Objective To observe the clinical effects of treating LIDP with needle-knife therapy plus balance.acupuncture therapy.Methods 120 LIDT patients were randomly recruited into a treatment group and a control group.with 60 patients in each group.The treatment group was treated with needle-knife therapy plus balance-acupuncture therapy andthe control group was treated with needle-knife therapy exclusively.The therapeutic effects were evaluated after thetreatment.Resuits There was significant difference between the two groups in terms of recovery rateandthe course of treatment. Conclusion Needle-knife therapy plus balance-acupuncture therapy was obvious better than exclusive needle-knife therapy in treating LIDT in terms of recovery rate and the course of treatment.

[Objective]To approach the methods of difficult laparoscopic cholecystectomy.[Methods ]The clinical data of 116 patients with Mirizzi syndrome,severe adherence of abdominal cavity and Calot'triangle,liver cirrhosis underwent difficult laparoscopic cholecystectomy since 2004 were retrospectively analyzed.[Results]Except 3 patients treated by abdominal operation,other patients were treated by laparoscopic cholecystectomy successfully.[Conclusion]The rates of open operation midway and complications are not increased in difficult laparoscopic cholecystectomy as long as dealing with them properly.

Objective To compare the contents of guanosine and adenine in peels of three strains of Trichosanthes kirilowii Maxim.;To provide references for germplasm resources evaluation and breeding of the Chinese medicinal materials. Methods HPLC method was used to determine the contents of guanosine and adenine with Waters AtlantisT3-C18 column (4.6 mm×150.0 mm, 5 μm). The mobile phase was methanol and water, and the detection wavelength was 254 nm. Results The contents of guanosinein in peels of three strains of Trichosanthes kirilowii Maxim. were 0.159 4, 0.159 6, 0.134 1 mg/g, respectively;the contents of adenine were 0.097 1, 0.127 9, 0.093 4 mg/g, respectively. Conclusion There were significient differences in the contents of adenine among three strains of Trichosanthes kirilowii Maxim.. Strain Ⅱ is with higher level of guanosine and adenine, which implies that the breeding of the Chinese medicinal materals is one way to improve the quality of Trichosanthis Exocarpium.

Objective　This was a follow-up study of suicidal patients to assess the influence over time of different risk factors.Method　A cohort of 73 patients admitted to a psychiatric department after a suicide attempt was followed-up for 5 years.Results　In total,61.6% reattemped,15.1% of which had committed suicide during the follow-up.The rist factors for suicide included history of family psychiatric or suicide,and a poor social support,et al.Conclusion　The results suggest a high repetition rate of suicide attempters in the psychotic patients who had previous suicidal behaviour and them positive family history.The preventive strategy to suicide in patients,such as set up good social network and should pay mroe attention to psyciologic rehabilitation period after discharge.

Objective To observe the changes and the mechanism of bcl-6 expression in K562 cells inducing differentiation along different Myelocyte-lineage cells,and to investigate the relationship between expression of bcl-6 and directional differentiation of K562 cells.Methods Models of K562 cells inducing differentiation along megakaryocyte-lineage,erythrocyte-lineage and macrophagocyte- lineage were established with inducers TPA(tetradecanoylphorbol 13-acetate),Hu(hydrozyurea)and HMBA(hexamethylene bisacet- amide)respectively,Western blot assay was applied to detect the expression of bcl-6 in K562 cells before and after the induction.Mean- while,PCR,cloning and direct DNA sequencing were used to identify mutations in the 5'regulatory region of bcl-6 in K.562 cells induced with TPA.Results Up-regulation of bcl-6 expression was found only in K562 cells inducing differentiation along megakaryocyte-line- age,and mutation of noncoding regulatory region of bcl-6 gene was not found.Conclusions bcl - 6 may be the key gene to regulate K.562 cells directional differentiation along different myelocyte-lineage ceils,the up-regulation of bcl-6 expression could play an important role in K562 cells inducing differentiation to megakaryocydte-lineage,and the change of bcl-6 expression might be not related with the mutation of the gene's 5'regulatory region.

Hydrolysate of Area subcrenata (HA) was found to be effective in lowering the elevated SGPT level induced by carbon tetrachloride, thioacetamide (TAA) and pred-nisolone. In addition, HA was shown to shorten sodium pentobarbital sleeping time in both normal and carbon tetrachloride intoxicated mice. The results indicated that HA possessesa significantl protective action on liver injuries in mice.

Objective To observe the effect of lattice silver wound repair dressings on large-area abrased wounds . Methods A total of 60 patients with large-area skin abrasions were randomly divided into the observation group and the control group with 30 cases in each group. Both groups were treated with the same antibacterial treatment and nutritional support treatment. The control group received conventional general routine dressings and the observation group had the silver wound repair dressings every day. The two groups were compared in terms of the wound pain, wound infection rate, time for wound healing and scars of patients. Results The wound healing time in the observation group was significantly shortened (P<0.01). The scaring of the observation group was better than that in the control group (P<0.01). The pain degree and the exudation rate were both significantly lower in the observation group than those of the control group (P<0.01), and wound infection rate was lower than that of control group. Conclusions The lattice silver wound repair dressing can effectively promote the wound healing, shorten hospitalization duration, promote the patient recovery soon. It is simple and worthy of clinical popularization.

Abstract: There were more and more patients suffered from perianal abscess. The patient's condition will become even worse if the patients cannot be treated properly. Selecting proper operation method according to special condition is the key point of increasing curative effect, decreasing suffering and relapse rate and promoting quality of life of patients. The authors summarized operation methods, including abscess incision drainage, radical incision and thread-drawing, and staging incision and thread-drawing, etc. in treating perianal abscess in clinic.

In recent decades, the development of the neurosurgery has changed from the traditional anatomical model to the modern anatomical-functional model. The nerve functions are maximally protected while lesions are removed as far as possible. Neurophysiological monitoring especially somatosensory evoked potentials (SEPs) and motor evoked potentials (MEPs) can directly reflect the integrity of the sensory and motor nerve conduction pathways of the nervous system. At present, it has been widely used in the neurosurgery, spinal surgery, vascular surgery and other surgical fields. In recent years, more and more clinical surgeries involved spinal surgery, intraoperative neurophysiological monitoring could timely find any reversible spinal cord damage such as mechanical stretch, ischemia, and anesthetic drugs, which not only improve the quality of surgery, reduce iatrogenic spinal cord injury, but also greatly improve the prognosis of patients and reduce postoperative neurological dysfunction and complications. In this paper, the research progress of neural electrophysiological monitoring techniques in spinal cord surgery is reviewed.

Objective To investigate the association of rs10004195 single nucleotide polymorphisms in Toll like re-ceptor 10(TLR 10) gene with helicobacter pylori infection and associated diseases. Methods A total of 652 pa-tients who has been examined by gastroscopy were obtained, and then peripheral blood samples were collected from all the patients. Immune colloidal gold method was used to test the serological Hp antibody of all the patients. The TLR10 gene rs10004195 polymorphisms were examined by direct DNA sequencing of the PCR products. Results The frequencies of AA,TT and AT genotype on TLR10 rs10004195 were 30. 98%, 20. 71%, 48. 31%;there was significant difference between Hp antibody positive group and Hp antibody negative group in the TT frequencies of TLR10 rs10004195 ( P<0. 05 ) . No significant difference between controls and Hp associated diseases groups in Hp antibody positive group or in Hp antibody negative group were observed. Conclusion There was correlation be-tween the TLR10 rs10004195 loci genotype and the risk of Hp infection, but no correlation with Hp associated dis-eases.