A new teaching model for food fermentation technology education is proposed in this paper,aiming at improving some of the drawbacks of the traditional teaching method. Some novel approaches,such as summarization,comparison,discussion,as well as bi-lingual lecturing and multi-media assisted teaching,are introduced to inspire students’ passion for learning. Meanwhile,arranging students as dedicated class speaker and organizing students to participate in the exploring experiments also reflect the technology major education characteristics of normal university.

Through the exertion of the PBL in the teaching of the medical college,the teachers exert the effect in bringing forward questions,grouping,discussion and competition.After the practice of PBL,some new methods have been put forward.

Objective To set up a method to detect the mutation of CYP2C9 1075A ＞ C and VKORC1-1639 G ＞ A in clinical practice,investigate the distribution of CYP2C9 1075A ＞ C and VKORC1-1639 G ＞ A in Beijing Han native,and evaluate the correlations between CYP2C9 1075A ＞ C,VKORC1-1639 G ＞ A and Warfarin maintenance dose,so that the results will contribute to Warfarin therapy.Methods A total of 145 Han Beijing patients received long-term Warfarin anticoagulant therapy orally in the people's hospital between May to September in 2011 were enrolled in this study.The patients suffer from atrial fibrilation,thromboembolism and heart operation respectively.The ARMS-TaqMan real time PCR technique was used to detect the mutation of CYP2C9 1075A ＞ C and VKORC1-1639 G ＞ A.The data were collected on gender,age and BSA.The correlations between stable warfarin dosage and each factor were analysised.Results The detection limit of ARMS-TaqMan real time PCR technique was 105 copies/ml in this assay.The intra-and inter-coefficients of variations (CV) were less than 5.5％ and less than 9.0％ respectively.The coincidence rate of ARMS-TaqMan real time PCR and direct sequencing was 100％ for testing 50 paralled samples.Among the 145 patients,the frequencies of CYP2C9 1075 AA,AC and CC was 93.8％ (136/145),6.2％ (9/145),0(0/145) respectively.The frequencies of VKORC1-1639 GG,GA and AA was 0.7 ％ (1/145),21.4％ (31/145),77.9％ (113/145) respectively.Allele frequencies of genotypes were in Hardy-Weinberg equilibrium.Every patients' INR was within range 2.0-3.0.Among the 145 patients,dosage requirements were higher in CYP2C9 1075 AA phenotype than that in AC type (F =0.199,P ＜0.05) and were higher in VKORC1-1639 GA phenotype than that in AA type (F=1.745,P ＜0.001).Irrespective of other factors,age,BSA,CYP2C9 1075 and VKORC1-1639 was accounted for 11.9％,12.9％,4.4％ and 16.7％ of interindividual variation of Warfarin dosage respectively.Linear stepwise regression analysis was used to identify factors contributing to Warfarin stable does.The final equation accounted for 40.4％ of interindividual variation of Warfarin dose.Conclusions The ARMS-TaqMan real time PCR technique was established to analyse CYP2C9 1075 and VKORCl-1639 genotype.The technique can achieve the needs of clinical practice.Warfarin dosing by multivariable regression equation in patients could increase efficiency for Warfarin dose adjustment,and could give suggestion for the individualization and rationalization for Warfarin dose.

One biocontrol bacillus B3 was isolated from peanut rhizosphere, which showed a broad inhibition spectrum against various soil-borne plant phytopathogenic fungi. It was also found to be capable of utilizing beta-cypermethrin, chlorpyrifos and imidacloprid as the sole source of carbon for growth. The extent of degradation of them at initial concentration of 100 mg/L was 54.42%, 57.44% and 49.24% respectively within 7 days. Strain B3 was identified as Paenibacillus polymyxa based on morphological, physiological-biochemical properties, and 16S rRNA sequence analysis. This study indicated that strain B3 had potential application in biological control and soil bioremediation.

Objective To explore the influence of Lipo PGE1 on endothelin (ET-1) and interleukin (IL-6) in exhaled breath condensate(EBC) of patients with chronic obstructive pulmonary disease (COPD) and pulmonary hypertension(PH) and its clinical significance.Methods A total of 40 cases of patients with COPD and PH were randomly divided into thecontrol group and the treatment group,20 cases in each group.The control group was administered with the conventional treatments such as anti-infection,bronchodilator,antiasthma,expectorant and oxygen therapy;the treatment group was administered with Lipo PGE1 (10 μg/d,iv,for 10 days) besides the conventional treatments.ET-1 and IL-6 in EBC,pulmonary artery systolic pressure(PASP),arterial blood PaO2 and PaCO2,lung function FEV1/FVC,FEV1%pred in both groups were assayedbefore and after the treatment.Results There were no statistical difference between the two groups in the ET-1 and IL-6 in EBC,PASP,arterial blood PaO2 and PaCO2,FEV1/FVC,FEV1% pred before the treatment.After the treatment,the ET-1,IL-6 in EBC,PASP and arterial blood PaCO2 of the treatment group were lower than thoseof the control group (P<0.05);the arterial blood PaO2,FEV1/FVC,FEV1% pred of the treatment group were higher than those of the control group (P<0.05);The levels of ET-1 and IL-6 in EBC wereboth positivelycorrelated with PASP in the two groups.Conclusion Lipo PGE1 can reduce the levels of ET-1 and IL-6 in EBC of patients with COPD and PH.ET-1 and IL-6 may become a curative effect judgment index of COPD and PH,which has a certain clinical significance.

