Objective:To investigate the inhibition of Eleo-Zedoary solution to human endometrial cancer cell line RL-952 in vitro and its possible mechanism.Methods:Using the MTT assay to display the sensitivity to the drug,the inhibition of Eleo-Zedoary parenteral solution to RL-952 cells in vitro was observed;and using light microscope and transmission electron microscope,the morphological alterations of the cells were observed.Results:The inhibition rate of Eleo-Zedoary parenteral solution to RL-952 indicated both dose-dependent and time-dependent anti-proliferative effect.Microscopically,the large vacuoles in the cytoplasm of the cells were found.Ultramicroscopically,many fat-like drops and obvious swelling of organelles as well as the mitochondrial cristaes in the cytoplasm of cells were observed.In addition the nucleus concentration and the chromatin fragmentation and coagulation,including the apoptotic bodies were observed.Conclusion:Eleo-Zedoary parenteral solution can inhibit the proliferation of human endometrial cancer cell line RL-952 by induing apoptosis and secondary necrosis.

Pharmacy is a professional subject with strong practicality and applicability,in which experimental teaching is an essential part of developping students' innovation and overall qualities. Based on the pharmaceutical experimental teaching status,some reform programs including experimental teaching measures,utilization of online teaching and optimization of evaluation manners were provided on the purpose of improving overall quality of the students and developing more comprehensive students.

This paper discussed the selection of SCI journals and questions associated with submitting manuscripts.The strategy for the submitting manuscripts.and the problems in the publication of manuscripts were also summarized in this article.These results would be valuable and guide for the researchers to submit their manuscripts,conform the writing skills of scientific papers,and improve the efficiency of SCI publications.

Objective To evaluate the academic level and the popularity of Chinese Journal of Laboratory Medicine.Method With Chinese Medical Citation Index(CMCI), we analyzed the amount and distribution of the originals in Chinese Journal of Laboratory Medicine cited by the journals indexed in CMCI.Result The percent of cited papers in all published articles was 55.68% during the period, the average number of cited times is 4.83, the top cited value was 108.206 articles (30.03% of total papers) were cited 5 or more, and the all cited times from them were 2428(73.33% of total cited times). 686 cited articles were written by 526 authors from different countries.The most frequently cited author wrote 12 papers, the authors with one paper cited were 430(81.75% of total authors).The cited authors are from the 31 provinces and other country; Beijing, Zhejiang and Shanghai are in the front of Laboratory Medicine research.Beijing was the top of rank (44.40% of total authors).292 institutes were cited and the most frequently cited institute was Beijing Hospital(10.48% of total cited times).There were 563 citing journals where the self-citing rate is 9.85%.Conclusion The Chinese Journal of Laboratory Medicine has published high quality articles. It is the one of the most important information resource for the laboratory medicine researchers and the most important medical journal.

Objective To describe the diagnosis and preoperation evaluation of pancreatic carcinoma with spiral CT three-phase contrastenhanced scan and post-processing.Methods Three-phase helical CT enhanced scan and post-processing images of 41 patients with pancreatic carcinoma were retrospectively analyzed,including the location,size,characteristic of tumor and peripancreatic blood vessels,the results were compared with that of surgery.Results Low degree of enhancement of pancreatic carcinoma in pancreatic phase was showed compared with parenchymal phase.There was significant difference in density between focus and normal pancreas,the lesions were showed clearly.In comparison with surgical results,the diagnostic accuracy of CT was 80.7% and the accurate rate in evaluating the metastasis was 75.8%.Conclusion Three-phase helical CT enhanced scan and postprocessing are of great value in diagnosis of pancreatic carcinoma and it is helpful in choosing the treatment program preoperatively and assessing the respectability of tumors.

Cardiomyopathies ; diagnosis ; Diagnostic Imaging ; Humans ; Ventricular Dysfunction, Left ; diagnosis

Objective The study is mainly on the effect and outcome of 131 I treatment for children and adolescents with differentiated thyroid cancer .Methods We compared the difference between rhTSH -aided ra-dioiodine treatment and L -thyroxin withdrawal .Our study collected 87 patients in a retrospective study .In order to analysis the effect,we divided into two groups.The experimental group has 46 patients,and control check has 41 patients.We compared the basic characteristics and mortality in different groups by χ2 -test,t-test and F-test based on data type .Results The thyroid stimulating hormone concentrations of experimental group patients have significant differences in the first ,the third and the sixth day after the injected of rhTSH ,the third day is the highest.The TSH concentration has significant difference between the two groups before 131I treatment(t=2.362, P=0.023).For the Tg(thyrobolulin)negative patients,Tg concentration in control check is higher than the third day after injected of rhTSH in the experiment group (1.5 ±1.2 vs.0.7 ±1.4,P=0.034).Whole body image has been done by scintigraphic after 3~8 months of 131I treatment.Thirty four(84%)patients have no radioiodine up-take in experiment group,and 40(87%)patients have no radioiodine uptake in control check ,there is no differ-ence between the two groups (χ2 =0.277,P=0.599).There is no difference between the two groups on the rea-son for retreatment(P=0.875).Conclusion The outcome of rhTSH -aided radioiodine treatment is similar to L-thyroxin withdrawal in short -term recurrences ,and rhTSH-aided radioiodine treatment with 131 I therapy for children and adolescents with DTC is a better method .

ObjectiveTo investigate the expression and significance of CDX2,Ki-67 in adenoma and colorectal adenocarcinoma,and the correlation between CDX2 and Ki-67.MethodsThe expression of CDX2 and Ki-67 was evaluated in the colorectal adenocarcinoma,adenoma and normal colorectal mucosa as control subjects by immunohistochemistry.ResultsThe magnitude of CDX2 were negatively correlated with the level of dysplasia in adenoma(rs=-0.38,P =0.02),and negatively correlated with differentiation,lymph node metastasis,diversion and Dukes stage of cancer in colorectal adenocarcinoma(rs=-0.49,rs=-0.37,rs =- 0.38,rs =-0.37,P =0.01).The expression and relativity of Ki-67 in the samples were contrary to the CDX2.The levels of CDX2 and Ki-67 expression had significant difference between the adenoma and adenocarcinoma,and showed negatively correlation in this two type tissues (rs=-0.69,P =0.00; rs =-0.40,P =0.00).ConclusionsCDX2 may be used as an effective marker to evaluate malignancy degree and prognosis of large bowel neoplasm patient.The levels of Ki-67 expression in colorectal adenocarcinoma are correlated with malignancy degree and clinical stage.The expression of CDX2 is negatively correlated with Ki-67.Combined detection of CDX2 and Ki-67 may be helpful for the judgment of colorectal tumor biological characters.

Coronary Angiography ; Coronary Disease ; diagnosis ; diagnostic imaging ; Diagnostic Imaging ; methods ; Humans

Vascular eognitive impairment (VCI) is a large class of syndromes from mild cognitive impairment to dementia, which are caused by the cerebrovascular risk factors (eg, hypertension, diabetes and hyperlipemia), obvious (eg, cerebral infarction and intracerebral hemorrhage) or unobvious cerebrovascular diseases (leukoaraiosis and chronic cerebral ischemia). Accurate early diagnosis provides the best treatment opportunity and slows down the progress of the disease. In recent years, the research on VCI has made a great progress. This article reviews VCI from the aspects of the etiology, clinical manifestation, neuropsychology, imaging, neuroelectrophysiology, biomarkers, and diagnostic criteria.