Objective To explore the effect on the differentiatiation of bone marrow mesenchymal stem cells(BMSCs) induced by Ganglioside(GM1) into neural cells.Methods Rat BMSCs were isolated on the basis of its ability to adhere to the culture plate,passaged three times,and finally added GM1 to induce its differentiation.The morphologic changes of BMSCs were observed under phase-contrast microscopy,then neuron specific enolase(NSE) and glial fibrillary acidic protein(GFAP) expression were determined by immunocytochemistry way.Never growth factor(NGF) mRNA and brain-derived neurotrophic factor(BDNF) mRNA expression were detected with RT-PCR and then compared with those of FBS control group and blank control group.Results In GM1 induction group,BMSCs appeared round-shaped,with some dendrite and axon interlaced.(29.47%?3.26)% BMSCs showed positive staining to NSE,while(2.32?0.18)% were positive to GFAP.(6.97?0.56)% and(10.6?0.75)% BMSCs were positive to NSE in FBS control group and blank control group respectively,while(1.41?0.35)% and(1.21?0.35)% BMSCs were positive to GFAP in FBS group and blank control group respectively.NGF mRNA and BDNF mRNA levels in GM1 induction group were significant higher than those in the FBS group(all P

Objective To investigate the change and its clinical significance of plasma insulin and leptin level in the patients with cerebral infarction.Methods The plasma leptin, insulin, glucose levels and insulin sensitivity index(ISI) of 31 patients with atherothrombotic cerebral infarction (ACI), 30 patients with lacunar infarction (LI) and 21 cases of healthy controls were determined. Results Compared with those in the controls, there were elevation of plasma insulin level (6.17 ? 4.33 ?IU/ml, P

Objective To investigate the relationship between plasma resistin level and insulin resistance in the patients with cerebrovascular disease.Methods Fasting plasma resistin and insulin (INS) protein levels were determined by ELISA method in 50 patients with atherothrombotic cerebral infarction (ACI), 36 patients with lacunar infarction (LI), 36 patients with intracerebral hemorrhage (ICH) and 46 healthy control subjects. Blood glucose, blood lipid, blood pressure, height and weight were measured. Body mass index (BMI) and quantitative insulin sensitivity check index (QUICKI) were caculated as well.Results Compared with the control subjects, there were significantly higher fasting plasma insulin protein level and lower QUICKI in the ACI and ICH patients ( P

Objective To establish a rapid determination of sodium valprate in plasma by capillary zone electrophoresis.Method Plasma was acidized by 1 mol/L HCl, VPA was extracted into organic phase (n-Hexane), then re-extracted into aqueous phase (0.05 mmol/L NaOH solution including 15% Ethanol). Capillary clone was 75 ?m(id)?37 cm. Electrolyte consisted of 15 mmol/L sodium salicylate, 0.5 mmol/L CTAB and 15% Ethanol (pH 5.7).Separating voltage was 20 kV, detection wave was 214 nm, temperature was 20℃,injection time was 5 s by press in negative.Result The linear ranger of concentration for standard drug was between 25~200 ?g/ml (r=0.999),the limit of detection was 0.35 ?g/ml, the average recovery of VPA was 87.4%,the average inter-day and intar-day CV were less than 4% and 6%. Conclusion This method is simple, rapid, reliable and correct, for determination of VPA in plasma.

0.05).Conclusion The gene polymorphism of MMP-9/C1562T may be unrelated with IS.

Objective To investigate influence of Ang-(1-7) on the inducible nitric oxide synthase (iNOS) activity and gene expression following focal cerebral ischemia/reperfusion in rats. Methods Cerebral ischemia/reperfusion injury was induced by intraluminal thread occlusion of middle cerebral artery in the adult male Sprague-Dawley (SD) rats. Ang-(1-7) or artificial cerebrospinal fluid (aCSF) was continuous administrated by implanted Alzet osmotic minipumps into lateral cerebral ventricle after reperfusion. Experimental animals were divided into sham-operated group ( sham operation + aCSF), aCSF treatment group(MCAO+aCSF)and ang-(1-7)treatment groups(MCAO+Ang-(1-7))at low(1 pmol·0.5 μl-1·h-1),medium (100 pmol·0.5 μl-1·h-1)or hith(10 nmol·0.5 μl-1·h-1)dose levels.The activity of iNOS in ischemic tissues were measured by iNOS detection kits. Reverse transcription( RT)-PCR was used to determine messenger RNA (mRNA) of the iNOS in ischemic tissues. Results The cerebral ischemic lesion resulted in a significant increase of iNOS expression compared with sham operation group. The high-dose Ang-(1-7) markedly enhanced (iNOS) activity ( 160. 83 vs 116. 75 U/mg, F = 19. 22,P＜0.01; 151.87 vs 113.07 U/mg, F=63.52,P＜0. 01) and gene expression(0.43 vs 0.38, F=21.83,P ＜ 0. 01; 0. 40 vs 0. 35, F = 19.49, P ＜ 0. 01 ) compared with aCSF treatment group at 24 hours and 48hours after reperfusion, whereas medium and low-dose Ang-( 1-7 ) didn't stimulate iNOS activation.Conclusions The obtained results suggest that high-dose Ang-(1-7) upregulate iNOS expression following ischemic stroke.Moreover,overdose Ang-(1-7)(10 nmol·0.5 μl-1·h-1)may have Ang Ⅱ-like effects in iNOS expression increase.

