Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

OBJECTIVE:To develop an HPLC method for simultaneous determination of calycosin-7-O-?-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS:The sample was separated on Zorbax Eclipse XDB-C18(250 mm?4.6 mm,5 ?m)column. The mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The UV detection wavelength was set at 280 nm. RESULTS:The linear ranges of calycosin-7-O-?-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg?mL-1,0.005 0～0.300 mg?mL-1,0.004 9～0.294 mg?mL-1,0.004 6～0.276 mg?mL-1,respectively(r=0.999 9).Calycosin-7-O-?-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION:The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.

Objective　To observe the effect of mycophenolate mofetil (MMF),a new type of immunosuppressant,on the immune function in patiens with systemic lupus erythematosus (SLE).Methods　The changes of serum lL-6,IL-8,TNF-α,sIL-2,ANA,A-dsDNA and subsets of lymphocytes were observed in patients with SLE before and after treatment with MMF and CTX by ELISA,indirect immunofluorescent assay and flow cytometry,and the effect of MMF and CTX on the functions of hematopoiesis,liver and kidney were also observed.Results　Three months after treatment with MMF,the serum levels of IL-6,IL-8,TNF-α,sIL-2,ANA,and A-dsDNA in patients with SLE were significantly decreased,CD3＋,CD4＋ and CD4＋CD45RA＋ cells were significantly increased,and CD8＋,CD4＋CD45RO＋,CD8＋CD45RA＋,CD8＋CD45RO＋ cells were significantly decreased.No remarkable injury was observed on the functions of hematopoiesis, liver and kidney after treatment with MMF.Conclusion　MMF can inhibit T and B lymphocytes selectvely and is a new type of immunosuppresant to treat SLE with less toxicity and side-effect.

The 53rd ASH meeting was held in San Diego in December 2011. The meeting has received many advanced reports on the diagnosis and treatment of multiple myeloma (MM).On therapy,with the application of thalidomide, lenalidomide and bortezomib as inductive treatments, the clinical outcomeshave been improved greatly in MM patients.Pomalidomide,a new immunomodulator,can be hopefully used as a frontline medicine for MM because of its high response. Bendamustine combination with other treatments induced efficient response and good response rates in MM patients with renal insufficiency or relapsed/refactory disease. Currently autologous stem cell transplantation is still the standard care for newly diagnosed young MM patients.At this time other kinds of novel agents have entered into clinical trials and have shown a bright future of application.

Objective To evaluate the effect of heat treatment on ultraviolet B (UVB)-induced oxidative injury to human melanocytes.Methods Melanocytes were isolated from adult foreskins,and subjected to a primary culture.After 3-4 passages of subculture,the melanocytes were classified into 4 groups:control group incubated at 37 ℃,heat treatment group incubated at 42 ℃ for 1 hour,UVB group exposed to UVB irradiation at 100 mJ/cm2,combination group receiving heat treatment at 42 ℃ for 1 hour followed by UVB irradiation at 100 mJ/cm2.After three successive days of treatment,MTT assay was performed to evaluate cell viability,a biochemical method to determine the activity of superoxide dismutase (SOD) and concentration of malonaldehyde (MDA),and flow cytometry to detect intracellular reactive oxygen species (ROS) and apoptosis in melanocytes.Results The cell survival rate,apoptosis rate,SOD activity,MDA concentration and ROS level were (100 ± 6.14)％,(4.66 ± 0.58)％,(53.39 ± 8.23) U/gprot,(1.09 ± 0.32) mmol/gprot,and 1070.85 ± 42.07 in the control group respectively.UVB exposure induced a significant increase in apoptosis rate (24.14％ ± 2.90％,P ＜ 0.001),MDA concentration (1.65 ± 0.33 mmol/gprot,P ＜ 0.01) and ROS level (1416.45 ± 79.12,P＜ 0.01),but a significant decrease in cell survival rate (50.23％ ± 5.36％,P＜ 0.01)and SOD activity (31.98 ± 1 1.89 U/gprot,P ＜ 0.01) in the UVB group compared with the control group,while the heat pretreatment markedly downregulated the UVB-induced increase in apoptosis rate (14.9％ ± 1.49％,P ＜ 0.001),MDA concentration (1.10 ± 0.26 mmol/gprot) and ROS level (1033.30 ± 68.41,P＜ 0.01),as well as the decrease in cell survival rate (74.12％ ± 6.17％,P＜ 0.01) and SOD activity (51.63 ± 6.55 U/gprot,P＜ 0.01) in the combination group.Conclusion Heat treatment could protect melanocytes from UVB-induced oxidative injury.

