Objective:To study the quality standard for Fujie lotion. Methods:The qualitative identification of Sophora alopecu-roides L, Cnidium monnieri ( L. ) Cuss and borneol was detected by TLC. The quantitative determination of matrine was detected by HPLC. Results:The identification by TLC was highly specific, and the content determination method was accurate and repeatable. The linear range of matrine was 0. 013 0-1. 30 mg·ml-1, and the average recovery was 97. 1% with RSD of 1. 7%(n=6). Conclu-sion:The standard can effectively control the quality of Fujie lotion.

Objective To explore the role of intracellular Ca~(2+) in inhibition of axonal outgrowth,which could learn more about the signal transduction mechanism of Nogo-A inhibiting axonal outgrowth. Methods Using laser confocal microscope,intracellular calcium level in cerebellar granule neurons after Nogo-A added in different times was measured and the effect of Nogo-A inhibiting axonal outgrowth when priming nimodipine was observed. Results The concentration of Ca~(2+) increased in 5 minutes after Nogo-A added,and reached to peak in 30 minutes,then decreased gradually,90 minutes later dropped to normal level.When priming with nimodipine,the axonal inhibition by Nogo-A was partly abrogated compared with control.Conclusion Ca~(2+) may participate in the signal transduction of Nogo-A inhibiting axonal outgrowth.

Objective To evaluate the feasibility and advantage of using withdrawable Sigma stent for the treatment of tracheo-esophageal fistular. Methods The stents were placed into trachea or/and esophagus by interventional or/and endoscopic technique. Results Esophageal cancerin 17 and benign disease in 2. Totally 38 stents were placed in 19 cases of patients (trachea 19, main bronchus 1, esophagus 18). Only one tracheal stent was placed in 2 cases. Two stents(one in trachea, another in esophagus) were placed in 15 cases ( 11 cases with 2 tubular type stent, 4 cases with one tubular and one bifurcated type stent). Three stents were placed in 2 cases. One stage placement of the stent in 35, withdrawed the stent and reinserted again in 3. All the patients have normal meal 2～4 days postoperatively. Only one patient had a little contrast in the trachea during X-ray exam but without symptoms, the fistulae completely sealed in 18 cases. Follow-up was fron 3 months to 3 year. 10 patients were still alive; the longest survival is 18 months. There were 9 deaths. The causes of death were pulmonary infection in 1, hemorrhage in 1, and systemic metastasis in 7 cases. Conclusion Sigma stent is can effectively treat tracheal or/and esophageal stenosis or fistular.

Objective To examine the reliability and validity of the meaning in life questionnaire (MLQ) in Chinese sergeants.Methods Convenience sampling was applied to collect data from 574 sergeants.Part of the data randomly selected (n=278) was conducted exploratory factor analysis and the other (n=296) was used in confirmatory factor analysis.The satisfaction with life scale(SWLS),self-rating depression scale (SDS),and selfrating anxiety scale(SAS) were administered to test the criterion-related validity.Results (1) Exploratory factor analysis vertified a 2-factor model which consisted of presence of meaning (MLQ-P) and search for meaning (MLQ-S),and their cumulative contribution rate was 61.02％.The range of factor loading was between 0.62 and 0.85.Confirmatory factor analysis confirmed the hypothesized 2-factor model (x2 =32.82,GFI =0.97,AGFI =0.94,NFI =0.96,CFI =0.98,RMSEA =0.06).(2) The internal consistencies were good for the overall scale (Cronbach's alpha =0.79) and the subscales (Cronbach's alpha =0.80 and 0.78,respectively).(3) MLQ-P score was positively correlated with SWLS (r=0.21,P＜0.01),while negatively correlated with SDS and SAS(r=-0.50,-0.43).MLQ-S had positive correlation with MLQ-P(r=0.31,P＜0.01).(4)Scores of MLQ-p and MLQ-S in sergeants were higher than college students(t=38.13,P＜0.001,t=25.07,P＜0.001).Conclusion The Chinese version of MLQ is reliable and valid for application to Chinese sergeants.