Objective To investigate the expression of endothelin converting enzyme (ECE) mRNA in patients with acute cerebral infarction (ACI) and its clinical significance.Methods Blood samples from 40 patients with ACI (patient group) within 72 hours after the onset of ACI and 28 gender and age-matched healthy subjects (control group) were collected on admission. Plasma concentrations of endothelin-1 (ET-1) as well as the serum levels of cholesterol, triglyceride, low density lipoprotein,high density lipoprotein, apoA1, apoB, lipoprotein a and fasting plasma glucose in each sample were measured and analyzed. Additionally, semi-quantitative RT-PCR was performed to check the ECE mRNA level in the blood cells. European stroke scale (ESS) was used to evaluate ACI patients' neurological deficit on admission.Results (1) ECE mRNA could be detected in every blood sample from either patient group or control group. However, the ECE mRNA level increased significantly in the patient group compared with that in the control group (0.31?0.092 versus 0.25?0.10, t=2.46, P=0.016). (2) The plasma ET-1 concentration in patient group was also significantly higher than that of control subjects (183.27?56.63pg/ml versus 156.47?34.24 pg/ml, t=2.23, P=0.029). (3) Plasma ET-1 concentration was negatively correlated with ECE mRNA level in the control group (r=-0.452, P=0.021). However, the result in the patient group was not the same as the control group. (4) The ET-1 concentration and ECE mRNA level in the patients had histories of hypertension, diabetes mellitus and stroke were not significantly different from those in the patients without these histories. (5) No significant correlation existed between plasma ET-1 concentration and ECE mRNA level and age of the patient, ESS score, fasting plasma glucose and serum lipid. Conclusions ECE mRNA level is significantly increased in the early stage of ACI, which may be associated with the acute-phase reaction of cerebral infarction and may have deleterious effects on the development of neuronal injury. Our results suggest the protective reflection in the endothelin system of normal human body may be disturbed by the onset of ACI. The relationship between ECE mRNA level and neurological deficit degree, stroke risk factors is worthy for further study.

Objective To investigate the influence of Levetiracetam(LEV) and Topiramate(TPM) on P-glycoprotein(P-gp) expression at brain in the epileptic rats.Methods The epileptic rat models were made with 1.5 ?g(3 ?l) Kainic acid stereotaxic injected into male SD rats' hippocampi(epilepsy group) and the control group rats were injected with 3 ?l normal saline(NS).The rats in epilepsy group(18 rats) and control group(15 rats) were randomized into TPM,LEV and NS subgroups(6 or 5 rats for each subgroup) to receive once-daily orally feeding of TPM(50 mg/kg),LEV(40 mg/kg) and NS(same amount NS) for 30 d.Immunohistochemistry(EnVision) was used to measure the level of P-gp exprssion in the rats brain.The level of P-gp expression was semi-quantitatively analyzed by mean integrated blue(MIB) value in Leica Qwin imaging analysis & measuring system.Results The expression levels(MIB value) of P-gp in hippocampus and temporal lobe in epilepsy subgroups were significantly higher than those in the corresponding control subgroups(P

Objective To investigate the association of the gene polymorphism of plasminogen activator inhibitor 1 (PAI 1) with the cerebral infarction and recurrent cerebral infarction (RCI). Methods The plasma PAI 1 activity, by means of chromgenic substrate assay, and the sequence polymorphisms of 4G/5G in promotor region and (CA)n dinucleotide repeats in the 4th intron of PAI 1 gene, by amplified fragment length polymorphism assay, were measured in 50 patients with first ever cerebral infarction (FCI), 45 patients with RCI and 60 healthy controls.Results The plasma PAI 1 activities in both FCI patients (1.13?1 1 AU/ml) and RCI (1.13?0.150 AU/ml) were remarkably higher than that in the controls (0.7?0.25 AU/ml) (both P

Objective To investigate the role of mutation of insulin receptor(IR) gene on the development of ischemic cerebral vascular disease.Methods The base variations at exon 17 and 20 of IR gene, by means of PCR SSCP were determined in the 68 cases of atherothrombotic cerebral infarction (ACI), 81 cases of lacunar infarction (LI) and 62 healthy controls.Results There were two alleles of T and C at exon 17 of IR gene. The prevalence of mutant of T allele in ACI patients was more common than that in the controls. The blood pressure and the parameters of blood sugar,lipid metabolism in the patients with mutant were higher than those in the controls with wild type gene. The correlative analysis showed the polymorphism of IR gene was not related statistically to the blood pressure. No base variation at exon 20 was found in the study.Conclusion By promoting the development of atherosclerosis,the mutation at exon 17 of IR gene may participate in the occurrence of ischemic stroke.