Group B Streptococcus ( GBS) is a conditional pathogenic bacteria related to late-term abortion, premature delivery, premature rupture of membranes, perinatal infection, neonatal sepsis and other diseases.Prevention and treatment guidelines by the US Centers for Disease Control and Prevention ( CDC) suggest that all the pregnant women at 35-37 gestational weeks should screen for GBS .The detection methods for GBS include microbiology , immunology, molecular biology, etc.The appropriate method should be chosen depending on circumstances .Penicillin is recommended for the preventive treatment of GBS , and the treatment for the insensitive should base on the antibiotic susceptibility results .No vaccine against GBS is currently available for clinical use .

Objective:To prepare DHAQ-GM-CSF immunoliposomes and observe the cytotoxicity of HL60 cells treated with the immunoliposomes for providing the preclinical data for AML therapy. Methods:DHAQ liposomes were prepared by reverse-phase evaporation(REV),and GM-CSF was coupled to the DHAQ liposomes.The cytotoxicity of HL60 cells treated with the immunoliposomes was measured by tetrazolium microculture(MTT) celluar cytotoxicity test.The data of cellar untrastructure and apoptotic rate were collected by transmission electron microscopy,and flow cytometric analysis. Results:DHAQ-GM-CSF immunoliposomes had better effects on the cytotoxicity of HL60 cells than DHAQ liposomes and DHAQ.The cellar untrastructure was markly destroyed and many apoptotic bodies formed after the treatment. Conclusion:DHAQ-GM-CSF immunoliposomes are successfully prepared and verified,which have targeting cytotoxicity on HL60 cells.The study establishes the foundation for futher investigation.

Objective To establish a method for the identification of Rhizoma Alismatis. Methods HPLC combined with evaporation light scattering detector(ELSD) was applied. Chromatographic column was Macherey-Nagel C18(250 mm?4 mm,10 ?m),acetonitrile -water (4 ∶1) used as mobile phase,flow rate 0.5 mL/min,gas flow rate of ELSD 2.25 L/min and the temperature of drift tube at 70 ℃. Results In the extraction of the authentic Rhizoma Alismatis,both alisol A24-acetate and alisol B23-acetate could be estimated;in the extraction of Rhizoma Alismatis from the market,the alisol A24-acetate could be estimated only;and only alisol B23-acetate could be estimated in the extraction of the standard powder of Rhizoma Alismatis. Conclusion The method is efficient,practical and easy to operate for detecting and identifying Rhizoma Alismatis.

Target-oriented drug delivery systems(TODDS) of Chinese materia medicia(CMM) is ascribed as preparations in which active fractions or single ingredient extracted from CMM or natural medicines are directly delivered with the help of different carriers to target organs,target tissues,and target cells or intracellula.Recent advances of research on TODDSs of CMM classified according to carriers,such as liposomes,microspheres,nanocapsules,and emulsions are reviewed.In addition,due to the specific actions of CMM on intestinal diseases,oral colon targeting drug delivery system is considered substantials for TODDSs of CMM.Domestic researches on TODDS of CMM in its initial stage are limited to formulations using single natural active ingredient as raw material.While the fewer TODDSs using CMM active fractions or formula recipes are underway,which relates to the tremendous difficulties in both establishment of quality standard for CMM active fractions and technology for preparation of CMM.Nevertheless one of the important goals for the development on preparation of CMM is TODDS.

The author first argues that the significance of sectionalizing the medical market is that it is conducive to the discovery of market opportunities; it is conducive to the possession of a greater market share; and it provides a basis for the hospital to identify its target market. Then he sets forth the requirements for sectionalizing the medical market, viz. measurability, accessibility, relative stability, and availability of benefit. Finally he expounds the classification methods when sectionalizing the medical market and the principles for a hospitals selection of a target medical market. These include an appropriate scale and market, a certain purchasing power and endurance, a market not yet completely monopolized by competitors, a market the hospital is capable of managing, a relatively steady social environment, and observance of the procedure for selecting a target medical market.

After the participation of academic library users in reading popularization was analyzed, the participa-tion of Guangxi Health Vocational Technology College Library users in reading popularization marking planning was described with the practical experiences summarized and measures for promoting it put forward.

Objective To evaluate the effect of alpha-lipon and Chinese medicine on diabetic peripheral neuropathy.Methods Sixty patients with diabetic peripheral neuropathy were randomly divided into two groups.The treatment group were treated with alpha-lipon 600 mg/day by intravenous drip and 150 ml Chinese medicine twice daily for two weeks.The control group were treated with alpha-lipon 600 mg daily for two weeks.All patients were evaluated with TSS score and quantitative sensory tests before and after the treatment.Results There were significant differences on the conduction velocities of the tibial nerve((44.36 ±4.05) m/s vs (51.53 ± 4.76) rm/s ; t =9.6 ; P ＜ 0.01),the peroneal nerve ((43.87 ± 3.81) m/s vs (48.46 ±3.91) m/s;t =8.7;P＜0.01) and the sural nerve (42.31 ±5.05) m/s vs (47.76±5.06) m/s;t =7.9;P＜0.01) in the treatment group before and after treatment.For the control group,only significant differences on the conduction velocities of the tibial nerve ((44.78 ± 5.51) m/s vs.(46.18 ± 5.76) m/s ;t =3.5 ;P ＜ 0.01) and the sural nerve (40.76 ±5.86) m/s vs.(43.71 ±5.91) m/s;t =4.2;P ＜0.01) were observed before and after treatment.There was no sigfinicant difference found on the conduction velocities of the peroneal nerve ((44.48 ± 5.21) m/s vs (44.02 ± 5.71) m/s; t =2.7 ; P ＞ 0.05).Compared with the control group,the treatment group had more significant improvement of the conduction velocities of the tibial nerve (t =6.1 ; P ＜0.01),the peroneal nerve(t =4.8 ;P ＜ 0.01) and the sural nerve (t =5.4 ; P ＜ 0.01) after treatment for two weeks.The effective rate was significantly higher in the treatment group than in the control group (80％ (24/31)vs 60％ (18/30) ;x2 =5.01 ;P ＜ 0.05).No patients had severe drug side reaction.Conclusion Treatment of alpha-lipon combined Chinese medicine on patients with diabetic peripheral neuropathy is safe and effective.

It has been well established that interactions between CD40 and CD40L,expressed in a variety of cell types including platelets,vascular endothelial cells,and immune cells,are actively involved in the development of glomerulonephritis,lupus nephritis,and the other kidney disease caused by immunological and unimmunological factors,and that tubular cells expression of CD40 was correlated well with the degree of tubulointerstitial inflammation and injury.The development of therapeutic strategies specifically accommodating CD40/CD40L seem promising against renal glomerular disease.The paper reviews the new advances in this

Since recombinant human growth hormone (rhGH) was licensed for growth hormone deficiency(GHD) in 1985,it has been available in short stature associated with Turner syndrome (TS),being born small for gestational age,idiopathic short stature,chronic renal insufficiency in the last 20 years.The overall safety profile of rhGH continues to be favorable,but careful monitoring for the presence of certain conditions is important both during and after therapy.Reasonable indication,time of therapy and dosage are important to obtain the ideal result and save the expense.Besides,the efforts to improve rhGH therapy and increase compliance have also led to the investigation of extended-release rhGH and less invasive administration routes.Recent advances in the treatment of rhGH are reviewed in details.