignancy arising from ovarian endometriosis was 1.09%. As far as degree of dysmenorrhea, size of cyst >10 cm, CA125 >500 U/L, features of ultrasound were concerned, difference between the two groups was statistically significant. Conclusions In the present study, the malignant rate of ovarian endometriosis was about 1%. Malignancy should be considered seriously when the following situations occured: change in the degree of dysmenorrhea, size of cyst larger than 10 cm, CA125 higher than 500 U/L, mamilla observed by ultrasound examination.

BACKGROUND:With the in-depth study of cancer stem cels, increasing evidence has shown that cancer stem cels are the reason for cancer metastasis and recurrence, and therefore it is of great significance to isolate and identify cancer stem cels that can elucidate the pathogenesis of cancer and development of anticancer drugs.
OBJECTIVE: To isolate and culture gastric cancer stem cels and then to detect its biological characteristics.
METHODS: Cancer tissues from 16 patients with gastric cancer were cultured using adherent method and enzymic digestion methods to isolate gastric cancer stem cels. The cel morphology was observed under inverted microscope to drawn out growth curves. Osteogenic and adipogenic ability of cels were also detected.
RESULTS AND CONCLUSION: Gastric cancer stem cels were isolated successfuly by both two methods. Under the microscope, the cels were fusiform- or polygon-shaped. At confluence, the cels grew in a vortex or radial manner. The cel growth curve showed that 1-3 days were latent period, 4-9 days were logarithmic growth phase, and over 10 days were growth plateau phase. Flow cytometry results showed that passage 3 gastric cancer stem cels highly expressed CD90, CD29, CD44, but lowly expressed CD34, CD45 and HLA-DR. After osteogenic induction, calcified nodules were clearly visible in the gastric cancer stem cels at passage 3; after adipogenic induction, bright and tiny fat droplets were seen in the cytoplasm. These findings indicate that cancer stem cels exist in the gastric cancer tissues, and have similar morphology, biological property and multipotent differentiation ability to normal cels, which are probably involved in gastric cancer occurrence and development.

Sepsis is the systemic inlfammatory response to infection and a major cause of mortality in critical patients. The severe disorder of immune system is the common pathophysiological changes in septic patients. Mesenchymal stem cells (MSCs) have shown great immunoloregulation properties in recent studies. It can increase the level of IL-10, an anti-inflammatory cytokine, promote the secretion of prostaglandin E2 (PGE2), indolamine 2,3-dioxygenase (IDO), and regulate the proliferation, differentiation and function of the immune cells such as mononuclear macrophage, t-lymphocytes, natural killer cells and so on. MSCs may provide new ideas for the treatment of sepsis.