Multiple myeloma (MM) is a malignant plasma cell disease, accounting for about 1% of all malignant tumors, is still uncurable, minimal residual disease (MRD) is the reason, therefore, removal of minimal residual disease maybe possible to cure the disease. In a variety of treatment methods, immune gene therapy for multiple myeloma is a hot research topic in recent years, has achieved good results in vitro and in vivo experiments, in this review immune genes therapy in multiple myeloma research would be discussed.

Objective To investigate the effects of solution focused approach on the hope level of patients with epilepsy. Methods A total of 83 patients with epilepsy were randomly divided into the observation group and the control group, 42 cases in the observation group, 41 cases in the control group. The control group was given traditional nursing care, the observation group was given nursing intervention based on solution focused approach. Two groups were surveyed with Herth Hope Index (HHI), Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) before and after the intervention. Results Before the intervention, HHI of the observation group and the control group scored 24.95 ± 1.92 and 24.56 ± 3.23 respectively. After intervention the scores increased to 28.85 ± 1.81 and 25.31 ± 3.35. After intervention, HHI and each dimension of the observation group scored higher than those before intervention and the control group, the difference was statistically significant (t=-4.75, 5.84, P < 0.01). Before the intervention, SAS scores in the observation group and the control group were 56.41 ± 5.97 and 56.28 ± 6.14, SDS scores were 53.72 ± 8.41 and 53.47 ± 8.26, there was no statistically significant difference between the 2 groups (t=0.09, 0.13, P > 0.05). After intervention SAS scores the observation group and the control group were 41.65 ± 6.22 and 49.72 ± 5.86, SDS scores were 44.57 ± 5.83 and 49.73 ± 6.24, there was significant difference between 2 groups (t=5.67, 3.20, P < 0.01). Intra group comparison of the observation showed that there was significant difference in SAS and SDS scores before and after the intervention (t=10.27, 5.36, P < 0.01). Intra group comparison of the control group also showed statistical difference in SAS and SDS scores (t=4.65, 2.17, P<0.05 or 0.01). Conclusions The solution focused approach can improve the psychological status of patients with epilepsy, improve the level of hope, promote the rehabilitation of patients.

BACKGROUND:Neuronal calcium sensor-1 protein has a variety of different neuronal functions and has a high distribution in different areas of the brain. A single residue R102Q mutation in human neuronal calcium sensor-1 protein is demonstrated to be associated with autism disease. The experiment studies have reported that this R102Q mutant has essential conformation changes in local area of the neuronal calcium sensor-1. OBJECTIVE: To wel understand the specific reasons of the R102Q mutation of the neuronal calcium sensor-1 to the conformational dynamic changes. METHODS:Six independent extensive al-atom molecule dynamic simulations during 0-450 ns were conducted. RESULTS AND CONCLUSION: We have found that (1) there is no obvious recombination during the simulations between wild type and mutant type, but R102Q mutant alters the helix and makes the structure of the protein more stable; (2) R102Q mutation alters the salt bridges, reduces the flexibility of L2, and makes L3 extend in hydrophobic crevice. These results reveal that the helix plays an important role in the structural stability, and salt bridge is the important reason for the dynamic changes of neuronal calcium sensor-1 protein. This study may provide a structural insight into the function of protein deficiency associated with R102Q mutant.

Objective To investigate the value of methylprednisolone pulse therapy on the acute treatment of multiple sclerosis.Methods 46 cases of the acute phase of patients with multiple sclerosis treatment in the hospital's department of neurology were chosen,who were divided in accordance with the principle of randomized controlled study group and the control group,each group of 23 patients.The study group was given methylprednisolone in the treatment and control group were given dexamethasone treatment.The clinical efficacy and complications of the treatment of patients were compared.Results The study group markedly in 14 cases,effective 54 cases,the total effective rate was 82.6％ ;the control group,10 casesmarkedly effective in 4 cases,9 cases ineffective,the total effective rate was 60.9％ ; compared the two sets of data decline before treatment,there was a statistically significant difference (x2 =5.236,P ＜ 0.05).After the treatment,the two groups of patients with EDSS scores compared with EDSS scores of the study group was significantly lower than that of the control group,the difference was statistically significant(t =3.135,P ＜ 0.05).Conclusion In acute phase of patients with multiple sclerosis,methytprednisolone pulse therapy can reduce the incidence of complications,and reduce the patient's nervous system damage,which can improve the quality of life in patients.

