Objective To develop a kinetic chromogenic quantitative method for the determination of endotoxin content in intermediate of pertussis antigen,and to verify the method so as to better control the quality of diphtheria,tetanus,and pertussis vaccine(DTP vaccine).Methods A kinetic chromogenic assay[Limulus Amebocyte Lysate(LAL)]was developed to detect the endotoxin content in the intermediate products of pertussis antigens after detoxification,and verified for the linearity,specificity,accuracy,reproducibility and intermediate precision. The quantitative detection results of kinetic chromogenic assay were compared with those of gel method.Results The absolute value of the linear correlation coefficient（|r|）of the kinetic chromogenic assay was more than 0. 99;under the maximum effective multiple dilution,the interference test recovery of the intermediate was within 50% — 200%,and pertussis toxin(PT)diluted to 10,100 and 1 000 times,filamentous hemagglutinin(FHA)diluted to 3 000,5 000 and 10 000 times,and pertussis adhesin(PRN)diluted to 50,75 and 100 times had no interference effect on the experiment after detoxification;the accuracy verification recovery rates of PT,FHA and PRN were 125%,110% and 99% respectively;and the CVs of reproducibility verification were 7. 21%,8. 31% and 5. 84%,and the CVs of intermediate precision verification were 6. 04%,16. 29% and 12. 23%,respectively.The bacterial endotoxin content of the three batches of pertussis antigen intermediates detected by kinetic chromogenic assay was consistent with that verified by gel method,both of which were less than the limit of bacterial endotoxin in the intermediates of pertussis antigen after detoxification.Conclusion The developed kinetic chromogenic assay has good linearity,specificity,accuracy and precision with accurate detection results,which can be used to detect the endotoxin content in intermediate products of component pertussis antigen after detoxification.

Humans ; Research Design ; Retrospective Studies ; Surgical Procedures, Operative

Objective: Intelligent monitoring system of medical equipment can realize the intelligent dynamic management to equipment in the hospital wireless or wired network environment. Methods: Use of communication network, intelligent call, system monitoring and other means of science and technology to design the intelligent micro device state sensor. Results:System is mainly for hospital equipment, which can capture and management of medical equipment data. When the elderly have abnormal condition, it can send information through intelligent system, through identification and location, to realize the construction of equipment state information. Conclusion: Intelligent sensor in the system application can achieve the sharing of resources; realize the whole hand or full coverage of the intelligent health network system, providing an effective guarantee for the health of the masses.

Objective To explore the occurrence and prevention and the principle of management of chylous fistula after neck dissection. Methods Retrospective research on 1750 cases of neck dissection in Henan Tumor Hospital from January 1983 to January 2005. There were 48 cases chylous fistula and 1 case chylothorax. The incidence was 2.8 %. 5 cases on the right, 44 cases on the left. 18 cases had completed radiotherapy or chemotherapy before operation. Conservative methods and surgical methods were used in the treatment. Low fat food was supplied to the patients with chylous fistula. The conservative methods was local pressure, the surgical methods was applied while maximal production of chylous exceeding 500 ml a day. Results All the patients were cured finally. The conservative method was 12.6(5~34) days, the surgical method 7.5(3~10)day. Conclusion The key to prevent chylous fistula was to band the rupture of thoracis or lymphatic duct during operation. The conservative methods could be used in patients with slight and middle chylous, when the chylous exceed 500 ml a day or the conservative methods was unavailable, the surgical methods was appropriate, it could shorten the time of tube draw.

Objective To know the indoor air pollution status in the newly decorated residential houses in Jiaozuo and to study the effect of decoration materials on the pollution. Methods To monitor formaldehyde, ammonia and benzene levels in the indoor air of 39 newly decorated rooms in Jiaozuo. Results The exceed standard rates of formaldehyde, ammonia and benzene in the indoor air were 73.6%, 84.7% and 25.8% respectively. Exceed standard rate of formaldehyde in the room which held the new furniture was the highest, 88.8%. Conclusion Decoration may cause indoor air pollution, the most part of the pollutants are formaldehyde and benzene. The level of the pollution depends on the different usages of the rooms.

Forty rats were randomly divided into four groups and intraperitoneal injection of 1%lidocaine 400mg/kg (group Ⅰ)、1%lidocaine-?-cyclodextrin 400mg/kg (group Ⅱ)、1%alkalinized lidocaine 400mg/kg(group Ⅰ)and 1% alkalinized lidocaine-?-cyclodextrin 400mg/kg(group Ⅳ) respectively. The results showed that there was a significant difference of lethal time between group Ⅰ and Ⅱ, and no significant difference between gronp Ⅲ and Ⅳ; the times of toxic and lethal effects of group Ⅲ and Ⅳ were shortened markedly as compared to group Ⅰ and Ⅱ. It is concluded that ?-cyclodextrin can postpone the release and absorbance of lidocaine,and alkalizer can weaken this action of ?—cyclodextrin.

Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.

Biomedical Research ; Child ; Humans ; Multicenter Studies as Topic ; Pediatrics

Objective To analyse the economies of scale in public hospitals in Zhejiang Province. Methods One hundred and twenty-five public hospitals in Zhejiang Province with qualification no less than class two level two were selected as sample hospitals. Douglas production function was employed to analyse the relationship between investment and output in sample hospitals between 2004 to 2006, with two types of production models established by medical service and incomes in hospitals as dependent variables. Results From 2004 to 2006, the medical service and incomes of sample hospitals were in line with economies of scale. The production increase rate was higher than investment increase rate. The medical service, incomes, total assets and number of staff in class three hospitals were higher than those in class two hospitals (P =0.000). Conclusion Economies of scale is the ultimate incentive to continuous outstretch for hospitals. Hospitals should rationally control the scale so as to avoid problems in medical service caused by overstretch.

Humans ; Medicine, Chinese Traditional ; methods ; Treatment Outcome