Objective To evaluate the efficacy of trolamine cream in the prevention and treatment of acute radioactive dermatitis in patients with breast cancer after modified radical mastectomy.Methods 80 breast cancer patients who received postmastectomy chest-wall irradiation by 6-MeV electron beam were included.40 cases in the treatment group were given trolamine cream combined with the conventional nursing care during radiotherapy,while another 40 cases in the control group were treated with the conventional nursing care.Results In the treatment group,the rates of radiation dermatitis of grade 1,2 and 3 were 70.0 ％ (28/40),22.5 ％(9/40),and 7.5 ％ (3/40),respectively,while they were 52.5 ％ (21/40),32.5 ％ (13/40) and 15.0 ％ (6/40),respectively,in control group (P ＜ 0.05).Grade 1,2 and 3 radiation dermatitis appeared in treatment group when the radiation doses were (21.91±4.39) Gy,(37.43±6.50) Gy and (46.14±6.50)Gy,respectively,while in the control group,it was (12.67±2.16) Gy,(22.61±3.51) Gy,(42.71±8.11) Gy (P ＜ 0.05),respectively.With cumulative doses of 10 Gy and 25 Gy,the rates of radiation dermatitis in the treatment group were 22.5 ％ (9/40) and 47.5 ％ (19/40),respectively,while they were 40.0 ％ (16/40) and 62.5 ％ (25/40),respectively,in the control group (P ＜ 0.05).Conclusions Trolamine cream effectively alleviates radiation skin damage in breast cancer patients after modified mastectomy,thus it is suitable for use in patients with postoperative chest wall radiotherapy.

ABSTRACT:Objective To compare the prognostic value of cystatin-C (Cys-C)and creatinine (Cr)in chronic heart failure (CHF)in old people.Methods We recruited 183 old patients (>75 years)diagnosed with CHF hospitalized at the Department of Cardiology and the Department of Geriatrics of our hospital from 2010 to 2012. Venous blood was withdrawn to detect the expressions of Cys-C and Cr.After three-year follow-up,the patients’ three-year survival was recorded.Logistic regression model was used to examine the relationship of Cys-C,Cr and other risk factors with the CHF patients’mortality.ROC curve was used to compare the prognostic value of Cys-C and Cr in CHF in old people and statistical method was adopted to analyze the combined use of Cys-C and Cr for determining their value in evaluating CHF prognosis.Results At the end of the follow-up,74 (40.44%)patients died.Serum cys-C and Cr levels of the patients in death group increased compared with those in survival group (P<0.05).Multivariate logistic regression showed that age (OR=1.11,95% CI=1.04-1.19),SBP (OR=1.06,95%CI=1.01-1.10),LVEF (OR=0.89,95% CI=0.82-0.95),BNP (OR=4.74,95% CI=1.77-12.69), creatinine (OR=2.04,95% CI=1.03-4.08),and Cys-C (OR=2.97,95% CI=1.44-6.12)were the independent risk factors for the prognosis of CHF.ROC analysis showed that Cys-C was superior to creatinine in AUC value (0.71 vs.0.65;95% CI,0.64-0.78;95% CI,0.58-0.72)(P<0.001).The AUC value of Cys-C and Cr in combination was 0.73 (95% CI,0.66-0.79).Conclusion Cys-C is a stronger predictor of the prognosis of CHF patients than creatinine and the combination of the two can increase the sensitivity and specificity in evaluating the prognosis of patients with CHF.

Objective To explore the effects of the total number of medical staff working in the room and their different activities on the colony population of air bacteria in NICU. Methods To count the colony population of air bacteria under different conditions and analyze the influenced factors . Results The colony population of room air bacteria was well correlated and statistically significant with the number of medical staff working in the room(r=0.78,P

Objective To explore the effect of swallowing training on dysphagia in patients with head and neck neoplasm receiving ra-diotherapy. Methods 60 patients with head and neck neoplasm receiving radiotherapy from March, 2014 to August, 2015 were divided into intervention group (n=30) and control group (n=30) randomly. The control group received routine nursing and relevant health education, while the intervention group received swallowing training from 2 weeks before to 3 months after radiotherapy. They were assessed with Functional Oral Intake Scale and M. D. Anderson Dysphagia Inventory 1 week, 3 months after radiotherapy. Results The scores of Function-al Oral Intake Scale (Z=-3.195, P<0.01), the total score of M. D. Anderson Dysphagia Inventory and the subscores (t>4.385, P<0.01) were better in the intervention group than in the control group 1 week after radiotherapy, as well as 3 months after radiotherapy (Z=-4.436, P<0.01;t>5.361, P<0.01). Conclusion Swallowing training could improve the condition of dysphagia of head and neck neoplasm patients after radiotherapy.

Objective To investigate the coping strategies on college students facing infectious disease. Methods Interview,question-naires and Principal component analysis were used to explore the coping strategies of college students on infectious diseases. Results Col-lege students' coping strategies to infectious diseases involving seeking social support and active problem solving two principal components, in-volving 19 aspects. Conclusion As for colleges,a support platform should be built and the ability to actively solve problems urged to be strengthened for students. Among the coping strategies of College students’ toward to the infectious diseases,students choose to seek social support more than to solve the problem initiatively,indicating that gender differences and the environment may be related. The results provide a theoretical reference to public health and health education.