[Objective]To observe the effect of Chinese herbs for activating blood stasis on the shape and function of the endothelial cells in rat aorta.[Method]Rat models of PCOS have been established according to Poresky,randomized into animal model and Chinese medicine group(TCM group),normal rats were control group.Measure the serum level of FSH,LH,T,INS and plasma ET PAI-1,and observe the endothelial cell morph.[Results] In the model group,the endothelia cells of the aorta were contracted,with discontinuous basement membrane,inflated chondriosome,the middle layer smooth muscles tending to move inside and out of the normal shape of intraendothelial junction.In the TCM group,the endothelial cells appeared flat,arranging along the long axis of the blood vessels,normal endothelial cell integrity with scanty cytoplasm and the microscopic appearance shows highly developed golgiosome rough endoplasmic reticulum and smooth endoplasmic reticulum.The model group had a more increased serum level of PAI-I and ET than those of the control group,and showed the significant difference(P0.05).The TCM group had more decreased level of ET than those of the normal group(P

0.05). Mutation frequencies induced by AME at different dosage in the presence of 15?g/ml RA Ⅱ were lower than that of AME alone (P

BACKGROUND:Research front and hotspots of neuronal calcium sensor-1 are always the focus for the researchers in this field.
OBJECTIVE:To probe the research front and hotspots of neuronal calcium sensor-1 with the methods of quantitative analysis.
METHODS:The methods of co-cited articles analysis and word frequency analysis were used in the article. The objects were 363 articles from Web of Science by US Institute for Scientific Information (ISI) about the neuronal calcium sensor-1 from 1982 to 2014. The network of co-cited articles and keywords was showed in visualization mapping by using CiteSpace III in which the burst nodes represented the high impact hot papers and the most frequently used keywords, and revealed the research frontier and the hot spots of neuronal calcium sensor-1. RESULTS AND CONCLUSION:The physiological functions of neuronal calcium sensor-1 are the research
frontier and the hot spots. The transformational point in time spot of the hotspots is during 1994 to 1996, 2000, 2008, 2012;and the different research focus showed in each stage:the structure and characterization of the protein during 1992-2000 and the protein function and the role during 2004-2012 are the research hotspots, while during 2008-2014 the hotspots place extra emphasis on the higher function (e.g. memory) and several diseases(such as schizophrenia, cancer, autism, depression, senile dementia, neuron damage, etc). The determination of the research frontier domains and hot spots of neuronal calcium sensor-1 wil indicate the goal and direction for the further studies.

BACKGROUND: Physiological functions, structural fold and unfolding of neuronal calcium sensor-1 (NCS-1) have been explored in a series of experiments, and then the possible mechanism models and key factors for remaining the structural stability are raised. But many functional models cannot be verified due to the limitations of resolution of the time and space and complex protein structure. The experimental phenomena and hypothesis or models may be tested at the atom levels by molecular dynamics, and the new structure may be predicted to provide basis for model establishment and functional mechanisms. OBJECTIVE: To overview the research process of physiological functions and mechanisms of NCS-1 using the experimental method and molecular dynamics simulations, thereby providing basis for future research.METHODS: PubMed database was retrieved for the literatures addressing NCS-1 using the English subject term Neuronal Calcium Sensor-1 or Neuronal Calcium Sensor1 or Neuronal Calcium Sensor 1 or NCS-1. Finally, 72 articles were included in result analysis based on the inclusion and exclusion criteria. RESULTS AND CONCLUSION: The theoretical models of NCS-1 in secretion regulation, dopamine D2 receptor regulation, adenosine A2A receptor regulation in hepatocytes and Ca2+ regulation in myocardial cytoplasm and nuclei with different stimuli are put forward. The key factors to remaining structural stability are analyzed and summarized by modular dynamics simulation in view of structure. It is recommended to combine these two methods in order to deeply understand the protein functional mechanisms, thereby pushing the in-depth study.

OBJECTIVE To provide more evidence for hospital infection control.METHODS To(analyze) the clinical records of the hospital-acquired pneumonia(HAP) of senile patients in department of(neurology) during Jan 2004 to Dec 2005 retrospectively.RESULTS There were 109 person-times(145 cases) senile(patients) with hospital(infection) during the two years.From them 77 cases were hospital-acquired infection,32 cases were ventilator-(associated) pneumonia(VAP),the infection rate was 22.1%.CONCLUSIONS The infection of HAP in department of neurology may induce by several factors.The key point to control HAP is prospective monitoring hospital(infection) in time,and taking effective measures to prevent the hospital infection on the basis of treatment the(underlying) disease.

OBJECTIVE:To evaluate the effects of minidose aspirin on blood uric acid,creatinine and urea nitrogen in hyperuricemia model mice.METHODS:75 mice were randomly assigned to receive 0.5% CMC-Na(normal control group and hyperuricemia control group)or aspirin(12.5,25,50 mg?kg-1?d-1)q.d for 1 week.Then all the mice except the normal control group were intraperitoneally administered with uric acid to establish hyperuricemia model.1 h later,the blood sample was collected for determination of uric acid,creatinine and urea nitrogen.RESULTS:As compared with hyperuricemia control group,mini-dose aspirin had no apparent effect on creatinine and urea nitrogen,but remarkably increased the level of blood uric acid(P