Objective To study the effect of amelogenin (AML)on the migration,adhesion and proliferation of periodontal ligament stem cells (PDLSCs).Methods STRO-1 +PDLSCs were selected by Flow cytometer.The transwell system was used to detect the effect of AML on migration of PDLSCs.Cells attachment assay was used to detect the effect of AML on adhesion of PDLSCs.MTT was used to detect the effect of AML on the proliferation of PDLSCs.Results The results of transwell showed that AML could induce the migration of PDLSCs with positive correlation.Cells attachment assay showed that the adhesion of PDLSCs was effected by AML as well,and the effect was in-creased with the extension of incubation time.Results of MTT showed that AML could promote the proliferation of PDLSCs in a dose depend-ent manner.Conclusion AML could increase the proliferation,adhesion and migration potential of PDLSCs.

Objective To explore the expression of discoidin domain receptor 1 (DDR1) in rats with pulmonary fibrosis induced by paraquat (PQ) poisoning, and its relationship with the expression of transforming growth factor-β1 (TGF-β1). Methods 120 Sprague-Dawley (SD) rats were divided into control group and 20, 40, and 80 mg/kg PQ poisoning groups (each n = 30). Pulmonary fibrosis induced by PQ poisoning model was reproduced by one time administration of 20, 40, 80 mg/kg of 20% PQ, and the rats in control group were given 4 mL normal saline. Fifteen rats in control and different doses of PQ groups were sacrificed at 7 days and 21 days after intragastric administration, and lung tissues were collected. Pulmonary fibrosis was observed after hematoxylin-eosin (HE) staining. The immune-histochemical method was used to determine the expressions of DDR1 and TGF-β1. The relationship between the expression of TGF-β1 and DDR1 was analyzed by Pearson correlation analysis. Results The rats in control group were active, and no pathological changes in lung tissue were found. The rats in PQ groups became shortness of breath, bristles, and slow reaction etc. 0.5 hours after intragastric administration. After 7 days, the lung tissue was dark red, hard texture, appearance of yellow soil fiber nodules and obsolete hemorrhage, destruction of alveolar structure. The extent of lung injury increased gradually with the time of poisoning and the increase of PQ dose. It was shown by immune-histochemical staining that the control group had only a small amount of DDR1 and TGF-β1 positive expressions; in PQ groups, there were a large number of DDR1 and TGF-β1 positive expression particles in the alveolar wall, pulmonary interstitial and alveolar cavity. It was displayed by quantitative analysis that compared with the control group, DDR1 and TGF-β1 expressions were significantly increased in 20, 40, 80 mg/kg PQ groups with time- and dose-dependent [DDR1 (integral A value): 0.221±0.014, 0.249±0.021, 0.364±0.016 vs. 0.121±0.036 at 7 days; 0.247±0.025, 0.321±0.015, 0.432±0.027 vs. 0.139±0.021 at 21 days; TGF-β1 (integral A value): 0.230±0.016, 0.265±0.015, 0.339±0.016 vs. 0.129±0.032 at 7 days; 0.248±0.011, 0.295±0.016, 0.399±0.026 vs. 0.119±0.026 at 21 days; all P < 0.05]. It was shown by Pearson correlation analysis that DDR1 expression was positively correlated with TGF-β1 expression with the increase of PQ dose and poisoning time (DDR1 with TGF-β1: r = 0.996, P < 0.000; DDR1 with PQ dose: r = 0.985, P < 0.000; DDR1 with poisoning time: r = 0.989, P < 0.000; TGF-β1 with PQ dose: r = 0.992, P < 0.000; TGF-β1 with poisoning time: r = 0.972, P < 0.000). Conclusions The expression of DDR1 in the lung tissue in PQ poisoning rats showed a time- and dose-dependent change, and it was positively correlated with TGF-β1 expression. DDR1 may be involved in the process of pulmonary fibrosis induced by PQ poisoning.

