Objective:To investigate the inhibition of Eleo-Zedoary solution to human endometrial cancer cell line RL-952 in vitro and its possible mechanism.Methods:Using the MTT assay to display the sensitivity to the drug,the inhibition of Eleo-Zedoary parenteral solution to RL-952 cells in vitro was observed;and using light microscope and transmission electron microscope,the morphological alterations of the cells were observed.Results:The inhibition rate of Eleo-Zedoary parenteral solution to RL-952 indicated both dose-dependent and time-dependent anti-proliferative effect.Microscopically,the large vacuoles in the cytoplasm of the cells were found.Ultramicroscopically,many fat-like drops and obvious swelling of organelles as well as the mitochondrial cristaes in the cytoplasm of cells were observed.In addition the nucleus concentration and the chromatin fragmentation and coagulation,including the apoptotic bodies were observed.Conclusion:Eleo-Zedoary parenteral solution can inhibit the proliferation of human endometrial cancer cell line RL-952 by induing apoptosis and secondary necrosis.

Pharmacy is a professional subject with strong practicality and applicability,in which experimental teaching is an essential part of developping students' innovation and overall qualities. Based on the pharmaceutical experimental teaching status,some reform programs including experimental teaching measures,utilization of online teaching and optimization of evaluation manners were provided on the purpose of improving overall quality of the students and developing more comprehensive students.

Coronary Angiography ; Coronary Disease ; diagnosis ; diagnostic imaging ; Diagnostic Imaging ; methods ; Humans

Cardiomyopathies ; diagnosis ; Diagnostic Imaging ; Humans ; Ventricular Dysfunction, Left ; diagnosis

Objective The purpose of this study is to analyse the expression of tumor necrosis factor (TNF)-related apoptosis inducing ligand(TRAIL)and its receptors in muscle tissue from patients with idiopathic inflammatory myopathies(ⅡM)and investigate the possible role of TRAIL system in the Dathogenesis of IIM.Methods TRAIL and its receptors DR4,DR5,DCRl and DCR2 were detected in the musck biopsy tissue from 36 patients with ⅡM(13 polymyositis,23 dermatomyositis)and 9 heMthy controls bv using immunohistochemistry. Results The expression of TRAIL and its receptors was f10und in muscle tissue samples from the IIM patients and healthy controls.The expression of TRAIL,DR4 and DCR2 in the muscle tissue from the IIM patients was significantly higher than those from healthy controls(all P values< 0.05).The expression of TRAIL,DCRl and DR4 was also detected in the infiltrating lymphocytes in endomysium and in the interstitial tissue around blood vessels.Conclusion The diversity in expression of TRAIL and its receptors between patients with IIM and healthy controls suggests the hypothesis of a crucial role of TRAIL in the pathogenesis and the pathology of IIM.

Objective To describe the diagnosis and preoperation evaluation of pancreatic carcinoma with spiral CT three-phase contrastenhanced scan and post-processing.Methods Three-phase helical CT enhanced scan and post-processing images of 41 patients with pancreatic carcinoma were retrospectively analyzed,including the location,size,characteristic of tumor and peripancreatic blood vessels,the results were compared with that of surgery.Results Low degree of enhancement of pancreatic carcinoma in pancreatic phase was showed compared with parenchymal phase.There was significant difference in density between focus and normal pancreas,the lesions were showed clearly.In comparison with surgical results,the diagnostic accuracy of CT was 80.7% and the accurate rate in evaluating the metastasis was 75.8%.Conclusion Three-phase helical CT enhanced scan and postprocessing are of great value in diagnosis of pancreatic carcinoma and it is helpful in choosing the treatment program preoperatively and assessing the respectability of tumors.

?AIM: To explore the application of 25G vitreous cavity perfusion in phacoemulsification for cataract after vitrectomy.?METHODS:Forty-eight patients (50 eyes) with cataract after pars planavitrectomy in our hospital from September 2013 to September 2014 were selected to receive phacoemulsification and implantation of artificial lens with 25G vitreous cavity perfusion. Best corrected visual acuity ( BCVA ) , intraocular pressure ( IOP ) , postoperative discomfort, healing of scleral puncture and postoperative complications were observed.?RESULTS: All patients underwent surgery successfully. Compared to before operation, there was less patients with BCVA ≤0. 1, more patients with BCVA 0. 3-0. 5,>0. 5,>0. 3 (P<0. 05). The visual acuity of 1 eye decreased because of vitreous hemorrhage caused by original fundus lesion recurrence. The mean IOP before operation and at 1, 7d, 6mo after operation were 14. 67±0. 62mmHg, 14.75±0.56mmHg, 14.86±0.58mmHg, 14.88±0.59mmHg, respectively. There were no statistically significant differences of IOP between that before operation and those at 1, 7d, 6mo after operation (P>0. 05). No obvious eye discomfort and severe complications after operation were observed.? CONCLUSION: The effect of 25G vitreous cavity perfusion in phacoemulsification for cataract after vitrectomy is good and it can maintain IOP, keep anterior chamber stable and decrease the complications risk.

Objective: To investigate the relationship between the serum ferritin concentration and insulin resistance in patients with impaired glucose tolerance(IGT).Methods: The serum ferritin concentration and insulin levels were measured in 40 IGT patients(20 with normal body weight and 20 with obesity),and 40 healthy controls.Blood glucose levels,HbA1C,blood adipose and urine protein concentration were obtained simultaneously.Results: In the IGT patients,the serum ferritin concentration was correlated with Hba1c but not with any other indexes,and it was significantly higher than in the normal controls.Significant differences were observed between the two groups in all the indexes but the fasting insulin level.Conclusion: The serum ferritin concentration increases in IGT patients,which implicates some association of ferritin with glucose metabolism and insulin resistance.

Chemical Weapons Convention (CWC) and Biological Weapons Convention (BWC) are the two important international conventions in the disarmament field ,aimed to eliminate chemical and biological weapons by prohibiting the development, production, acquisition, stockpiling and use of these weapons .Recently, research and development of sci-ence and technology continue to advance rapidly .A key feature is convergence of science disciplines .This has become a new force to speed up development of science and economy .In particular , the convergence of chemistry and biology , together with the rapid advances in life sciences , has been evident in its remarkable contribution to the developments of pharmaceutics, health care, green chemistry and environmental protection .On the other hand, the convergence of chemistry and biology also has significant implications for the implementation of CWC and BWC .In this article, we summarize the new trends in the convergence of chemistry and biology and its impact on the conventions .

Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.