Objective To investigate the efficacy of acupuncture at mind-tranquilizing points plus conventional shoulder points in treating scapulohumeral periarthritis (periarthritis of shoulder) at the early stage of adhesion. Method Sixty patients were randomly allocated to two groups. The treatment group received acupuncture at mind-tranquilizing points plus conventional shoulder points and the control group, at conventional points. In both groups, treatment was given three times a week, for two weeks. The Hamilton Anxiety Scale (HAMA) score, the Hamilton Depression Scale (HAMD) score, the Japanese Orthopaedic Association (JOA) score and the Visual Analogue Scale (VAS) score were recorded before and after treatment. Result The VAS score,the HAMA score, the HAMD score and the JOA score improved in both groups after treatment compared with before (P<0.05). The VAS score and the HAMA score improved more in the treatment group than in the control group (P<0.05). There were no statistically significant post-treatment differences in the HAMD score and the JOA score between the treatment and control groups (P>0.05). Conclusion Mind-tranquilizing acupuncture plus conventional acupuncture is more effective than conventional acupuncture in treating scapulohumeral periarthritis at the early stage of adhesion.

Objective To analyze the effects of image quality and radiation dose of the patients with no heart rate control on different acquisition window settings.Methods A total of 75 patients underwent CTCA scan,with a median age of 55 years.All patients had no heart rate control.HR≤65 beats per minute acquisition phase setting was from 65%to 75%,65 beats per minute80 beats per minute acquisition phase setting was from 35%to 45%.Evaluated patients radiation dose and subjective image quality of coronary artery.Results 75 patients with ED was (2.44±1.21)mSv,group A was (1.54±0.41)mSv, group B was (3.57±0.53)mSv,group C was (1.64±0.27)mSv,group A vs group B(P=0.000),group A vs group C(P=0.854),group B vs group C(P=0.000).A total of 1 043 coronary artery segments of 75 patients were evaluated,with an average of 13.9 segments showed by every case,(3.66±0.51)scale.Group A was (3.75±0.44)scale,group B was (3.60±0.54)scale,group C was (3.38±0.67) scale,group A vs group B(P=0.000),group A vs group C(P=0.004),group B vs group C(P=0.032).Conclusion The prospective ECG gated sequence scan could obtain the image quality and avoid unnecessary radiation dose by adapting to the different heart rate.

A 60-years-old(85 kg,178 cm) coronary arterial disease patient having had acute myocardial infarction for several times received liver transplantation successfully.He had a previous episode of acute myocardial infarction associated with entire obstruction of right coronary 6 years ago,and was inserted a bracket then.One year ago the patient got chest pain again,and was diagnosed as inferior wall myocardial infarction.Then he received expectant treatment in internal medical department for several days.A 774HF75 PAC catheter(Edwards Lifescience,USA) was inserted into an internal jugular vein,and cardiac output,right ventricular end diastolic volume index,right ventricular ejection fraction,stroke volume index,system vascular resistance,pulmonary vascular resistance,left ventricular-stroke work index and right ventricular-stroke work index were calculated.During the operation,cardiovascular medications such as dopamine,norepinephrine,dobutamine were infused and adjusted by steps carefully.Electrolytes and acid-base balance were maintained normal.With these hemodynamic parameters,BGA and systemic management,the anesthesia was managed safely and successfully.

Objective:To explore the feasibility of guiding the volume management during orthotopic liver transplantation by right ventricular end-diastolic volume index(RVEDVI).Methods:Thirty-two patients who accepted OLT were studied during operation.A modified pulmonary artery catheter equipped with a fast response thermistor(774HF75)was used to determine RVEDVI,EF,CCI,CVP,MPAP,PAOP and SVI.The above-mentioned hemodynamic measures were taken in 9 phases:T0,before induction of anesthesia;T1,before operation;T2,pre-anhepatic phase;T3,30 minutes after PV occlusion;T4,10 minutes after graft reperfusion;T5,30 minutes after graft reperfusion;T6,60 minutes after graft reperfusion;T7,120 minutes after graft reperfusion;and T8,at the end of surgery.The linear regression analyses of SVI and CVP,SVI and PAOP,SVI and RVEDVI in each phase were calculated,and the best measure of representing the volume of the OLT patient was selected.Results:The variation of the RVEF value was(42.04?9.40)%.Linear regression analyses showed a significant correlation between RVEDVI and stroke volume index(SVI) in each phase(P

The dental abrasive testing machine applies a V-like swing arm to driving the wearing part to move along the slope back and forth, which is connected with an eccentric wheel fixed on the crankshaft of the gear motor through a connecting rod. Various abraders can be held on the wearing part with an adjustable wearing pressure between 10 and 500g loaded on the specimen. During wearing, both abrader and specimen are immersed in the wearing medium. Before and after the test, the specimen is weighed and its wearing surface is recorded by a profilometer, then the weight loss and volume loss can be calculated.