Objective Normal radiographic scaphoid lunate (SL) interval is thought to be 2 mm and abnormal SL interval is a prerequisite for diagnosing scapholunate instability. However, in the routine neutral rotation posterior anterior (PA) view of the wrist, the frequent overlapping between scaphoid and lunate precludes proper interpretation of the interval. Moreover, the normative data in Chinese population has not been reported. We developed a new but simple technique in radiological assessment to evaluate the normal SL interval in Chinese. Methods With the forearm in neutral rotation, a PA view of the wrist was taken using a mobile Xiscan. A radiolucent cushion was put underneath the hypothenar eminence so that the metacarpal arch of hand became parallel to the receiver table of the Xiscan. The normal supination posture of the wrist was thus corrected and the true SL interval was revealed. Two hundred normal wrists were examined; there were 46 female and 54 male healthy Chinese subjects, with age ranging from 18 to 80. The SL interval was determined with wrist in neutral, radially deviated and ulnarly deviated position using the cushioned neutral rotation PA view. The length of capitate, hamate and lunate were also measured to correlate with the SL interval. Results By using the standard PA view, the SL interval could not be determined in 60% of cases. Using the new assessment method, the optimal intervals were seen in all cases. The average SL interval was (2.07?0.65) mm. There was no significant gender difference. The measurement of ulnar variance was not affected by the new method. Conclusion The modified (Cushioned) PA view was the optimal radiological assessment method for true SL interval. There was wide variation of the normal value of the SL interval in Chinese population.

Objective To investigate p53 protein expression in nasopharyngeal carcinoma (NPC) and its correlation with biological behavior, p53 gene mutation and EBV infection in 43 cases of NPC. Methods Immunohistochemical staining was applied to detect p53 protein,PCR-SSCP was used to detect p53 gene mutation, and EBV infection was determined with PCR. Results The positive rate of p53 protein expression was 76 7%(33/43)in the NPC. The positive rate of p53 protein expression in stages Ⅲ and Ⅳ was significantly higher (80 6%) than that in stages Ⅰ and Ⅱ(66 6%) (P

Objective: To study the efficacy of purging the lung and removing phlegm and blood stasis method(泻肺化痰祛瘀法) on animal model of pulmonary hypertension induced by monocrotaline and explore its mechanism.Methods: The model of pulmonary hypertension induced by monocrotaline was established in rats.Sixty healthy and clean male Wistar rats were randomly divided into normal control group,model group,low and high dosage of traditional Chinese medicine(TCM) drugs for purging the lung and removing phlegm and blood stasis groups.From the 15 th day after model establishment,two dosage groups were treated with the drugs in the prescription containing the main ingredients as follows: 9 g prepared rhubarb(制大黄),(2 g)(prepared)(euphorbia) kansui(炙甘遂),15 g lepidium apetalum(葶苈子),9 g ligusticum(川芎) and 15 g(scutellaria)(黄芩) infused into the stomach once a day for 7 days.Normal control group and model group were given normal saline infused into the stomach.Mean right ventricular pressure(MRVP) and the mean(pulmonary) artery pressure(MPAP) of rats were measured by right cardiac catheterization,and the(pathological)(changes) of lung tissues were detected by hematoxylin and eosin(HE) stained sections.(Results:) The MRVP and the MPAP in high dosage group((13.37?4.22)mm Hg and(12.26?2.79) mm Hg,(1 mm Hg)=(0.133 kPa)) were lower than that in the model group((16.27?2.27) mm Hg and(14.77?(2.39) mm Hg),P

Objective The clinical and endoscopic characteristics of ischemic colitis (IC) were retrospectively reviewed. Methods 23 aged patients with IC were included; their symptoms,signs,laboratory findings and endoscopic appearances analysed. Results IC occurred most frequently in mid-aged and elders with predominant presentations as acute onset of lower abdominal pains and bloody stools.Endoscopic lesions located mainly in left colon with segmental distribution.Mucosal edema,erosion and submucosal bleeding were common pathological features.Most lesions (91％ ) looked to be nongangrenous, transient and reversible.Only 9 percent of cases turned into chronic stage. Conclusion IC should be suspected in all eldly patients with acute onset of lower abdominal pain and bloody stools.Early colonoscopy is of diagnostic significance.